Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001675

Physical Location & Seq

Genomic Coordinates Reverse (-)

37251705 .. 37253452

Gene Structure 1748 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g49840.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 909 bp

ATGCCGGGGCAGAAGATTGAAACGGGTCACCAGGATGTTGTTCATGATGTTGCCATGGATTACTATGGGAAACGTCTAGCATCAGCTTCATCAGATACCACTATTAAGATAATTGGTGTCGGAAGCAATGCGAGTTCACAGCACCTTGCTACGTTGTCAGGTCACAGAGGTCCTGTGTGGCAGGTGGGTTGGGCGCATCCCAAGTTTGGGCCGATCCTTGCTTCGTGCTCGTATGATGGCCAAGTGATAATTTGGAAGGAGGGTAATCCAAATGAGTGGTACCAGGCTCATGTTTTCAATGAGCATAGTGCGTCTGTGAATTCCATTTGTTGGGCGCCTCATGAACTTGGACTCATCTTAGCTTGTGGTTCTTCTGATGGGACCATCTCGGTTTGCACTGCTAGGGCAGATGGTGGTTGGGACACCACTAGGATAGACCAAGCAGACCTTGTTGGAGTAACCTCCGTTTCGTGGGCTCCATCAATGGCTCCTGGTTCTTTAGTTGGAACTGGCATGCTTGAACCTGTTCAGAAGCTGGTCTCTGGTGGCTGCGACAATACAGTGAAGGTGTGGAAATTGTACAATGGGACTTGGAAGATGGATTGCTTCCCCGCTCTTCAAATGCACACCGATTGGGTTAGAGATGTTGCCTGGGCACCCAACTTGGGACTTCCTAAGTCCACCATTGCAAGTTGTTCACAGGATGGAACAGTAATTATCTGGACTGTGGCCAAAGAAGGCGATCAATGGCAGGGTAAGGTTTTAAATGATTTTAAGACCCCGGTTTGGAGGGTGTCATGGTCACTAACAGGAAACCTACTGGCCGTGGCTGATGGAAATAACGAAGTAACATTGTGGAAAGAGTCTGTTGATGGCGAGTGGCAACAAGTGAGCACAGTCGATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005829 GO:0006810 GO:0006888 GO:0008150 GO:0012505 GO:0016020 GO:0016192 GO:0031080 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0098588 GO:0098805 GO:0098852

KEGG Pathways

Metabolic & Signaling

ko03013 ko04141 ko04150 map03013 map04141 map04150

Pfam Domains

Protein Families

WD40

Protein Analysis

303

Amino Acids

32.69

Weight (kDa)

5.27

Isoelectric Point (pI)

