AT2G23640

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005464
Physical Location & Seq
Forward (+)
10057542 .. 10059108
1567 bp
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UTR
Exon/CDS
Intron
AT2G23640.1

Sequence Viewer

Length: 621 bp
ATGGCCAACGACGTGACCAAAGATCCAACACCCAAATCTGACATTGTGGAGGATATTTACTTATGGAGGAGGAAGAAACTGGCCTTCTCTACACTCCTGGTCTCAACATCGACATGGATTTTACTTAGCTTTTATGGTTTCACTACCATAACCATTGTATCTTGGATCGGCATCGCAGTCGTCTCTATGATTTTCCTCTGGGGGAGTTTACTTCGCCTTCTCAGCAAGGTCGAACCGGAACTATCAGGATTAGAAGTATCGGAGGAGTTTGTAGTGGAGACAGTGAGGTCTTGTCGGATGCTAATGGAAGAAATGGTTAGATGGATGTTTCGAGTCGGTGCTGAGAGTGAATGGTTTGTTTTCGCGAGAACCGTTTTAGGTTTTTGGATCTTGTCAAGAATTGGGAACCTTCTCGATTTCCATACTTGTCTCTTCATCGGTTTGGTGATGGGGTTGACGGTTCCAAAGTTGTGGGAAGAGTACGGAGATCAAATACAGAAGCATTTGGGTAGCCTTAAAGATAAATCGAAGGGAGCGTATAATACAACTCATGAGAAGATTTTGGAAATGAAGAATAAGTTACACCATGGTACAGAAGAGAAAGTGAAAAAATCAGAGTAG

Protein Analysis

206

Amino Acids

23.77

Weight (kDa)

