Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001675

Physical Location & Seq

Genomic Coordinates Forward (+)

12824372 .. 12826684

Gene Structure 2313 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G30050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 909 bp

ATGCCAGGTCAGAAGATTGAAACGGGTCATGAGGACATTGTCCATGATGTGCAGATGGATTACTATGGAAAGCGAATTGCAACTGCATCATCTGACTGCACCATCAAGATAACCGGTGTCAGCAACAACGGTGGATCGCAGCAACTAGCCACATTAACCGGCCACCGTGGTCCTGTCTGGGAAGTCGCGTGGGCACACCCAAAGTATGGATCAATCCTTGCTTCATGCTCCTATGATGGTCAAGTCATACTCTGGAAAGAAGGCAACCAGAACCAATGGACCCAAGATCATGTTTTCACAGACCACAAATCTTCAGTCAACTCCATTGCATGGGCTCCTCATGATATCGGACTATCCTTGGCCTGCGGGTCATCTGATGGCAACATTTCGGTTTTCACAGCCCGTGCTGACGGTGGCTGGGACACATCAAGGATTGACCAAGCACATCCGGTTGGAGTCACTTCAGTCTCATGGGCCCCAGCCACAGCGCCTGGTGCTCTTGTGAGCTCTGGTCTTCTCGATCCGGTTTACAAGCTAGCTTCTGGTGGATGTGATAATACCGTGAAAGTGTGGAAGCTCGCTAACGGGTCATGGAAGATGGATTGCTTTCCAGCTCTTCAAAAGCACACTGATTGGGTCCGTGATGTGGCTTGGGCACCAAACTTGGGTCTCCCCAAGTCCACCATAGCCAGTGGTTCCCAAGATGGGAAAGTTATCATATGGACAGTGGGAAAAGAAGGTGAGCAATGGGAAGGTAAGGTTCTCAAGGACTTTATGACTCCTGTGTGGCGGGTCTCGTGGTCGTTGACCGGTAACTTATTGGCCGTATCCGATGGGAACAACAACGTGACTGTGTGGAAAGAGGCTGTTGATGGAGAGTGGGAACAAGTTACTGCTGTTGAGCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005829 GO:0006810 GO:0006888 GO:0008150 GO:0012505 GO:0016020 GO:0016192 GO:0031080 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0098588 GO:0098805 GO:0098852

KEGG Pathways

Metabolic & Signaling

ko03013 ko04141 ko04150 map03013 map04141 map04150

Pfam Domains

Protein Families

WD40

Protein Analysis

302

Amino Acids

32.64

Weight (kDa)

5.42

Isoelectric Point (pI)

