pycom09g12210

Belongs to the WD repeat SEC13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001675
Physical Location & Seq
Forward (+)
10667608 .. 10668513
906 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12210.1

Sequence Viewer

Length: 906 bp
ATGCCTGCACAAAAGGTTGAGACTGGCCACCAGGACACAGTCCATGATGTGGTCATGGATTACTATGGTAAGCGCGTCGCCACAGCTTCATCCGACAACACAATTAAGATAATTGGGGTGAGCAATTCATCCTCTCAGCATCTAGCAACCCTGACTGGTCACCAGGGACCAGTTTGGCAGGTTGCTTGGGCTCATCCAAAATTCGGGTCTTTACTTGCTTCCTGTTCTTATGATGGGCGTGTCATACTGTGGAAGGAAGGTAATCAGAATGAGTGGACCCAAGCTCATGTTTTTGATGACCACAAATCCTCTGTGAACTCAATTGCTTGGGCTCCTCATGAACTCGGTCTTTGTTTGGCGTGTGGTTCTTCTGATGGGAACATCTCAGTCTTCACTGCAAGGTCTGATGGTGGCTGGGATACCTCGAGGATCGATCAAGCTCACCCAATTGGTGTCACCTCTGTTTCTTGGGCTCCCTCAACAGCCCCCGGTGCTCTTGTTGGTTCTGGTCTGCTTGACCCTGTCCAGAAGCTGTGTTCTGGTGGTTGTGATAATACTGTAAAAGTGTGGAAGCTTGGTAATGGCATTTGGAAGCTGGACTGCTTTCCGGCTCTTCATATGCATGTTGATTGGGTCAGGGATGTTGCTTGGGCACCCAACTTGGGACTACCAAAATCAACCATTGCCAGTGCCTCACAGGATGGTAAAGTGATTATATGGACTGTGGGCAAGGATGGGGATCAATGGGAAGGCAAGGTGCTGCATGATTTCAATGCACCTGTTTGGAGGGTCTCATGGTCGCTGACTGGAAACATATTGGCTGTGGCTGATGGGAACAACAGTGTGACATTATGGAAGGAAGCAGTAGATGGGGAATGGCAACAGGTGACAACAGTTGACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0002376 GO:0002474 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006900 GO:0006901 GO:0006903 GO:0006996 GO:0008104 GO:0008150 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019882 GO:0019884 GO:0019886 GO:0022607 GO:0023051 GO:0023056 GO:0030117 GO:0030120 GO:0030127 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031080 GO:0031090 GO:0031410 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031982 GO:0031984 GO:0032006 GO:0032008 GO:0032991 GO:0033036 GO:0034613 GO:0034622 GO:0035459 GO:0035859 GO:0042175 GO:0042802 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048002 GO:0048193 GO:0048194 GO:0048199 GO:0048207 GO:0048208 GO:0048475 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0051668 GO:0061024 GO:0061700 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0090110 GO:0090114 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098827 GO:0098852 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko03013 ko04141 ko04150 map03013 map04141 map04150
Pfam Domains
Protein Families
WD40

Protein Analysis

302

Amino Acids

32.5

Weight (kDa)

5.66

Isoelectric Point (pI)

