MD02G1264100.v1.1

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003235
Physical Location & Seq
Reverse (-)
31799676 .. 31803167
3492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1264100.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCGATAACACTCAACAGTGGGTTCAACATGCCAGTAGTGGGTCTGGGTGTTTGGCGCATGGAAGGAAAAGAAATCAGAGACCTCGTTCTCAATGCCCTCAACCTTGGCTATCGCCATTTCGATTGCGCTGCTGATTACAAGAATGAACCAGAAGTTGGGGAGGCACTTGCAGAAGCATTTTCGACTGGGCTTGTTAGGCGAGAGGATATCTTCATCACCACCAAGCTTTGGAATTCGGATCATGGACATGTTGTAGAGGCCTGTAAAGACAGTCTGAAGAAGCTTCGGTTGGATTATCTGGATCTGTACCTTGTTCACTTTCCTGTTGCCACCAAGCATACCGGAGTTGGTACGACTGGCAGTGCTTTGGATGAGGATGGGGTGCTAGAAATAGACACAACCATATCTTTGGAAACTACCTGGCATGCTATGGAAGAGCTGGTCTCTATGGGTTTAGTTCGTAGCATTGGAATTAGTAACTATGATATCTTTCTGACGAGAGATTGCTTAGCTTATTCCGAAGTGAAGCCCGCAGTGAATCAGATTGAGACTCACCCATACTTCCAACGTGAATCTCTTGTCAAATTCTGTCAGAAGCATGGCATCTGTGTCACTGCCCACACTCCTCTTGGAGGTGCTGTGGCCAACAGCGAGTGGTTTGGTTCAGTTTCATGTCTAGACGATCCAGTTCTTAAGAATCTGGCTGAGAAATACAAAAAGACTGCTGCCCAAGTTGTTCTAAGGTGGGGCATCCAACGGAACACAGTTGTCATCCCGAAGACGTCAAAACTTGAGAGATTGAAAGAGAATTTTCAAGTTTTTGACTTTGAGCTCACCAAAGAGGACATGGACCTGATTGCAAAGGTAAACAGGAAACACCGGACAAATCTACCTTGCAAGTTCTGGGGCGTTGATCTGTTTGAATAG

Protein Analysis

310

Amino Acids

34.86

Weight (kDa)

5.84

Isoelectric Point (pI)

