Rw6G013860

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003235
Physical Location & Seq
Forward (+)
25639074 .. 25640718
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013860.1

Sequence Viewer

Length: 933 bp
ATGGCAATTAGTCTGAATAGTGGGTTCGAGATGCCACTAATGGGTCTGGGTCTTTGGCGCATGGAAAAAGATCAGATCAGACCTCTCATCATCACTGCCATAAAACTTGGCTATCGCCATTTTGACTGCGCTGCCCATTACAAGAATGAAGTAGAAGTTGGGAACGCGCTTGCAGAAGCATTTCGGTCTGGACTTGTCAAGAGGGAGGATGTGTTCATTACCTCGAAGCTTTGGAATTCAGACCATGGACATGTTGTTGAGGCCTGCAGAGACAGCCTGAAGAAGCTTCAGTTGGAGTATCTAGATCTCTACTTGATTCACTTTCCTATAGCCTCTACTCATACTGGAGTTGGTACTACTGGTAGTGTTTTGGATGAGGATAAGATGCTGGACATGGACACCACGATATCCCTGGAAAGTACCTGGCATGATATGGAAAAGCTTGTGTCTCTGGGATTAGTTCGAAGCATTGGAATCAGCAACTATGACATCTTTCTAACAAGAGATTGCTTAGCTTACTCCAAAATAAAGCCTGCAGTAAATCAGTTTGAAAGTCACCCATACTTCCAGCGCGAATCTCTCATCAAATTCTGTAGGAAGCACGGAATATGCGCTACAGCTCATACCCCTCTAGGAGGCTCTGTGGCCAACACTAAGGACTTTGGTTCAATTTCTTGTTTGGATGATCCAATCCTTAAGGGTTTGGCTAAGAAGTATGAAAAGAGTGCAGCACAGATTGTTCTCAGGTGGGGAATACAAAGGAATATAGCAGTCATTCCAAAGACCTCAAAAGTTGAGAGATTGAAAGAGAATCTGCAAGTTCTTGATGACTTCAAGCTGAGCAAAGAGGACATGGACCTCATCAAAACCATAGATAGGAAATACAGGACCAATCCGGCTTCCAAGAGTTGGGGCCTCGATGTCTATGCATAA

Protein Analysis

310

Amino Acids

35.02

Weight (kDa)

8.11

Isoelectric Point (pI)

