Rroxscaffold_2G00105060

Belongs to the WD repeat SEC13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001675
Physical Location & Seq
Forward (+)
28496281 .. 28499384
3104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105060.1

Sequence Viewer

Length: 951 bp
ATGCCTGCGCAGAAGGTTGAGACTGGTCACCAGGACACTGTCCACGATGTGGTCATGGATTACTATGGCAAGCGCCTGGCCACAGCTTCGTCGGACAACACCATCAAGATAGTGGGGGTGAGCAACACGGCCTCGCAACATCTTGCGACCCTGACTGGTCACCAGGGACCTGTTTGGCAGGTTGCTTGGGCTCACCTGAAATTCGGGTCTTTACTTGCTTCTTGTTCTTATGATGGGCGTGTCATTCTGTGGAAGGAAGGCAATCAGAACGAGTGGACACAAGCCCATGTTTTTGATGACCACAAATCATCTGTCAACTCAATTGCTTGGGCTCCTCATGAACTTGGTCTCTGTTTGGCTTGTGGTTCTTCCGATGGGAATATCTCGGTTTTCACTGCTAAAGCAGACGGTGGTTGGGAGACCACAAAGATCGACCAAGCTCACCCAGTTGGTGTAACCTCTGTTTCCTGGGCTCCCTCAACAGCTCCTGGTGCTCTTGTTGGCTCTGGTCTGCTTGACACTGTTCAGAAGCTGTGTTCTGGTGGTTGTGATAACACTGTAAAGGTCTGGAAGCTTACTAATGGCGTTTGGAAAATGGACTGTTTTCCAGCTCTTCAAATGCATGTTGATTGGGTTAGGGATGTTGCTTGGGCACCTAACTTGGGACTACCAAAATCTACTATTGCCAGTGCATCACAGGATGGGAAAGTTATTATCTGGACTGTGGCCAAGGATGGGGATCAGTGGGAAGGTAAGGTGCTGCATGATTTCAAGGCACCTGTTTGGAGGGTCTCATGGTCACTTACCGGGAACATATTGGCCGTGGCTGATGGGAACAACAGCGTGACACTGTGGAAGGAAGAAGTTGATGGGGAGTGGCAACAGGTGACCACAATACCTAATGCGGAATATGGACTTCAAGAACAGCCGAGAGACCGCATATATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0002376 GO:0002474 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006996 GO:0008104 GO:0008150 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019882 GO:0019884 GO:0019886 GO:0022607 GO:0023051 GO:0023056 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031080 GO:0031090 GO:0031410 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031982 GO:0031984 GO:0032006 GO:0032008 GO:0032991 GO:0033036 GO:0034613 GO:0034622 GO:0035459 GO:0035859 GO:0042175 GO:0042802 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048002 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048475 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051668 GO:0061024 GO:0061700 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098827 GO:0098852 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko03013 ko04141 ko04150 map03013 map04141 map04150
Pfam Domains
Protein Families
WD40

Protein Analysis

316

Amino Acids

34.48

Weight (kDa)

5.61

Isoelectric Point (pI)

