Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001675

Physical Location & Seq

Genomic Coordinates Forward (+)

3101074 .. 3103384

Gene Structure 2311 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1042300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGCCTGCCCAGAAGATCGAAACGGGTCACCAGGACACTGTCCACGATGTAGCCATGGATTACTATGGAAAGCGTCTAGCAACAGCTTCGTCTGATGCCACCATTAAGATAATTGGTGTCGGAAACGCTTCACAGCACCTTGCTACATTGTCAGCTCATAGAGGCCCTGTCTGGGAGGTAGCTTGGGCGCATCCCAAGTTTGGGTCGATTCTTGCTTCTTGTTCATATGATGGGCAGGTGATCATTTGGAAGGAGGGAAATCCCAATGAGTGGCAACAGGCTCATGTTTTTAACGACCACAAGTCATCGGTAAATTCCATTGCTTGGGCACCTCATGAACTTGGTCTGAGCTTAGCTTGCGGTTCTTCTGATGGGAATATTTCGGTTTTCACTGCCAGGGCCGATGGTGGTTGGGACACCACTAAGATAGATCAAGCCCACCCTGTTGGAGTAACCTCAGTTTCATGGGCTCCATCGATGGCTCCTGGTGCTCTAGTTGGATCTGGTCTGCTTGATCCAGTTCATAAGCTGGCATCCGGTGGGTGTGATAATACTGTGAAGGTGTGGAAGCTGTACAATGGGATCTGGAAGATGGACTGCTTCCCTGCCCTTCAAATGCACAGCGATTGGGTGAGAGATGTAGCATGGGCACCCAACTTGGGACTTCCAAAGTCCACAATCGCAAGTGCTTCACAGGACGGAACAGTGGTGATTTGGACTGTGGCCAAGGAAGGTGAGCAATGGGAGGGGAAGGTTTTGAAGGATTTTAAGACCCCGGTCTGGAGGGTGTCGTGGTCTCTAACCGGAAACTTGCTGGCTGTGGCTGGTGGAAATAACAATGTAACATTGTGGAAAGAATCTGTCGACGGTGAGTGGCAACAAGTAAGCACAGTTGAGCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005829 GO:0006810 GO:0006888 GO:0008150 GO:0012505 GO:0016020 GO:0016192 GO:0031080 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0098588 GO:0098805 GO:0098852

KEGG Pathways

Metabolic & Signaling

ko03013 ko04141 ko04150 map03013 map04141 map04150

Pfam Domains

Protein Families

WD40

Protein Analysis

301

Amino Acids

32.39

Weight (kDa)

5.84

Isoelectric Point (pI)

