FvH4_6g32220

Belongs to the WD repeat SEC13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001675
Physical Location & Seq
Reverse (-)
25274910 .. 25278000
3091 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32220.t2

Sequence Viewer

Length: 942 bp
ATGCCTGCGCAGAAGGTTGAGACTGGTCACCAGGAGACTGTCCATGATGTGGTCATGGATTACTATGGCAAGCGCCTGGCCACTGCTTCGTCGGACAACACCATCAAGATAGTGGGAGTGAGCAACTCGGCCTCGCAGCATCTTGCGACGCTGACTGGTCACCAGGGCCCTGTTTGGCAGGTTGCTTGGGCTCACCCCAAATTCGGGTCTTTGCTTGCTTCTTGCTCCTATGACGGGCGCGTCATTTTGTGGAAGGAAGGGAATCAGAACGAGTGGACACAAGCTCATGTTTTCGATGACCACAAATCGTCTGTCAACTCAATTGCTTGGGCTCCTCATGAACTCGGTCTTTGTTTGGCTTGTGGTTCTTCTGATGGGACTATCTCAGTTTTCACTGCTAAAGACGGTGTTTGGGAGACCACAAAGATTGACGAAGCTCACCCGGTTGGTGTCACCTCTGTTTCCTGGGCTCCCTCAACAGCTCCTGGTGCTCTTGTTGGTTCTGGTCTGCTTGACACTGTTCAGAAGCTGTGTTCTGGTGGTTGTGATAACACTGTAAAGATCTGGAAGCTTACTAATGGTGCTTGGAAGATGGACTGTTTTCCAGCTCTTCAGAAGCATGATGATTGGGTTAGGGACGTTGCTTGGGCACCTAACTTGGGTCTACCAAAATCTACTGTTGCCAGTGCATCACAGGATGGTAAAGTTATCATCTGGACTGTGGCCAAGGATGGGGATAAATGGGAACATAAGGTGCTGCATGATTTCAAGGCACCTGTTTGGAGGGTCTCATGGTCACTTACTGGGAACATATTGGCTGTGTCTGATGGGAACAACAAGGTGACATTGTGGAAGGAAGAAGTTGATGGGGAGTGGCAACAGATACCTAACGCGGAATATGGACCTCAACCGCCGCCTGGTGTCCGTGGCTTAATATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0002376 GO:0002474 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006996 GO:0008104 GO:0008150 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019882 GO:0019884 GO:0019886 GO:0022607 GO:0023051 GO:0023056 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031080 GO:0031090 GO:0031410 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031982 GO:0031984 GO:0032006 GO:0032008 GO:0032991 GO:0033036 GO:0034613 GO:0034622 GO:0035459 GO:0035859 GO:0042175 GO:0042802 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048002 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048475 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051668 GO:0061024 GO:0061700 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098827 GO:0098852 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko03013 ko04141 ko04150 map03013 map04141 map04150
Pfam Domains
Protein Families
WD40

Protein Analysis

314

Amino Acids

34.06

Weight (kDa)

5.92

Isoelectric Point (pI)