25.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_TEP1_2nd	PF25047	7 - 132	1.3e-06	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	8 - 161	1.5e-15	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	8 - 91	2.6e-10	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	10 - 91	2.5e-12	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	14 - 111	1.7e-10	WDHD1 first WD40 domain
WD40_Prp19	PF24814	17 - 137	4.1e-11	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	19 - 160	1.7e-10	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	24 - 159	2.3e-07	G protein beta WD-40 repeat protein
WD40	PF00400	47 - 85	5.1e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	49 - 135	5.5e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	92 - 243	1.7e-11	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	120 - 244	4.4e-09	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	174 - 248	6.3e-09	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	178 - 288	1e-07	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	178 - 287	2.8e-12	WDR5 beta-propeller domain
WD40_Prp19	PF24814	178 - 283	3.9e-07	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	178 - 287	2.8e-14	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	205 - 287	1e-05	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001675)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30050 AT3G01340 AT3G01340
fragaria_vesca	FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g49840 FvH4_6g49840 FvH4_6g49840
malus_domestica	MD09G1041000.v1.1 MD09G1205500.v1.1 MD17G1042300.v1.1 MD17G1185700.v1.1
prunus_persica	Prupe.3G060800_v2.0.a1 Prupe.3G276700_v2.0.a1
pyrus_communis	pycom09g12210 pycom111g03200 pycom17g03790 pycom17g19510
rosa_chinensis	RchiOBHm_Chr2g0140101 RchiOBHm_Chr2g0170031
rosa_laevigata	RLG00000010169 RLG00000019849 RLG00000021917
rosa_multiflora	Rmu_co8506925.1_g000001 Rmu_sc0003244.1_g000028 Rmu_sc0013078.1_g000015 Rmu_ssc0000389.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081680 Rroxscaffold_2G00105060
rosa_rugosa	Rorug02G0361100 Rorug02G0361200 Rorug02G0545000 Rorug02G0545100
rosa_samantha	Rh2AG411800 Rh2AG617500 Rh2BG422100 Rh2BG628800 Rh2CG397900 Rh2CG598300 Rh2DG431500 Rh2DG640000 Rh2DG640600 Rh4BG007800
rosa_wichuraiana	Rw2G033740 Rw2G051120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	655
AccB7I	CCANNNNNTGG	2 cut(s)	206, 330
AccII	CGCG	1 cut(s)	188
AciI	CCGC	2 cut(s)	366, 790
AclWI	GGATC	3 cut(s)	142, 217, 515
AcoI	YGGCCR	2 cut(s)	160, 822
AcuI	CTGAAG	2 cut(s)	297, 447
AfiI	CCNNNNNNNGG	5 cut(s)	206, 330, 646, 665, 705
AgeI	ACCGGT	2 cut(s)	113, 809
AgsI	TTSAA	2 cut(s)	20, 620
AjnI	CCWGG	2 cut(s)	4, 490
AluBI	AGCT	5 cut(s)	507, 535, 539, 577, 614
AluI	AGCT	5 cut(s)	507, 535, 539, 577, 614
Alw21I	GWGCWC	2 cut(s)	499, 509
Alw26I	GTCTC	3 cut(s)	472, 674, 799
AlwI	GGATC	3 cut(s)	142, 217, 515
