MD09G1205500.v1.1

Belongs to the WD repeat SEC13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001675
Physical Location & Seq
Forward (+)
19590444 .. 19592039
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1205500.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGCGAGTTTCTTGGTTTCACAGCAGCAGAGCAATTCGCTATCCACTTCGTCCTGAAACTACGTCCTTTCGATCCCAATCTTTGCTTCCCTGGAGTAGAGAAATGCCTGCACAAAAGGTTGAGACTGGCCACCAGGACACAGTCCATGATGTGGTCATGGATTACTATGGTAAGCGCCTCGCCACAGCTTCATCCGACAACACAATTAAGATAATTGGGGTGAGCAATTCATCCTCTCAGCATCTAGCAACCCTGACTGGTCACCAGGGACCAGTTTGGCAGGTTGCTTGGGCTCATCCAAAATTCGGGTCTTTACTTGCTTCCTGTTCTTATGATGGGCGTGTCATACTGTGGAAGGAAGGTAATCAGAATGAGTGGACCCAAGCTCATGTTTTTGATGATCACAAATCCTCTGTGAACTCAATTGCTTGGGCTCCTCATGAACTCGGTCTTTGTTTGGCATGTGGTTCTTCTGATGGGAACATCTCAGTTTTCACTGCAAGGTCTGATGGTGGCTGGGATACCTCGAGGATCGATCAAGCTCACCCAGTTGGTGTCACCTCTGTTTCTTGGGCTCCCTCAACAGCCCCCGGTGCTCTTGTTGGTTCTGGTCTGCTTGACCCTGTCCAGAAGCTGTGTTCTGGTGGTTGTGATAACACTGTAAAAGTGTGGAAGCTTGGTAATGGCATTTGGAAGCTGGACTGCTTTCCGGCTCTTCATATGCATGTTGATTGGGTCAGGGATGTTGCTTGGGCACCCAACTTGGGACTACCAAAATCAACCATTGCCAGTGCCTCACAGGATGGTAAAGTGATTATATGGACCGTGGGCAAGGATGGGGATCAATGGGAAGGTAAGGTGCTGCATGATTTCAATGCACCTGTTTGGAGGGTCTCATGGTCGCTGACTGGAAACATATTGGCTGTGGCTGATGGGAACAACAGCGTGACATTATGGAAGGAAGCAGTAGATGGGGAATGGCAACAGGTGACAACAGTTGACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0002376 GO:0002474 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006996 GO:0008104 GO:0008150 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019882 GO:0019884 GO:0019886 GO:0022607 GO:0023051 GO:0023056 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031080 GO:0031090 GO:0031410 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031982 GO:0031984 GO:0032006 GO:0032008 GO:0032991 GO:0033036 GO:0034613 GO:0034622 GO:0035459 GO:0035859 GO:0042175 GO:0042802 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048002 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048475 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051668 GO:0061024 GO:0061700 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098827 GO:0098852 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko03013 ko04141 ko04150 map03013 map04141 map04150
Pfam Domains
Protein Families
WD40

Protein Analysis

336

Amino Acids

36.65

Weight (kDa)

6.21

Isoelectric Point (pI)