24.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 4 - 190 2.9e-08 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 6 - 158 6e-14 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 8 - 135 3.2e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 8 - 90 9.9e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 159 3.1e-18 WDR5 beta-propeller domain
WDR55 PF24796 9 - 133 2.2e-06 WDR55
WD40_CDC20-Fz PF24807 9 - 159 2.3e-14 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 10 - 91 2e-14 THOC3 beta-propeller domain
WD40_Prp19 PF24814 22 - 172 4.8e-14 Prp19 WD40 domain
Beta-prop_WDR35_TULP_N PF24797 43 - 159 1.8e-07 WDR35/TULP4 N-terminal
WD40 PF00400 46 - 84 1.6e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 49 - 134 6.9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 69 - 157 5.6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 93 - 285 6.3e-12 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 119 - 243 9.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 172 - 246 4.5e-08 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 175 - 285 4e-07 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 175 - 287 6.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 175 - 287 6.6e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 175 - 285 3e-14 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
Acc16I TGCGCA 1 cut(s) 9
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 2 cut(s) 649, 772
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 1 cut(s) 664
AccII CGCG 2 cut(s) 240, 893
AciI CCGC 3 cut(s) 893, 911, 914
AcoI YGGCCR 2 cut(s) 78, 723
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 596
AfiI CCNNNNNNNGG 7 cut(s) 49, 203, 204, 234, 659, 732, 917
AgsI TTSAA 1 cut(s) 769
AjnI CCWGG 6 cut(s) 30, 75, 162, 464, 484, 916
AluBI AGCT 6 cut(s) 284, 437, 482, 529, 571, 608
AluI AGCT 6 cut(s) 284, 437, 482, 529, 571, 608
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 4 cut(s) 14, 29, 410, 793
AlwNI CAGNNNCTG 2 cut(s) 485, 529
AoxI GGCC 4 cut(s) 78, 129, 166, 723
ApaI GGGCCC 1 cut(s) 170
ApeKI GCWGC 2 cut(s) 136, 757
ApoI RAATTY 1 cut(s) 200
ArsI GACNNNNNNTTYG 2 cut(s) 297, 329
AspLEI GCGC 3 cut(s) 10, 75, 240
AspS9I GGNCC 3 cut(s) 166, 167, 902
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 6 cut(s) 20, 152, 185, 431, 445, 853
AvaII GGWCC 1 cut(s) 902
BaeGI GKGCMC 2 cut(s) 170, 652
BalI TGGCCA 2 cut(s) 80, 725
BanI GGYRCC 2 cut(s) 649, 772
BanII GRGCYC 4 cut(s) 170, 193, 334, 472
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 2 cut(s) 148, 744
BccI CCATC 7 cut(s) 110, 368, 586, 692, 725, 821, 860
BciT130I CCWGG 6 cut(s) 32, 77, 164, 466, 486, 918
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 4 cut(s) 14, 29, 410, 793
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 1 cut(s) 169
BglII AGATCT 1 cut(s) 561
BisI GCNGC 3 cut(s) 137, 758, 914
BlsI GCNGC 3 cut(s) 138, 759, 915
Bme1390I CCNGG 7 cut(s) 32, 77, 164, 443, 466, 486, 918
Bme18I GGWCC 1 cut(s) 902
BmgT120I GGNCC 3 cut(s) 166, 167, 902
BmiI GGNNCC 5 cut(s) 168, 333, 471, 651, 774
BmrFI CCNGG 7 cut(s) 32, 77, 164, 443, 466, 486, 918
BmrI ACTGGG 1 cut(s) 813
BmsI GCATC 2 cut(s) 148, 698
BmuI ACTGGG 1 cut(s) 813
BpuMI CCSGG 1 cut(s) 443
BsaI GGTCTC 2 cut(s) 410, 793
BsaJI CCNNGG 4 cut(s) 163, 465, 726, 925
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 203, 204, 234, 659, 732, 917
Bse1I ACTGG 4 cut(s) 28, 160, 684, 808
BseBI CCWGG 6 cut(s) 32, 77, 164, 466, 486, 918
BseDI CCNNGG 4 cut(s) 163, 465, 726, 925
BseGI GGATG 2 cut(s) 703, 736
BseLI CCNNNNNNNGG 7 cut(s) 49, 203, 204, 234, 659, 732, 917