27.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_TEP1_2nd	PF25047	4 - 189	8.6e-08	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	5 - 112	1.3e-11	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	8 - 157	1.1e-16	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	8 - 84	4.6e-10	WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	9 - 134	3.9e-06	MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz	PF24807	9 - 157	3.3e-13	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	10 - 87	7e-12	THOC3 beta-propeller domain
WD40_Prp19	PF24814	14 - 169	9.7e-12	Prp19 WD40 domain
WD40	PF00400	45 - 83	3.5e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	48 - 133	6.5e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	92 - 285	1.5e-13	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	118 - 243	1.5e-07	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	173 - 246	5.3e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	175 - 285	7e-15	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	176 - 287	5.1e-08	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	176 - 285	3.8e-14	WDR5 beta-propeller domain
WD40_Prp19	PF24814	176 - 284	2e-07	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001675)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30050 AT3G01340 AT3G01340
fragaria_vesca	FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g49840 FvH4_6g49840 FvH4_6g49840
malus_domestica	MD09G1041000.v1.1 MD09G1205500.v1.1 MD17G1042300.v1.1 MD17G1185700.v1.1
prunus_persica	Prupe.3G060800_v2.0.a1 Prupe.3G276700_v2.0.a1
pyrus_communis	pycom09g12210 pycom111g03200 pycom17g03790 pycom17g19510
rosa_chinensis	RchiOBHm_Chr2g0140101 RchiOBHm_Chr2g0170031
rosa_laevigata	RLG00000010169 RLG00000019849 RLG00000021917
rosa_multiflora	Rmu_co8506925.1_g000001 Rmu_sc0003244.1_g000028 Rmu_sc0013078.1_g000015 Rmu_ssc0000389.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081680 Rroxscaffold_2G00105060
rosa_rugosa	Rorug02G0361100 Rorug02G0361200 Rorug02G0545000 Rorug02G0545100
rosa_samantha	Rh2AG411800 Rh2AG617500 Rh2BG422100 Rh2BG628800 Rh2CG397900 Rh2CG598300 Rh2DG431500 Rh2DG640000 Rh2DG640600 Rh4BG007800
rosa_wichuraiana	Rw2G033740 Rw2G051120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	226
Acc36I	ACCTGC	1 cut(s)	226
AccB1I	GGYRCC	2 cut(s)	328, 649
AccB7I	CCANNNNNTGG	2 cut(s)	270, 324
AccI	GTMKAC	1 cut(s)	864
AciI	CCGC	1 cut(s)	360
AclWI	GGATC	3 cut(s)	508, 509, 590
AcoI	YGGCCR	1 cut(s)	723
AcsI	RAATTY	1 cut(s)	313
AfaI	GTAC	1 cut(s)	575
AfiI	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 270, 324, 659, 780
AgsI	TTSAA	2 cut(s)	614, 760
AhdI	GACNNNNNGTC	1 cut(s)	301
AjnI	CCWGG	3 cut(s)	30, 395, 484
AluBI	AGCT	7 cut(s)	86, 155, 182, 351, 356, 529, 571
AluI	AGCT	7 cut(s)	86, 155, 182, 351, 356, 529, 571