25.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 14 - 292 8.8e-53 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 909
AccII CGCG 2 cut(s) 167, 573
AclWI GGATC 1 cut(s) 680
AcoI YGGCCR 1 cut(s) 645
AcsI RAATTY 2 cut(s) 235, 587
AcuI CTGAAG 2 cut(s) 272, 299
AfaI GTAC 2 cut(s) 355, 421
AfiI CCNNNNNNNGG 4 cut(s) 41, 635, 876, 909
AflII CTTAAG 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 4 cut(s) 551, 669, 805, 835
AjnI CCWGG 2 cut(s) 411, 422
AjuI GAANNNNNNNTTGG 4 cut(s) 141, 173, 275, 307
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 6 cut(s) 229, 286, 442, 515, 620, 838
AluI AGCT 6 cut(s) 229, 286, 442, 515, 620, 838
Alw26I GTCTC 2 cut(s) 264, 453
AlwI GGATC 1 cut(s) 680
AoxI GGCC 3 cut(s) 261, 645, 913
ApeKI GCWGC 2 cut(s) 131, 728
ApoI RAATTY 2 cut(s) 235, 587
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 5 cut(s) 60, 131, 169, 573, 614
AspS9I GGNCC 3 cut(s) 856, 888, 913
AsuHPI GGTGA 1 cut(s) 548
AsuII TTCGAA 1 cut(s) 463
AvaII GGWCC 2 cut(s) 856, 888
BalI TGGCCA 1 cut(s) 647
BbvI GCAGC 2 cut(s) 118, 740
BcgI CGANNNNNNTGC 1 cut(s) 908
BciT130I CCWGG 2 cut(s) 413, 424
BcoDI GTCTC 2 cut(s) 264, 453
BfaI CTAG 2 cut(s) 302, 632
BfmI CTRYAG 5 cut(s) 265, 327, 534, 592, 615
BfrI CTTAAG 1 cut(s) 695
BglII AGATCT 1 cut(s) 304
BisI GCNGC 2 cut(s) 132, 729
BlpI GCTNAGC 2 cut(s) 511, 839
BlsI GCNGC 2 cut(s) 133, 730
Bme1390I CCNGG 2 cut(s) 413, 424
Bme18I GGWCC 2 cut(s) 856, 888
BmgT120I GGNCC 3 cut(s) 856, 888, 913
BmiI GGNNCC 1 cut(s) 914
BmrFI CCNGG 2 cut(s) 413, 424
BmsI GCATC 2 cut(s) 21, 375
BpmI CTGGAG 1 cut(s) 366
Bpu1102I GCTNAGC 2 cut(s) 511, 839
Bpu14I TTCGAA 1 cut(s) 463
BsaJI CCNNGG 2 cut(s) 244, 411
BsaXI ACNNNNNCTCC 4 cut(s) 197, 227, 627, 657
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 635, 876, 909
Bse1I ACTGG 2 cut(s) 349, 364
BseBI CCWGG 2 cut(s) 413, 424
BseDI CCNNGG 2 cut(s) 244, 411
BseGI GGATG 3 cut(s) 214, 379, 688
BseLI CCNNNNNNNGG 4 cut(s) 41, 635, 876, 909
BseMII CTCAG 2 cut(s) 757, 830
BseNI ACTGG 2 cut(s) 349, 364
BseXI GCAGC 2 cut(s) 118, 740
BsgI GTGCAG 1 cut(s) 747
Bsh1236I CGCG 2 cut(s) 167, 573
BshFI GGCC 3 cut(s) 263, 647, 915
BsiSI CCGG 1 cut(s) 896
BslI CCNNNNNNNGG 4 cut(s) 41, 635, 876, 909
BsmAI GTCTC 2 cut(s) 264, 453
BsnI GGCC 3 cut(s) 263, 647, 915
Bsp119I TTCGAA 1 cut(s) 463
Bsp143I GATC 4 cut(s) 70, 75, 304, 685
Bsp1720I GCTNAGC 2 cut(s) 511, 839
Bsp19I CCATGG 1 cut(s) 244
BspANI GGCC 3 cut(s) 263, 647, 915
BspCNI CTCAG 2 cut(s) 756, 831
BspFNI CGCG 2 cut(s) 167, 573
BspLI GGNNCC 1 cut(s) 914
BspMAI CTGCAG 2 cut(s) 269, 538
BspPI GGATC 1 cut(s) 680
BspT104I TTCGAA 1 cut(s) 463
BspTI CTTAAG 1 cut(s) 695
BsrI ACTGG 2 cut(s) 349, 364
BssECI CCNNGG 2 cut(s) 244, 411
BssMI GATC 4 cut(s) 70, 75, 304, 685
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 2 cut(s) 413, 424
BstAFI CTTAAG 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 463
BstC8I GCNNGC 3 cut(s) 171, 265, 534
BstDEI CTNAG 5 cut(s) 511, 654, 708, 743, 839
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 3 cut(s) 214, 379, 688
BstFNI CGCG 2 cut(s) 167, 573
BstHHI GCGC 5 cut(s) 60, 131, 169, 573, 614
BstKTI GATC 4 cut(s) 73, 78, 307, 688
BstMAI GTCTC 2 cut(s) 264, 453
BstMBI GATC 4 cut(s) 70, 75, 304, 685
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstNI CCWGG 2 cut(s) 413, 424
BstNSI RCATGY 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 411, 422
BstSFI CTRYAG 5 cut(s) 265, 327, 534, 592, 615
BstUI CGCG 2 cut(s) 167, 573
BstV1I GCAGC 2 cut(s) 118, 740
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuRI GGCC 3 cut(s) 263, 647, 915
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 214, 379, 688
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 3 cut(s) 171, 265, 534
CfoI GCGC 5 cut(s) 60, 131, 169, 573, 614
Cfr13I GGNCC 3 cut(s) 856, 888, 913
Csp6I GTAC 2 cut(s) 354, 420
CviAII CATG 6 cut(s) 61, 245, 251, 394, 428, 853
CviQI GTAC 2 cut(s) 354, 420
DdeI CTNAG 5 cut(s) 511, 654, 708, 743, 839
DpnI GATC 4 cut(s) 72, 77, 306, 687
DpnII GATC 4 cut(s) 70, 75, 304, 685
EaeI YGGCCR 1 cut(s) 645
Eco130I CCWWGG 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 263
Eco32I GATATC 1 cut(s) 408