24.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	8 - 136	7.2e-15	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	8 - 86	6.9e-11	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	9 - 137	4e-12	CDC20/Fizzy WD40 domain
WDR55	PF24796	9 - 287	3.3e-06	WDR55
Beta-prop_THOC3	PF25174	10 - 160	1.6e-17	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	11 - 114	1.8e-10	WDHD1 first WD40 domain
Beta-prop_WDR35_TULP_N	PF24797	41 - 161	5.1e-06	WDR35/TULP4 N-terminal
WD40_Prp19	PF24814	42 - 172	1.5e-09	Prp19 WD40 domain
WD40	PF00400	47 - 85	4.1e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	174 - 247	5.7e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	177 - 287	2e-14	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	177 - 287	8.8e-13	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	177 - 287	1.1e-09	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	177 - 289	2.1e-08	WDHD1 first WD40 domain
WD40_Prp19	PF24814	177 - 286	1.8e-07	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001675)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30050 AT3G01340 AT3G01340
fragaria_vesca	FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g49840 FvH4_6g49840 FvH4_6g49840
malus_domestica	MD09G1041000.v1.1 MD09G1205500.v1.1 MD17G1042300.v1.1 MD17G1185700.v1.1
prunus_persica	Prupe.3G060800_v2.0.a1 Prupe.3G276700_v2.0.a1
pyrus_communis	pycom09g12210 pycom111g03200 pycom17g03790 pycom17g19510
rosa_chinensis	RchiOBHm_Chr2g0140101 RchiOBHm_Chr2g0170031
rosa_laevigata	RLG00000010169 RLG00000019849 RLG00000021917
rosa_multiflora	Rmu_co8506925.1_g000001 Rmu_sc0003244.1_g000028 Rmu_sc0013078.1_g000015 Rmu_ssc0000389.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081680 Rroxscaffold_2G00105060
rosa_rugosa	Rorug02G0361100 Rorug02G0361200 Rorug02G0545000 Rorug02G0545100
rosa_samantha	Rh2AG411800 Rh2AG617500 Rh2BG422100 Rh2BG628800 Rh2CG397900 Rh2CG598300 Rh2DG431500 Rh2DG640000 Rh2DG640600 Rh4BG007800
rosa_wichuraiana	Rw2G033740 Rw2G051120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	172
Acc36I	ACCTGC	1 cut(s)	172
Acc65I	GGTACC	1 cut(s)	279
AccB1I	GGYRCC	3 cut(s)	279, 334, 655
AccB7I	CCANNNNNTGG	1 cut(s)	330
AccBSI	CCGCTC	1 cut(s)	614
AciI	CCGC	1 cut(s)	612
AclWI	GGATC	1 cut(s)	208
AcoI	YGGCCR	3 cut(s)	238, 729, 822
AcsI	RAATTY	1 cut(s)	319
