Rh4BG007800

Belongs to the WD repeat SEC13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001675
Physical Location & Seq
Forward (+)
1138213 .. 1138638
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG007800.1

Sequence Viewer

Length: 426 bp
ATGGCTCCTGGTTCTTTAGTTGGAACTGGCATGCTTGAACCTGTTCAGAAGCTGGTCTCTGGTGGCTGCGACAATACAGTGAAGGTGTGGAAATTGTACAATGAGACTTGGAAGATGGATTGCTTCCCTGCTCTTCAAATGCACACTGATTGGGTGAGAGACGTAGCCTGGGCACCCAACTTGGGACTTCCCAAGTCCACCATTGCAAGTTGTTCACAGGACGGAACAGTGGTTATCTGGACTGTGGCCAAAGAAGGCGATCAATGGCAGGGGAAGGTATTGAAGGATTTTAAGACCCCTGTTTGGAGGGTGTCATGGTCACTAACAGGAAACCTACTGGCCGTGGCCGATGGAAATAACCAAGTATCATTGTGGAAAGAGTCTGTTGATGACGAGTGGCAACAAGTGAGCACAGTCGACCCATAG

Protein Analysis

141

Amino Acids

15.65

Weight (kDa)

4.83

Isoelectric Point (pI)

22.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 13 - 86 4.4e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 16 - 126 1.2e-15 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 16 - 127 1.6e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 16 - 126 5.8e-11 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 16 - 128 4.5e-09 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 16 - 128 1e-07 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 16 - 127 6.7e-06 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 17 - 136 1.4e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 44 - 80 3.7e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 417
AcoI YGGCCR 3 cut(s) 246, 339, 345
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 182, 303
AgsI TTSAA 3 cut(s) 38, 137, 283
AjnI CCWGG 2 cut(s) 7, 167
AluBI AGCT 1 cut(s) 52
AluI AGCT 1 cut(s) 52
Alw21I GWGCWC 1 cut(s) 413
Alw26I GTCTC 3 cut(s) 61, 98, 153
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 3 cut(s) 246, 339, 345
ApeKI GCWGC 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 248
BanI GGYRCC 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 413
BbvI GCAGC 1 cut(s) 53
BccI CCATC 2 cut(s) 109, 344
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 2 cut(s) 9, 169
BcoDI GTCTC 3 cut(s) 61, 98, 153
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 9, 169
BmiI GGNNCC 2 cut(s) 6, 174
BmrFI CCNGG 2 cut(s) 9, 169
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 168, 342
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 303
Bse1I ACTGG 2 cut(s) 31, 342
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 2 cut(s) 9, 169
BseDI CCNNGG 2 cut(s) 168, 342
BseLI CCNNNNNNNGG 2 cut(s) 182, 303
BseMI GCAATG 1 cut(s) 201
BseNI ACTGG 2 cut(s) 31, 342
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 1 cut(s) 53
BshFI GGCC 3 cut(s) 248, 341, 347
BshNI GGYRCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 413
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 2 cut(s) 182, 303
BsmAI GTCTC 3 cut(s) 61, 98, 153
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 198
BsnI GGCC 3 cut(s) 248, 341, 347
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 175, 413
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 1 cut(s) 259
BspANI GGCC 3 cut(s) 248, 341, 347
BspLI GGNNCC 2 cut(s) 6, 174
BspQI GCTCTTC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 201
BsrGI TGTACA 1 cut(s) 96
BsrI ACTGG 2 cut(s) 31, 342
BssECI CCNNGG 2 cut(s) 168, 342
BssMI GATC 1 cut(s) 259
Bst2UI CCWGG 2 cut(s) 9, 169
Bst4CI ACNGT 4 cut(s) 79, 229, 244, 415
Bst6I CTCTTC 1 cut(s) 138
BstAUI TGTACA 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 32
BstDSI CCRYGG 1 cut(s) 342
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 3 cut(s) 61, 98, 153
BstMBI GATC 1 cut(s) 259
BstNI CCWGG 2 cut(s) 9, 169
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 7, 167
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 248, 341, 347
BtgI CCRYGG 1 cut(s) 342
BtsIMutI CAGTG 3 cut(s) 84, 144, 234
Cac8I GCNNGC 1 cut(s) 32
CaiI CAGNNNCTG 1 cut(s) 52
Csp6I GTAC 1 cut(s) 97
CviAII CATG 2 cut(s) 31, 315
CviJI RGCY 7 cut(s) 5, 52, 66, 167, 248, 341, 347
CviKI_1 RGCY 7 cut(s) 5, 52, 66, 167, 248, 341, 347
CviQI GTAC 1 cut(s) 97
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
EaeI YGGCCR 3 cut(s) 246, 339, 345
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 61
EcoRII CCWGG 2 cut(s) 7, 167
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 2 cut(s) 34, 318
FaiI YATR 3 cut(s) 32, 316, 424
FaqI GGGAC 1 cut(s) 198
FatI CATG 2 cut(s) 30, 314
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 67
HaeIII GGCC 3 cut(s) 248, 341, 347
Hin1II CATG 2 cut(s) 34, 318
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 1 cut(s) 380
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 3 cut(s) 198, 215, 418
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 1 cut(s) 238
Hpy8I GTNNAC 3 cut(s) 198, 215, 418
HpyAV CCTTC 4 cut(s) 76, 248, 268, 277
HpyCH4III ACNGT 4 cut(s) 79, 229, 244, 415
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 2 cut(s) 142, 206
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 2 cut(s) 34, 318
Kzo9I GATC 1 cut(s) 259
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 1 cut(s) 53
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 318
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 2 cut(s) 124, 125
MhlI GDGCHC 2 cut(s) 175, 413
MlsI TGGCCA 1 cut(s) 248
MluCI AATT 1 cut(s) 92
MluNI TGGCCA 1 cut(s) 248
MlyI GAGTC 1 cut(s) 389
MnlI CCTC 1 cut(s) 300
Mox20I TGGCCA 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 42
MscI TGGCCA 1 cut(s) 248
MseI TTAA 1 cut(s) 291
Msp20I TGGCCA 1 cut(s) 248
MspR9I CCNGG 2 cut(s) 9, 169
MvaI CCWGG 2 cut(s) 9, 169
NdeII GATC 1 cut(s) 259
NlaIII CATG 2 cut(s) 34, 318
NlaIV GGNNCC 2 cut(s) 6, 174
NmuCI GTSAC 1 cut(s) 318
NspI RCATGY 1 cut(s) 34
PaeI GCATGC 1 cut(s) 34
PciSI GCTCTTC 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 42
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 2 cut(s) 7, 167
PspGI CCWGG 2 cut(s) 7, 167
PspN4I GGNNCC 2 cut(s) 6, 174
PstNI CAGNNNCTG 1 cut(s) 52
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SalI GTCGAC 1 cut(s) 416
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 1 cut(s) 259
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 9, 169
SduI GDGCHC 2 cut(s) 175, 413
SetI ASST 6 cut(s) 43, 54, 87, 165, 279, 336
SphI GCATGC 1 cut(s) 34
Sse9I AATT 1 cut(s) 92
StyD4I CCNGG 2 cut(s) 7, 167
TaaI ACNGT 4 cut(s) 79, 229, 244, 415
TaiI ACGT 1 cut(s) 165
TaqI TCGA 1 cut(s) 417
TasI AATT 1 cut(s) 92
TatI WGTACW 1 cut(s) 96
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 3 cut(s) 84, 151, 234
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 1 cut(s) 66
Tsp45I GTSAC 1 cut(s) 318
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 3 cut(s) 84, 151, 234
XceI RCATGY 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.