26.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 4 - 146 3.8e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 8 - 159 8.3e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 89 2e-10 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 9 - 136 8.1e-06 MABP1/WDR62 second WD40 domain
WDR55 PF24796 9 - 286 8e-07 WDR55
WD40_CDC20-Fz PF24807 9 - 160 2.7e-13 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 9 - 112 1.5e-11 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 10 - 91 2.1e-13 THOC3 beta-propeller domain
WD40_Prp19 PF24814 23 - 172 2.5e-12 Prp19 WD40 domain
WD40_Gbeta PF25391 25 - 160 6.9e-06 G protein beta WD-40 repeat protein
WD40 PF00400 46 - 84 5.4e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 49 - 135 1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 93 - 286 4.8e-11 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 173 - 246 8.9e-07 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 176 - 289 1.2e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 176 - 287 2.8e-11 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 176 - 286 3.8e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 9
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 2 cut(s) 652, 775
AccB7I CCANNNNNTGG 1 cut(s) 49
AciI CCGC 2 cut(s) 905, 937
AclWI GGATC 1 cut(s) 747
AcoI YGGCCR 3 cut(s) 78, 726, 819
AcsI RAATTY 1 cut(s) 200
AdeI CACNNNGTG 1 cut(s) 49
AfiI CCNNNNNNNGG 3 cut(s) 49, 662, 735
AgsI TTSAA 3 cut(s) 617, 772, 920
AjnI CCWGG 5 cut(s) 30, 75, 162, 467, 487
AluBI AGCT 6 cut(s) 86, 440, 485, 532, 574, 611
AluI AGCT 6 cut(s) 86, 440, 485, 532, 574, 611
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 5 cut(s) 14, 353, 413, 796, 927
AlwI GGATC 1 cut(s) 747
AlwNI CAGNNNCTG 2 cut(s) 488, 532
AoxI GGCC 4 cut(s) 78, 129, 726, 819
ApeKI GCWGC 1 cut(s) 760
ApoI RAATTY 1 cut(s) 200
ArsI GACNNNNNNTTYG 2 cut(s) 297, 329
AspLEI GCGC 2 cut(s) 10, 75
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 808
AsuHPI GGTGA 6 cut(s) 20, 130, 152, 185, 434, 898
AvaII GGWCC 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 655
BalI TGGCCA 2 cut(s) 80, 728
BanI GGYRCC 2 cut(s) 652, 775
BanII GRGCYC 3 cut(s) 193, 334, 475
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 1 cut(s) 747
BccI CCATC 7 cut(s) 110, 227, 368, 695, 728, 824, 863
BceAI ACGGC 2 cut(s) 144, 806
BciT130I CCWGG 5 cut(s) 32, 77, 164, 469, 489
BcnI CCSGG 1 cut(s) 808
BcoDI GTCTC 5 cut(s) 14, 353, 413, 796, 927
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 1 cut(s) 761
BlsI GCNGC 1 cut(s) 762
Bme1390I CCNGG 6 cut(s) 32, 77, 164, 469, 489, 808
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 5 cut(s) 168, 333, 474, 654, 777
BmrFI CCNGG 6 cut(s) 32, 77, 164, 469, 489, 808
BmrI ACTGGG 1 cut(s) 440
BmsI GCATC 1 cut(s) 701
BmuI ACTGGG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 808
BsaBI GATNNNNATC 1 cut(s) 738
BsaI GGTCTC 4 cut(s) 353, 413, 796, 927
BsaJI CCNNGG 4 cut(s) 163, 468, 729, 822
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 662, 735
Bse1I ACTGG 4 cut(s) 28, 160, 446, 687
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 5 cut(s) 32, 77, 164, 469, 489
BseDI CCNNGG 4 cut(s) 163, 468, 729, 822
BseGI GGATG 3 cut(s) 646, 706, 739