25.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 4 - 190 3.1e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 8 - 158 2.2e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 131 3.1e-13 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 8 - 94 3.7e-11 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 9 - 159 2.5e-13 CDC20/Fizzy WD40 domain
WDR55 PF24796 9 - 285 2.8e-06 WDR55
Beta-prop_THOC3 PF25174 10 - 159 9.2e-18 THOC3 beta-propeller domain
WD40_Prp19 PF24814 22 - 159 4.9e-13 Prp19 WD40 domain
WD40_Gbeta PF25391 25 - 160 6.3e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR35_TULP_N PF24797 43 - 159 1.6e-07 WDR35/TULP4 N-terminal
WD40 PF00400 46 - 84 1.5e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 49 - 135 9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 93 - 286 4.1e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 176 - 286 9.4e-12 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 176 - 286 1.4e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 424
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 652
AccB7I CCANNNNNTGG 1 cut(s) 49
AccII CGCG 1 cut(s) 75
AclWI GGATC 2 cut(s) 437, 747
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 1 cut(s) 200
AfiI CCNNNNNNNGG 3 cut(s) 49, 203, 662
AgsI TTSAA 1 cut(s) 772
AjnI CCWGG 2 cut(s) 30, 162
AluBI AGCT 6 cut(s) 86, 284, 440, 532, 574, 595
AluI AGCT 6 cut(s) 86, 284, 440, 532, 574, 595
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 2 cut(s) 14, 796
AlwI GGATC 2 cut(s) 437, 747
AlwNI CAGNNNCTG 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 424
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 1 cut(s) 760
ApoI RAATTY 1 cut(s) 200
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 167, 276
AsuC2I CCSGG 1 cut(s) 489
AsuHPI GGTGA 5 cut(s) 130, 152, 434, 448, 898
AvaI CYCGRG 1 cut(s) 424
AvaII GGWCC 2 cut(s) 167, 276
BaeGI GKGCMC 1 cut(s) 655
BalI TGGCCA 1 cut(s) 27
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 3 cut(s) 193, 334, 475
BbsI GAAGAC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 1 cut(s) 747
BccI CCATC 7 cut(s) 227, 368, 401, 695, 728, 824, 863
BciT130I CCWGG 2 cut(s) 32, 164
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 2 cut(s) 14, 796
BfaI CTAG 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 169
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 1 cut(s) 761
BlsI GCNGC 1 cut(s) 762
Bme1390I CCNGG 3 cut(s) 32, 164, 489
Bme18I GGWCC 2 cut(s) 167, 276
BmeT110I CYCGRG 1 cut(s) 424
BmgT120I GGNCC 2 cut(s) 167, 276
BmiI GGNNCC 5 cut(s) 168, 278, 333, 474, 654
BmrFI CCNGG 3 cut(s) 32, 164, 489
BmsI GCATC 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 489
Bsa29I ATCGAT 1 cut(s) 432
BsaBI GATNNNNATC 1 cut(s) 738
BsaI GGTCTC 1 cut(s) 796
BsaJI CCNNGG 2 cut(s) 163, 487
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 203, 662
Bse1I ACTGG 5 cut(s) 28, 160, 170, 687, 811
Bse3DI GCAATG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 2 cut(s) 32, 164
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 2 cut(s) 163, 487
BseGI GGATG 6 cut(s) 89, 128, 193, 646, 706, 739
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 3 cut(s) 49, 203, 662
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 2 cut(s) 149, 399
BseNI ACTGG 5 cut(s) 28, 160, 170, 687, 811
BseRI GAGGAG 1 cut(s) 324
