Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001675

Physical Location & Seq

Genomic Coordinates Forward (+)

2640031 .. 2641626

Gene Structure 1596 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1041000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGCCTGCACAGAAGATCGAAACGGGTCACCAGGACACTGTCCACGATGTAGCCATGGATTACTACGGAAAGCGTCTAGCAACAGCTTCGTCTGATGCCACCATTAAGATAATTGGTGTTGGAAACGCATCACAGCACCTAGCTACATTGTCAGCTCATAGCGGCCCTGTTTGGGAGGTAGCTTGGGCGCATCCCAAGTTCGGGTCGATCCTTGCTTCCTGTTCTTACGATGGTCAGGTGATCATCTGGAAGGAGGGTAATCCGAATGAGTGGCAACAGGCTCATGTTTTTAATGACCACAAGTCGTCAGTAAATTCCATTTCTTGGGCGCCTCATGAACTTGGTCTAATCTTAGCTTGCGGTTCTTCTGATGGGAATATATCGGTTTTCACTGCCAGGGCCGATGGTGGTTGGGACACCACTAAGATAGATCAAGCCCACCCTGTTGGAGTAACCTCAGTTTCATGGGCCCCAGCAATGGCTCCTGGCGCTCTAGTAGGATCTGGTCTGCTTGATCCCGTTCATAAGCTGGCATCCGGTGGGTGCGATAATACTGTGAAGGTGTGGAAGCTGTACAATGGGATTTGGAAGATGGATTGCTTCCCCGCCCTTCAAATGCACAGTGATTGGGTGAGAGATGTAGCCTGGGCACCCAACTTGGGACTGCCAAAGTCCACAATCGCAAGTGCTTCGCAGGATGGAACAGTGGTGATTTGGATTGTGGCTAAGGAAGGCGAGCAATGGGAAGGGAAGGTTTTGAAGGATTTCAAGACCCCGGTCTGGAGGGTGTCATGGTCACTAACCGGAAATTTGCTGGCGGTAGCTGATGGGGATAACAATGTAACATTGTGGAAAGAATCTGTCGATGGCGAGTGGCAACAAGTAAGCACAGTCGAGCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005829 GO:0006810 GO:0006888 GO:0008150 GO:0012505 GO:0016020 GO:0016192 GO:0031080 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0098588 GO:0098805 GO:0098852

KEGG Pathways

Metabolic & Signaling

ko03013 ko04141 ko04150 map03013 map04141 map04150

Pfam Domains

Protein Families

WD40

Protein Analysis

301

Amino Acids

32.42

Weight (kDa)

5.48

Isoelectric Point (pI)