AcyI	GRCGYC	1 cut(s)	335
AfaI	GTAC	2 cut(s)	281, 581
AfiI	CCNNNNNNNGG	6 cut(s)	206, 207, 330, 471, 665, 786
AgsI	TTSAA	4 cut(s)	20, 298, 521, 620
AjnI	CCWGG	4 cut(s)	30, 282, 490, 650
AluBI	AGCT	3 cut(s)	86, 362, 535
AluI	AGCT	3 cut(s)	86, 362, 535
Alw21I	GWGCWC	2 cut(s)	230, 896
Alw26I	GTCTC	1 cut(s)	544
AlwI	GGATC	1 cut(s)	208
AlwNI	CAGNNNCTG	1 cut(s)	535
AoxI	GGCC	4 cut(s)	209, 238, 729, 822
ApeKI	GCWGC	1 cut(s)	549
ApoI	RAATTY	1 cut(s)	319
Asp700I	GAANNNNTTC	1 cut(s)	525
Asp718I	GGTACC	1 cut(s)	279
AspLEI	GCGC	2 cut(s)	196, 337
AspS9I	GGNCC	3 cut(s)	170, 209, 381
AsuC2I	CCSGG	2 cut(s)	6, 782
AsuHPI	GGTGA	1 cut(s)	20
AvaII	GGWCC	2 cut(s)	170, 381
BaeGI	GKGCMC	1 cut(s)	658
BalI	TGGCCA	2 cut(s)	240, 731
BanI	GGYRCC	3 cut(s)	279, 334, 655
BanII	GRGCYC	1 cut(s)	478
Bbv12I	GWGCWC	2 cut(s)	230, 896
BbvI	GCAGC	1 cut(s)	536
BccI	CCATC	9 cut(s)	230, 371, 392, 404, 487, 592, 698, 827, 866
BceAI	ACGGC	1 cut(s)	809
BciT130I	CCWGG	4 cut(s)	32, 284, 492, 652
BcnI	CCSGG	2 cut(s)	6, 782
BcoDI	GTCTC	1 cut(s)	544
BfaI	CTAG	3 cut(s)	77, 402, 429
BfoI	RGCGCY	1 cut(s)	338
BfuAI	ACCTGC	1 cut(s)	172
BisI	GCNGC	1 cut(s)	550
BlsI	GCNGC	1 cut(s)	551
Bme1390I	CCNGG	6 cut(s)	6, 32, 284, 492, 652, 782
Bme18I	GGWCC	2 cut(s)	170, 381
BmgT120I	GGNCC	3 cut(s)	170, 209, 381
BmiI	GGNNCC	6 cut(s)	281, 336, 382, 477, 489, 657
BmrFI	CCNGG	6 cut(s)	6, 32, 284, 492, 652, 782
BmsI	GCATC	3 cut(s)	89, 205, 892
BpuMI	CCSGG	2 cut(s)	6, 782
BsaHI	GRCGYC	1 cut(s)	335
BsaI	GGTCTC	1 cut(s)	544
BsaJI	CCNNGG	5 cut(s)	5, 54, 651, 780, 825
Bsc4I	CCNNNNNNNGG	6 cut(s)	206, 207, 330, 471, 665, 786
Bse1I	ACTGG	2 cut(s)	514, 825
Bse3DI	GCAATG	2 cut(s)	133, 684
BseBI	CCWGG	4 cut(s)	32, 284, 492, 652
BseDI	CCNNGG	5 cut(s)	5, 54, 651, 780, 825
BseGI	GGATG	3 cut(s)	40, 196, 709
BseLI	CCNNNNNNNGG	6 cut(s)	206, 207, 330, 471, 665, 786
BseMI	GCAATG	2 cut(s)	133, 684
BseNI	ACTGG	2 cut(s)	514, 825
BseSI	GKGCMC	1 cut(s)	658
BseXI	GCAGC	1 cut(s)	536
BshFI	GGCC	4 cut(s)	211, 240, 731, 824
BshNI	GGYRCC	3 cut(s)	279, 334, 655
BsiHKAI	GWGCWC	2 cut(s)	230, 896
BsiSI	CCGG	2 cut(s)	5, 782
BslFI	GGGAC	4 cut(s)	394, 434, 601, 681
BslI	CCNNNNNNNGG	6 cut(s)	206, 207, 330, 471, 665, 786
BsmAI	GTCTC	1 cut(s)	544
BsmFI	GGGAC	4 cut(s)	394, 434, 601, 681
BsnI	GGCC	4 cut(s)	211, 240, 731, 824
Bso31I	GGTCTC	1 cut(s)	544
Bsp1286I	GDGCHC	4 cut(s)	230, 478, 658, 896