24.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 15 - 291 4e-55 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 788
AccB7I CCANNNNNTGG 2 cut(s) 158, 231
AciI CCGC 1 cut(s) 534
AclWI GGATC 3 cut(s) 249, 312, 680
AcoI YGGCCR 1 cut(s) 645
AcsI RAATTY 3 cut(s) 235, 587, 811
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 785
AfaI GTAC 2 cut(s) 311, 355
AfiI CCNNNNNNNGG 4 cut(s) 41, 158, 231, 635
AflII CTTAAG 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 4 cut(s) 28, 805, 818, 926
AjnI CCWGG 1 cut(s) 422
AjuI GAANNNNNNNTTGG 4 cut(s) 141, 173, 275, 307
AluBI AGCT 5 cut(s) 229, 286, 442, 515, 835
AluI AGCT 5 cut(s) 229, 286, 442, 515, 835
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 3 cut(s) 75, 451, 545
AlwI GGATC 3 cut(s) 249, 312, 680
AoxI GGCC 2 cut(s) 261, 645
ApeKI GCWGC 2 cut(s) 131, 728
ApoI RAATTY 3 cut(s) 235, 587, 811
AspLEI GCGC 2 cut(s) 60, 131
AspS9I GGNCC 1 cut(s) 853
AsuHPI GGTGA 3 cut(s) 211, 548, 829
AvaII GGWCC 1 cut(s) 853
BalI TGGCCA 1 cut(s) 647
BanII GRGCYC 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 788
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 2 cut(s) 118, 715
BccI CCATC 1 cut(s) 374
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 424
BcoDI GTCTC 3 cut(s) 75, 451, 545
BfaI CTAG 2 cut(s) 389, 680
BfrI CTTAAG 1 cut(s) 695
BisI GCNGC 2 cut(s) 132, 729
BlpI GCTNAGC 1 cut(s) 511
BlsI GCNGC 2 cut(s) 133, 730
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 1 cut(s) 853
BmrFI CCNGG 1 cut(s) 424
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 2 cut(s) 615, 762
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 788
BplI GAGNNNNNCTC 2 cut(s) 431, 463
Bpu1102I GCTNAGC 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 785
BsaI GGTCTC 2 cut(s) 75, 451
BsaJI CCNNGG 1 cut(s) 106
BsaWI WCCGGW 2 cut(s) 344, 882
BsaXI ACNNNNNCTCC 2 cut(s) 627, 657
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 158, 231, 635
Bse1I ACTGG 4 cut(s) 35, 193, 364, 689
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 4 cut(s) 379, 385, 753, 774
BseLI CCNNNNNNNGG 4 cut(s) 41, 158, 231, 635
BseMII CTCAG 1 cut(s) 699
BseNI ACTGG 4 cut(s) 35, 193, 364, 689
BseRI GAGGAG 1 cut(s) 618
BseXI GCAGC 2 cut(s) 118, 715
BshFI GGCC 2 cut(s) 263, 647
BsiHKAI GWGCWC 1 cut(s) 837
BsiSI CCGG 2 cut(s) 345, 883
BslI CCNNNNNNNGG 4 cut(s) 41, 158, 231, 635
BsmAI GTCTC 3 cut(s) 75, 451, 545
BsnI GGCC 2 cut(s) 263, 647
Bso31I GGTCTC 2 cut(s) 75, 451
Bsp1286I GDGCHC 1 cut(s) 837
Bsp143I GATC 4 cut(s) 241, 304, 685, 916
Bsp1720I GCTNAGC 1 cut(s) 511
BspACI CCGC 1 cut(s) 534
BspANI GGCC 2 cut(s) 263, 647
BspCNI CTCAG 1 cut(s) 700
BspPI GGATC 3 cut(s) 249, 312, 680
BspQI GCTCTTC 1 cut(s) 432
BspTI CTTAAG 1 cut(s) 695
BspTNI GGTCTC 2 cut(s) 75, 451
BsrI ACTGG 4 cut(s) 35, 193, 364, 689
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 4 cut(s) 241, 304, 685, 916
BssNI GRCGYC 1 cut(s) 785
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 3 cut(s) 20, 275, 769
Bst6I CTCTTC 1 cut(s) 432
BstACI GRCGYC 1 cut(s) 785
BstAFI CTTAAG 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 429, 534
BstDEI CTNAG 3 cut(s) 511, 708, 743
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 4 cut(s) 379, 385, 753, 774
BstHHI GCGC 2 cut(s) 60, 131
BstKTI GATC 4 cut(s) 244, 307, 688, 919
BstMAI GTCTC 3 cut(s) 75, 451, 545
BstMBI GATC 4 cut(s) 241, 304, 685, 916
BstMWI GCNNNNNNNGC 1 cut(s) 199
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 3 cut(s) 34, 254, 431
BstSCI CCNGG 1 cut(s) 422
BstV1I GCAGC 2 cut(s) 118, 715
BstV2I GAAGAC 1 cut(s) 788
BstX2I RGATCY 1 cut(s) 304
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 1 cut(s) 304
BsuRI GGCC 2 cut(s) 263, 647
BtsCI GGATG 4 cut(s) 379, 385, 753, 774
BtsI GCAGTG 3 cut(s) 370, 543, 615
BtsIMutI CAGTG 4 cut(s) 25, 370, 543, 615
Cac8I GCNNGC 2 cut(s) 429, 534
CfoI GCGC 2 cut(s) 60, 131
Cfr13I GGNCC 1 cut(s) 853
Csp6I GTAC 2 cut(s) 310, 354
CviAII CATG 8 cut(s) 31, 61, 245, 251, 428, 602, 675, 850
CviQI GTAC 2 cut(s) 310, 354
DdeI CTNAG 3 cut(s) 511, 708, 743
DpnI GATC 4 cut(s) 243, 306, 687, 918
DpnII GATC 4 cut(s) 241, 304, 685, 916
EaeI YGGCCR 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 1 cut(s) 106
Eco147I AGGCCT 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 837
Eco31I GGTCTC 2 cut(s) 75, 451
Eco32I GATATC 2 cut(s) 211, 490
Eco47I GGWCC 1 cut(s) 853
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 835
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 422
