Rmu_sc0003428.1_g000005

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003428.1
OG0005464
Physical Location & Seq
Forward (+)
30331 .. 30786
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003428.1_g000005.1.cds

Sequence Viewer

Length: 378 bp
atggcagcggcaaggctctgggttggtggatgggttgtcggctccgggtgtagtcggatcgatcttgatctgctaccccggttcatggaggttttgcggcggtggtgcagcgacggatcttggggctctggcggtgctctctttctccaccatccctgcgtgttgggctgtagctgtcctgatttagatgcgatggtgctgatcccggaagggacatggcggggctgcggcgaagggtttcagcgtgatgctgttttaacgcgtgacgatggcctggtggcgttggcgttgctggcggtcggtggatctgctggttgtgcttggccgtggctgggagtggtggtctttgggctgagcctatttcttaggcctctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.32

Weight (kDa)

5.5

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 262
AciI CCGC 7 cut(s) 8, 97, 100, 132, 220, 228, 296
AclWI GGATC 4 cut(s) 65, 124, 196, 313
AcoI YGGCCR 1 cut(s) 323
AfiI CCNNNNNNNGG 2 cut(s) 85, 332
AflIII ACRYGT 1 cut(s) 260
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 1 cut(s) 174
AluI AGCT 1 cut(s) 174
Alw21I GWGCWC 1 cut(s) 139
AlwI GGATC 4 cut(s) 65, 124, 196, 313
AoxI GGCC 3 cut(s) 271, 323, 368
ApeKI GCWGC 3 cut(s) 5, 108, 225
Asp700I GAANNNNTTC 1 cut(s) 237
AsuC2I CCSGG 3 cut(s) 46, 79, 206
BanII GRGCYC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 3 cut(s) 17, 120, 212
BccI CCATC 4 cut(s) 24, 159, 187, 263
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 275
BcnI CCSGG 3 cut(s) 46, 79, 206
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 6 cut(s) 6, 9, 98, 109, 226, 229
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 6 cut(s) 7, 10, 99, 110, 227, 230
Bme1390I CCNGG 4 cut(s) 46, 79, 206, 275
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 4 cut(s) 46, 79, 206, 275
BmsI GCATC 2 cut(s) 178, 238
Bpu1102I GCTNAGC 1 cut(s) 353
BpuMI CCSGG 3 cut(s) 46, 79, 206
Bsa29I ATCGAT 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 77, 326
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 332
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 1 cut(s) 275
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 2 cut(s) 77, 326
BseGI GGATG 2 cut(s) 35, 151
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 85, 332
BseMII CTCAG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 17, 120, 212
BseYI CCCAGC 1 cut(s) 331
BsgI GTGCAG 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 262
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 273, 325, 370
BshVI ATCGAT 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 3 cut(s) 45, 79, 206
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 2 cut(s) 85, 332
BsmFI GGGAC 1 cut(s) 226
BsnI GGCC 3 cut(s) 273, 325, 370
Bsp1286I GDGCHC 2 cut(s) 128, 139
Bsp143I GATC 6 cut(s) 57, 61, 67, 116, 201, 305
Bsp1720I GCTNAGC 1 cut(s) 353
BspACI CCGC 7 cut(s) 8, 97, 100, 132, 220, 228, 296
BspANI GGCC 3 cut(s) 273, 325, 370
BspCNI CTCAG 1 cut(s) 345
BspDI ATCGAT 1 cut(s) 60
BspFNI CGCG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 43
BspPI GGATC 4 cut(s) 65, 124, 196, 313
BssECI CCNNGG 2 cut(s) 77, 326
BssMI GATC 6 cut(s) 57, 61, 67, 116, 201, 305
Bst2UI CCWGG 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 353, 365
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 35, 151
BstFNI CGCG 1 cut(s) 262
BstKTI GATC 6 cut(s) 60, 64, 70, 119, 204, 308
BstMBI GATC 6 cut(s) 57, 61, 67, 116, 201, 305
