Rorug02G0361200

Belongs to the WD repeat SEC13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001675
Physical Location & Seq
Reverse (-)
45737678 .. 45740350
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0361200.1

Sequence Viewer

Length: 978 bp
ATGGACTACTCATCAGCTGCTGTGTATGATACTTCTCTGGATCTTAACACCAGGCCTCTCCGGCTTTTCGATGATTCTCCTCCGATCAAGAAAGAGGTGCAGAGCAAGATTTTGTTCGACTTTGGAAGGGAGCTCTCAGCAAAAGAAGAGAGTGGAGCTTTGGTAGAGGAATTGCACAGGGTGAGTACCGAAAACAAGAAGCTGACTGAAATGTTGACGGTGATGGGTGAGAGCTACAATGCTTTGAGAAGCCAATTGTTGGAGTACATGAGCAAGAACCCAGAGAAGGAGCTCAGCCCGATATCCAAGAAAAGAAAGTCTGAAAGTAGTAACAACAACAACAACAACAACAACAGTGCTAACAACATCCTTAATGGCATCAACAATGGAAACTCTGAGAGCAGCTCCAGTGATGGAGAATCATGCAAGAAGCCAAGGGAAGAGACCATCAAGGCCAAAATTTATAGGTCTTATGTCAGGACCGAACCATCCGATACAACAAGCTTGGTTGTCAAGGATGGATATCAATGGAGAAAATATGGTCAAAAAGTTACAAGGGACAATCCTTGTCCTAGAGCTTACTTCAAGTGCTCGTTTGCTCCAACCTGCCCTGTCAAAAAGAAGGTTCAGAGAAGTGTTGAGGACCAATCAATTCTGGTGGCAACTTATGAAGGTGAACACAACCATCCCAATCCTTCTCAACTTGAAGCATCACCAGGCTCAAACCGCTGCATGACCTTAGGATCAGTCCCCTGCTCAACCTCTCTGGGCACATCCGGACCAACCATCACTCTTGACTTGACAAAATCCAAGTCCAATACTTCGGATACCAAGACCCCGAAACCAAGAACTGAAACACCAGAAGTTCGAAAGCTTTTGGTGGAACAGATGGCGTCTTCCTTGACCAAAGACCCCAACTTCACAAAAGCACTTGCAGCAGCCATTTCAGGAAGATTTCTTCAGCACGATACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0002376 GO:0002474 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006996 GO:0008104 GO:0008150 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019882 GO:0019884 GO:0019886 GO:0022607 GO:0023051 GO:0023056 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031080 GO:0031090 GO:0031410 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031982 GO:0031984 GO:0032006 GO:0032008 GO:0032991 GO:0033036 GO:0034613 GO:0034622 GO:0035459 GO:0035859 GO:0042175 GO:0042802 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048002 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048475 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051668 GO:0061024 GO:0061700 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098827 GO:0098852 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko03013 ko04141 ko04150 map03013 map04141 map04150
Pfam Domains
Protein Families
WD40

Protein Analysis

325

Amino Acids

36.14

Weight (kDa)