AlwNI	CAGNNNCTG	1 cut(s)	491
AoxI	GGCC	4 cut(s)	160, 360, 474, 822
ApaI	GGGCCC	1 cut(s)	478
ApeKI	GCWGC	1 cut(s)	139
ArsI	GACNNNNNNTTYG	2 cut(s)	300, 332
AsiGI	ACCGGT	2 cut(s)	113, 809
AspLEI	GCGC	1 cut(s)	490
AspS9I	GGNCC	5 cut(s)	170, 279, 474, 475, 637
AsuHPI	GGTGA	1 cut(s)	752
AsuNHI	GCTAGC	1 cut(s)	535
AvaII	GGWCC	3 cut(s)	170, 279, 637
BaeGI	GKGCMC	3 cut(s)	196, 478, 658
BanI	GGYRCC	1 cut(s)	655
BanII	GRGCYC	3 cut(s)	337, 478, 509
BauI	CACGAG	1 cut(s)	796
BbsI	GAAGAC	1 cut(s)	506
Bbv12I	GWGCWC	2 cut(s)	499, 509
BbvI	GCAGC	1 cut(s)	151
BccI	CCATC	8 cut(s)	49, 110, 230, 371, 592, 698, 827, 866
BceAI	ACGGC	1 cut(s)	809
BciT130I	CCWGG	2 cut(s)	6, 492
BciVI	GTATCC	1 cut(s)	838
BcoDI	GTCTC	3 cut(s)	472, 674, 799
BfaI	CTAG	2 cut(s)	146, 536
BfoI	RGCGCY	1 cut(s)	491
BfuI	GTATCC	1 cut(s)	838
BisI	GCNGC	1 cut(s)	140
BlsI	GCNGC	1 cut(s)	141
Bme1390I	CCNGG	2 cut(s)	6, 492
Bme18I	GGWCC	3 cut(s)	170, 279, 637
BmgT120I	GGNCC	5 cut(s)	170, 279, 474, 475, 637
BmiI	GGNNCC	7 cut(s)	281, 336, 476, 477, 638, 657, 697
BmrFI	CCNGG	2 cut(s)	6, 492
BmsI	GCATC	1 cut(s)	95
BmtI	GCTAGC	1 cut(s)	539
BpiI	GAAGAC	1 cut(s)	506
BpuEI	CTTGAG	1 cut(s)	749
BsaI	GGTCTC	2 cut(s)	674, 799
BsaJI	CCNNGG	2 cut(s)	166, 357
BsaWI	WCCGGW	4 cut(s)	113, 448, 523, 809
Bsc4I	CCNNNNNNNGG	5 cut(s)	206, 330, 646, 665, 705
Bse118I	RCCGGY	3 cut(s)	113, 158, 809
Bse1I	ACTGG	1 cut(s)	690
Bse3DI	GCAATG	2 cut(s)	324, 752
BseBI	CCWGG	2 cut(s)	6, 492
BseDI	CCNNGG	2 cut(s)	166, 357
BseGI	GGATG	2 cut(s)	445, 554
BseLI	CCNNNNNNNGG	5 cut(s)	206, 330, 646, 665, 705
BseMI	GCAATG	2 cut(s)	324, 752
BseNI	ACTGG	1 cut(s)	690
BseRI	GAGGAG	1 cut(s)	327
BseSI	GKGCMC	3 cut(s)	196, 478, 658
BseXI	GCAGC	1 cut(s)	151
BseYI	CCCAGC	2 cut(s)	417, 478
BsgI	GTGCAG	2 cut(s)	71, 82
Bsh1236I	CGCG	1 cut(s)	188
BshFI	GGCC	4 cut(s)	162, 362, 476, 824
BshNI	GGYRCC	1 cut(s)	655
BshTI	ACCGGT	2 cut(s)	113, 809
BsiHKAI	GWGCWC	2 cut(s)	499, 509
BsiSI	CCGG	5 cut(s)	114, 159, 449, 524, 810
BslFI	GGGAC	1 cut(s)	434
BslI	CCNNNNNNNGG	5 cut(s)	206, 330, 646, 665, 705
BsmAI	GTCTC	3 cut(s)	472, 674, 799
BsmFI	GGGAC	1 cut(s)	434
BsnI	GGCC	4 cut(s)	162, 362, 476, 824
Bso31I	GGTCTC	2 cut(s)	674, 799
Bsp120I	GGGCCC	1 cut(s)	474
Bsp1286I	GDGCHC	6 cut(s)	196, 337, 478, 499, 509, 658
Bsp143I	GATC	4 cut(s)	134, 209, 286, 520
BspACI	CCGC	2 cut(s)	366, 790
BspANI	GGCC	4 cut(s)	162, 362, 476, 824
BspFNI	CGCG	1 cut(s)	188
BspHI	TCATGA	2 cut(s)	28, 340
BspLI	GGNNCC	7 cut(s)	281, 336, 476, 477, 638, 657, 697
BspOI	GCTAGC	1 cut(s)	539
BspPI	GGATC	3 cut(s)	142, 217, 515
BspQI	GCTCTTC	1 cut(s)	621
BspT107I	GGYRCC	1 cut(s)	655
BspTNI	GGTCTC	2 cut(s)	674, 799
BsrDI	GCAATG	2 cut(s)	324, 752
BsrFI	RCCGGY	3 cut(s)	113, 158, 809
BsrI	ACTGG	1 cut(s)	690
BssAI	RCCGGY	3 cut(s)	113, 158, 809
BssECI	CCNNGG	2 cut(s)	166, 357
BssMI	GATC	4 cut(s)	134, 209, 286, 520
BssSI	CACGAG	1 cut(s)	796
BssT1I	CCWWGG	1 cut(s)	357
