RLG00000010169

Belongs to the WD repeat SEC13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001675
Physical Location & Seq
Reverse (-)
63813406 .. 63814235
830 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010169

Sequence Viewer

Length: 798 bp
ATGCCGGGGCAGAAGATTGAAACGGGTCACCAGGATGTTGTTCATGATGTTGCCATGGATTACTATGGAAAGCGTCTAGCATCAGCTTCATCTGATATGACCATTAAGATAATTGGTGTCGGCAGCAATGCGGGTTCACAGCACCTTGCTACATTGTCCGGTCACAGAGGTCCTGTATGGCAGGTGGGTTGGGCGCATCCCAAGTTTGGGCCGATCCTTGCTTCGTGCTCGTATGATGGCCAAGTGATAATTTGGAAGGAGGGTAATCCAAATGAGTGGCAACAGGCTCGTGTATTTAATGTGCACAGTTCATCTGTAAATTCCATTTGTTGGGCGCCTCATGAACTTGGCCTCATCTTAGCTTGTGGTTCTTCTGATGGGAACATCTCTGTTTTCACTGCTAGGGCAGATGGTGGTTGGGACACCACTAGGATAGACCAAGAACCTGTTCAGAAGCTGGTCTCTGGTGGCTGCGACAATACAGTGAAGGTGTGGAAATTGTACAATGAGACTTGGAAGATGGATTGCTTCCCTGCTCTTCAAATGCACACTGATTGGGTGAGAGACGTAGCCTGGGCACCCAACTTGGGACTTCCCAAGTCCACCATTGCAAGTTGTTCACAGGACGGAACAGTGGTTATCTGGACTGTGGCCAAAGAAGGCGATCAATGGCAGGGGAAGGTGTCATGGTCACTAACAGGAAACCTACTGGCCGTGGCCGATGGAAATAACGAAGTATCATTGTGGAAAGAGTCTGTTGATGGCGAGTGGCAACAAGTGAGCACAGTCGACCCATAG

Protein Analysis

266

Amino Acids

28.95

Weight (kDa)