Bsp1407I	TGTACA	1 cut(s)	579
Bsp143I	GATC	2 cut(s)	213, 742
Bsp19I	CCATGG	1 cut(s)	54
BspACI	CCGC	1 cut(s)	612
BspANI	GGCC	4 cut(s)	211, 240, 731, 824
BspHI	TCATGA	2 cut(s)	43, 340
BspLI	GGNNCC	6 cut(s)	281, 336, 382, 477, 489, 657
BspMI	ACCTGC	1 cut(s)	172
BspPI	GGATC	1 cut(s)	208
BspQI	GCTCTTC	1 cut(s)	621
BspT107I	GGYRCC	3 cut(s)	279, 334, 655
BspTNI	GGTCTC	1 cut(s)	544
BsrBI	CCGCTC	1 cut(s)	614
BsrDI	GCAATG	2 cut(s)	133, 684
BsrGI	TGTACA	1 cut(s)	579
BsrI	ACTGG	2 cut(s)	514, 825
BssECI	CCNNGG	5 cut(s)	5, 54, 651, 780, 825
BssMI	GATC	2 cut(s)	213, 742
BssNI	GRCGYC	1 cut(s)	335
BssT1I	CCWWGG	1 cut(s)	54
Bst2UI	CCWGG	4 cut(s)	32, 284, 492, 652
Bst4CI	ACNGT	4 cut(s)	562, 712, 727, 898
Bst6I	CTCTTC	1 cut(s)	621
BstACI	GRCGYC	1 cut(s)	335
BstAUI	TGTACA	1 cut(s)	579
BstC8I	GCNNGC	1 cut(s)	515
BstDEI	CTNAG	2 cut(s)	358, 675
BstDSI	CCRYGG	2 cut(s)	54, 825
BstEII	GGTNACC	1 cut(s)	26
BstF5I	GGATG	3 cut(s)	40, 196, 709
BstH2I	RGCGCY	1 cut(s)	338
BstHHI	GCGC	2 cut(s)	196, 337
BstKTI	GATC	2 cut(s)	216, 745
BstMAI	GTCTC	1 cut(s)	544
BstMBI	GATC	2 cut(s)	213, 742
BstNI	CCWGG	4 cut(s)	32, 284, 492, 652
BstNSI	RCATGY	1 cut(s)	517
BstPI	GGTNACC	1 cut(s)	26
BstSCI	CCNGG	6 cut(s)	4, 30, 282, 490, 650, 780
BstSLI	GKGCMC	1 cut(s)	658
BstV1I	GCAGC	1 cut(s)	536
BstXI	CCANNNNNNTGG	1 cut(s)	276
BsuRI	GGCC	4 cut(s)	211, 240, 731, 824
BtgI	CCRYGG	2 cut(s)	54, 825
BtsCI	GGATG	3 cut(s)	40, 196, 709
BtsI	GCAGTG	1 cut(s)	396
BtsIMutI	CAGTG	2 cut(s)	396, 567
BveI	ACCTGC	1 cut(s)	172
Cac8I	GCNNGC	1 cut(s)	515
CaiI	CAGNNNCTG	1 cut(s)	535
CciI	TCATGA	2 cut(s)	43, 340
CfoI	GCGC	2 cut(s)	196, 337
Cfr13I	GGNCC	3 cut(s)	170, 209, 381
CseI	GACGC	1 cut(s)	300
Csp6I	GTAC	2 cut(s)	280, 580
CviAII	CATG	6 cut(s)	44, 55, 290, 341, 514, 798
CviQI	GTAC	2 cut(s)	280, 580
DdeI	CTNAG	2 cut(s)	358, 675
DinI	GGCGCC	1 cut(s)	336
DpnI	GATC	2 cut(s)	215, 744
DpnII	GATC	2 cut(s)	213, 742
DraI	TTTAAA	1 cut(s)	765
EaeI	YGGCCR	3 cut(s)	238, 729, 822
Eam1104I	CTCTTC	1 cut(s)	621
EarI	CTCTTC	1 cut(s)	621
Eco130I	CCWWGG	1 cut(s)	54
Eco24I	GRGCYC	1 cut(s)	478
Eco31I	GGTCTC	1 cut(s)	544
Eco47I	GGWCC	2 cut(s)	170, 381
Eco91I	GGTNACC	1 cut(s)	26
EcoO109I	RGGNCCY	1 cut(s)	170
EcoO65I	GGTNACC	1 cut(s)	26
EcoRI	GAATTC	1 cut(s)	319
EcoRII	CCWGG	4 cut(s)	30, 282, 490, 650
EcoT14I	CCWWGG	1 cut(s)	54