Eco47I GGWCC 2 cut(s) 856, 888
Eco57I CTGAAG 2 cut(s) 272, 299
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 913
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 411, 422
EcoRV GATATC 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 244
EcoT22I ATGCAT 1 cut(s) 931
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 6 cut(s) 64, 248, 254, 397, 431, 856
FatI CATG 6 cut(s) 60, 244, 250, 393, 427, 852
Fnu4HI GCNGC 2 cut(s) 132, 729
FokI GGATG 3 cut(s) 221, 386, 695
Fsp4HI GCNGC 2 cut(s) 132, 729
FspBI CTAG 2 cut(s) 302, 632
GlaI GCGC 5 cut(s) 59, 130, 168, 572, 613
GluI GCNGC 2 cut(s) 132, 729
GsuI CTGGAG 1 cut(s) 366
HaeIII GGCC 3 cut(s) 263, 647, 915
HapII CCGG 1 cut(s) 896
HhaI GCGC 5 cut(s) 60, 131, 169, 573, 614
Hin1II CATG 6 cut(s) 64, 248, 254, 397, 431, 856
Hin6I GCGC 5 cut(s) 58, 129, 167, 571, 612
HinP1I GCGC 5 cut(s) 58, 129, 167, 571, 612
HindIII AAGCTT 3 cut(s) 227, 284, 440
HinfI GANTC 4 cut(s) 316, 474, 575, 811
HpaII CCGG 1 cut(s) 896
HphI GGTGA 1 cut(s) 548
Hpy188I TCNGA 4 cut(s) 15, 75, 80, 241
Hpy188III TCNNGA 5 cut(s) 28, 189, 199, 302, 824
HpyCH4V TGCA 6 cut(s) 173, 267, 536, 728, 817, 929
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 5 cut(s) 511, 654, 708, 743, 839
Hsp92II CATG 6 cut(s) 64, 248, 254, 397, 431, 856
HspAI GCGC 5 cut(s) 58, 129, 167, 571, 612
Kzo9I GATC 4 cut(s) 70, 75, 304, 685
Lsp1109I GCAGC 2 cut(s) 118, 740
LweI GCATC 2 cut(s) 21, 375
MaeI CTAG 2 cut(s) 302, 632
MaeIII GTNAC 1 cut(s) 554
MalI GATC 4 cut(s) 72, 77, 306, 687
MboI GATC 4 cut(s) 70, 75, 304, 685
MboII GAAGA 1 cut(s) 292
MflI RGATCY 1 cut(s) 304
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 4 cut(s) 6, 235, 587, 669
MluNI TGGCCA 1 cut(s) 647
MmeI TCCRAC 1 cut(s) 273
Mox20I TGGCCA 1 cut(s) 647
Mph1103I ATGCAT 1 cut(s) 931
MroXI GAANNNNTTC 1 cut(s) 180
MscI TGGCCA 1 cut(s) 647
MseI TTAA 1 cut(s) 696
MslI CAYNNNNRTG 1 cut(s) 249
Msp20I TGGCCA 1 cut(s) 647
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 1 cut(s) 896
MspR9I CCNGG 2 cut(s) 413, 424
MvaI CCWGG 2 cut(s) 413, 424
MvnI CGCG 2 cut(s) 167, 573
MwoI GCNNNNNNNGC 1 cut(s) 273
NcoI CCATGG 1 cut(s) 244
NdeII GATC 4 cut(s) 70, 75, 304, 685
NlaIII CATG 6 cut(s) 64, 248, 254, 397, 431, 856
NlaIV GGNNCC 1 cut(s) 914
NmuCI GTSAC 1 cut(s) 554
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 1 cut(s) 254
NspV TTCGAA 1 cut(s) 463
PceI AGGCCT 1 cut(s) 263
PciI ACATGT 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 4 cut(s) 316, 474, 575, 811
PflMI CCANNNNNTGG 1 cut(s) 909
PkrI GCNGC 2 cut(s) 133, 730
PscI ACATGT 1 cut(s) 250
Psp6I CCWGG 2 cut(s) 411, 422
PspGI CCWGG 2 cut(s) 411, 422
PspN4I GGNNCC 1 cut(s) 914
PspPI GGNCC 3 cut(s) 856, 888, 913
PstI CTGCAG 2 cut(s) 269, 538
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 2 cut(s) 355, 421
RsaNI GTAC 2 cut(s) 354, 420
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 1 cut(s) 696
SatI GCNGC 2 cut(s) 132, 729
Sau3AI GATC 4 cut(s) 70, 75, 304, 685
Sau96I GGNCC 3 cut(s) 856, 888, 913
ScrFI CCNGG 2 cut(s) 413, 424
SfaNI GCATC 2 cut(s) 21, 375
SfcI CTRYAG 5 cut(s) 265, 327, 534, 592, 615
SfuI TTCGAA 1 cut(s) 463
SinI GGWCC 2 cut(s) 856, 888
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
Sse9I AATT 4 cut(s) 6, 235, 587, 669
SseBI AGGCCT 1 cut(s) 263
SspMI CTAG 2 cut(s) 302, 632
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 411, 422
StyI CCWWGG 1 cut(s) 244
TaqI TCGA 4 cut(s) 27, 224, 463, 918
TaqII GACCGA 1 cut(s) 174
TasI AATT 4 cut(s) 6, 235, 587, 669
TfiI GAWTC 4 cut(s) 316, 474, 575, 811
Tru1I TTAA 1 cut(s) 696
Tru9I TTAA 1 cut(s) 696
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 2 cut(s) 131, 728
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 3 cut(s) 162, 205, 732
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 1 cut(s) 100
Van91I CCANNNNNTGG 1 cut(s) 909
Vha464I CTTAAG 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 856, 888
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 2 cut(s) 235, 587
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 1 cut(s) 254
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 2 cut(s) 302, 632
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.