5.27

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 6 - 112 2.5e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 8 - 150 7.6e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 86 1.6e-10 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 9 - 135 3.5e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 10 - 130 5.3e-16 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 11 - 132 2.3e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 44 - 132 5.8e-07 Prp19 WD40 domain
WD40 PF00400 47 - 85 3.4e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 70 - 219 3.2e-16 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 70 - 145 1.1e-07 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 95 - 219 2.4e-09 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 144 - 220 2.4e-06 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 146 - 221 4.2e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 151 - 249 2.2e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 151 - 250 3.3e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 2 cut(s) 334, 577
AccB7I CCANNNNNTGG 1 cut(s) 330
AccI GTMKAC 1 cut(s) 789
AciI CCGC 1 cut(s) 131
AclWI GGATC 1 cut(s) 208
AcoI YGGCCR 4 cut(s) 238, 651, 711, 717
AcsI RAATTY 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 335
AfaI GTAC 1 cut(s) 503
AfiI CCNNNNNNNGG 4 cut(s) 206, 207, 330, 587
AgsI TTSAA 2 cut(s) 20, 542
AjnI CCWGG 2 cut(s) 30, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 3 cut(s) 86, 362, 457
AluI AGCT 3 cut(s) 86, 362, 457
Alw21I GWGCWC 3 cut(s) 230, 306, 785
Alw26I GTCTC 3 cut(s) 466, 503, 558
Alw44I GTGCAC 1 cut(s) 302
AlwI GGATC 1 cut(s) 208
AlwNI CAGNNNCTG 1 cut(s) 457
AoxI GGCC 6 cut(s) 209, 238, 349, 651, 711, 717
ApaLI GTGCAC 1 cut(s) 302
ApeKI GCWGC 2 cut(s) 123, 471
ApoI RAATTY 1 cut(s) 319
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 2 cut(s) 196, 337
AspS9I GGNCC 2 cut(s) 170, 209
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 20, 571
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 2 cut(s) 306, 580
BalI TGGCCA 2 cut(s) 240, 653
BanI GGYRCC 2 cut(s) 334, 577
BauI CACGAG 1 cut(s) 288
Bbv12I GWGCWC 3 cut(s) 230, 306, 785
BbvI GCAGC 2 cut(s) 135, 458
BccI CCATC 6 cut(s) 230, 371, 404, 514, 716, 755
BceAI ACGGC 1 cut(s) 698
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 2 cut(s) 32, 574
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 3 cut(s) 466, 503, 558
BfaI CTAG 3 cut(s) 77, 402, 429
BfoI RGCGCY 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 2 cut(s) 124, 472
BlsI GCNGC 2 cut(s) 125, 473
Bme1390I CCNGG 3 cut(s) 6, 32, 574
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 170, 209
BmiI GGNNCC 2 cut(s) 336, 579
BmrFI CCNGG 3 cut(s) 6, 32, 574
BmsI GCATC 2 cut(s) 89, 205
BpuMI CCSGG 1 cut(s) 6
BsaHI GRCGYC 1 cut(s) 335
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 4 cut(s) 5, 54, 573, 714
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 4 cut(s) 206, 207, 330, 587
Bse1I ACTGG 1 cut(s) 714
Bse3DI GCAATG 2 cut(s) 133, 606
BseBI CCWGG 2 cut(s) 32, 574
BseDI CCNNGG 4 cut(s) 5, 54, 573, 714
BseGI GGATG 2 cut(s) 40, 196
BseLI CCNNNNNNNGG 4 cut(s) 206, 207, 330, 587
BseMI GCAATG 2 cut(s) 133, 606
BseNI ACTGG 1 cut(s) 714
BseSI GKGCMC 2 cut(s) 306, 580
BseXI GCAGC 2 cut(s) 135, 458
BshFI GGCC 6 cut(s) 211, 240, 351, 653, 713, 719
BshNI GGYRCC 2 cut(s) 334, 577
BsiHKAI GWGCWC 3 cut(s) 230, 306, 785