8.84

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 172 - 230 1.9e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 614
AccB7I CCANNNNNTGG 1 cut(s) 259
AccIII TCCGGA 1 cut(s) 776
AciI CCGC 1 cut(s) 727
AclWI GGATC 2 cut(s) 48, 751
AcsI RAATTY 1 cut(s) 459
AcuI CTGAAG 1 cut(s) 944
AcyI GRCGYC 1 cut(s) 893
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 2 cut(s) 187, 266
AfiI CCNNNNNNNGG 2 cut(s) 259, 286
AgsI TTSAA 2 cut(s) 586, 707
AjnI CCWGG 2 cut(s) 50, 715
Alw21I GWGCWC 3 cut(s) 135, 294, 593
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 2 cut(s) 48, 751
AlwNI CAGNNNCTG 1 cut(s) 20
Aor13HI TCCGGA 1 cut(s) 776
AoxI GGCC 2 cut(s) 53, 453
ApeKI GCWGC 5 cut(s) 17, 402, 729, 935, 938
ApoI RAATTY 1 cut(s) 459
ArsI GACNNNNNNTTYG 2 cut(s) 797, 829
AspS9I GGNCC 3 cut(s) 480, 643, 779
AsuHPI GGTGA 5 cut(s) 193, 232, 239, 686, 705
AsuII TTCGAA 1 cut(s) 868
AvaII GGWCC 3 cut(s) 480, 643, 779
AxyI CCTNAGG 1 cut(s) 739
BaeGI GKGCMC 1 cut(s) 773
BanII GRGCYC 2 cut(s) 135, 294
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 3 cut(s) 135, 294, 593
BbvI GCAGC 5 cut(s) 4, 414, 716, 947, 950
BccI CCATC 8 cut(s) 217, 407, 455, 496, 512, 693, 794, 883
BciT130I CCWGG 2 cut(s) 52, 717
BciVI GTATCC 1 cut(s) 820
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 1 cut(s) 573
BfuAI ACCTGC 1 cut(s) 614
BfuI GTATCC 1 cut(s) 820
BglI GCCNNNNNGGC 1 cut(s) 61
BisI GCNGC 5 cut(s) 18, 403, 730, 936, 939
BlpI GCTNAGC 1 cut(s) 293
BlsI GCNGC 5 cut(s) 19, 404, 731, 937, 940
Bme1390I CCNGG 2 cut(s) 52, 717
Bme18I GGWCC 3 cut(s) 480, 643, 779
BmgT120I GGNCC 3 cut(s) 480, 643, 779
BmrFI CCNGG 2 cut(s) 52, 717
BmsI GCATC 2 cut(s) 387, 719
BpiI GAAGAC 1 cut(s) 888
BplI GAGNNNNNCTC 2 cut(s) 389, 421
BpmI CTGGAG 1 cut(s) 391
Bpu1102I GCTNAGC 1 cut(s) 293
Bpu14I TTCGAA 1 cut(s) 868
BsaBI GATNNNNATC 1 cut(s) 522
BsaHI GRCGYC 1 cut(s) 893
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 434
BsaWI WCCGGW 1 cut(s) 776
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 286
Bse1I ACTGG 1 cut(s) 408
Bse21I CCTNAGG 1 cut(s) 739
Bse8I GATNNNNATC 1 cut(s) 522
BseAI TCCGGA 1 cut(s) 776
BseBI CCWGG 2 cut(s) 52, 717
BseDI CCNNGG 1 cut(s) 434
BseGI GGATG 5 cut(s) 366, 488, 523, 685, 773
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 2 cut(s) 259, 286
BseMII CTCAG 3 cut(s) 150, 307, 387
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 773
BseXI GCAGC 5 cut(s) 4, 414, 716, 947, 950
BsgI GTGCAG 1 cut(s) 119
BshFI GGCC 2 cut(s) 55, 455
BsiHKAI GWGCWC 3 cut(s) 135, 294, 593
BsiSI CCGG 2 cut(s) 61, 777
BslFI GGGAC 2 cut(s) 572, 734
BslI CCNNNNNNNGG 2 cut(s) 259, 286
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 2 cut(s) 572, 734
BsnI GGCC 2 cut(s) 55, 455
Bso31I GGTCTC 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 4 cut(s) 135, 294, 593, 773
Bsp13I TCCGGA 1 cut(s) 776