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 4 cut(s) 28, 160, 684, 808
BseRI GAGGAG 1 cut(s) 324
BseSI GKGCMC 2 cut(s) 170, 652
BseXI GCAGC 2 cut(s) 148, 744
Bsh1236I CGCG 2 cut(s) 240, 893
BshFI GGCC 4 cut(s) 80, 131, 168, 725
BshNI GGYRCC 2 cut(s) 649, 772
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 1 cut(s) 443
BslFI GGGAC 2 cut(s) 391, 650
BslI CCNNNNNNNGG 7 cut(s) 49, 203, 204, 234, 659, 732, 917
BsmAI GTCTC 4 cut(s) 14, 29, 410, 793
BsmFI GGGAC 2 cut(s) 391, 650
BsnI GGCC 4 cut(s) 80, 131, 168, 725
Bso31I GGTCTC 2 cut(s) 410, 793
Bsp120I GGGCCC 1 cut(s) 166
Bsp1286I GDGCHC 6 cut(s) 170, 193, 334, 472, 493, 652
Bsp143I GATC 1 cut(s) 561
BspACI CCGC 3 cut(s) 893, 911, 914
BspANI GGCC 4 cut(s) 80, 131, 168, 725
BspCNI CTCAG 1 cut(s) 398
BspFNI CGCG 2 cut(s) 240, 893
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 5 cut(s) 168, 333, 471, 651, 774
BspMI ACCTGC 1 cut(s) 169
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 2 cut(s) 649, 772
BspTNI GGTCTC 2 cut(s) 410, 793
BsrI ACTGG 4 cut(s) 28, 160, 684, 808
BssECI CCNNGG 4 cut(s) 163, 465, 726, 925
BssMI GATC 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 726
Bst2UI CCWGG 6 cut(s) 32, 77, 164, 466, 486, 918
Bst4CI ACNGT 7 cut(s) 40, 407, 520, 556, 599, 679, 721
Bst6I CTCTTC 1 cut(s) 615
BstC8I GCNNGC 3 cut(s) 6, 71, 216
BstDEI CTNAG 1 cut(s) 385
BstDSI CCRYGG 1 cut(s) 925
BstEII GGTNACC 2 cut(s) 26, 158
BstF5I GGATG 2 cut(s) 703, 736
BstFNI CGCG 2 cut(s) 240, 893
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 3 cut(s) 10, 75, 240
BstKTI GATC 1 cut(s) 564
BstMAI GTCTC 4 cut(s) 14, 29, 410, 793
BstMBI GATC 1 cut(s) 561
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 6 cut(s) 32, 77, 164, 466, 486, 918
BstPI GGTNACC 2 cut(s) 26, 158
BstSCI CCNGG 7 cut(s) 30, 75, 162, 441, 464, 484, 916
BstSLI GKGCMC 2 cut(s) 170, 652
BstUI CGCG 2 cut(s) 240, 893
BstV1I GCAGC 2 cut(s) 148, 744
BstX2I RGATCY 1 cut(s) 561
BstYI RGATCY 1 cut(s) 561
BsuRI GGCC 4 cut(s) 80, 131, 168, 725
BtgI CCRYGG 1 cut(s) 925
BtsCI GGATG 2 cut(s) 703, 736
BtsI GCAGTG 2 cut(s) 81, 393
BtsIMutI CAGTG 5 cut(s) 81, 393, 516, 552, 691
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 3 cut(s) 6, 71, 216
CaiI CAGNNNCTG 2 cut(s) 485, 529
CciI TCATGA 1 cut(s) 337
CfoI GCGC 3 cut(s) 10, 75, 240
Cfr13I GGNCC 3 cut(s) 166, 167, 902
CseI GACGC 2 cut(s) 157, 229
CspCI CAANNNNNGTGG 2 cut(s) 409, 444
CviAII CATG 7 cut(s) 44, 55, 287, 338, 620, 761, 792
DdeI CTNAG 1 cut(s) 385
DpnI GATC 1 cut(s) 563
DpnII GATC 1 cut(s) 561
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 2 cut(s) 78, 723
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco130I CCWWGG 1 cut(s) 726
Eco24I GRGCYC 4 cut(s) 170, 193, 334, 472
Eco31I GGTCTC 2 cut(s) 410, 793
Eco47I GGWCC 1 cut(s) 902
Eco57I CTGAAG 1 cut(s) 596
Eco91I GGTNACC 2 cut(s) 26, 158
EcoO109I RGGNCCY 2 cut(s) 166, 167
EcoO65I GGTNACC 2 cut(s) 26, 158
EcoRII CCWGG 6 cut(s) 30, 75, 162, 464, 484, 916
EcoT14I CCWWGG 1 cut(s) 726
EcoT38I GRGCYC 4 cut(s) 170, 193, 334, 472
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 7 cut(s) 47, 58, 290, 341, 623, 764, 795
FaqI GGGAC 2 cut(s) 391, 650
FatI CATG 7 cut(s) 43, 54, 286, 337, 619, 760, 791
FblI GTMKAC 1 cut(s) 664
Fnu4HI GCNGC 3 cut(s) 137, 758, 914
FokI GGATG 2 cut(s) 710, 743
FriOI GRGCYC 4 cut(s) 170, 193, 334, 472
Fsp4HI GCNGC 3 cut(s) 137, 758, 914
FspI TGCGCA 1 cut(s) 9
GlaI GCGC 3 cut(s) 9, 74, 239
GluI GCNGC 3 cut(s) 137, 758, 914
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 4 cut(s) 80, 131, 168, 725
HapII CCGG 1 cut(s) 443
HgaI GACGC 2 cut(s) 157, 229