BsiSI CCGG 2 cut(s) 5, 159
BslFI GGGAC 2 cut(s) 434, 603
BslI CCNNNNNNNGG 4 cut(s) 206, 207, 330, 587
BsmAI GTCTC 3 cut(s) 466, 503, 558
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 2 cut(s) 434, 603
BsnI GGCC 6 cut(s) 211, 240, 351, 653, 713, 719
Bso31I GGTCTC 1 cut(s) 466
Bsp1286I GDGCHC 4 cut(s) 230, 306, 580, 785
Bsp1407I TGTACA 1 cut(s) 501
Bsp143I GATC 2 cut(s) 213, 664
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 1 cut(s) 131
BspANI GGCC 6 cut(s) 211, 240, 351, 653, 713, 719
BspHI TCATGA 2 cut(s) 43, 340
BspLI GGNNCC 2 cut(s) 336, 579
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 1 cut(s) 208
BspQI GCTCTTC 1 cut(s) 543
BspT107I GGYRCC 2 cut(s) 334, 577
BspTNI GGTCTC 1 cut(s) 466
BsrDI GCAATG 2 cut(s) 133, 606
BsrGI TGTACA 1 cut(s) 501
BsrI ACTGG 1 cut(s) 714
BssECI CCNNGG 4 cut(s) 5, 54, 573, 714
BssMI GATC 2 cut(s) 213, 664
BssNI GRCGYC 1 cut(s) 335
BssSI CACGAG 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 54
Bst2BI CACGAG 1 cut(s) 288
Bst2UI CCWGG 2 cut(s) 32, 574
Bst4CI ACNGT 5 cut(s) 308, 484, 634, 649, 787
Bst6I CTCTTC 1 cut(s) 543
BstACI GRCGYC 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 501
BstDEI CTNAG 1 cut(s) 358
BstDSI CCRYGG 2 cut(s) 54, 714
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 2 cut(s) 40, 196
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 2 cut(s) 196, 337
BstKTI GATC 2 cut(s) 216, 667
BstMAI GTCTC 3 cut(s) 466, 503, 558
BstMBI GATC 2 cut(s) 213, 664
BstNI CCWGG 2 cut(s) 32, 574
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 3 cut(s) 4, 30, 572
BstSLI GKGCMC 2 cut(s) 306, 580
BstV1I GCAGC 2 cut(s) 135, 458
BstXI CCANNNNNNTGG 1 cut(s) 276
BsuRI GGCC 6 cut(s) 211, 240, 351, 653, 713, 719
BtgI CCRYGG 2 cut(s) 54, 714
BtsCI GGATG 2 cut(s) 40, 196
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 4 cut(s) 396, 489, 549, 639
BveI ACCTGC 1 cut(s) 172
CaiI CAGNNNCTG 1 cut(s) 457
CciI TCATGA 2 cut(s) 43, 340
CfoI GCGC 2 cut(s) 196, 337
Cfr13I GGNCC 2 cut(s) 170, 209
CseI GACGC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 502
CviAII CATG 4 cut(s) 44, 55, 341, 687
CviQI GTAC 1 cut(s) 502
DdeI CTNAG 1 cut(s) 358
DinI GGCGCC 1 cut(s) 336
DpnI GATC 2 cut(s) 215, 666
DpnII GATC 2 cut(s) 213, 664
EaeI YGGCCR 4 cut(s) 238, 651, 711, 717
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 170
EcoO65I GGTNACC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 30, 572
EcoT14I CCWWGG 1 cut(s) 54
EgeI GGCGCC 1 cut(s) 336
EheI GGCGCC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 4 cut(s) 47, 58, 344, 690
FaiI YATR 9 cut(s) 45, 56, 66, 98, 178, 234, 342, 688, 796
FaqI GGGAC 2 cut(s) 434, 603
FatI CATG 4 cut(s) 43, 54, 340, 686
FauI CCCGC 1 cut(s) 124
FblI GTMKAC 1 cut(s) 789
Fnu4HI GCNGC 2 cut(s) 124, 472
FokI GGATG 2 cut(s) 47, 183
Fsp4HI GCNGC 2 cut(s) 124, 472
FspBI CTAG 3 cut(s) 77, 402, 429
GlaI GCGC 2 cut(s) 195, 336
GluI GCNGC 2 cut(s) 124, 472
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 6 cut(s) 211, 240, 351, 653, 713, 719
HapII CCGG 2 cut(s) 5, 159
HgaI GACGC 1 cut(s) 62
HhaI GCGC 2 cut(s) 196, 337
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 4 cut(s) 47, 58, 344, 690
Hin6I GCGC 2 cut(s) 194, 335
HinP1I GCGC 2 cut(s) 194, 335
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 1 cut(s) 752
HpaII CCGG 2 cut(s) 5, 159
HphI GGTGA 2 cut(s) 20, 571
Hpy166II GTNNAC 5 cut(s) 137, 304, 603, 620, 790