Bsp143I GATC 3 cut(s) 40, 84, 743
Bsp1720I GCTNAGC 1 cut(s) 293
BspACI CCGC 1 cut(s) 727
BspANI GGCC 2 cut(s) 55, 455
BspCNI CTCAG 3 cut(s) 149, 306, 388
BspEI TCCGGA 1 cut(s) 776
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 2 cut(s) 48, 751
BspT104I TTCGAA 1 cut(s) 868
BspTNI GGTCTC 1 cut(s) 437
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 434
BssMI GATC 3 cut(s) 40, 84, 743
BssNI GRCGYC 1 cut(s) 893
BssT1I CCWWGG 1 cut(s) 434
Bst2UI CCWGG 2 cut(s) 52, 717
Bst4CI ACNGT 2 cut(s) 220, 356
Bst6I CTCTTC 2 cut(s) 141, 435
BstACI GRCGYC 1 cut(s) 893
BstBI TTCGAA 1 cut(s) 868
BstDEI CTNAG 4 cut(s) 136, 293, 396, 739
BstF5I GGATG 5 cut(s) 366, 488, 523, 685, 773
BstKTI GATC 3 cut(s) 43, 87, 746
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 3 cut(s) 40, 84, 743
BstMWI GCNNNNNNNGC 3 cut(s) 61, 726, 935
BstNI CCWGG 2 cut(s) 52, 717
BstSCI CCNGG 2 cut(s) 50, 715
BstSLI GKGCMC 1 cut(s) 773
BstV1I GCAGC 5 cut(s) 4, 414, 716, 947, 950
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
Bsu36I CCTNAGG 1 cut(s) 739
BsuI GTATCC 1 cut(s) 820
BsuRI GGCC 2 cut(s) 55, 455
BtsCI GGATG 5 cut(s) 366, 488, 523, 685, 773
BtsIMutI CAGTG 2 cut(s) 361, 415
BveI ACCTGC 1 cut(s) 614
CaiI CAGNNNCTG 1 cut(s) 20
Cfr13I GGNCC 3 cut(s) 480, 643, 779
CseI GACGC 1 cut(s) 882
Csp6I GTAC 2 cut(s) 186, 265
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CviAII CATG 3 cut(s) 268, 423, 733
CviQI GTAC 2 cut(s) 186, 265
DdeI CTNAG 4 cut(s) 136, 293, 396, 739
DpnI GATC 3 cut(s) 42, 86, 745
DpnII GATC 3 cut(s) 40, 84, 743
DraIII CACNNNGTG 1 cut(s) 181
Eam1104I CTCTTC 2 cut(s) 141, 435
EarI CTCTTC 2 cut(s) 141, 435
Ecl136II GAGCTC 2 cut(s) 133, 292
Eco130I CCWWGG 1 cut(s) 434
Eco147I AGGCCT 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 135, 294
Eco31I GGTCTC 1 cut(s) 437
Eco32I GATATC 2 cut(s) 303, 524
Eco47I GGWCC 3 cut(s) 480, 643, 779
Eco53kI GAGCTC 2 cut(s) 133, 292
Eco57I CTGAAG 1 cut(s) 944
Eco81I CCTNAGG 1 cut(s) 739
EcoICRI GAGCTC 2 cut(s) 133, 292
EcoRII CCWGG 2 cut(s) 50, 715
EcoRV GATATC 2 cut(s) 303, 524
EcoT14I CCWWGG 1 cut(s) 434
EcoT38I GRGCYC 2 cut(s) 135, 294
ErhI CCWWGG 1 cut(s) 434
FaeI CATG 3 cut(s) 271, 426, 736
FaiI YATR 8 cut(s) 27, 269, 424, 465, 474, 540, 669, 734
FaqI GGGAC 2 cut(s) 572, 734
FatI CATG 3 cut(s) 267, 422, 732
Fnu4HI GCNGC 5 cut(s) 18, 403, 730, 936, 939
FokI GGATG 5 cut(s) 353, 475, 530, 672, 760
FriOI GRGCYC 2 cut(s) 135, 294
Fsp4HI GCNGC 5 cut(s) 18, 403, 730, 936, 939
FspBI CTAG 1 cut(s) 573
GluI GCNGC 5 cut(s) 18, 403, 730, 936, 939
GsuI CTGGAG 1 cut(s) 391
HaeIII GGCC 2 cut(s) 55, 455
HapII CCGG 2 cut(s) 61, 777
HgaI GACGC 1 cut(s) 882
Hin1I GRCGYC 1 cut(s) 893
Hin1II CATG 3 cut(s) 271, 426, 736
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 2 cut(s) 502, 872
HinfI GANTC 2 cut(s) 74, 419
HpaII CCGG 2 cut(s) 61, 777
HphI GGTGA 5 cut(s) 193, 232, 239, 686, 705