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 1 cut(s) 747
BseYI CCCAGC 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 27
BshNI GGYRCC 1 cut(s) 652
BshVI ATCGAT 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 496
BsiHKCI CYCGRG 1 cut(s) 424
BsiSI CCGG 2 cut(s) 489, 608
BslFI GGGAC 2 cut(s) 180, 678
BslI CCNNNNNNNGG 3 cut(s) 49, 203, 662
BsmAI GTCTC 2 cut(s) 14, 796
BsmFI GGGAC 2 cut(s) 180, 678
BsnI GGCC 1 cut(s) 27
Bso31I GGTCTC 1 cut(s) 796
BsoBI CYCGRG 1 cut(s) 424
Bsp1286I GDGCHC 5 cut(s) 193, 334, 475, 496, 655
Bsp143I GATC 3 cut(s) 429, 433, 739
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 2 cut(s) 148, 398
BspDI ATCGAT 1 cut(s) 432
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 5 cut(s) 168, 278, 333, 474, 654
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 2 cut(s) 437, 747
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 1 cut(s) 652
BspTNI GGTCTC 1 cut(s) 796
BsrDI GCAATG 1 cut(s) 681
BsrI ACTGG 5 cut(s) 28, 160, 170, 687, 811
BssECI CCNNGG 2 cut(s) 163, 487
BssMI GATC 3 cut(s) 429, 433, 739
Bst2UI CCWGG 2 cut(s) 32, 164
Bst4CI ACNGT 6 cut(s) 40, 249, 559, 724, 842, 895
Bst6I CTCTTC 1 cut(s) 618
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 135, 385
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 6 cut(s) 89, 128, 193, 646, 706, 739
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 3 cut(s) 432, 436, 742
BstMAI GTCTC 2 cut(s) 14, 796
BstMBI GATC 3 cut(s) 429, 433, 739
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstNI CCWGG 2 cut(s) 32, 164
BstNSI RCATGY 1 cut(s) 626
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 3 cut(s) 30, 162, 487
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 747
BstV2I GAAGAC 1 cut(s) 382
Bsu15I ATCGAT 1 cut(s) 432
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 1 cut(s) 27
BsuTUI ATCGAT 1 cut(s) 432
BtsCI GGATG 6 cut(s) 89, 128, 193, 646, 706, 739
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 3 cut(s) 393, 694, 847
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 532
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 167, 276
ClaI ATCGAT 1 cut(s) 432
CseI GACGC 1 cut(s) 64
CviAII CATG 7 cut(s) 44, 55, 287, 338, 623, 764, 795
DdeI CTNAG 2 cut(s) 135, 385
DpnI GATC 3 cut(s) 431, 435, 741
DpnII GATC 3 cut(s) 429, 433, 739
EaeI YGGCCR 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco24I GRGCYC 3 cut(s) 193, 334, 475
Eco31I GGTCTC 1 cut(s) 796
Eco47I GGWCC 2 cut(s) 167, 276
Eco88I CYCGRG 1 cut(s) 424
Eco91I GGTNACC 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 30, 162
EcoT22I ATGCAT 1 cut(s) 624
EcoT38I GRGCYC 3 cut(s) 193, 334, 475
FaeI CATG 7 cut(s) 47, 58, 290, 341, 626, 767, 798
FaqI GGGAC 2 cut(s) 180, 678
FatI CATG 7 cut(s) 43, 54, 286, 337, 622, 763, 794
FauNDI CATATG 1 cut(s) 618
Fnu4HI GCNGC 1 cut(s) 761
FokI GGATG 6 cut(s) 76, 115, 180, 653, 713, 746
FriOI GRGCYC 3 cut(s) 193, 334, 475
Fsp4HI GCNGC 1 cut(s) 761
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 74
GluI GCNGC 1 cut(s) 761
GsaI CCCAGC 1 cut(s) 418
HaeIII GGCC 1 cut(s) 27
HapII CCGG 2 cut(s) 489, 608
HgaI GACGC 1 cut(s) 64
HhaI GCGC 1 cut(s) 75
Hin1II CATG 7 cut(s) 47, 58, 290, 341, 626, 767, 798
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HindIII AAGCTT 1 cut(s) 572
HpaII CCGG 2 cut(s) 489, 608
HphI GGTGA 5 cut(s) 130, 152, 434, 448, 898
Hpy166II GTNNAC 3 cut(s) 276, 316, 898