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 3 cut(s) 165, 293, 317
BstNI CCWGG 1 cut(s) 275
BstSCI CCNGG 4 cut(s) 44, 77, 204, 273
BstSFI CTRYAG 1 cut(s) 169
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 3 cut(s) 17, 120, 212
BstX2I RGATCY 2 cut(s) 116, 305
BstYI RGATCY 2 cut(s) 116, 305
Bsu15I ATCGAT 1 cut(s) 60
BsuRI GGCC 3 cut(s) 273, 325, 370
BsuTUI ATCGAT 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 326
BtgZI GCGATG 1 cut(s) 206
BtsCI GGATG 2 cut(s) 35, 151
Cac8I GCNNGC 1 cut(s) 294
ClaI ATCGAT 1 cut(s) 60
CviAII CATG 2 cut(s) 85, 216
DdeI CTNAG 2 cut(s) 353, 365
DpnI GATC 6 cut(s) 59, 63, 69, 118, 203, 307
DpnII GATC 6 cut(s) 57, 61, 67, 116, 201, 305
EaeI YGGCCR 1 cut(s) 323
Eco147I AGGCCT 1 cut(s) 370
Eco24I GRGCYC 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 128
FaeI CATG 2 cut(s) 88, 219
FaiI YATR 2 cut(s) 86, 217
FaqI GGGAC 1 cut(s) 226
FatI CATG 2 cut(s) 84, 215
FauI CCCGC 1 cut(s) 213
Fnu4HI GCNGC 6 cut(s) 6, 9, 98, 109, 226, 229
FokI GGATG 2 cut(s) 42, 138
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 6 cut(s) 6, 9, 98, 109, 226, 229
GluI GCNGC 6 cut(s) 6, 9, 98, 109, 226, 229
GsaI CCCAGC 1 cut(s) 335
HaeIII GGCC 3 cut(s) 273, 325, 370
HapII CCGG 3 cut(s) 45, 79, 206
Hin1II CATG 2 cut(s) 88, 219
HpaII CCGG 3 cut(s) 45, 79, 206
Hpy188I TCNGA 2 cut(s) 57, 377
Hpy188III TCNNGA 2 cut(s) 65, 179
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 203, 227
HpyCH4V TGCA 1 cut(s) 108
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 293, 317
HpyF3I CTNAG 2 cut(s) 353, 365
Hsp92II CATG 2 cut(s) 88, 219
Kzo9I GATC 6 cut(s) 57, 61, 67, 116, 201, 305
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 3 cut(s) 17, 120, 212
LweI GCATC 2 cut(s) 178, 238
MaeIII GTNAC 1 cut(s) 263
MalI GATC 6 cut(s) 59, 63, 69, 118, 203, 307
MboI GATC 6 cut(s) 57, 61, 67, 116, 201, 305
MflI RGATCY 2 cut(s) 116, 305
MhlI GDGCHC 2 cut(s) 128, 139
MluI ACGCGT 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 35
MnlI CCTC 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 3 cut(s) 45, 79, 206
MspR9I CCNGG 4 cut(s) 46, 79, 206, 275
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 1 cut(s) 262
MwoI GCNNNNNNNGC 3 cut(s) 165, 293, 317
NciI CCSGG 3 cut(s) 46, 79, 206
NdeII GATC 6 cut(s) 57, 61, 67, 116, 201, 305
NlaIII CATG 2 cut(s) 88, 219
NlaIV GGNNCC 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 263
PceI AGGCCT 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 237
PfoI TCCNGGA 1 cut(s) 204
PkrI GCNGC 6 cut(s) 7, 10, 99, 110, 227, 230
Psp6I CCWGG 1 cut(s) 273
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 43
PsuI RGATCY 2 cut(s) 116, 305
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 6 cut(s) 6, 9, 98, 109, 226, 229
Sau3AI GATC 6 cut(s) 57, 61, 67, 116, 201, 305
ScrFI CCNGG 4 cut(s) 46, 79, 206, 275
SduI GDGCHC 2 cut(s) 128, 139
SetI ASST 2 cut(s) 93, 176
SfaNI GCATC 2 cut(s) 178, 238
SfcI CTRYAG 1 cut(s) 169
SseBI AGGCCT 1 cut(s) 370
SsiI CCGC 7 cut(s) 8, 97, 100, 132, 220, 228, 296
StuI AGGCCT 1 cut(s) 370
StyD4I CCNGG 4 cut(s) 44, 77, 204, 273
TaqI TCGA 1 cut(s) 60
TauI GCSGC 3 cut(s) 11, 100, 231
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 3 cut(s) 5, 108, 225
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 1 cut(s) 73
TspGWI ACGGA 1 cut(s) 129
XmnI GAANNNNTTC 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.