Hpy188I TCNGA 3 cut(s) 94, 376, 453
Hpy188III TCNNGA 3 cut(s) 44, 341, 643
Hpy8I GTNNAC 5 cut(s) 137, 304, 603, 620, 790
HpyAV CCTTC 4 cut(s) 250, 481, 653, 673
HpyCH4III ACNGT 5 cut(s) 308, 484, 634, 649, 787
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 3 cut(s) 304, 547, 611
HpyF3I CTNAG 1 cut(s) 358
HpySE526I ACGT 1 cut(s) 567
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 4 cut(s) 47, 58, 344, 690
HspAI GCGC 2 cut(s) 194, 335
KasI GGCGCC 1 cut(s) 334
Kzo9I GATC 2 cut(s) 213, 664
LguI GCTCTTC 1 cut(s) 543
Lsp1109I GCAGC 2 cut(s) 135, 458
LweI GCATC 2 cut(s) 89, 205
MaeI CTAG 3 cut(s) 77, 402, 429
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 3 cut(s) 26, 161, 690
MalI GATC 2 cut(s) 215, 666
MboI GATC 2 cut(s) 213, 664
MboII GAAGA 4 cut(s) 25, 363, 529, 530
MhlI GDGCHC 4 cut(s) 230, 306, 580, 785
MlsI TGGCCA 2 cut(s) 240, 653
MluCI AATT 4 cut(s) 111, 249, 319, 497
MluNI TGGCCA 2 cut(s) 240, 653
Mly113I GGCGCC 1 cut(s) 335
MlyI GAGTC 1 cut(s) 761
MnlI CCTC 4 cut(s) 161, 253, 348, 362
Mox20I TGGCCA 2 cut(s) 240, 653
MroXI GAANNNNTTC 1 cut(s) 447
MscI TGGCCA 2 cut(s) 240, 653
MseI TTAA 2 cut(s) 105, 297
MslI CAYNNNNRTG 1 cut(s) 33
Msp20I TGGCCA 2 cut(s) 240, 653
MspI CCGG 2 cut(s) 5, 159
MspR9I CCNGG 3 cut(s) 6, 32, 574
MvaI CCWGG 2 cut(s) 32, 574
NarI GGCGCC 1 cut(s) 335
NciI CCSGG 1 cut(s) 6
NcoI CCATGG 1 cut(s) 54
NdeII GATC 2 cut(s) 213, 664
NlaIII CATG 4 cut(s) 47, 58, 344, 690
NlaIV GGNNCC 2 cut(s) 336, 579
NmuCI GTSAC 3 cut(s) 26, 161, 690
PagI TCATGA 2 cut(s) 43, 340
PaqCI CACCTGC 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 447
PflMI CCANNNNNTGG 1 cut(s) 330
PkrI GCNGC 2 cut(s) 125, 473
PleI GAGTC 1 cut(s) 760
PluTI GGCGCC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 760
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 30, 572
PspEI GGTNACC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 30, 572
PspN4I GGNNCC 2 cut(s) 336, 579
PspPI GGNCC 2 cut(s) 170, 209
PspPPI RGGWCCY 1 cut(s) 170
PstNI CAGNNNCTG 1 cut(s) 457
RsaI GTAC 1 cut(s) 503
RsaNI GTAC 1 cut(s) 502
RseI CAYNNNNRTG 1 cut(s) 33
SalI GTCGAC 1 cut(s) 788
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 2 cut(s) 105, 297
SatI GCNGC 2 cut(s) 124, 472
Sau3AI GATC 2 cut(s) 213, 664
Sau96I GGNCC 2 cut(s) 170, 209
SchI GAGTC 1 cut(s) 761
ScrFI CCNGG 3 cut(s) 6, 32, 574
SduI GDGCHC 4 cut(s) 230, 306, 580, 785
SfaNI GCATC 2 cut(s) 89, 205
SfoI GGCGCC 1 cut(s) 336
SinI GGWCC 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 4 cut(s) 111, 249, 319, 497
SsiI CCGC 1 cut(s) 131
SspDI GGCGCC 1 cut(s) 334
SspMI CTAG 3 cut(s) 77, 402, 429
StyD4I CCNGG 3 cut(s) 4, 30, 572
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 5 cut(s) 308, 484, 634, 649, 787
TaiI ACGT 1 cut(s) 570
TaqI TCGA 1 cut(s) 789
TasI AATT 4 cut(s) 111, 249, 319, 497
TatI WGTACW 1 cut(s) 501
Tru1I TTAA 2 cut(s) 105, 297
Tru9I TTAA 2 cut(s) 105, 297
TscAI CASTG 4 cut(s) 403, 489, 556, 639
TseFI GTSAC 3 cut(s) 26, 161, 690
TseI GCWGC 2 cut(s) 123, 471
Tsp45I GTSAC 3 cut(s) 26, 161, 690
TspDTI ATGAA 4 cut(s) 32, 78, 300, 357
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 4 cut(s) 403, 489, 556, 639
Van91I CCANNNNNTGG 1 cut(s) 330
VneI GTGCAC 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 789
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 3 cut(s) 77, 402, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.