HhaI GCGC 3 cut(s) 10, 75, 240
Hin1II CATG 7 cut(s) 47, 58, 290, 341, 623, 764, 795
Hin6I GCGC 3 cut(s) 8, 73, 238
HinP1I GCGC 3 cut(s) 8, 73, 238
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 1 cut(s) 262
HpaII CCGG 1 cut(s) 443
HphI GGTGA 6 cut(s) 20, 152, 185, 431, 445, 853
Hpy166II GTNNAC 3 cut(s) 276, 316, 665
Hpy188I TCNGA 6 cut(s) 94, 267, 373, 525, 615, 826
Hpy188III TCNNGA 4 cut(s) 106, 338, 565, 715
Hpy8I GTNNAC 3 cut(s) 276, 316, 665
Hpy99I CGWCG 2 cut(s) 94, 151
HpyAV CCTTC 4 cut(s) 7, 247, 251, 847
HpyCH4III ACNGT 7 cut(s) 40, 407, 520, 556, 599, 679, 721
HpyCH4IV ACGT 1 cut(s) 639
HpyCH4V TGCA 2 cut(s) 689, 760
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 1 cut(s) 385
HpySE526I ACGT 1 cut(s) 639
Hsp92II CATG 7 cut(s) 47, 58, 290, 341, 623, 764, 795
HspAI GCGC 3 cut(s) 8, 73, 238
Kzo9I GATC 1 cut(s) 561
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 4 cut(s) 230, 337, 475, 487
Lsp1109I GCAGC 2 cut(s) 148, 744
LweI GCATC 2 cut(s) 148, 698
MaeII ACGT 1 cut(s) 639
MaeIII GTNAC 5 cut(s) 26, 158, 451, 795, 841
MalI GATC 1 cut(s) 563
MboI GATC 1 cut(s) 561
MboII GAAGA 4 cut(s) 360, 601, 602, 869
MfeI CAATTG 1 cut(s) 321
MflI RGATCY 1 cut(s) 561
MhlI GDGCHC 6 cut(s) 170, 193, 334, 472, 493, 652
MlsI TGGCCA 2 cut(s) 80, 725
MluCI AATT 2 cut(s) 200, 321
MluNI TGGCCA 2 cut(s) 80, 725
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 6 cut(s) 142, 345, 466, 484, 777, 915
Mox20I TGGCCA 2 cut(s) 80, 725
MscI TGGCCA 2 cut(s) 80, 725
MseI TTAA 1 cut(s) 932
Msp20I TGGCCA 2 cut(s) 80, 725
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 7 cut(s) 32, 77, 164, 443, 466, 486, 918
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 6 cut(s) 32, 77, 164, 466, 486, 918
MvnI CGCG 2 cut(s) 240, 893
MwoI GCNNNNNNNGC 1 cut(s) 488
NciI CCSGG 1 cut(s) 443
NdeII GATC 1 cut(s) 561
NlaIII CATG 7 cut(s) 47, 58, 290, 341, 623, 764, 795
NlaIV GGNNCC 5 cut(s) 168, 333, 471, 651, 774
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 5 cut(s) 26, 158, 451, 795, 841
NsbI TGCGCA 1 cut(s) 9
PagI TCATGA 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 262
PflMI CCANNNNNTGG 1 cut(s) 49
PkrI GCNGC 3 cut(s) 138, 759, 915
Psp6I CCWGG 6 cut(s) 30, 75, 162, 464, 484, 916
PspEI GGTNACC 2 cut(s) 26, 158
PspGI CCWGG 6 cut(s) 30, 75, 162, 464, 484, 916
PspN4I GGNNCC 5 cut(s) 168, 333, 471, 651, 774
PspOMI GGGCCC 1 cut(s) 166
PspPI GGNCC 3 cut(s) 166, 167, 902
PstNI CAGNNNCTG 2 cut(s) 485, 529
PsuI RGATCY 1 cut(s) 561
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 932
SatI GCNGC 3 cut(s) 137, 758, 914
Sau3AI GATC 1 cut(s) 561
Sau96I GGNCC 3 cut(s) 166, 167, 902
ScrFI CCNGG 7 cut(s) 32, 77, 164, 443, 466, 486, 918
SduI GDGCHC 6 cut(s) 170, 193, 334, 472, 493, 652
SfaNI GCATC 2 cut(s) 148, 698
SinI GGWCC 1 cut(s) 902
Sse9I AATT 2 cut(s) 200, 321
SsiI CCGC 3 cut(s) 893, 911, 914
StyD4I CCNGG 7 cut(s) 30, 75, 162, 441, 464, 484, 916
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 7 cut(s) 40, 407, 520, 556, 599, 679, 721
TaiI ACGT 1 cut(s) 642
TaqI TCGA 1 cut(s) 294
TaqII GACCGA 1 cut(s) 335
TasI AATT 2 cut(s) 200, 321
TauI GCSGC 1 cut(s) 916
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 1 cut(s) 932
Tru9I TTAA 1 cut(s) 932
TscAI CASTG 5 cut(s) 88, 400, 523, 559, 691
TseFI GTSAC 5 cut(s) 26, 158, 451, 795, 841
TseI GCWGC 2 cut(s) 136, 757
Tsp45I GTSAC 5 cut(s) 26, 158, 451, 795, 841
TspDTI ATGAA 1 cut(s) 354
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 5 cut(s) 88, 400, 523, 559, 691
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 902
XapI RAATTY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.