31.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 33 - 224 2.1e-07 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 38 - 143 4.4e-12 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 40 - 168 4.3e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR5 PF25175 42 - 192 8.1e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 42 - 124 1.1e-10 WDR3 first beta-propeller domain
WDR55 PF24796 42 - 319 9e-07 WDR55
WD40_CDC20-Fz PF24807 43 - 193 5e-14 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 44 - 194 8.8e-19 THOC3 beta-propeller domain
WD40_Prp19 PF24814 57 - 204 1.1e-12 Prp19 WD40 domain
WD40_Gbeta PF25391 59 - 194 7.4e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR35_TULP_N PF24797 77 - 193 1.8e-07 WDR35/TULP4 N-terminal
WD40 PF00400 80 - 118 1.7e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 82 - 169 8.4e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 127 - 320 3.1e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 210 - 320 1.2e-11 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 210 - 320 1.5e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 526
Acc36I ACCTGC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 754
AccB7I CCANNNNNTGG 1 cut(s) 151
AclWI GGATC 3 cut(s) 66, 539, 849
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 302
AfiI CCNNNNNNNGG 3 cut(s) 151, 305, 764
AgsI TTSAA 1 cut(s) 874
AjnI CCWGG 3 cut(s) 89, 132, 264
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 6 cut(s) 188, 386, 542, 634, 676, 697
AluI AGCT 6 cut(s) 188, 386, 542, 634, 676, 697
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 2 cut(s) 116, 898
AlwI GGATC 3 cut(s) 66, 539, 849
AlwNI CAGNNNCTG 1 cut(s) 634
Ama87I CYCGRG 1 cut(s) 526
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 24, 862
ApoI RAATTY 1 cut(s) 302
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 3 cut(s) 269, 378, 822
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 5 cut(s) 232, 254, 536, 550, 1000
AvaI CYCGRG 1 cut(s) 526
AvaII GGWCC 3 cut(s) 269, 378, 822
BaeGI GKGCMC 1 cut(s) 757
BalI TGGCCA 1 cut(s) 129
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 3 cut(s) 295, 436, 577
Bbv12I GWGCWC 1 cut(s) 598
BbvI GCAGC 2 cut(s) 36, 849
BccI CCATC 7 cut(s) 329, 470, 503, 797, 830, 926, 965
BciT130I CCWGG 3 cut(s) 91, 134, 266
BciVI GTATCC 1 cut(s) 514
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 2 cut(s) 116, 898
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 271
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 2 cut(s) 25, 863
BlsI GCNGC 2 cut(s) 26, 864
Bme1390I CCNGG 4 cut(s) 91, 134, 266, 591
Bme18I GGWCC 3 cut(s) 269, 378, 822
BmeT110I CYCGRG 1 cut(s) 526
BmgT120I GGNCC 3 cut(s) 269, 378, 822
BmiI GGNNCC 5 cut(s) 270, 380, 435, 576, 756
BmrFI CCNGG 4 cut(s) 91, 134, 266, 591
BmrI ACTGGG 1 cut(s) 542
BmsI GCATC 1 cut(s) 250
BmuI ACTGGG 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 112
BpuMI CCSGG 1 cut(s) 591
Bsa29I ATCGAT 1 cut(s) 534
BsaBI GATNNNNATC 2 cut(s) 76, 840
BsaI GGTCTC 1 cut(s) 898
BsaJI CCNNGG 4 cut(s) 89, 265, 589, 825
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 305, 764
Bse1I ACTGG 6 cut(s) 130, 262, 272, 548, 789, 913
Bse3DI GCAATG 1 cut(s) 783
Bse8I GATNNNNATC 2 cut(s) 76, 840
BseBI CCWGG 3 cut(s) 91, 134, 266
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 4 cut(s) 89, 265, 589, 825
BseGI GGATG 6 cut(s) 191, 230, 295, 748, 808, 841
BseJI GATNNNNATC 2 cut(s) 76, 840
BseLI CCNNNNNNNGG 3 cut(s) 151, 305, 764
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 2 cut(s) 251, 501
BseNI ACTGG 6 cut(s) 130, 262, 272, 548, 789, 913
