MD10G1062300.v1.1

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003235
Physical Location & Seq
Reverse (-)
8403348 .. 8407079
3732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1062300.v1.1.491

Sequence Viewer

Length: 933 bp
ATGTCCACCGTCACCCTGAGCAGTGGCTACGAGATGCCGGTCATCGGTCTCGGCCTTTGGCGTCTGGAGAAGGACGAGCTTAAAGAGGTCATCTTAAATGCTATTAAGATTGGCTATCGCCATTTTGACTGTGCTGCTCATTACAAAAGTGAAGCAGACGTTGGAGAAGCACTTGCAGAAGCATTTAAGACTGGACTTGTTAAGAGGGAAGAACTTTTCATTACCACCAAGATTTGGAATTCAGACCATGGGCATGTGGTGGAGGCCTGTAAGAACAGCCTCGAGAAGCTTCAGATAGATTATCTGGATCTCTACCTGGTTCACTACCCAATGCCCACAAAGCACAATGCAATTGGTAAAACTGCCAGTCTTTTGGGCGAGGATAAGGTGTTGGACATCGATGTAACAATTTCCCTTCAACAAACCTGGGAGGGCATGGAAAAGACCGTCTCTTTGGGCTTAGTTCGCAGCATTGGTCTCAGCAACTATGAGCTCTTTCTAACTAGAGATTGCTTGGCTTACTCCAAAATAAAGCCTGCTGTGAGCCAATTTGAAACCCACCCCTATTTCCAGCGCGACTCTCTCGTCAAATTCTGTATGAAACACGGCGTTCTTCCCACAGCTCACACCCCTCTCGGAGGTGCTGCTGCCAACAAGGATATGTTTGGTTCTGTTTCACCTTTGGATGATCCAGTTCTCAATGATGTGGCTAAGAAATACGGAAAGAGCGTGGCACAAATCTGTCTGAGGTGGGGAATTCAGAGGAAAACAGCAGTGATTCCAAAATCATCGAAAATTCAGCGATTGAAAGAGAATTTGGAGGTTCTTGAATTCCAGCTGAGCGATGAAGACATGCAGCTCATCTACAGTATCGACAGGAAGTATCGTACCAGTCTACCTTCCAAGACTTGGGGCTTAGACGTGTATGCATAA

Protein Analysis

311

Amino Acids

34.9

Weight (kDa)