Bst2BI	CACGAG	1 cut(s)	796
Bst2UI	CCWGG	2 cut(s)	6, 492
Bst4CI	ACNGT	6 cut(s)	131, 167, 413, 562, 727, 853
Bst6I	CTCTTC	1 cut(s)	621
BstC8I	GCNNGC	3 cut(s)	364, 537, 579
BstDEI	CTNAG	1 cut(s)	906
BstDSI	CCRYGG	1 cut(s)	166
BstF5I	GGATG	2 cut(s)	445, 554
BstFNI	CGCG	1 cut(s)	188
BstH2I	RGCGCY	1 cut(s)	491
BstHHI	GCGC	1 cut(s)	490
BstKTI	GATC	4 cut(s)	137, 212, 289, 523
BstMAI	GTCTC	3 cut(s)	472, 674, 799
BstMBI	GATC	4 cut(s)	134, 209, 286, 520
BstMWI	GCNNNNNNNGC	1 cut(s)	494
BstNI	CCWGG	2 cut(s)	6, 492
BstSCI	CCNGG	2 cut(s)	4, 490
BstSLI	GKGCMC	3 cut(s)	196, 478, 658
BstUI	CGCG	1 cut(s)	188
BstV1I	GCAGC	1 cut(s)	151
BstV2I	GAAGAC	1 cut(s)	506
BsuI	GTATCC	1 cut(s)	838
BsuRI	GGCC	4 cut(s)	162, 362, 476, 824
BtgI	CCRYGG	1 cut(s)	166
BtsCI	GGATG	2 cut(s)	445, 554
BtsIMutI	CAGTG	3 cut(s)	627, 697, 732
Cac8I	GCNNGC	3 cut(s)	364, 537, 579
CaiI	CAGNNNCTG	1 cut(s)	491
CciI	TCATGA	2 cut(s)	28, 340
CfoI	GCGC	1 cut(s)	490
Cfr10I	RCCGGY	3 cut(s)	113, 158, 809
Cfr13I	GGNCC	5 cut(s)	170, 279, 474, 475, 637
CspAI	ACCGGT	2 cut(s)	113, 809
CspCI	CAANNNNNGTGG	2 cut(s)	112, 147
CviAII	CATG	8 cut(s)	29, 44, 225, 290, 330, 341, 471, 591
DdeI	CTNAG	1 cut(s)	906
DpnI	GATC	4 cut(s)	136, 211, 288, 522
DpnII	GATC	4 cut(s)	134, 209, 286, 520
EaeI	YGGCCR	2 cut(s)	160, 822
Eam1104I	CTCTTC	1 cut(s)	621
EarI	CTCTTC	1 cut(s)	621
Ecl136II	GAGCTC	1 cut(s)	507
Eco130I	CCWWGG	1 cut(s)	357
Eco24I	GRGCYC	3 cut(s)	337, 478, 509
Eco31I	GGTCTC	2 cut(s)	674, 799
Eco32I	GATATC	1 cut(s)	346
Eco47I	GGWCC	3 cut(s)	170, 279, 637
Eco53kI	GAGCTC	1 cut(s)	507
Eco57I	CTGAAG	2 cut(s)	297, 447
EcoICRI	GAGCTC	1 cut(s)	507
EcoO109I	RGGNCCY	1 cut(s)	475
EcoRII	CCWGG	2 cut(s)	4, 490
EcoRV	GATATC	1 cut(s)	346
EcoT14I	CCWWGG	1 cut(s)	357
EcoT38I	GRGCYC	3 cut(s)	337, 478, 509
ErhI	CCWWGG	1 cut(s)	357
FaeI	CATG	8 cut(s)	32, 47, 228, 293, 333, 344, 474, 594
FaqI	GGGAC	1 cut(s)	434
FatI	CATG	8 cut(s)	28, 43, 224, 289, 329, 340, 470, 590
FauI	CCCGC	2 cut(s)	359, 783
FauNDI	CATATG	1 cut(s)	719
Fnu4HI	GCNGC	1 cut(s)	140
FokI	GGATG	2 cut(s)	432, 561
FriOI	GRGCYC	3 cut(s)	337, 478, 509
Fsp4HI	GCNGC	1 cut(s)	140
FspBI	CTAG	2 cut(s)	146, 536
GlaI	GCGC	1 cut(s)	489
GluI	GCNGC	1 cut(s)	140
GsaI	CCCAGC	2 cut(s)	421, 482
HaeII	RGCGCY	1 cut(s)	491
HaeIII	GGCC	4 cut(s)	162, 362, 476, 824
HapII	CCGG	5 cut(s)	114, 159, 449, 524, 810
HhaI	GCGC	1 cut(s)	490
Hin1II	CATG	8 cut(s)	32, 47, 228, 293, 333, 344, 474, 594
Hin6I	GCGC	1 cut(s)	488
HinP1I	GCGC	1 cut(s)	488
HincII	GTYRAC	2 cut(s)	319, 807
HindII	GTYRAC	2 cut(s)	319, 807
HinfI	GANTC	2 cut(s)	456, 778
HpaII	CCGG	5 cut(s)	114, 159, 449, 524, 810
HphI	GGTGA	1 cut(s)	752
Hpy166II	GTNNAC	4 cut(s)	319, 529, 681, 807
Hpy188I	TCNGA	5 cut(s)	12, 94, 350, 376, 832
Hpy188III	TCNNGA	5 cut(s)	29, 106, 253, 341, 518
Hpy8I	GTNNAC	4 cut(s)	319, 529, 681, 807
HpyAV	CCTTC	3 cut(s)	254, 731, 746
HpyCH4III	ACNGT	6 cut(s)	131, 167, 413, 562, 727, 853
