Rroxscaffold_1G00062260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005464
Physical Location & Seq
Forward (+)
84310785 .. 84314093
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062260.1

Sequence Viewer

Length: 399 bp
ATGGCGGCGGCGAGGCTCGGGTTGGTGGATGGGTTGTCGGCTCCGTGTGCAATCGGATCGATATCGATCCGCTACCCCGGTTCATGGAGGTTTTGCGGCGGTGGTGCAGCGACGGATCTTGGGGCTCCGGCGGTGATCTCTTTCGCCACCATCCCCGCGTGTTGGGCCGTGGGTCGTCCCGATCTAGATGCGATGGTGCCGATCCCGGAAGGGACCCGTCCGATCTCGTTTTTGGATCCGGGGGGTTGTCGCCCTAGTGCCGGGGTGGTTTGTTGTGGCGTGTCAAGGCGGGGCTGCGGCGAAGGGTTTCGGCGTGATGCCGTTTTGACGCGTGACGATGGCCTCGGTAGCGTTGGCGTTGCTAGCGGTCGCGGATCCGCCGGTTGTGCTTGGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

13.18

Weight (kDa)

8.18

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AccII CGCG 3 cut(s) 158, 331, 372
AclWI GGATC 8 cut(s) 61, 64, 123, 196, 230, 243, 369, 382
AcoI YGGCCR 1 cut(s) 392
AfiI CCNNNNNNNGG 3 cut(s) 84, 162, 260
AflIII ACRYGT 1 cut(s) 329
AlwI GGATC 8 cut(s) 61, 64, 123, 196, 230, 243, 369, 382
Ama87I CYCGRG 1 cut(s) 17
AoxI GGCC 3 cut(s) 165, 340, 392
ApeKI GCWGC 2 cut(s) 107, 294
Asp700I GAANNNNTTC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 165, 213
AsuC2I CCSGG 4 cut(s) 78, 206, 240, 262
AsuHPI GGTGA 1 cut(s) 145
AsuNHI GCTAGC 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 17
AvaII GGWCC 1 cut(s) 213
BamHI GGATCC 2 cut(s) 235, 374
BanI GGYRCC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 127
BbvI GCAGC 2 cut(s) 119, 281
BccI CCATC 4 cut(s) 23, 158, 187, 332
BceAI ACGGC 3 cut(s) 152, 305, 379
BcgI CGANNNNNNTGC 4 cut(s) 39, 73, 170, 204
BcnI CCSGG 4 cut(s) 78, 206, 240, 262
BfaI CTAG 3 cut(s) 185, 255, 363
BisI GCNGC 6 cut(s) 6, 9, 97, 108, 295, 298
BlsI GCNGC 6 cut(s) 7, 10, 98, 109, 296, 299
Bme1390I CCNGG 4 cut(s) 78, 206, 240, 262
Bme18I GGWCC 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 165, 213
BmiI GGNNCC 7 cut(s) 42, 126, 198, 214, 215, 237, 376
BmrFI CCNGG 4 cut(s) 78, 206, 240, 262
BmsI GCATC 2 cut(s) 178, 307
BmtI GCTAGC 1 cut(s) 366
BpuMI CCSGG 4 cut(s) 78, 206, 240, 262
Bsa29I ATCGAT 2 cut(s) 59, 65
BsaBI GATNNNNATC 2 cut(s) 61, 65
BsaJI CCNNGG 5 cut(s) 76, 168, 239, 261, 343
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 162, 260
Bse118I RCCGGY 1 cut(s) 380
Bse8I GATNNNNATC 2 cut(s) 61, 65
BseCI ATCGAT 2 cut(s) 59, 65
BseDI CCNNGG 5 cut(s) 76, 168, 239, 261, 343
BseGI GGATG 2 cut(s) 34, 150
BseJI GATNNNNATC 2 cut(s) 61, 65
BseLI CCNNNNNNNGG 3 cut(s) 84, 162, 260
BseXI GCAGC 2 cut(s) 119, 281
BsgI GTGCAG 1 cut(s) 126
Bsh1236I CGCG 3 cut(s) 158, 331, 372
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 167, 342, 394
BshNI GGYRCC 1 cut(s) 196
BshVI ATCGAT 2 cut(s) 59, 65
BsiEI CGRYCG 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 17
BsiSI CCGG 6 cut(s) 78, 128, 206, 239, 261, 381
BslFI GGGAC 2 cut(s) 162, 226
BslI CCNNNNNNNGG 3 cut(s) 84, 162, 260
BsmFI GGGAC 2 cut(s) 162, 226
BsnI GGCC 3 cut(s) 167, 342, 394
BsoBI CYCGRG 1 cut(s) 17
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
BspANI GGCC 3 cut(s) 167, 342, 394
BspDI ATCGAT 2 cut(s) 59, 65
BspFNI CGCG 3 cut(s) 158, 331, 372
BspLI GGNNCC 7 cut(s) 42, 126, 198, 214, 215, 237, 376
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 8 cut(s) 61, 64, 123, 196, 230, 243, 369, 382
BspT107I GGYRCC 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 380
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 5 cut(s) 76, 168, 239, 261, 343
BssMI GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
BstC8I GCNNGC 1 cut(s) 364
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 34, 150
BstFNI CGCG 3 cut(s) 158, 331, 372
BstKTI GATC 9 cut(s) 59, 69, 118, 138, 184, 204, 225, 238, 377
BstMBI GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 5 cut(s) 47, 164, 348, 363, 386
BstSCI CCNGG 4 cut(s) 76, 204, 238, 260