BseRI GAGGAG 1 cut(s) 426
BseSI GKGCMC 1 cut(s) 757
BseXI GCAGC 2 cut(s) 36, 849
BseYI CCCAGC 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 93
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 754
BshVI ATCGAT 1 cut(s) 534
BsiHKAI GWGCWC 1 cut(s) 598
BsiHKCI CYCGRG 1 cut(s) 526
BsiSI CCGG 2 cut(s) 591, 710
BslFI GGGAC 2 cut(s) 282, 780
BslI CCNNNNNNNGG 3 cut(s) 151, 305, 764
BsmAI GTCTC 2 cut(s) 116, 898
BsmFI GGGAC 2 cut(s) 282, 780
BsnI GGCC 1 cut(s) 129
Bso31I GGTCTC 1 cut(s) 898
BsoBI CYCGRG 1 cut(s) 526
Bsp1286I GDGCHC 5 cut(s) 295, 436, 577, 598, 757
Bsp143I GATC 5 cut(s) 71, 400, 531, 535, 841
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 2 cut(s) 250, 500
BspDI ATCGAT 1 cut(s) 534
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 5 cut(s) 270, 380, 435, 576, 756
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 3 cut(s) 66, 539, 849
BspQI GCTCTTC 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 754
BspTNI GGTCTC 1 cut(s) 898
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 6 cut(s) 130, 262, 272, 548, 789, 913
BssECI CCNNGG 4 cut(s) 89, 265, 589, 825
BssMI GATC 5 cut(s) 71, 400, 531, 535, 841
Bst2UI CCWGG 3 cut(s) 91, 134, 266
Bst4CI ACNGT 5 cut(s) 142, 351, 661, 826, 997
Bst6I CTCTTC 1 cut(s) 720
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 2 cut(s) 237, 487
BstDSI CCRYGG 1 cut(s) 825
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 6 cut(s) 191, 230, 295, 748, 808, 841
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 5 cut(s) 74, 403, 534, 538, 844
BstMAI GTCTC 2 cut(s) 116, 898
BstMBI GATC 5 cut(s) 71, 400, 531, 535, 841
BstMWI GCNNNNNNNGC 1 cut(s) 593
BstNI CCWGG 3 cut(s) 91, 134, 266
BstNSI RCATGY 2 cut(s) 465, 728
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 4 cut(s) 89, 132, 264, 589
BstSLI GKGCMC 1 cut(s) 757
BstV1I GCAGC 2 cut(s) 36, 849
Bsu15I ATCGAT 1 cut(s) 534
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 825
BtsCI GGATG 6 cut(s) 191, 230, 295, 748, 808, 841
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 3 cut(s) 495, 657, 796
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 108
CaiI CAGNNNCTG 1 cut(s) 634
CciI TCATGA 1 cut(s) 439
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 3 cut(s) 269, 378, 822
ClaI ATCGAT 1 cut(s) 534
CviAII CATG 8 cut(s) 146, 157, 389, 440, 462, 725, 866, 897
DdeI CTNAG 2 cut(s) 237, 487
DpnI GATC 5 cut(s) 73, 402, 533, 537, 843
DpnII GATC 5 cut(s) 71, 400, 531, 535, 841
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Eco24I GRGCYC 3 cut(s) 295, 436, 577
Eco31I GGTCTC 1 cut(s) 898
Eco47I GGWCC 3 cut(s) 269, 378, 822
Eco88I CYCGRG 1 cut(s) 526
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 3 cut(s) 89, 132, 264
EcoT22I ATGCAT 1 cut(s) 726
EcoT38I GRGCYC 3 cut(s) 295, 436, 577
FaeI CATG 8 cut(s) 149, 160, 392, 443, 465, 728, 869, 900
FaqI GGGAC 2 cut(s) 282, 780
FatI CATG 8 cut(s) 145, 156, 388, 439, 461, 724, 865, 896
FauNDI CATATG 1 cut(s) 720
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 2 cut(s) 25, 863
FokI GGATG 6 cut(s) 178, 217, 282, 755, 815, 848
FriOI GRGCYC 3 cut(s) 295, 436, 577
Fsp4HI GCNGC 2 cut(s) 25, 863
FspBI CTAG 1 cut(s) 245
GlaI GCGC 1 cut(s) 176
GluI GCNGC 2 cut(s) 25, 863
GsaI CCCAGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 1 cut(s) 129
HapII CCGG 2 cut(s) 591, 710
HhaI GCGC 1 cut(s) 177
Hin1II CATG 8 cut(s) 149, 160, 392, 443, 465, 728, 869, 900
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 1 cut(s) 1000
HindII GTYRAC 1 cut(s) 1000
HindIII AAGCTT 1 cut(s) 674
HpaII CCGG 2 cut(s) 591, 710
HphI GGTGA 5 cut(s) 232, 254, 536, 550, 1000