Alw21I	GWGCWC	1 cut(s)	493
Alw26I	GTCTC	1 cut(s)	801
AlwI	GGATC	3 cut(s)	508, 509, 590
AoxI	GGCC	3 cut(s)	163, 399, 723
ApoI	RAATTY	1 cut(s)	313
Asp700I	GAANNNNTTC	1 cut(s)	127
AspLEI	GCGC	1 cut(s)	190
AspS9I	GGNCC	2 cut(s)	164, 399
AsuC2I	CCSGG	1 cut(s)	776
AsuHPI	GGTGA	6 cut(s)	20, 250, 643, 721, 746, 881
BaeGI	GKGCMC	2 cut(s)	331, 652
BalI	TGGCCA	1 cut(s)	725
BanI	GGYRCC	2 cut(s)	328, 649
BanII	GRGCYC	1 cut(s)	472
Bbv12I	GWGCWC	1 cut(s)	493
BccI	CCATC	6 cut(s)	224, 365, 398, 472, 481, 586
BcgI	CGANNNNNNTGC	2 cut(s)	69, 103
BciT130I	CCWGG	3 cut(s)	32, 397, 486
BclI	TGATCA	1 cut(s)	240
BcnI	CCSGG	1 cut(s)	776
BcoDI	GTCTC	1 cut(s)	801
BfaI	CTAG	2 cut(s)	77, 494
BfuAI	ACCTGC	1 cut(s)	226
BlpI	GCTNAGC	1 cut(s)	352
Bme1390I	CCNGG	4 cut(s)	32, 397, 486, 776
BmeRI	GACNNNNNGTC	1 cut(s)	301
BmgT120I	GGNCC	2 cut(s)	164, 399
BmiI	GGNNCC	4 cut(s)	330, 471, 483, 651
BmrFI	CCNGG	4 cut(s)	32, 397, 486, 776
BmsI	GCATC	3 cut(s)	85, 199, 542
BoxI	GACNNNNGTC	1 cut(s)	776
BpmI	CTGGAG	1 cut(s)	802
Bpu1102I	GCTNAGC	1 cut(s)	352
BpuMI	CCSGG	1 cut(s)	776
Bsa29I	ATCGAT	1 cut(s)	476
BsaI	GGTCTC	1 cut(s)	801
BsaJI	CCNNGG	4 cut(s)	54, 396, 726, 774
BsaWI	WCCGGW	2 cut(s)	536, 803
Bsc4I	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 270, 324, 659, 780
Bse1I	ACTGG	1 cut(s)	518
Bse3DI	GCAATG	2 cut(s)	318, 746
BseBI	CCWGG	3 cut(s)	32, 397, 486
BseCI	ATCGAT	1 cut(s)	476
BseDI	CCNNGG	4 cut(s)	54, 396, 726, 774
BseGI	GGATG	2 cut(s)	190, 533
BseLI	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 270, 324, 659, 780
BseMI	GCAATG	2 cut(s)	318, 746
BseMII	CTCAG	2 cut(s)	338, 471
BseNI	ACTGG	1 cut(s)	518
BseSI	GKGCMC	2 cut(s)	331, 652
BshFI	GGCC	3 cut(s)	165, 401, 725
BshNI	GGYRCC	2 cut(s)	328, 649
BshVI	ATCGAT	1 cut(s)	476
BsiHKAI	GWGCWC	1 cut(s)	493
BsiSI	CCGG	3 cut(s)	537, 776, 804
BslFI	GGGAC	2 cut(s)	428, 675
BslI	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 270, 324, 659, 780
BsmAI	GTCTC	1 cut(s)	801
BsmFI	GGGAC	2 cut(s)	428, 675
BsnI	GGCC	3 cut(s)	165, 401, 725
Bso31I	GGTCTC	1 cut(s)	801
Bsp1286I	GDGCHC	4 cut(s)	331, 472, 493, 652
Bsp1407I	TGTACA	1 cut(s)	573
Bsp143I	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
Bsp1720I	GCTNAGC	1 cut(s)	352
Bsp19I	CCATGG	1 cut(s)	54
BspACI	CCGC	1 cut(s)	360
BspANI	GGCC	3 cut(s)	165, 401, 725
BspCNI	CTCAG	2 cut(s)	339, 470
BspDI	ATCGAT	1 cut(s)	476
BspHI	TCATGA	1 cut(s)	334
BspLI	GGNNCC	4 cut(s)	330, 471, 483, 651
BspMI	ACCTGC	1 cut(s)	226
BspPI	GGATC	3 cut(s)	508, 509, 590
BspT107I	GGYRCC	2 cut(s)	328, 649
BspTNI	GGTCTC	1 cut(s)	801
BsrDI	GCAATG	2 cut(s)	318, 746
BsrGI	TGTACA	1 cut(s)	573
BsrI	ACTGG	1 cut(s)	518
BssECI	CCNNGG	4 cut(s)	54, 396, 726, 774
BssMI	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
BssT1I	CCWWGG	2 cut(s)	54, 726
Bst2UI	CCWGG	3 cut(s)	32, 397, 486