6.76

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 15 - 292 1.2e-53 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 584
AccB7I CCANNNNNTGG 2 cut(s) 234, 909
AccI GTMKAC 1 cut(s) 895
AccII CGCG 1 cut(s) 576
AclWI GGATC 2 cut(s) 315, 683
AcsI RAATTY 6 cut(s) 238, 590, 756, 795, 814, 830
AcuI CTGAAG 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 1 cut(s) 889
AfiI CCNNNNNNNGG 4 cut(s) 44, 234, 638, 909
AflIII ACRYGT 1 cut(s) 921
AgsI TTSAA 4 cut(s) 419, 554, 808, 830
AjiI CACGTC 1 cut(s) 922
AjnI CCWGG 2 cut(s) 315, 425
AjuI GAANNNNNNNTTGG 4 cut(s) 144, 176, 800, 832
AluBI AGCT 6 cut(s) 79, 289, 493, 623, 838, 859
AluI AGCT 6 cut(s) 79, 289, 493, 623, 838, 859
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 3 cut(s) 53, 454, 482
AlwI GGATC 2 cut(s) 315, 683
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 2 cut(s) 52, 264
ApeKI GCWGC 5 cut(s) 134, 468, 644, 647, 856
ApoI RAATTY 6 cut(s) 238, 590, 756, 795, 814, 830
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
AspLEI GCGC 1 cut(s) 576
AsuHPI GGTGA 2 cut(s) 4, 669
AvaI CYCGRG 1 cut(s) 281
BanII GRGCYC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 5 cut(s) 121, 480, 631, 634, 868
BceAI ACGGC 1 cut(s) 622
BciT130I CCWGG 2 cut(s) 317, 427
BcoDI GTCTC 3 cut(s) 53, 454, 482
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 1 cut(s) 865
BisI GCNGC 5 cut(s) 135, 469, 645, 648, 857
BlpI GCTNAGC 1 cut(s) 839
BlsI GCNGC 5 cut(s) 136, 470, 646, 649, 858
Bme1390I CCNGG 2 cut(s) 317, 427
BmeT110I CYCGRG 1 cut(s) 281
BmgBI CACGTC 1 cut(s) 922
BmrFI CCNGG 2 cut(s) 317, 427
BmsI GCATC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 855
BpmI CTGGAG 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 17
Bpu1102I GCTNAGC 1 cut(s) 839
Bsa29I ATCGAT 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 61
BsaI GGTCTC 2 cut(s) 53, 482
BsaJI CCNNGG 2 cut(s) 247, 426
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 234, 638, 909
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 4 cut(s) 196, 366, 692, 891
BseBI CCWGG 2 cut(s) 317, 427
BseCI ATCGAT 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 247, 426
BseGI GGATG 1 cut(s) 691
BseLI CCNNNNNNNGG 4 cut(s) 44, 234, 638, 909
BseMII CTCAG 4 cut(s) 8, 493, 737, 830
BseNI ACTGG 4 cut(s) 196, 366, 692, 891
BseXI GCAGC 5 cut(s) 121, 480, 631, 634, 868
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 2 cut(s) 54, 266
BshVI ATCGAT 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 495
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 4 cut(s) 44, 234, 638, 909
BsmAI GTCTC 3 cut(s) 53, 454, 482
BsmBI CGTCTC 1 cut(s) 454
BsnI GGCC 2 cut(s) 54, 266
Bso31I GGTCTC 2 cut(s) 53, 482
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 2 cut(s) 307, 688
Bsp1720I GCTNAGC 1 cut(s) 839
Bsp19I CCATGG 1 cut(s) 247
BspANI GGCC 2 cut(s) 54, 266
BspCNI CTCAG 4 cut(s) 9, 492, 738, 831
BspDI ATCGAT 1 cut(s) 399
BspFNI CGCG 1 cut(s) 576
BspPI GGATC 2 cut(s) 315, 683
BspTNI GGTCTC 2 cut(s) 53, 482
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 4 cut(s) 196, 366, 692, 891
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 247, 426
BssMI GATC 2 cut(s) 307, 688
BssNI GRCGYC 1 cut(s) 61
BssT1I CCWWGG 1 cut(s) 247
Bst2UI CCWGG 2 cut(s) 317, 427
Bst4CI ACNGT 4 cut(s) 10, 131, 448, 869
BstACI GRCGYC 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 7 cut(s) 17, 460, 479, 711, 746, 839, 916
BstDSI CCRYGG 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 1 cut(s) 691
BstFNI CGCG 1 cut(s) 576
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 2 cut(s) 310, 691
BstMAI GTCTC 3 cut(s) 53, 454, 482
BstMBI GATC 2 cut(s) 307, 688
BstMWI GCNNNNNNNGC 2 cut(s) 340, 465
BstNI CCWGG 2 cut(s) 317, 427
BstNSI RCATGY 2 cut(s) 257, 856
BstSCI CCNGG 2 cut(s) 315, 425
BstSFI CTRYAG 1 cut(s) 865
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 5 cut(s) 121, 480, 631, 634, 868
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 307
Bsu15I ATCGAT 1 cut(s) 399
BsuRI GGCC 2 cut(s) 54, 266
BsuTUI ATCGAT 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 247
BtgZI GCGATG 1 cut(s) 858
BtrI CACGTC 1 cut(s) 922
BtsCI GGATG 1 cut(s) 691
BtsI GCAGTG 2 cut(s) 28, 780
BtsIMutI CAGTG 2 cut(s) 28, 780
Cac8I GCNNGC 1 cut(s) 537
CfoI GCGC 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 399
CseI GACGC 1 cut(s) 50
CsiI ACCWGGT 1 cut(s) 315
Csp6I GTAC 1 cut(s) 888
CviAII CATG 4 cut(s) 248, 254, 436, 853
CviQI GTAC 1 cut(s) 888
DdeI CTNAG 7 cut(s) 17, 460, 479, 711, 746, 839, 916
DpnI GATC 2 cut(s) 309, 690
DpnII GATC 2 cut(s) 307, 688
DrdI GACNNNNNNGTC 1 cut(s) 584