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 3 cut(s) 49, 662, 735
BseNI ACTGG 4 cut(s) 28, 160, 446, 687
BseRI GAGGAG 1 cut(s) 324
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 1 cut(s) 747
BshFI GGCC 4 cut(s) 80, 131, 728, 821
BshNI GGYRCC 2 cut(s) 652, 775
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 1 cut(s) 807
BslFI GGGAC 2 cut(s) 180, 678
BslI CCNNNNNNNGG 3 cut(s) 49, 662, 735
BsmAI GTCTC 5 cut(s) 14, 353, 413, 796, 927
BsmFI GGGAC 2 cut(s) 180, 678
BsnI GGCC 4 cut(s) 80, 131, 728, 821
Bso31I GGTCTC 4 cut(s) 353, 413, 796, 927
Bsp1286I GDGCHC 5 cut(s) 193, 334, 475, 496, 655
Bsp143I GATC 2 cut(s) 429, 739
BspACI CCGC 2 cut(s) 905, 937
BspANI GGCC 4 cut(s) 80, 131, 728, 821
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 5 cut(s) 168, 333, 474, 654, 777
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 1 cut(s) 747
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 2 cut(s) 652, 775
BspTNI GGTCTC 4 cut(s) 353, 413, 796, 927
BsrI ACTGG 4 cut(s) 28, 160, 446, 687
BssECI CCNNGG 4 cut(s) 163, 468, 729, 822
BssMI GATC 2 cut(s) 429, 739
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 5 cut(s) 32, 77, 164, 469, 489
Bst4CI ACNGT 7 cut(s) 40, 410, 523, 559, 602, 724, 852
Bst6I CTCTTC 1 cut(s) 618
BstC8I GCNNGC 2 cut(s) 6, 71
BstDSI CCRYGG 1 cut(s) 822
BstEII GGTNACC 3 cut(s) 26, 158, 886
BstF5I GGATG 3 cut(s) 646, 706, 739
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 10, 75
BstKTI GATC 2 cut(s) 432, 742
BstMAI GTCTC 5 cut(s) 14, 353, 413, 796, 927
BstMBI GATC 2 cut(s) 429, 739
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstNI CCWGG 5 cut(s) 32, 77, 164, 469, 489
BstNSI RCATGY 1 cut(s) 626
BstPI GGTNACC 3 cut(s) 26, 158, 886
BstSCI CCNGG 6 cut(s) 30, 75, 162, 467, 487, 806
BstSLI GKGCMC 1 cut(s) 655
BstV1I GCAGC 1 cut(s) 747
BsuRI GGCC 4 cut(s) 80, 131, 728, 821
BtgI CCRYGG 1 cut(s) 822
BtsCI GGATG 3 cut(s) 646, 706, 739
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 7 cut(s) 36, 393, 519, 555, 694, 749, 848
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 6, 71
CaiI CAGNNNCTG 2 cut(s) 488, 532
CciI TCATGA 1 cut(s) 337
CfoI GCGC 2 cut(s) 10, 75
Cfr13I GGNCC 1 cut(s) 167
CviAII CATG 6 cut(s) 55, 287, 338, 623, 764, 795
DpnI GATC 2 cut(s) 431, 741
DpnII GATC 2 cut(s) 429, 739
DraIII CACNNNGTG 1 cut(s) 49
EaeI YGGCCR 3 cut(s) 78, 726, 819
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 729
Eco24I GRGCYC 3 cut(s) 193, 334, 475
Eco31I GGTCTC 4 cut(s) 353, 413, 796, 927
Eco47I GGWCC 1 cut(s) 167
Eco91I GGTNACC 3 cut(s) 26, 158, 886
EcoO109I RGGNCCY 1 cut(s) 167
EcoO65I GGTNACC 3 cut(s) 26, 158, 886
EcoRII CCWGG 5 cut(s) 30, 75, 162, 467, 487
EcoT14I CCWWGG 1 cut(s) 729
EcoT22I ATGCAT 1 cut(s) 624
EcoT38I GRGCYC 3 cut(s) 193, 334, 475
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 6 cut(s) 58, 290, 341, 626, 767, 798
FaqI GGGAC 2 cut(s) 180, 678
FatI CATG 6 cut(s) 54, 286, 337, 622, 763, 794
Fnu4HI GCNGC 1 cut(s) 761
FokI GGATG 3 cut(s) 653, 713, 746
FriOI GRGCYC 3 cut(s) 193, 334, 475
Fsp4HI GCNGC 1 cut(s) 761
FspI TGCGCA 1 cut(s) 9
GlaI GCGC 2 cut(s) 9, 74
GluI GCNGC 1 cut(s) 761
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 4 cut(s) 80, 131, 728, 821
HapII CCGG 1 cut(s) 807
HhaI GCGC 2 cut(s) 10, 75