Hpy166II GTNNAC 3 cut(s) 378, 418, 1000
Hpy188I TCNGA 4 cut(s) 196, 369, 475, 508
Hpy188III TCNNGA 3 cut(s) 53, 440, 628
Hpy8I GTNNAC 3 cut(s) 378, 418, 1000
HpyAV CCTTC 4 cut(s) 349, 353, 845, 952
HpyCH4III ACNGT 5 cut(s) 142, 351, 661, 826, 997
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 110, 500, 724, 865, 878
HpyF10VI GCNNNNNNNGC 1 cut(s) 593
HpyF3I CTNAG 2 cut(s) 237, 487
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 8 cut(s) 149, 160, 392, 443, 465, 728, 869, 900
HspAI GCGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 5 cut(s) 71, 400, 531, 535, 841
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 2 cut(s) 439, 580
Lsp1109I GCAGC 2 cut(s) 36, 849
LweI GCATC 1 cut(s) 250
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 4 cut(s) 260, 556, 946, 988
MalI GATC 5 cut(s) 73, 402, 533, 537, 843
MboI GATC 5 cut(s) 71, 400, 531, 535, 841
MboII GAAGA 2 cut(s) 462, 707
MfeI CAATTG 1 cut(s) 423
MhlI GDGCHC 5 cut(s) 295, 436, 577, 598, 757
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 6 cut(s) 33, 204, 213, 226, 302, 423
MluNI TGGCCA 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 219
Mox20I TGGCCA 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 726
MscI TGGCCA 1 cut(s) 129
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 723
Msp20I TGGCCA 1 cut(s) 129
MspI CCGG 2 cut(s) 591, 710
MspR9I CCNGG 4 cut(s) 91, 134, 266, 591
MunI CAATTG 1 cut(s) 423
MvaI CCWGG 3 cut(s) 91, 134, 266
MwoI GCNNNNNNNGC 1 cut(s) 593
NciI CCSGG 1 cut(s) 591
NdeI CATATG 1 cut(s) 720
NdeII GATC 5 cut(s) 71, 400, 531, 535, 841
NlaIII CATG 8 cut(s) 149, 160, 392, 443, 465, 728, 869, 900
NlaIV GGNNCC 5 cut(s) 270, 380, 435, 576, 756
NmuCI GTSAC 4 cut(s) 260, 556, 946, 988
NsiI ATGCAT 1 cut(s) 726
NspI RCATGY 2 cut(s) 465, 728
PaeR7I CTCGAG 1 cut(s) 526
PagI TCATGA 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 720
PflFI GACNNNGTC 2 cut(s) 140, 623
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 2 cut(s) 26, 864
Psp6I CCWGG 3 cut(s) 89, 132, 264
PspEI GGTNACC 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 516
PspGI CCWGG 3 cut(s) 89, 132, 264
PspN4I GGNNCC 5 cut(s) 270, 380, 435, 576, 756
PspPI GGNCC 3 cut(s) 269, 378, 822
PspXI VCTCGAGB 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 634
PsyI GACNNNGTC 2 cut(s) 140, 623
RseI CAYNNNNRTG 1 cut(s) 723
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 2 cut(s) 25, 863
Sau3AI GATC 5 cut(s) 71, 400, 531, 535, 841
Sau96I GGNCC 3 cut(s) 269, 378, 822
ScrFI CCNGG 4 cut(s) 91, 134, 266, 591
SduI GDGCHC 5 cut(s) 295, 436, 577, 598, 757
SfaNI GCATC 1 cut(s) 250
Sfr274I CTCGAG 1 cut(s) 526
SinI GGWCC 3 cut(s) 269, 378, 822
SlaI CTCGAG 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 1 cut(s) 526
SmoI CTYRAG 1 cut(s) 526
Sse9I AATT 6 cut(s) 33, 204, 213, 226, 302, 423
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 4 cut(s) 89, 132, 264, 589
TaaI ACNGT 5 cut(s) 142, 351, 661, 826, 997
TaiI ACGT 1 cut(s) 65
TaqI TCGA 3 cut(s) 70, 527, 534
TaqII GACCGA 1 cut(s) 437
TasI AATT 6 cut(s) 33, 204, 213, 226, 302, 423
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 3 cut(s) 502, 664, 796
TseFI GTSAC 4 cut(s) 260, 556, 946, 988
TseI GCWGC 2 cut(s) 24, 862
Tsp45I GTSAC 4 cut(s) 260, 556, 946, 988
TspDTI ATGAA 4 cut(s) 180, 219, 456, 707
TspRI CASTG 3 cut(s) 502, 664, 796
Tth111I GACNNNGTC 2 cut(s) 140, 623
Van91I CCANNNNNTGG 1 cut(s) 151
VpaK11BI GGWCC 3 cut(s) 269, 378, 822
XapI RAATTY 1 cut(s) 302
XceI RCATGY 2 cut(s) 465, 728
XhoI CTCGAG 1 cut(s) 526
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.