EcoRV GATATC 2 cut(s) 211, 490
EcoT14I CCWWGG 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 837
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 8 cut(s) 34, 64, 248, 254, 431, 605, 678, 853
FatI CATG 8 cut(s) 30, 60, 244, 250, 427, 601, 674, 849
FauI CCCGC 1 cut(s) 541
Fnu4HI GCNGC 2 cut(s) 132, 729
FokI GGATG 4 cut(s) 386, 392, 740, 761
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 2 cut(s) 132, 729
FspBI CTAG 2 cut(s) 389, 680
GlaI GCGC 2 cut(s) 59, 130
GluI GCNGC 2 cut(s) 132, 729
HaeIII GGCC 2 cut(s) 263, 647
HapII CCGG 2 cut(s) 345, 883
HhaI GCGC 2 cut(s) 60, 131
Hin1I GRCGYC 1 cut(s) 785
Hin1II CATG 8 cut(s) 34, 64, 248, 254, 431, 605, 678, 853
Hin6I GCGC 2 cut(s) 58, 129
HinP1I GCGC 2 cut(s) 58, 129
HindIII AAGCTT 2 cut(s) 227, 284
HinfI GANTC 4 cut(s) 541, 553, 575, 700
HpaII CCGG 2 cut(s) 345, 883
HphI GGTGA 3 cut(s) 211, 548, 829
Hpy166II GTNNAC 2 cut(s) 319, 871
Hpy188I TCNGA 7 cut(s) 80, 241, 279, 498, 523, 546, 597
Hpy188III TCNNGA 3 cut(s) 302, 680, 778
Hpy8I GTNNAC 2 cut(s) 319, 871
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 3 cut(s) 20, 275, 769
HpyCH4IV ACGT 2 cut(s) 571, 785
HpyCH4V TGCA 3 cut(s) 173, 863, 900
HpyF10VI GCNNNNNNNGC 1 cut(s) 199
HpyF3I CTNAG 3 cut(s) 511, 708, 743
HpySE526I ACGT 2 cut(s) 571, 785
Hsp92I GRCGYC 1 cut(s) 785
Hsp92II CATG 8 cut(s) 34, 64, 248, 254, 431, 605, 678, 853
HspAI GCGC 2 cut(s) 58, 129
Kzo9I GATC 4 cut(s) 241, 304, 685, 916
LguI GCTCTTC 1 cut(s) 432
Lsp1109I GCAGC 2 cut(s) 118, 715
LweI GCATC 2 cut(s) 615, 762
MaeI CTAG 2 cut(s) 389, 680
MaeII ACGT 2 cut(s) 571, 785
MaeIII GTNAC 2 cut(s) 479, 613
MalI GATC 4 cut(s) 243, 306, 687, 918
MboI GATC 4 cut(s) 241, 304, 685, 916
MboII GAAGA 4 cut(s) 205, 292, 449, 793
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 837
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 4 cut(s) 235, 474, 587, 811
MluNI TGGCCA 1 cut(s) 647
MlyI GAGTC 1 cut(s) 547
MmeI TCCRAC 3 cut(s) 273, 592, 781
MnlI CCTC 9 cut(s) 95, 110, 157, 199, 253, 370, 629, 639, 838
Mox20I TGGCCA 1 cut(s) 647
MscI TGGCCA 1 cut(s) 647
MseI TTAA 1 cut(s) 696
MslI CAYNNNNRTG 1 cut(s) 249
Msp20I TGGCCA 1 cut(s) 647
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 2 cut(s) 345, 883
MspR9I CCNGG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 424
MwoI GCNNNNNNNGC 1 cut(s) 199
NdeII GATC 4 cut(s) 241, 304, 685, 916
NlaIII CATG 8 cut(s) 34, 64, 248, 254, 431, 605, 678, 853
NmuCI GTSAC 1 cut(s) 613
NspI RCATGY 3 cut(s) 34, 254, 431
PaeI GCATGC 1 cut(s) 431
PceI AGGCCT 1 cut(s) 263
PciI ACATGT 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 432
PfeI GAWTC 3 cut(s) 541, 575, 700
PflMI CCANNNNNTGG 2 cut(s) 158, 231
PkrI GCNGC 2 cut(s) 133, 730
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PscI ACATGT 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspPI GGNCC 1 cut(s) 853
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 2 cut(s) 311, 355
RsaNI GTAC 2 cut(s) 310, 354
RseI CAYNNNNRTG 1 cut(s) 249
SacI GAGCTC 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 1 cut(s) 696
SatI GCNGC 2 cut(s) 132, 729
Sau3AI GATC 4 cut(s) 241, 304, 685, 916
Sau96I GGNCC 1 cut(s) 853
SchI GAGTC 1 cut(s) 547
ScrFI CCNGG 1 cut(s) 424
SduI GDGCHC 1 cut(s) 837
SfaNI GCATC 2 cut(s) 615, 762
SinI GGWCC 1 cut(s) 853
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 695, 794
SmoI CTYRAG 2 cut(s) 695, 794
SphI GCATGC 1 cut(s) 431
Sse9I AATT 4 cut(s) 235, 474, 587, 811
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 1 cut(s) 534
SspMI CTAG 2 cut(s) 389, 680
SstI GAGCTC 1 cut(s) 837
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 3 cut(s) 20, 275, 769
TaiI ACGT 2 cut(s) 574, 788
TaqI TCGA 2 cut(s) 123, 185
TasI AATT 4 cut(s) 235, 474, 587, 811
TfiI GAWTC 3 cut(s) 541, 575, 700
Tru1I TTAA 1 cut(s) 696
Tru9I TTAA 1 cut(s) 696
TscAI CASTG 4 cut(s) 25, 370, 543, 622
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 2 cut(s) 131, 728
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 3 cut(s) 162, 205, 663
TspGWI ACGGA 1 cut(s) 775
TspRI CASTG 4 cut(s) 25, 370, 543, 622
Van91I CCANNNNNTGG 2 cut(s) 158, 231
Vha464I CTTAAG 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 853
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 3 cut(s) 235, 587, 811
XbaI TCTAGA 1 cut(s) 679
XceI RCATGY 3 cut(s) 34, 254, 431
XcmI CCANNNNNNNNNTGG 2 cut(s) 629, 847
XspI CTAG 2 cut(s) 389, 680
ZraI GACGTC 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.