Hin1II CATG 6 cut(s) 58, 290, 341, 626, 767, 798
Hin6I GCGC 2 cut(s) 8, 73
HinP1I GCGC 2 cut(s) 8, 73
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 572
HpaII CCGG 1 cut(s) 807
HphI GGTGA 6 cut(s) 20, 130, 152, 185, 434, 898
Hpy166II GTNNAC 3 cut(s) 43, 276, 316
Hpy188I TCNGA 4 cut(s) 94, 267, 373, 528
Hpy188III TCNNGA 5 cut(s) 106, 338, 568, 718, 920
Hpy8I GTNNAC 3 cut(s) 43, 276, 316
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 5 cut(s) 7, 247, 251, 743, 850
HpyCH4III ACNGT 7 cut(s) 40, 410, 523, 559, 602, 724, 852
HpyCH4V TGCA 3 cut(s) 622, 692, 763
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
Hsp92II CATG 6 cut(s) 58, 290, 341, 626, 767, 798
HspAI GCGC 2 cut(s) 8, 73
Kzo9I GATC 2 cut(s) 429, 739
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 3 cut(s) 337, 478, 490
Lsp1109I GCAGC 1 cut(s) 747
LweI GCATC 1 cut(s) 701
MaeIII GTNAC 6 cut(s) 26, 158, 454, 798, 844, 886
MalI GATC 2 cut(s) 431, 741
MboI GATC 2 cut(s) 429, 739
MboII GAAGA 3 cut(s) 360, 605, 872
MfeI CAATTG 1 cut(s) 321
MhlI GDGCHC 5 cut(s) 193, 334, 475, 496, 655
MlsI TGGCCA 2 cut(s) 80, 728
MluCI AATT 2 cut(s) 200, 321
MluNI TGGCCA 2 cut(s) 80, 728
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 5 cut(s) 142, 345, 469, 487, 780
Mox20I TGGCCA 2 cut(s) 80, 728
Mph1103I ATGCAT 1 cut(s) 624
MscI TGGCCA 2 cut(s) 80, 728
Msp20I TGGCCA 2 cut(s) 80, 728
MspI CCGG 1 cut(s) 807
MspR9I CCNGG 6 cut(s) 32, 77, 164, 469, 489, 808
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 5 cut(s) 32, 77, 164, 469, 489
MwoI GCNNNNNNNGC 1 cut(s) 491
NciI CCSGG 1 cut(s) 808
NdeII GATC 2 cut(s) 429, 739
NlaIII CATG 6 cut(s) 58, 290, 341, 626, 767, 798
NlaIV GGNNCC 5 cut(s) 168, 333, 474, 654, 777
NmuCI GTSAC 5 cut(s) 26, 158, 798, 844, 886
NsbI TGCGCA 1 cut(s) 9
NsiI ATGCAT 1 cut(s) 624
NspI RCATGY 1 cut(s) 626
PagI TCATGA 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 618
PflFI GACNNNGTC 1 cut(s) 38
PflMI CCANNNNNTGG 1 cut(s) 49
PkrI GCNGC 1 cut(s) 762
PpuMI RGGWCCY 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 167
Psp6I CCWGG 5 cut(s) 30, 75, 162, 467, 487
PspEI GGTNACC 3 cut(s) 26, 158, 886
PspGI CCWGG 5 cut(s) 30, 75, 162, 467, 487
PspN4I GGNNCC 5 cut(s) 168, 333, 474, 654, 777
PspPI GGNCC 1 cut(s) 167
PspPPI RGGWCCY 1 cut(s) 167
PstNI CAGNNNCTG 2 cut(s) 488, 532
PsyI GACNNNGTC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 618
SatI GCNGC 1 cut(s) 761
Sau3AI GATC 2 cut(s) 429, 739
Sau96I GGNCC 1 cut(s) 167
ScrFI CCNGG 6 cut(s) 32, 77, 164, 469, 489, 808
SduI GDGCHC 5 cut(s) 193, 334, 475, 496, 655
SfaNI GCATC 1 cut(s) 701
SinI GGWCC 1 cut(s) 167
Sse9I AATT 2 cut(s) 200, 321
SsiI CCGC 2 cut(s) 905, 937
StyD4I CCNGG 6 cut(s) 30, 75, 162, 467, 487, 806
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 7 cut(s) 40, 410, 523, 559, 602, 724, 852
TaqI TCGA 1 cut(s) 432
TasI AATT 2 cut(s) 200, 321
TscAI CASTG 7 cut(s) 43, 400, 526, 562, 694, 749, 855
TseFI GTSAC 5 cut(s) 26, 158, 798, 844, 886
TseI GCWGC 1 cut(s) 760
Tsp45I GTSAC 5 cut(s) 26, 158, 798, 844, 886
TspDTI ATGAA 1 cut(s) 354
TspRI CASTG 7 cut(s) 43, 400, 526, 562, 694, 749, 855
Tth111I GACNNNGTC 1 cut(s) 38
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 1 cut(s) 200
XceI RCATGY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.