Hpy166II GTNNAC 2 cut(s) 216, 677
Hpy188I TCNGA 6 cut(s) 84, 322, 397, 493, 630, 826
Hpy188III TCNNGA 6 cut(s) 38, 88, 478, 777, 794, 948
Hpy8I GTNNAC 2 cut(s) 216, 677
HpyAV CCTTC 5 cut(s) 120, 280, 616, 665, 705
HpyCH4III ACNGT 2 cut(s) 220, 356
HpyCH4V TGCA 5 cut(s) 100, 175, 426, 732, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 726, 935
HpyF3I CTNAG 4 cut(s) 136, 293, 396, 739
Hsp92I GRCGYC 1 cut(s) 893
Hsp92II CATG 3 cut(s) 271, 426, 736
Kpn2I TCCGGA 1 cut(s) 776
Kzo9I GATC 3 cut(s) 40, 84, 743
LmnI GCTCC 5 cut(s) 130, 155, 289, 410, 604
Lsp1109I GCAGC 5 cut(s) 4, 414, 716, 947, 950
LweI GCATC 2 cut(s) 387, 719
MaeI CTAG 1 cut(s) 573
MaeIII GTNAC 2 cut(s) 329, 550
MalI GATC 3 cut(s) 42, 86, 745
MboI GATC 3 cut(s) 40, 84, 743
MboII GAAGA 5 cut(s) 158, 452, 888, 950, 963
MfeI CAATTG 1 cut(s) 254
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 4 cut(s) 135, 294, 593, 773
MluCI AATT 4 cut(s) 170, 254, 459, 651
MmeI TCCRAC 2 cut(s) 240, 626
MnlI CCTC 6 cut(s) 66, 88, 90, 160, 634, 772
MroI TCCGGA 1 cut(s) 776
MseI TTAA 2 cut(s) 45, 372
MspA1I CMGCKG 2 cut(s) 17, 729
MspI CCGG 2 cut(s) 61, 777
MspR9I CCNGG 2 cut(s) 52, 717
MunI CAATTG 1 cut(s) 254
MvaI CCWGG 2 cut(s) 52, 717
MwoI GCNNNNNNNGC 3 cut(s) 61, 726, 935
NdeII GATC 3 cut(s) 40, 84, 743
NlaIII CATG 3 cut(s) 271, 426, 736
NspV TTCGAA 1 cut(s) 868
PceI AGGCCT 1 cut(s) 55
PfeI GAWTC 2 cut(s) 74, 419
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 5 cut(s) 19, 404, 731, 937, 940
Psp124BI GAGCTC 2 cut(s) 135, 294
Psp6I CCWGG 2 cut(s) 50, 715
PspGI CCWGG 2 cut(s) 50, 715
PspPI GGNCC 3 cut(s) 480, 643, 779
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 187, 266
RsaNI GTAC 2 cut(s) 186, 265
SacI GAGCTC 2 cut(s) 135, 294
SaqAI TTAA 2 cut(s) 45, 372
SatI GCNGC 5 cut(s) 18, 403, 730, 936, 939
Sau3AI GATC 3 cut(s) 40, 84, 743
Sau96I GGNCC 3 cut(s) 480, 643, 779
ScrFI CCNGG 2 cut(s) 52, 717
SduI GDGCHC 4 cut(s) 135, 294, 593, 773
SfaNI GCATC 2 cut(s) 387, 719
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 3 cut(s) 480, 643, 779
Sse9I AATT 4 cut(s) 170, 254, 459, 651
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 1 cut(s) 727
SspMI CTAG 1 cut(s) 573
SstI GAGCTC 2 cut(s) 135, 294
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 2 cut(s) 50, 715
StyI CCWWGG 1 cut(s) 434
TaaI ACNGT 2 cut(s) 220, 356
TaqI TCGA 3 cut(s) 69, 117, 868
TaqII GACCGA 1 cut(s) 497
TasI AATT 4 cut(s) 170, 254, 459, 651
TatI WGTACW 1 cut(s) 264
TfiI GAWTC 2 cut(s) 74, 419
Tru1I TTAA 2 cut(s) 45, 372
Tru9I TTAA 2 cut(s) 45, 372
TscAI CASTG 2 cut(s) 361, 415
TseI GCWGC 5 cut(s) 17, 402, 729, 935, 938
TspDTI ATGAA 1 cut(s) 684
TspRI CASTG 2 cut(s) 361, 415
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 3 cut(s) 480, 643, 779
XapI RAATTY 1 cut(s) 459
XspI CTAG 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.