Bst4CI	ACNGT	6 cut(s)	40, 556, 706, 721, 869, 892
BstAUI	TGTACA	1 cut(s)	573
BstC8I	GCNNGC	4 cut(s)	6, 358, 531, 816
BstDEI	CTNAG	4 cut(s)	347, 352, 423, 457
BstDSI	CCRYGG	1 cut(s)	54
BstEII	GGTNACC	1 cut(s)	26
BstF5I	GGATG	2 cut(s)	190, 533
BstHHI	GCGC	1 cut(s)	190
BstKTI	GATC	6 cut(s)	18, 243, 433, 503, 517, 585
BstMAI	GTCTC	1 cut(s)	801
BstMBI	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
BstMWI	GCNNNNNNNGC	2 cut(s)	357, 488
BstNI	CCWGG	3 cut(s)	32, 397, 486
BstPAI	GACNNNNGTC	1 cut(s)	776
BstPI	GGTNACC	1 cut(s)	26
BstSCI	CCNGG	4 cut(s)	30, 395, 484, 774
BstSLI	GKGCMC	2 cut(s)	331, 652
BstX2I	RGATCY	2 cut(s)	500, 582
BstXI	CCANNNNNNTGG	1 cut(s)	446
BstYI	RGATCY	2 cut(s)	500, 582
Bsu15I	ATCGAT	1 cut(s)	476
BsuRI	GGCC	3 cut(s)	165, 401, 725
BsuTUI	ATCGAT	1 cut(s)	476
BtgI	CCRYGG	1 cut(s)	54
BtsCI	GGATG	2 cut(s)	190, 533
BtsI	GCAGTG	1 cut(s)	390
BtsIMutI	CAGTG	3 cut(s)	36, 390, 711
BveI	ACCTGC	1 cut(s)	226
Cac8I	GCNNGC	4 cut(s)	6, 358, 531, 816
CciI	TCATGA	1 cut(s)	334
CfoI	GCGC	1 cut(s)	190
Cfr13I	GGNCC	2 cut(s)	164, 399
ClaI	ATCGAT	1 cut(s)	476
CseI	GACGC	1 cut(s)	62
Csp6I	GTAC	1 cut(s)	574
CviAII	CATG	5 cut(s)	55, 284, 335, 465, 645
CviQI	GTAC	1 cut(s)	574
DdeI	CTNAG	4 cut(s)	347, 352, 423, 457
DpnI	GATC	6 cut(s)	17, 242, 432, 502, 516, 584
DpnII	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
DriI	GACNNNNNGTC	1 cut(s)	301
EaeI	YGGCCR	1 cut(s)	723
Eam1105I	GACNNNNNGTC	1 cut(s)	301
Eco130I	CCWWGG	2 cut(s)	54, 726
Eco24I	GRGCYC	1 cut(s)	472
Eco31I	GGTCTC	1 cut(s)	801
Eco91I	GGTNACC	1 cut(s)	26
EcoO109I	RGGNCCY	1 cut(s)	164
EcoO65I	GGTNACC	1 cut(s)	26
EcoRII	CCWGG	3 cut(s)	30, 395, 484
EcoT14I	CCWWGG	2 cut(s)	54, 726
EcoT38I	GRGCYC	1 cut(s)	472
ErhI	CCWWGG	2 cut(s)	54, 726
FaeI	CATG	5 cut(s)	58, 287, 338, 468, 648
FaqI	GGGAC	2 cut(s)	428, 675
FatI	CATG	5 cut(s)	54, 283, 334, 464, 644
FauNDI	CATATG	1 cut(s)	226
FbaI	TGATCA	1 cut(s)	240
FblI	GTMKAC	1 cut(s)	864
FokI	GGATG	2 cut(s)	177, 520
FriOI	GRGCYC	1 cut(s)	472
FspBI	CTAG	2 cut(s)	77, 494
GlaI	GCGC	1 cut(s)	189
GsuI	CTGGAG	1 cut(s)	802
HaeIII	GGCC	3 cut(s)	165, 401, 725
HapII	CCGG	3 cut(s)	537, 776, 804
HgaI	GACGC	1 cut(s)	62
HhaI	GCGC	1 cut(s)	190
Hin1II	CATG	5 cut(s)	58, 287, 338, 468, 648
Hin6I	GCGC	1 cut(s)	188
HinP1I	GCGC	1 cut(s)	188
HincII	GTYRAC	1 cut(s)	865
HindII	GTYRAC	1 cut(s)	865
HinfI	GANTC	2 cut(s)	208, 857
HpaII	CCGG	3 cut(s)	537, 776, 804
HphI	GGTGA	6 cut(s)	20, 250, 643, 721, 746, 881
Hpy166II	GTNNAC	3 cut(s)	43, 675, 865
Hpy188I	TCNGA	4 cut(s)	94, 122, 348, 370
Hpy188III	TCNNGA	3 cut(s)	335, 586, 781
Hpy8I	GTNNAC	3 cut(s)	43, 675, 865
Hpy99I	CGWCG	1 cut(s)	869
HpyAV	CCTTC	6 cut(s)	244, 553, 620, 725, 745, 754
HpyCH4III	ACNGT	6 cut(s)	40, 556, 706, 721, 869, 892
HpyCH4V	TGCA	1 cut(s)	619
HpyF10VI	GCNNNNNNNGC	2 cut(s)	357, 488