Hpy188I TCNGA 4 cut(s) 94, 267, 373, 406
Hpy188III TCNNGA 2 cut(s) 338, 526
Hpy8I GTNNAC 3 cut(s) 276, 316, 898
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 4 cut(s) 247, 251, 743, 850
HpyCH4III ACNGT 6 cut(s) 40, 249, 559, 724, 842, 895
HpyCH4V TGCA 5 cut(s) 8, 398, 622, 763, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
HpyF3I CTNAG 2 cut(s) 135, 385
Hsp92II CATG 7 cut(s) 47, 58, 290, 341, 626, 767, 798
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 3 cut(s) 429, 433, 739
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 2 cut(s) 337, 478
Lsp1109I GCAGC 1 cut(s) 747
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 143
MaeIII GTNAC 4 cut(s) 158, 454, 844, 886
MalI GATC 3 cut(s) 431, 435, 741
MboI GATC 3 cut(s) 429, 433, 739
MboII GAAGA 3 cut(s) 360, 382, 605
MfeI CAATTG 2 cut(s) 321, 447
MhlI GDGCHC 5 cut(s) 193, 334, 475, 496, 655
MlsI TGGCCA 1 cut(s) 27
MluCI AATT 6 cut(s) 102, 111, 124, 200, 321, 447
MluNI TGGCCA 1 cut(s) 27
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 9 cut(s) 142, 319, 345, 420, 433, 469, 487, 703, 780
Mox20I TGGCCA 1 cut(s) 27
Mph1103I ATGCAT 1 cut(s) 624
MscI TGGCCA 1 cut(s) 27
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 1 cut(s) 621
Msp20I TGGCCA 1 cut(s) 27
MspI CCGG 2 cut(s) 489, 608
MspR9I CCNGG 3 cut(s) 32, 164, 489
MunI CAATTG 2 cut(s) 321, 447
MvaI CCWGG 2 cut(s) 32, 164
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 491
NciI CCSGG 1 cut(s) 489
NdeI CATATG 1 cut(s) 618
NdeII GATC 3 cut(s) 429, 433, 739
NlaIII CATG 7 cut(s) 47, 58, 290, 341, 626, 767, 798
NlaIV GGNNCC 5 cut(s) 168, 278, 333, 474, 654
NmuCI GTSAC 4 cut(s) 158, 454, 844, 886
NsiI ATGCAT 1 cut(s) 624
NspI RCATGY 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 424
PagI TCATGA 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 618
PflFI GACNNNGTC 2 cut(s) 38, 521
PflMI CCANNNNNTGG 1 cut(s) 49
PkrI GCNGC 1 cut(s) 762
Psp6I CCWGG 2 cut(s) 30, 162
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 2 cut(s) 30, 162
PspN4I GGNNCC 5 cut(s) 168, 278, 333, 474, 654
PspPI GGNCC 2 cut(s) 167, 276
PspXI VCTCGAGB 1 cut(s) 424
PstNI CAGNNNCTG 1 cut(s) 532
PsyI GACNNNGTC 2 cut(s) 38, 521
RseI CAYNNNNRTG 1 cut(s) 621
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 1 cut(s) 761
Sau3AI GATC 3 cut(s) 429, 433, 739
Sau96I GGNCC 2 cut(s) 167, 276
ScrFI CCNGG 3 cut(s) 32, 164, 489
SduI GDGCHC 5 cut(s) 193, 334, 475, 496, 655
SfaNI GCATC 1 cut(s) 148
Sfr274I CTCGAG 1 cut(s) 424
SinI GGWCC 2 cut(s) 167, 276
SlaI CTCGAG 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
Sse9I AATT 6 cut(s) 102, 111, 124, 200, 321, 447
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 3 cut(s) 30, 162, 487
TaaI ACNGT 6 cut(s) 40, 249, 559, 724, 842, 895
TaqI TCGA 2 cut(s) 425, 432
TaqII GACCGA 1 cut(s) 335
TasI AATT 6 cut(s) 102, 111, 124, 200, 321, 447
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 3 cut(s) 400, 694, 847
TseFI GTSAC 4 cut(s) 158, 454, 844, 886
TseI GCWGC 1 cut(s) 760
Tsp45I GTSAC 4 cut(s) 158, 454, 844, 886
TspDTI ATGAA 4 cut(s) 78, 117, 354, 605
TspRI CASTG 3 cut(s) 400, 694, 847
Tth111I GACNNNGTC 2 cut(s) 38, 521
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 2 cut(s) 167, 276
XapI RAATTY 1 cut(s) 200
XceI RCATGY 1 cut(s) 626
XhoI CTCGAG 1 cut(s) 424
XspI CTAG 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.