MD05G1054800.v1.1

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003235
Physical Location & Seq
Reverse (-)
9388132 .. 9392145
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1054800.v1.1.491

Sequence Viewer

Length: 915 bp
ATGTCCACCGTTATACTCAGCAGTGGCTACGAGATGCCGGTCATCGGCCTCGGCCTTTGGCGTCTGGAGAAGAACGAGCTCAAAGACGTCATCTTAAATGCTATTAAGCTTGGCTATCGCCATTTTGACTGTGCTGCTCATTACAAGAGTGAAACAGACGTTGGGGAAGTATTTGCAGAAGCATTTAAGATTGGACTTGTCAAGAGGGAAGAACTTTTCATTAGCACCAAGATTTGGAATTCAGACCATGGGTATGAGGTTGAGGCCTGTAAAAACAGCCTCAAGAAGCTTCAGCTAGATTATCTGGATCTCTACCTAGTTCACTACCCATTGCCCACGAAGCATAATGCCATTGGTCAAACTGCCAGTCTTATGGGCAAGGACAAGGTGCTGGACATAGATGTAACAATTTCCCTTCAACAAACCTGGGAGGGGACATGGAAAAGACTGTCTCTTTGGGCTTACAACTACGACCTCTTTCTGACCAGAGATTGCTTGGCTTACTCCAAAATAAAGCCTGCAGTGAGCCAATTTGAAACTCACCCCTACTTCCAGCGCGACTCTCTCGTCAAATTCTGCTTGAAACACGGTGTTCTTCCCACAGCTCACACCCCTCTCGGAGGTGCTGCGGCCAACAAGGATATGTTTGGTTCAATTTCACCTTTGGATGATCCAGTTCTCAATAATGTGGCTAAGAAGTACGGAAAGAGTGTGGCACAAATCTGTCTGAGGTGGGGAATTCAAAGGAAAACAGCAGTGATTCCAAAGTCATCAAAAATTAAGAGATTGAAAGAGAATTTGGAGGTTCTTGAATTCCAGCTGAGTCATGAAGACATGGAGGTCATAAACACTATAGACAGGAAATGTCGTACCAGTCTACCTTCCAAGACTTGGGGCTTAGATGTGTATGCATAA

Protein Analysis

305

Amino Acids

34.57

Weight (kDa)