HpyF3I	CTNAG	4 cut(s)	347, 352, 423, 457
Hsp92II	CATG	5 cut(s)	58, 287, 338, 468, 648
HspAI	GCGC	1 cut(s)	188
Ksp22I	TGATCA	1 cut(s)	240
Kzo9I	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
LmnI	GCTCC	2 cut(s)	475, 487
LweI	GCATC	3 cut(s)	85, 199, 542
MaeI	CTAG	2 cut(s)	77, 494
MaeIII	GTNAC	3 cut(s)	26, 451, 841
MalI	GATC	6 cut(s)	17, 242, 432, 502, 516, 584
MboI	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
MboII	GAAGA	3 cut(s)	25, 357, 601
MflI	RGATCY	2 cut(s)	500, 582
MhlI	GDGCHC	4 cut(s)	331, 472, 493, 652
MlsI	TGGCCA	1 cut(s)	725
MluCI	AATT	2 cut(s)	111, 313
MluNI	TGGCCA	1 cut(s)	725
MmeI	TCCRAC	3 cut(s)	100, 427, 478
MnlI	CCTC	7 cut(s)	155, 169, 247, 342, 466, 739, 777
Mox20I	TGGCCA	1 cut(s)	725
MroXI	GAANNNNTTC	1 cut(s)	127
MscI	TGGCCA	1 cut(s)	725
MseI	TTAA	3 cut(s)	105, 291, 768
Msp20I	TGGCCA	1 cut(s)	725
MspI	CCGG	3 cut(s)	537, 776, 804
MspR9I	CCNGG	4 cut(s)	32, 397, 486, 776
MvaI	CCWGG	3 cut(s)	32, 397, 486
MwoI	GCNNNNNNNGC	2 cut(s)	357, 488
NciI	CCSGG	1 cut(s)	776
NcoI	CCATGG	1 cut(s)	54
NdeI	CATATG	1 cut(s)	226
NdeII	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
NlaIII	CATG	5 cut(s)	58, 287, 338, 468, 648
NlaIV	GGNNCC	4 cut(s)	330, 471, 483, 651
NmuCI	GTSAC	1 cut(s)	26
PagI	TCATGA	1 cut(s)	334
PaqCI	CACCTGC	1 cut(s)	226
PdmI	GAANNNNTTC	1 cut(s)	127
PfeI	GAWTC	2 cut(s)	208, 857
PflFI	GACNNNGTC	1 cut(s)	38
PflMI	CCANNNNNTGG	2 cut(s)	270, 324
PshAI	GACNNNNGTC	1 cut(s)	776
Psp6I	CCWGG	3 cut(s)	30, 395, 484
PspEI	GGTNACC	1 cut(s)	26
PspGI	CCWGG	3 cut(s)	30, 395, 484
PspN4I	GGNNCC	4 cut(s)	330, 471, 483, 651
PspPI	GGNCC	2 cut(s)	164, 399
PsuI	RGATCY	2 cut(s)	500, 582
PsyI	GACNNNGTC	1 cut(s)	38
RsaI	GTAC	1 cut(s)	575
RsaNI	GTAC	1 cut(s)	574
SalI	GTCGAC	1 cut(s)	863
SaqAI	TTAA	3 cut(s)	105, 291, 768
Sau3AI	GATC	6 cut(s)	15, 240, 430, 500, 514, 582
Sau96I	GGNCC	2 cut(s)	164, 399
ScrFI	CCNGG	4 cut(s)	32, 397, 486, 776
SduI	GDGCHC	4 cut(s)	331, 472, 493, 652
SfaNI	GCATC	3 cut(s)	85, 199, 542
Sse9I	AATT	2 cut(s)	111, 313
SsiI	CCGC	1 cut(s)	360
SspI	AATATT	1 cut(s)	379
SspMI	CTAG	2 cut(s)	77, 494
StyD4I	CCNGG	4 cut(s)	30, 395, 484, 774
StyI	CCWWGG	2 cut(s)	54, 726
TaaI	ACNGT	6 cut(s)	40, 556, 706, 721, 869, 892
TaqI	TCGA	4 cut(s)	18, 206, 476, 864
TasI	AATT	2 cut(s)	111, 313
TatI	WGTACW	1 cut(s)	573
TfiI	GAWTC	2 cut(s)	208, 857
Tru1I	TTAA	3 cut(s)	105, 291, 768
Tru9I	TTAA	3 cut(s)	105, 291, 768
TscAI	CASTG	3 cut(s)	43, 397, 711
TseFI	GTSAC	1 cut(s)	26
Tsp45I	GTSAC	1 cut(s)	26
TspDTI	ATGAA	4 cut(s)	213, 351, 453, 512
TspGWI	ACGGA	1 cut(s)	714
TspRI	CASTG	3 cut(s)	43, 397, 711
Tth111I	GACNNNGTC	1 cut(s)	38
Van91I	CCANNNNNTGG	2 cut(s)	270, 324
XapI	RAATTY	1 cut(s)	313
XmiI	GTMKAC	1 cut(s)	864
XmnI	GAANNNNTTC	1 cut(s)	127
XspI	CTAG	2 cut(s)	77, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.