DseDI GACNNNNNNGTC 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 2 cut(s) 53, 482
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 493
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoRI GAATTC 3 cut(s) 238, 756, 830
EcoRII CCWGG 2 cut(s) 315, 425
EcoT14I CCWWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 454
FaeI CATG 4 cut(s) 251, 257, 439, 856
FaiI YATR 9 cut(s) 249, 255, 437, 489, 599, 662, 854, 927, 931
FatI CATG 4 cut(s) 247, 253, 435, 852
FblI GTMKAC 1 cut(s) 895
Fnu4HI GCNGC 5 cut(s) 135, 469, 645, 648, 857
FokI GGATG 1 cut(s) 698
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 5 cut(s) 135, 469, 645, 648, 857
FspBI CTAG 1 cut(s) 504
GlaI GCGC 1 cut(s) 575
GluI GCNGC 5 cut(s) 135, 469, 645, 648, 857
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 2 cut(s) 54, 266
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 576
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 4 cut(s) 251, 257, 439, 856
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 2 cut(s) 578, 778
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 4, 669
Hpy166II GTNNAC 3 cut(s) 6, 322, 896
Hpy188I TCNGA 5 cut(s) 244, 294, 638, 747, 762
Hpy188III TCNNGA 4 cut(s) 65, 283, 305, 827
Hpy8I GTNNAC 3 cut(s) 6, 322, 896
HpyAV CCTTC 3 cut(s) 64, 425, 909
HpyCH4III ACNGT 4 cut(s) 10, 131, 448, 869
HpyCH4IV ACGT 2 cut(s) 159, 921
HpyCH4V TGCA 4 cut(s) 176, 350, 856, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 340, 465
HpyF3I CTNAG 7 cut(s) 17, 460, 479, 711, 746, 839, 916
HpySE526I ACGT 2 cut(s) 159, 921
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 4 cut(s) 251, 257, 439, 856
HspAI GCGC 1 cut(s) 574
Kzo9I GATC 2 cut(s) 307, 688
Lsp1109I GCAGC 5 cut(s) 121, 480, 631, 634, 868
LweI GCATC 1 cut(s) 24
MabI ACCWGGT 1 cut(s) 315
MaeI CTAG 1 cut(s) 504
MaeII ACGT 2 cut(s) 159, 921
MaeIII GTNAC 2 cut(s) 10, 403
MalI GATC 2 cut(s) 309, 690
MboI GATC 2 cut(s) 307, 688
MboII GAAGA 3 cut(s) 221, 605, 860
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 9 cut(s) 238, 351, 408, 548, 590, 756, 795, 814, 830
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 2 cut(s) 142, 372
Mph1103I ATGCAT 1 cut(s) 931
MseI TTAA 5 cut(s) 81, 95, 105, 186, 201
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 838
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 2 cut(s) 317, 427
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 2 cut(s) 317, 427
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 340, 465
NcoI CCATGG 1 cut(s) 247
NdeII GATC 2 cut(s) 307, 688
NlaIII CATG 4 cut(s) 251, 257, 439, 856
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 2 cut(s) 257, 856
PaeR7I CTCGAG 1 cut(s) 281
PceI AGGCCT 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 726
PfeI GAWTC 1 cut(s) 778
PflMI CCANNNNNTGG 2 cut(s) 234, 909
PkrI GCNGC 5 cut(s) 136, 470, 646, 649, 858
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 2 cut(s) 315, 425
PspGI CCWGG 2 cut(s) 315, 425
PsuI RGATCY 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 838
RsaI GTAC 1 cut(s) 889
RsaNI GTAC 1 cut(s) 888
RseI CAYNNNNRTG 1 cut(s) 252
SacI GAGCTC 1 cut(s) 495
SaqAI TTAA 5 cut(s) 81, 95, 105, 186, 201
SatI GCNGC 5 cut(s) 135, 469, 645, 648, 857
Sau3AI GATC 2 cut(s) 307, 688
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 317, 427
SduI GDGCHC 1 cut(s) 495
SexAI ACCWGGT 1 cut(s) 315
SfaNI GCATC 1 cut(s) 24
SfcI CTRYAG 1 cut(s) 865
Sfr274I CTCGAG 1 cut(s) 281
SlaI CTCGAG 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 9 cut(s) 238, 351, 408, 548, 590, 756, 795, 814, 830
SseBI AGGCCT 1 cut(s) 266
SspMI CTAG 1 cut(s) 504
SstI GAGCTC 1 cut(s) 495
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 315, 425
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 4 cut(s) 10, 131, 448, 869
TaiI ACGT 2 cut(s) 162, 924
TaqI TCGA 4 cut(s) 282, 399, 791, 873
TaqII GACCGA 1 cut(s) 35
TasI AATT 9 cut(s) 238, 351, 408, 548, 590, 756, 795, 814, 830
TfiI GAWTC 1 cut(s) 778
Tru1I TTAA 5 cut(s) 81, 95, 105, 186, 201
Tru9I TTAA 5 cut(s) 81, 95, 105, 186, 201
TscAI CASTG 2 cut(s) 28, 780
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 5 cut(s) 134, 468, 644, 647, 856
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 208, 614, 861
TspGWI ACGGA 1 cut(s) 735
TspRI CASTG 2 cut(s) 28, 780
Van91I CCANNNNNTGG 2 cut(s) 234, 909
XagI CCTNNNNNAGG 1 cut(s) 636
XapI RAATTY 6 cut(s) 238, 590, 756, 795, 814, 830
XceI RCATGY 2 cut(s) 257, 856
XhoI CTCGAG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 895
XspI CTAG 1 cut(s) 504
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.