EcoT22I	ATGCAT	1 cut(s)	907
EcoT38I	GRGCYC	1 cut(s)	478
EgeI	GGCGCC	1 cut(s)	336
EheI	GGCGCC	1 cut(s)	336
ErhI	CCWWGG	1 cut(s)	54
FaeI	CATG	6 cut(s)	47, 58, 293, 344, 517, 801
FalI	AAGNNNNNCTT	2 cut(s)	432, 464
FaqI	GGGAC	4 cut(s)	394, 434, 601, 681
FatI	CATG	6 cut(s)	43, 54, 289, 340, 513, 797
FauI	CCCGC	1 cut(s)	619
Fnu4HI	GCNGC	1 cut(s)	550
FokI	GGATG	3 cut(s)	47, 183, 716
FriOI	GRGCYC	1 cut(s)	478
Fsp4HI	GCNGC	1 cut(s)	550
FspBI	CTAG	3 cut(s)	77, 402, 429
GlaI	GCGC	2 cut(s)	195, 336
GluI	GCNGC	1 cut(s)	550
HaeII	RGCGCY	1 cut(s)	338
HaeIII	GGCC	4 cut(s)	211, 240, 731, 824
HapII	CCGG	2 cut(s)	5, 782
HgaI	GACGC	1 cut(s)	300
HhaI	GCGC	2 cut(s)	196, 337
Hin1I	GRCGYC	1 cut(s)	335
Hin1II	CATG	6 cut(s)	47, 58, 293, 344, 517, 801
Hin6I	GCGC	2 cut(s)	194, 335
HinP1I	GCGC	2 cut(s)	194, 335
HinfI	GANTC	2 cut(s)	351, 863
HpaII	CCGG	2 cut(s)	5, 782
HphI	GGTGA	1 cut(s)	20
Hpy166II	GTNNAC	3 cut(s)	137, 681, 698
Hpy188I	TCNGA	4 cut(s)	94, 122, 376, 531
Hpy188III	TCNNGA	3 cut(s)	44, 341, 721
Hpy8I	GTNNAC	3 cut(s)	137, 681, 698
HpyAV	CCTTC	3 cut(s)	250, 559, 731
HpyCH4III	ACNGT	4 cut(s)	562, 712, 727, 898
HpyCH4IV	ACGT	2 cut(s)	73, 152
HpyCH4V	TGCA	4 cut(s)	396, 625, 689, 905
HpyF3I	CTNAG	2 cut(s)	358, 675
HpySE526I	ACGT	2 cut(s)	73, 152
Hsp92I	GRCGYC	1 cut(s)	335
Hsp92II	CATG	6 cut(s)	47, 58, 293, 344, 517, 801
HspAI	GCGC	2 cut(s)	194, 335
KasI	GGCGCC	1 cut(s)	334
KpnI	GGTACC	1 cut(s)	283
Kzo9I	GATC	2 cut(s)	213, 742
LguI	GCTCTTC	1 cut(s)	621
LmnI	GCTCC	2 cut(s)	481, 493
Lsp1109I	GCAGC	1 cut(s)	536
LweI	GCATC	3 cut(s)	89, 205, 892
MaeI	CTAG	3 cut(s)	77, 402, 429
MaeII	ACGT	2 cut(s)	73, 152
MaeIII	GTNAC	5 cut(s)	26, 161, 457, 801, 847
MalI	GATC	2 cut(s)	215, 744
MbiI	CCGCTC	1 cut(s)	614
MboI	GATC	2 cut(s)	213, 742
MboII	GAAGA	4 cut(s)	25, 363, 607, 608
MhlI	GDGCHC	4 cut(s)	230, 478, 658, 896
MlsI	TGGCCA	2 cut(s)	240, 731
MluCI	AATT	5 cut(s)	111, 249, 319, 575, 714
MluNI	TGGCCA	2 cut(s)	240, 731
Mly113I	GGCGCC	1 cut(s)	335
MlyI	GAGTC	2 cut(s)	345, 872
MmeI	TCCRAC	3 cut(s)	100, 433, 484
MnlI	CCTC	5 cut(s)	161, 253, 348, 472, 783
Mox20I	TGGCCA	2 cut(s)	240, 731
Mph1103I	ATGCAT	1 cut(s)	907
MroXI	GAANNNNTTC	1 cut(s)	525
MscI	TGGCCA	2 cut(s)	240, 731
MseI	TTAA	3 cut(s)	105, 764, 774
MslI	CAYNNNNRTG	1 cut(s)	33
Msp20I	TGGCCA	2 cut(s)	240, 731
MspI	CCGG	2 cut(s)	5, 782