HpyCH4IV	ACGT	1 cut(s)	846
HpyCH4V	TGCA	5 cut(s)	52, 80, 86, 99, 329
HpyF10VI	GCNNNNNNNGC	1 cut(s)	494
HpyF3I	CTNAG	1 cut(s)	906
HpySE526I	ACGT	1 cut(s)	846
Hsp92II	CATG	8 cut(s)	32, 47, 228, 293, 333, 344, 474, 594
HspAI	GCGC	1 cut(s)	488
Kzo9I	GATC	4 cut(s)	134, 209, 286, 520
LguI	GCTCTTC	1 cut(s)	621
LmnI	GCTCC	2 cut(s)	233, 340
Lsp1109I	GCAGC	1 cut(s)	151
LweI	GCATC	1 cut(s)	95
MaeI	CTAG	2 cut(s)	146, 536
MaeII	ACGT	1 cut(s)	846
MaeIII	GTNAC	4 cut(s)	457, 812, 847, 889
MalI	GATC	4 cut(s)	136, 211, 288, 522
MboI	GATC	4 cut(s)	134, 209, 286, 520
MboII	GAAGA	5 cut(s)	25, 303, 506, 607, 608
MhlI	GDGCHC	6 cut(s)	196, 337, 478, 499, 509, 658
MluCI	AATT	1 cut(s)	75
MlyI	GAGTC	2 cut(s)	465, 772
MmeI	TCCRAC	1 cut(s)	433
MnlI	CCTC	3 cut(s)	25, 348, 856
MseI	TTAA	1 cut(s)	155
MspI	CCGG	5 cut(s)	114, 159, 449, 524, 810
MspR9I	CCNGG	2 cut(s)	6, 492
MvaI	CCWGG	2 cut(s)	6, 492
MvnI	CGCG	1 cut(s)	188
MwoI	GCNNNNNNNGC	1 cut(s)	494
NdeI	CATATG	1 cut(s)	719
NdeII	GATC	4 cut(s)	134, 209, 286, 520
NheI	GCTAGC	1 cut(s)	535
NlaIII	CATG	8 cut(s)	32, 47, 228, 293, 333, 344, 474, 594
NlaIV	GGNNCC	7 cut(s)	281, 336, 476, 477, 638, 657, 697
NmuCI	GTSAC	2 cut(s)	457, 847
PagI	TCATGA	2 cut(s)	28, 340
PciSI	GCTCTTC	1 cut(s)	621
PflFI	GACNNNGTC	1 cut(s)	38
PflMI	CCANNNNNTGG	2 cut(s)	206, 330
PinAI	ACCGGT	2 cut(s)	113, 809
PkrI	GCNGC	1 cut(s)	141
PleI	GAGTC	2 cut(s)	464, 772
PpsI	GAGTC	2 cut(s)	464, 772
Psp124BI	GAGCTC	1 cut(s)	509
Psp6I	CCWGG	2 cut(s)	4, 490
PspFI	CCCAGC	2 cut(s)	417, 478
PspGI	CCWGG	2 cut(s)	4, 490
PspN4I	GGNNCC	7 cut(s)	281, 336, 476, 477, 638, 657, 697
PspOMI	GGGCCC	1 cut(s)	474
PspPI	GGNCC	5 cut(s)	170, 279, 474, 475, 637
PstNI	CAGNNNCTG	1 cut(s)	491
PsyI	GACNNNGTC	1 cut(s)	38
SacI	GAGCTC	1 cut(s)	509
SapI	GCTCTTC	1 cut(s)	621
SaqAI	TTAA	1 cut(s)	155
SatI	GCNGC	1 cut(s)	140
Sau3AI	GATC	4 cut(s)	134, 209, 286, 520
Sau96I	GGNCC	5 cut(s)	170, 279, 474, 475, 637
SchI	GAGTC	2 cut(s)	465, 772
ScrFI	CCNGG	2 cut(s)	6, 492
SduI	GDGCHC	6 cut(s)	196, 337, 478, 499, 509, 658
SfaNI	GCATC	1 cut(s)	95
SinI	GGWCC	3 cut(s)	170, 279, 637
SmlI	CTYRAG	1 cut(s)	764
SmoI	CTYRAG	1 cut(s)	764
Sse9I	AATT	1 cut(s)	75
SsiI	CCGC	2 cut(s)	366, 790
SspMI	CTAG	2 cut(s)	146, 536
SstI	GAGCTC	1 cut(s)	509
StyD4I	CCNGG	2 cut(s)	4, 490
StyI	CCWWGG	1 cut(s)	357
TaaI	ACNGT	6 cut(s)	131, 167, 413, 562, 727, 853
TaiI	ACGT	1 cut(s)	849
TaqI	TCGA	1 cut(s)	519
TasI	AATT	1 cut(s)	75
Tru1I	TTAA	1 cut(s)	155
Tru9I	TTAA	1 cut(s)	155
TscAI	CASTG	3 cut(s)	634, 697, 732
TseFI	GTSAC	2 cut(s)	457, 847
TseI	GCWGC	1 cut(s)	139
Tsp45I	GTSAC	2 cut(s)	457, 847
TspDTI	ATGAA	1 cut(s)	213
TspGWI	ACGGA	1 cut(s)	629
TspRI	CASTG	3 cut(s)	634, 697, 732
Tth111I	GACNNNGTC	1 cut(s)	38
Van91I	CCANNNNNTGG	2 cut(s)	206, 330
VpaK11BI	GGWCC	3 cut(s)	170, 279, 637
XspI	CTAG	2 cut(s)	146, 536

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.