8.7

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 15 - 286 6.2e-41 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 566, 839
AatII GACGTC 1 cut(s) 90
AccB7I CCANNNNNTGG 2 cut(s) 234, 891
AccI GTMKAC 1 cut(s) 877
AccII CGCG 1 cut(s) 558
AciI CCGC 1 cut(s) 629
AclWI GGATC 2 cut(s) 315, 665
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 5 cut(s) 238, 572, 738, 796, 812
AcuI CTGAAG 1 cut(s) 275
AcyI GRCGYC 2 cut(s) 61, 87
AfaI GTAC 2 cut(s) 701, 871
AfiI CCNNNNNNNGG 5 cut(s) 44, 234, 432, 620, 891
AgsI TTSAA 7 cut(s) 419, 536, 583, 654, 743, 790, 812
AjnI CCWGG 1 cut(s) 425
AjuI GAANNNNNNNTTGG 4 cut(s) 144, 176, 782, 814
AluBI AGCT 6 cut(s) 79, 109, 289, 295, 605, 820
AluI AGCT 6 cut(s) 79, 109, 289, 295, 605, 820
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 2 cut(s) 315, 665
AoxI GGCC 4 cut(s) 46, 52, 264, 630
ApeKI GCWGC 2 cut(s) 134, 626
ApoI RAATTY 5 cut(s) 238, 572, 738, 796, 812
ArsI GACNNNNNNTTYG 4 cut(s) 352, 384, 438, 470
AspLEI GCGC 1 cut(s) 558
AsuHPI GGTGA 2 cut(s) 533, 651
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 121, 613
BciT130I CCWGG 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 2 cut(s) 296, 317
BfmI CTRYAG 2 cut(s) 519, 852
BisI GCNGC 3 cut(s) 135, 627, 630
BlsI GCNGC 3 cut(s) 136, 628, 631
Bme1390I CCNGG 1 cut(s) 427
BmrFI CCNGG 1 cut(s) 427
BmsI GCATC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 266
BsaHI GRCGYC 2 cut(s) 61, 87
BsaJI CCNNGG 3 cut(s) 49, 247, 426
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 234, 432, 620, 891
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 3 cut(s) 366, 674, 873
Bse3DI GCAATG 1 cut(s) 329
BseBI CCWGG 1 cut(s) 427
BseDI CCNNGG 3 cut(s) 49, 247, 426
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 5 cut(s) 44, 234, 432, 620, 891
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 3 cut(s) 31, 719, 812
BseNI ACTGG 3 cut(s) 366, 674, 873
BseXI GCAGC 2 cut(s) 121, 613
Bsh1236I CGCG 1 cut(s) 558
BshFI GGCC 4 cut(s) 48, 54, 266, 632
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 5 cut(s) 44, 234, 432, 620, 891
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 4 cut(s) 48, 54, 266, 632
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 2 cut(s) 307, 670
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 1 cut(s) 629
BspANI GGCC 4 cut(s) 48, 54, 266, 632
BspCNI CTCAG 3 cut(s) 30, 720, 813
BspFNI CGCG 1 cut(s) 558
BspHI TCATGA 1 cut(s) 826
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 2 cut(s) 315, 665
BsrDI GCAATG 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 3 cut(s) 366, 674, 873
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 49, 247, 426
BssMI GATC 2 cut(s) 307, 670
BssNI GRCGYC 2 cut(s) 61, 87
BssT1I CCWWGG 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 427
Bst4CI ACNGT 4 cut(s) 10, 131, 450, 590
BstACI GRCGYC 2 cut(s) 61, 87
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 5 cut(s) 17, 693, 728, 821, 898
BstDSI CCRYGG 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 618
BstF5I GGATG 1 cut(s) 673
BstFNI CGCG 1 cut(s) 558
BstHHI GCGC 1 cut(s) 558
BstKTI GATC 2 cut(s) 310, 673
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 2 cut(s) 307, 670
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 1 cut(s) 427
BstSCI CCNGG 1 cut(s) 425
BstSFI CTRYAG 2 cut(s) 519, 852
BstUI CGCG 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 121, 613
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 4 cut(s) 48, 54, 266, 632
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 1 cut(s) 673
BtsI GCAGTG 3 cut(s) 28, 528, 762
BtsIMutI CAGTG 3 cut(s) 28, 528, 762
Cac8I GCNNGC 1 cut(s) 519
CciI TCATGA 1 cut(s) 826
CfoI GCGC 1 cut(s) 558
Cfr10I RCCGGY 1 cut(s) 37
CseI GACGC 1 cut(s) 50
Csp6I GTAC 2 cut(s) 700, 870
CviAII CATG 4 cut(s) 248, 438, 827, 835
CviQI GTAC 2 cut(s) 700, 870
DdeI CTNAG 5 cut(s) 17, 693, 728, 821, 898
DpnI GATC 2 cut(s) 309, 672
DpnII GATC 2 cut(s) 307, 670
DrdI GACNNNNNNGTC 2 cut(s) 566, 839
DseDI GACNNNNNNGTC 2 cut(s) 566, 839
EaeI YGGCCR 1 cut(s) 630