MspR9I	CCNGG	6 cut(s)	6, 32, 284, 492, 652, 782
MvaI	CCWGG	4 cut(s)	32, 284, 492, 652
NarI	GGCGCC	1 cut(s)	335
NciI	CCSGG	2 cut(s)	6, 782
NcoI	CCATGG	1 cut(s)	54
NdeII	GATC	2 cut(s)	213, 742
NlaIII	CATG	6 cut(s)	47, 58, 293, 344, 517, 801
NlaIV	GGNNCC	6 cut(s)	281, 336, 382, 477, 489, 657
NmuCI	GTSAC	3 cut(s)	26, 161, 801
NsiI	ATGCAT	1 cut(s)	907
NspI	RCATGY	1 cut(s)	517
PaeI	GCATGC	1 cut(s)	517
PagI	TCATGA	2 cut(s)	43, 340
PaqCI	CACCTGC	1 cut(s)	172
PciSI	GCTCTTC	1 cut(s)	621
PdmI	GAANNNNTTC	1 cut(s)	525
PflMI	CCANNNNNTGG	1 cut(s)	330
PkrI	GCNGC	1 cut(s)	551
PleI	GAGTC	2 cut(s)	345, 871
PluTI	GGCGCC	1 cut(s)	338
PpsI	GAGTC	2 cut(s)	345, 871
PpuMI	RGGWCCY	1 cut(s)	170
Psp5II	RGGWCCY	1 cut(s)	170
Psp6I	CCWGG	4 cut(s)	30, 282, 490, 650
PspEI	GGTNACC	1 cut(s)	26
PspGI	CCWGG	4 cut(s)	30, 282, 490, 650
PspN4I	GGNNCC	6 cut(s)	281, 336, 382, 477, 489, 657
PspPI	GGNCC	3 cut(s)	170, 209, 381
PspPPI	RGGWCCY	1 cut(s)	170
PstNI	CAGNNNCTG	1 cut(s)	535
RsaI	GTAC	2 cut(s)	281, 581
RsaNI	GTAC	2 cut(s)	280, 580
RseI	CAYNNNNRTG	1 cut(s)	33
SapI	GCTCTTC	1 cut(s)	621
SaqAI	TTAA	3 cut(s)	105, 764, 774
SatI	GCNGC	1 cut(s)	550
Sau3AI	GATC	2 cut(s)	213, 742
Sau96I	GGNCC	3 cut(s)	170, 209, 381
SchI	GAGTC	2 cut(s)	345, 872
ScrFI	CCNGG	6 cut(s)	6, 32, 284, 492, 652, 782
SduI	GDGCHC	4 cut(s)	230, 478, 658, 896
SfaNI	GCATC	3 cut(s)	89, 205, 892
SfoI	GGCGCC	1 cut(s)	336
SinI	GGWCC	2 cut(s)	170, 381
SmiMI	CAYNNNNRTG	1 cut(s)	33
SphI	GCATGC	1 cut(s)	517
Sse9I	AATT	5 cut(s)	111, 249, 319, 575, 714
SsiI	CCGC	1 cut(s)	612
SspDI	GGCGCC	1 cut(s)	334
SspMI	CTAG	3 cut(s)	77, 402, 429
StyD4I	CCNGG	6 cut(s)	4, 30, 282, 490, 650, 780
StyI	CCWWGG	1 cut(s)	54
TaaI	ACNGT	4 cut(s)	562, 712, 727, 898
TaiI	ACGT	2 cut(s)	76, 155
TaqI	TCGA	1 cut(s)	900
TasI	AATT	5 cut(s)	111, 249, 319, 575, 714
TatI	WGTACW	1 cut(s)	579
Tru1I	TTAA	3 cut(s)	105, 764, 774
Tru9I	TTAA	3 cut(s)	105, 764, 774
TscAI	CASTG	2 cut(s)	403, 567
TseFI	GTSAC	3 cut(s)	26, 161, 801
TseI	GCWGC	1 cut(s)	549
Tsp45I	GTSAC	3 cut(s)	26, 161, 801
TspDTI	ATGAA	3 cut(s)	32, 78, 357
TspGWI	ACGGA	1 cut(s)	454
TspRI	CASTG	2 cut(s)	403, 567
Van91I	CCANNNNNTGG	1 cut(s)	330
VpaK11BI	GGWCC	2 cut(s)	170, 381
XapI	RAATTY	1 cut(s)	319
XceI	RCATGY	1 cut(s)	517
XmnI	GAANNNNTTC	1 cut(s)	525
XspI	CTAG	3 cut(s)	77, 402, 429
Zsp2I	ATGCAT	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.