BstUI CGCG 3 cut(s) 158, 331, 372
BstV1I GCAGC 2 cut(s) 119, 281
BstX2I RGATCY 3 cut(s) 115, 235, 374
BstYI RGATCY 3 cut(s) 115, 235, 374
Bsu15I ATCGAT 2 cut(s) 59, 65
BsuRI GGCC 3 cut(s) 167, 342, 394
BsuTUI ATCGAT 2 cut(s) 59, 65
BtgI CCRYGG 1 cut(s) 168
BtgZI GCGATG 1 cut(s) 206
BtsCI GGATG 2 cut(s) 34, 150
Cac8I GCNNGC 1 cut(s) 364
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 2 cut(s) 165, 213
ClaI ATCGAT 2 cut(s) 59, 65
CseI GACGC 1 cut(s) 337
CviAII CATG 1 cut(s) 84
CviJI RGCY 7 cut(s) 16, 41, 125, 167, 294, 342, 394
CviKI_1 RGCY 7 cut(s) 16, 41, 125, 167, 294, 342, 394
DpnI GATC 9 cut(s) 58, 68, 117, 137, 183, 203, 224, 237, 376
DpnII GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
EaeI YGGCCR 1 cut(s) 392
EciI GGCGGA 1 cut(s) 367
Eco24I GRGCYC 1 cut(s) 127
Eco32I GATATC 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 213
EcoRV GATATC 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 127
FaeI CATG 1 cut(s) 87
FaiI YATR 1 cut(s) 85
FaqI GGGAC 2 cut(s) 162, 226
FatI CATG 1 cut(s) 83
FauI CCCGC 2 cut(s) 163, 282
Fnu4HI GCNGC 6 cut(s) 6, 9, 97, 108, 295, 298
FokI GGATG 2 cut(s) 41, 137
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 6 cut(s) 6, 9, 97, 108, 295, 298
FspBI CTAG 3 cut(s) 185, 255, 363
GluI GCNGC 6 cut(s) 6, 9, 97, 108, 295, 298
HaeIII GGCC 3 cut(s) 167, 342, 394
HapII CCGG 6 cut(s) 78, 128, 206, 239, 261, 381
HgaI GACGC 1 cut(s) 337
Hin1II CATG 1 cut(s) 87
HpaII CCGG 6 cut(s) 78, 128, 206, 239, 261, 381
HphI GGTGA 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 56, 222
Hpy188III TCNNGA 2 cut(s) 179, 185
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 2 cut(s) 203, 296
HpyCH4V TGCA 2 cut(s) 50, 107
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 164, 348, 363, 386
Hsp92II CATG 1 cut(s) 87
KflI GGGWCCC 1 cut(s) 213
Kzo9I GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
LmnI GCTCC 2 cut(s) 46, 130
LpnPI CCDG 6 cut(s) 91, 141, 219, 252, 274, 394
Lsp1109I GCAGC 2 cut(s) 119, 281
LweI GCATC 2 cut(s) 178, 307
MaeI CTAG 3 cut(s) 185, 255, 363
MaeIII GTNAC 1 cut(s) 332
MalI GATC 9 cut(s) 58, 68, 117, 137, 183, 203, 224, 237, 376
MboI GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
MflI RGATCY 3 cut(s) 115, 235, 374
MhlI GDGCHC 1 cut(s) 127
MluI ACGCGT 1 cut(s) 329
MnlI CCTC 3 cut(s) 6, 81, 353
MroXI GAANNNNTTC 1 cut(s) 306
MspI CCGG 6 cut(s) 78, 128, 206, 239, 261, 381
MspR9I CCNGG 4 cut(s) 78, 206, 240, 262
MvnI CGCG 3 cut(s) 158, 331, 372
MwoI GCNNNNNNNGC 5 cut(s) 47, 164, 348, 363, 386
NciI CCSGG 4 cut(s) 78, 206, 240, 262
NdeII GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 1 cut(s) 87
NlaIV GGNNCC 7 cut(s) 42, 126, 198, 214, 215, 237, 376
NmuCI GTSAC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 306
PfoI TCCNGGA 1 cut(s) 204
PkrI GCNGC 6 cut(s) 7, 10, 98, 109, 296, 299
PpuMI RGGWCCY 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 213
PspN4I GGNNCC 7 cut(s) 42, 126, 198, 214, 215, 237, 376
PspPI GGNCC 2 cut(s) 165, 213
PspPPI RGGWCCY 1 cut(s) 213
PsuI RGATCY 3 cut(s) 115, 235, 374
SatI GCNGC 6 cut(s) 6, 9, 97, 108, 295, 298
Sau3AI GATC 9 cut(s) 56, 66, 115, 135, 181, 201, 222, 235, 374
Sau96I GGNCC 2 cut(s) 165, 213
ScrFI CCNGG 4 cut(s) 78, 206, 240, 262
SduI GDGCHC 1 cut(s) 127
SetI ASST 1 cut(s) 92
SfaNI GCATC 2 cut(s) 178, 307
SinI GGWCC 1 cut(s) 213
SspMI CTAG 3 cut(s) 185, 255, 363
StyD4I CCNGG 4 cut(s) 76, 204, 238, 260
TaqI TCGA 2 cut(s) 59, 65
TauI GCSGC 4 cut(s) 8, 11, 99, 300
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 107, 294
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 72
TspGWI ACGGA 2 cut(s) 33, 128
VpaK11BI GGWCC 1 cut(s) 213
XbaI TCTAGA 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 3 cut(s) 185, 255, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.