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 618
EcoRI GAATTC 3 cut(s) 238, 738, 812
EcoRII CCWGG 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 913
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 247
FaeI CATG 4 cut(s) 251, 441, 830, 838
FaqI GGGAC 1 cut(s) 448
FatI CATG 4 cut(s) 247, 437, 826, 834
FblI GTMKAC 1 cut(s) 877
Fnu4HI GCNGC 3 cut(s) 135, 627, 630
FokI GGATG 1 cut(s) 680
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 3 cut(s) 135, 627, 630
FspBI CTAG 2 cut(s) 296, 317
GlaI GCGC 1 cut(s) 557
GluI GCNGC 3 cut(s) 135, 627, 630
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 4 cut(s) 48, 54, 266, 632
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 558
Hin1I GRCGYC 2 cut(s) 61, 87
Hin1II CATG 4 cut(s) 251, 441, 830, 838
Hin6I GCGC 1 cut(s) 556
HinP1I GCGC 1 cut(s) 556
HindIII AAGCTT 2 cut(s) 107, 287
HinfI GANTC 3 cut(s) 560, 760, 823
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 533, 651
Hpy166II GTNNAC 3 cut(s) 6, 322, 878
Hpy188I TCNGA 4 cut(s) 244, 483, 620, 729
Hpy188III TCNNGA 6 cut(s) 65, 202, 283, 305, 809, 827
Hpy8I GTNNAC 3 cut(s) 6, 322, 878
HpyAV CCTTC 2 cut(s) 425, 891
HpyCH4III ACNGT 4 cut(s) 10, 131, 450, 590
HpyCH4IV ACGT 2 cut(s) 87, 159
HpyCH4V TGCA 3 cut(s) 176, 521, 911
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 5 cut(s) 17, 693, 728, 821, 898
HpySE526I ACGT 2 cut(s) 87, 159
Hsp92I GRCGYC 2 cut(s) 61, 87
Hsp92II CATG 4 cut(s) 251, 441, 830, 838
HspAI GCGC 1 cut(s) 556
Kzo9I GATC 2 cut(s) 307, 670
Lsp1109I GCAGC 2 cut(s) 121, 613
LweI GCATC 1 cut(s) 24
MaeI CTAG 2 cut(s) 296, 317
MaeII ACGT 2 cut(s) 87, 159
MaeIII GTNAC 1 cut(s) 403
MalI GATC 2 cut(s) 309, 672
MboI GATC 2 cut(s) 307, 670
MboII GAAGA 4 cut(s) 82, 221, 587, 842
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 9 cut(s) 238, 408, 530, 572, 654, 738, 777, 796, 812
MlyI GAGTC 2 cut(s) 554, 832
Mph1103I ATGCAT 1 cut(s) 913
MseI TTAA 4 cut(s) 95, 105, 186, 780
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 820
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 427
MvaI CCWGG 1 cut(s) 427
MvnI CGCG 1 cut(s) 558
MwoI GCNNNNNNNGC 1 cut(s) 340
NcoI CCATGG 1 cut(s) 247
NdeII GATC 2 cut(s) 307, 670
NlaIII CATG 4 cut(s) 251, 441, 830, 838
NmeAIII GCCGAG 1 cut(s) 30
NsiI ATGCAT 1 cut(s) 913
PagI TCATGA 1 cut(s) 826
PceI AGGCCT 1 cut(s) 266
PfeI GAWTC 1 cut(s) 760
PflMI CCANNNNNTGG 2 cut(s) 234, 891
PkrI GCNGC 3 cut(s) 136, 628, 631
PleI GAGTC 2 cut(s) 554, 831
PpsI GAGTC 2 cut(s) 554, 831
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 425
PspGI CCWGG 1 cut(s) 425
PstI CTGCAG 1 cut(s) 523
PsuI RGATCY 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 820
RsaI GTAC 2 cut(s) 701, 871
RsaNI GTAC 2 cut(s) 700, 870
RseI CAYNNNNRTG 1 cut(s) 252
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 4 cut(s) 95, 105, 186, 780
SatI GCNGC 3 cut(s) 135, 627, 630
Sau3AI GATC 2 cut(s) 307, 670
SchI GAGTC 2 cut(s) 554, 832
ScrFI CCNGG 1 cut(s) 427
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 1 cut(s) 24
SfcI CTRYAG 2 cut(s) 519, 852
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 9 cut(s) 238, 408, 530, 572, 654, 738, 777, 796, 812
SseBI AGGCCT 1 cut(s) 266
SsiI CCGC 1 cut(s) 629
SspMI CTAG 2 cut(s) 296, 317
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 425
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 4 cut(s) 10, 131, 450, 590
TaiI ACGT 2 cut(s) 90, 162
TasI AATT 9 cut(s) 238, 408, 530, 572, 654, 738, 777, 796, 812
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 1 cut(s) 760
Tru1I TTAA 4 cut(s) 95, 105, 186, 780
Tru9I TTAA 4 cut(s) 95, 105, 186, 780
TscAI CASTG 3 cut(s) 28, 528, 762
TseI GCWGC 2 cut(s) 134, 626
TspDTI ATGAA 2 cut(s) 208, 843
TspGWI ACGGA 1 cut(s) 717
TspRI CASTG 3 cut(s) 28, 528, 762
Van91I CCANNNNNTGG 2 cut(s) 234, 891
XagI CCTNNNNNAGG 1 cut(s) 618
XapI RAATTY 5 cut(s) 238, 572, 738, 796, 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmiI GTMKAC 1 cut(s) 877
XspI CTAG 2 cut(s) 296, 317
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.