6.98

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 16 - 170 3.7e-38 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 286
AccII CGCG 1 cut(s) 365
AclWI GGATC 3 cut(s) 17, 173, 395
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 2 cut(s) 482, 592
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 1 cut(s) 129
AluI AGCT 1 cut(s) 129
Alw26I GTCTC 4 cut(s) 106, 187, 272, 434
AlwI GGATC 3 cut(s) 17, 173, 395
AoxI GGCC 2 cut(s) 3, 81
AsuHPI GGTGA 1 cut(s) 457
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
BalI TGGCCA 1 cut(s) 5
BccI CCATC 2 cut(s) 315, 442
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 4 cut(s) 106, 187, 272, 434
Bme1390I CCNGG 1 cut(s) 98
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 2 cut(s) 407, 462
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 2 cut(s) 180, 288
BsaBI GATNNNNATC 1 cut(s) 170
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 586
BsaWI WCCGGW 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 170
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 586
BseGI GGATG 2 cut(s) 303, 330
BseJI GATNNNNATC 1 cut(s) 170
BseMII CTCAG 2 cut(s) 235, 333
BseNI ACTGG 1 cut(s) 84
BseRI GAGGAG 2 cut(s) 82, 278
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 2 cut(s) 5, 83
BsiSI CCGG 1 cut(s) 236
BsmAI GTCTC 4 cut(s) 106, 187, 272, 434
BsmBI CGTCTC 1 cut(s) 187
BsnI GGCC 2 cut(s) 5, 83
Bso31I GGTCTC 1 cut(s) 106
Bsp143I GATC 4 cut(s) 22, 165, 387, 487
Bsp19I CCATGG 1 cut(s) 586
Bsp68I TCGCGA 1 cut(s) 365
BspANI GGCC 2 cut(s) 5, 83
BspCNI CTCAG 2 cut(s) 234, 334
BspFNI CGCG 1 cut(s) 365
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 407, 462
BspPI GGATC 3 cut(s) 17, 173, 395
BspTNI GGTCTC 1 cut(s) 106
BsrI ACTGG 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 586
BssMI GATC 4 cut(s) 22, 165, 387, 487
BssT1I CCWWGG 1 cut(s) 586
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 283, 373, 460
Bst6I CTCTTC 3 cut(s) 437, 471, 591
BstDEI CTNAG 3 cut(s) 125, 221, 342
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 2 cut(s) 303, 330
BstFNI CGCG 1 cut(s) 365
BstKTI GATC 4 cut(s) 25, 168, 390, 490
BstMAI GTCTC 4 cut(s) 106, 187, 272, 434
BstMBI GATC 4 cut(s) 22, 165, 387, 487
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 1 cut(s) 365
BstX2I RGATCY 2 cut(s) 22, 387
BstXI CCANNNNNNTGG 1 cut(s) 471
BstYI RGATCY 2 cut(s) 22, 387
BsuRI GGCC 2 cut(s) 5, 83
BtgI CCRYGG 1 cut(s) 586
BtgZI GCGATG 1 cut(s) 157
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 2 cut(s) 303, 330
BtsIMutI CAGTG 1 cut(s) 288
BtuMI TCGCGA 1 cut(s) 365
CciI TCATGA 1 cut(s) 550
Csp6I GTAC 2 cut(s) 481, 591
CviAII CATG 3 cut(s) 114, 551, 587
CviJI RGCY 4 cut(s) 5, 83, 129, 513
CviKI_1 RGCY 4 cut(s) 5, 83, 129, 513
CviQI GTAC 2 cut(s) 481, 591
DdeI CTNAG 3 cut(s) 125, 221, 342
DpnI GATC 4 cut(s) 24, 167, 389, 489
DpnII GATC 4 cut(s) 22, 165, 387, 487
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 437, 471, 591
EarI CTCTTC 3 cut(s) 437, 471, 591
Eco130I CCWWGG 1 cut(s) 586
Eco31I GGTCTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 586
ErhI CCWWGG 1 cut(s) 586
Esp3I CGTCTC 1 cut(s) 187
FaeI CATG 3 cut(s) 117, 554, 590
FaiI YATR 9 cut(s) 64, 115, 135, 149, 188, 423, 540, 552, 588
FatI CATG 3 cut(s) 113, 550, 586
FokI GGATG 2 cut(s) 310, 337
HaeIII GGCC 2 cut(s) 5, 83
HapII CCGG 1 cut(s) 236
Hin1II CATG 3 cut(s) 117, 554, 590
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HinfI GANTC 1 cut(s) 333
HpaII CCGG 1 cut(s) 236
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 2 cut(s) 209, 456
Hpy188I TCNGA 4 cut(s) 40, 262, 297, 616
Hpy188III TCNNGA 5 cut(s) 246, 364, 396, 413, 551
Hpy8I GTNNAC 2 cut(s) 209, 456
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 4 cut(s) 94, 227, 419, 523
HpyCH4III ACNGT 3 cut(s) 283, 373, 460
HpyCH4IV ACGT 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 3 cut(s) 125, 221, 342
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 3 cut(s) 117, 554, 590
Kzo9I GATC 4 cut(s) 22, 165, 387, 487
LmnI GCTCC 1 cut(s) 533
LpnPI CCDG 6 cut(s) 65, 83, 110, 184, 231, 249
LweI GCATC 2 cut(s) 180, 288
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 2 cut(s) 13, 579
MalI GATC 4 cut(s) 24, 167, 389, 489
MboI GATC 4 cut(s) 22, 165, 387, 487
MboII GAAGA 7 cut(s) 85, 320, 424, 488, 568, 583, 608
MflI RGATCY 2 cut(s) 22, 387
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 399
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 342
MmeI TCCRAC 2 cut(s) 50, 275
MnlI CCTC 6 cut(s) 43, 60, 63, 206, 256, 279
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 1 cut(s) 112
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 222
NcoI CCATGG 1 cut(s) 586
NdeII GATC 4 cut(s) 22, 165, 387, 487
NlaIII CATG 3 cut(s) 117, 554, 590
NlaIV GGNNCC 2 cut(s) 407, 462
NmuCI GTSAC 1 cut(s) 13
NruI TCGCGA 1 cut(s) 365
PagI TCATGA 1 cut(s) 550
PcsI WCGNNNNNNNCGW 2 cut(s) 369, 533
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 407, 462
PsuI RGATCY 2 cut(s) 22, 387
RruI TCGCGA 1 cut(s) 365
RsaI GTAC 2 cut(s) 482, 592
RsaNI GTAC 2 cut(s) 481, 591
RseI CAYNNNNRTG 1 cut(s) 112
SaqAI TTAA 1 cut(s) 516
Sau3AI GATC 4 cut(s) 22, 165, 387, 487
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 1 cut(s) 98
SetI ASST 6 cut(s) 15, 131, 231, 290, 382, 411
SfaNI GCATC 2 cut(s) 180, 288
SmiMI CAYNNNNRTG 1 cut(s) 112
Sse9I AATT 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 3 cut(s) 283, 373, 460
TaiI ACGT 1 cut(s) 15
TaqI TCGA 5 cut(s) 110, 231, 331, 414, 527
TasI AATT 1 cut(s) 399
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 424, 584
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.