MD02G1163700.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005464
Physical Location & Seq
Forward (+)
14006611 .. 14007999
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1163700.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCTGCTACATCTCAATCTTCAGACTCTTCACTACCCAAGTTAGATACTGTGAGAGATATACTCCTGTGGAGGAAGAAGAAGCAGAGTTTTCTGGTTCTTTTGACAGCAACAGCGACATGGATTTTGTTGGACGTCTATCAATTCAACTTCCTCACTGTAATTTCATACGTTGCCATGTTTCTTGTCACTTCGTTGTTCCTTTCGGGCAACTTGCTTAGGCTTCTCGGCAAAGAGCCTCCAGATTTGTCAAGAGTTGGAATTACAGAGAAGTTCGCTTTGGAGATGGGAAACACCATTCGTGCTTGGGTTGAAGAAGGAATTCGATGGATGTTTCAGGTGGCTGCGGAGAGAGAGTGGTTTGTATTTGTTGGAACTGTCTCTGGTTTGTGGTTGCTCTCCCGTGTAGCAAGATGCGTTGATCTGCTTACTCTTCTTTATATAGGTATCGTTATGGGCATGACAGTTCCAGCAATGTATGTGAAATACGAGGACAAGATAGGGAGGAGTGAGGAGAAGTTGAAGGCACAGTTGAAAAGGTACTATGACACGCTTGATGAGAAGGTAGTCAAGAAAATACAGAACAAGGTGACAGTCGGGGAAAAGAAGGTGAAGAAGGTTGAGTAG

Protein Analysis

209

Amino Acids

23.83

Weight (kDa)

9.5

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 18 - 169 1.4e-40 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AciI CCGC 1 cut(s) 347
AcsI RAATTY 1 cut(s) 321
AcuI CTGAAG 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 542
AgsI TTSAA 4 cut(s) 148, 314, 523, 535
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
Alw26I GTCTC 1 cut(s) 385
ApeKI GCWGC 2 cut(s) 5, 344
ApoI RAATTY 1 cut(s) 321
ArsI GACNNNNNNTTYG 2 cut(s) 109, 141
Asp700I GAANNNNTTC 1 cut(s) 321
AsuHPI GGTGA 2 cut(s) 601, 622
BbvI GCAGC 1 cut(s) 331
BccI CCATC 2 cut(s) 280, 321
BcoDI GTCTC 1 cut(s) 385
BisI GCNGC 2 cut(s) 6, 345
BlsI GCNGC 2 cut(s) 7, 346
BmsI GCATC 1 cut(s) 404
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpmI CTGGAG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 135
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bse3DI GCAATG 1 cut(s) 480
BseGI GGATG 1 cut(s) 336
BseMI GCAATG 1 cut(s) 480
BseRI GAGGAG 2 cut(s) 520, 527
BseXI GCAGC 1 cut(s) 331
BsmAI GTCTC 1 cut(s) 385
Bsp143I GATC 1 cut(s) 421
BspACI CCGC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 480
BssMI GATC 1 cut(s) 421
BssNI GRCGYC 1 cut(s) 135
Bst4CI ACNGT 6 cut(s) 52, 160, 379, 466, 531, 595
Bst6I CTCTTC 2 cut(s) 34, 438
BstACI GRCGYC 1 cut(s) 135
BstDEI CTNAG 1 cut(s) 218
BstF5I GGATG 1 cut(s) 336
BstKTI GATC 1 cut(s) 424
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 156
Csp6I GTAC 1 cut(s) 541
CviAII CATG 3 cut(s) 120, 178, 460
CviJI RGCY 4 cut(s) 5, 223, 238, 344
CviKI_1 RGCY 4 cut(s) 5, 223, 238, 344
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 1 cut(s) 218
DpnI GATC 1 cut(s) 423
DpnII GATC 1 cut(s) 421
Eam1104I CTCTTC 2 cut(s) 34, 438
EarI CTCTTC 2 cut(s) 34, 438
Eco57I CTGAAG 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 321
FaeI CATG 3 cut(s) 123, 181, 463
FatI CATG 3 cut(s) 119, 177, 459
Fnu4HI GCNGC 2 cut(s) 6, 345
FokI GGATG 1 cut(s) 343
Fsp4HI GCNGC 2 cut(s) 6, 345
GluI GCNGC 2 cut(s) 6, 345
GsuI CTGGAG 1 cut(s) 225
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 3 cut(s) 123, 181, 463
HinfI GANTC 1 cut(s) 26
HphI GGTGA 2 cut(s) 601, 622
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 3 cut(s) 242, 252, 571
HpyAV CCTTC 5 cut(s) 311, 517, 556, 601, 610
HpyCH4III ACNGT 6 cut(s) 52, 160, 379, 466, 531, 595
HpyCH4IV ACGT 2 cut(s) 135, 171
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 2 cut(s) 135, 171
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 3 cut(s) 123, 181, 463
Kzo9I GATC 1 cut(s) 421
LpnPI CCDG 6 cut(s) 80, 80, 255, 323, 369, 483
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 404
MaeII ACGT 2 cut(s) 135, 171
MaeIII GTNAC 2 cut(s) 187, 589
MalI GATC 1 cut(s) 423
MboI GATC 1 cut(s) 421
MboII GAAGA 7 cut(s) 12, 21, 88, 91, 326, 425, 625
MluCI AATT 4 cut(s) 143, 162, 261, 321
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 3 cut(s) 111, 238, 352
MnlI CCTC 6 cut(s) 66, 164, 249, 484, 498, 505
MroXI GAANNNNTTC 1 cut(s) 321
NdeII GATC 1 cut(s) 421
NlaIII CATG 3 cut(s) 123, 181, 463
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 2 cut(s) 187, 589
PdmI GAANNNNTTC 1 cut(s) 321
PkrI GCNGC 2 cut(s) 7, 346
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
SatI GCNGC 2 cut(s) 6, 345
Sau3AI GATC 1 cut(s) 421
SchI GAGTC 1 cut(s) 20
SetI ASST 9 cut(s) 138, 174, 342, 448, 542, 567, 591, 612, 621
SfaNI GCATC 1 cut(s) 404
Sse9I AATT 4 cut(s) 143, 162, 261, 321
SsiI CCGC 1 cut(s) 347
TaaI ACNGT 6 cut(s) 52, 160, 379, 466, 531, 595
TaiI ACGT 2 cut(s) 138, 174
TaqI TCGA 1 cut(s) 325
TasI AATT 4 cut(s) 143, 162, 261, 321
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 2 cut(s) 187, 589
TseI GCWGC 2 cut(s) 5, 344
Tsp45I GTSAC 2 cut(s) 187, 589
TspDTI ATGAA 1 cut(s) 156
TspRI CASTG 1 cut(s) 163
XapI RAATTY 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 321
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.