28.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_TEP1_2nd	PF25047	4 - 131	1.1e-06	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	5 - 112	6.5e-12	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	8 - 157	5.3e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	8 - 84	9.1e-10	WDR3 first beta-propeller domain
WDR55	PF24796	9 - 133	2.2e-06	WDR55
WD40_CDC20-Fz	PF24807	9 - 142	8.2e-14	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	10 - 158	1.2e-16	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	10 - 134	4.8e-06	MABP1/WDR62 second WD40 domain
WD40_Prp19	PF24814	14 - 169	2.1e-12	Prp19 WD40 domain
WD40	PF00400	45 - 83	2e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	48 - 133	4.5e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2	PF23414	68 - 157	7.3e-06	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	92 - 285	9.4e-13	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	117 - 242	5.4e-07	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	173 - 246	1.3e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	175 - 285	4.8e-15	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	176 - 287	6.7e-07	WDHD1 first WD40 domain
WD40_Prp19	PF24814	176 - 285	2.1e-07	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	176 - 285	6.1e-14	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	176 - 286	8.6e-06	TEP-1 second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001675)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30050 AT3G01340 AT3G01340
fragaria_vesca	FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g32220 FvH4_6g49840 FvH4_6g49840 FvH4_6g49840
malus_domestica	MD09G1041000.v1.1 MD09G1205500.v1.1 MD17G1042300.v1.1 MD17G1185700.v1.1
prunus_persica	Prupe.3G060800_v2.0.a1 Prupe.3G276700_v2.0.a1
pyrus_communis	pycom09g12210 pycom111g03200 pycom17g03790 pycom17g19510
rosa_chinensis	RchiOBHm_Chr2g0140101 RchiOBHm_Chr2g0170031
rosa_laevigata	RLG00000010169 RLG00000019849 RLG00000021917
rosa_multiflora	Rmu_co8506925.1_g000001 Rmu_sc0003244.1_g000028 Rmu_sc0013078.1_g000015 Rmu_ssc0000389.1_g000004
rosa_roxburghii	Rroxscaffold_2G00081680 Rroxscaffold_2G00105060
rosa_rugosa	Rorug02G0361100 Rorug02G0361200 Rorug02G0545000 Rorug02G0545100
rosa_samantha	Rh2AG411800 Rh2AG617500 Rh2BG422100 Rh2BG628800 Rh2CG397900 Rh2CG598300 Rh2DG431500 Rh2DG640000 Rh2DG640600 Rh4BG007800
rosa_wichuraiana	Rw2G033740 Rw2G051120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	328, 649
AccB7I	CCANNNNNTGG	1 cut(s)	324
AciI	CCGC	4 cut(s)	162, 360, 606, 818
AclWI	GGATC	3 cut(s)	202, 508, 509
AcsI	RAATTY	2 cut(s)	313, 808
AcyI	GRCGYC	1 cut(s)	329
AfaI	GTAC	1 cut(s)	575
AfiI	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 324, 478, 659, 780
AgsI	TTSAA	3 cut(s)	614, 760, 769
AhdI	GACNNNNNGTC	1 cut(s)	301
AjnI	CCWGG	4 cut(s)	30, 395, 484, 644
AluBI	AGCT	8 cut(s)	86, 143, 155, 182, 356, 529, 571, 824
AluI	AGCT	8 cut(s)	86, 143, 155, 182, 356, 529, 571, 824
AlwI	GGATC	3 cut(s)	202, 508, 509
AoxI	GGCC	3 cut(s)	163, 399, 468
ApaI	GGGCCC	1 cut(s)	472
ApoI	RAATTY	2 cut(s)	313, 808
Asp700I	GAANNNNTTC	1 cut(s)	764
AspLEI	GCGC	3 cut(s)	190, 331, 491
AspS9I	GGNCC	4 cut(s)	164, 399, 468, 469
AsuC2I	CCSGG	1 cut(s)	776
AsuHPI	GGTGA	4 cut(s)	20, 250, 643, 721
BaeGI	GKGCMC	2 cut(s)	472, 652
BanI	GGYRCC	2 cut(s)	328, 649
BanII	GRGCYC	1 cut(s)	472
BccI	CCATC	7 cut(s)	224, 365, 398, 586, 692, 821, 860
BcgI	CGANNNNNNTGC	4 cut(s)	69, 103, 672, 706
BciT130I	CCWGG	4 cut(s)	32, 397, 486, 646
BclI	TGATCA	1 cut(s)	240
BcnI	CCSGG	1 cut(s)	776
BfaI	CTAG	3 cut(s)	77, 140, 494
BfoI	RGCGCY	2 cut(s)	332, 492
BisI	GCNGC	1 cut(s)	163
BlsI	GCNGC	1 cut(s)	164
Bme1390I	CCNGG	5 cut(s)	32, 397, 486, 646, 776
BmeRI	GACNNNNNGTC	1 cut(s)	301
BmgT120I	GGNCC	4 cut(s)	164, 399, 468, 469
BmiI	GGNNCC	5 cut(s)	330, 470, 471, 483, 651
BmrFI	CCNGG	5 cut(s)	32, 397, 486, 646, 776
BmsI	GCATC	4 cut(s)	85, 137, 199, 542
BoxI	GACNNNNGTC	1 cut(s)	776
BpmI	CTGGAG	1 cut(s)	802
Bpu10I	CCTNAGC	1 cut(s)	726
BpuMI	CCSGG	1 cut(s)	776
BsaHI	GRCGYC	1 cut(s)	329
BsaJI	CCNNGG	4 cut(s)	54, 396, 645, 774
BsaWI	WCCGGW	2 cut(s)	536, 803
Bsc4I	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 324, 478, 659, 780
Bse3DI	GCAATG	2 cut(s)	483, 746
BseBI	CCWGG	4 cut(s)	32, 397, 486, 646
BseDI	CCNNGG	4 cut(s)	54, 396, 645, 774
BseGI	GGATG	3 cut(s)	190, 533, 703
BseLI	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 324, 478, 659, 780
BseMI	GCAATG	2 cut(s)	483, 746
BseMII	CTCAG	1 cut(s)	471
BseSI	GKGCMC	2 cut(s)	472, 652
BseYI	CCCAGC	1 cut(s)	472
BshFI	GGCC	3 cut(s)	165, 401, 470
BshNI	GGYRCC	2 cut(s)	328, 649
BsiSI	CCGG	3 cut(s)	537, 776, 804
BslFI	GGGAC	2 cut(s)	428, 675
BslI	CCNNNNNNNGG	7 cut(s)	172, 200, 201, 324, 478, 659, 780
BsmFI	GGGAC	2 cut(s)	428, 675
BsnI	GGCC	3 cut(s)	165, 401, 470
Bsp120I	GGGCCC	1 cut(s)	468
Bsp1286I	GDGCHC	2 cut(s)	472, 652
Bsp1407I	TGTACA	1 cut(s)	573
Bsp143I	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
Bsp19I	CCATGG	1 cut(s)	54
BspACI	CCGC	4 cut(s)	162, 360, 606, 818
BspANI	GGCC	3 cut(s)	165, 401, 470
BspCNI	CTCAG	1 cut(s)	470
BspHI	TCATGA	1 cut(s)	334
BspLI	GGNNCC	5 cut(s)	330, 470, 471, 483, 651
BspPI	GGATC	3 cut(s)	202, 508, 509
BspT107I	GGYRCC	2 cut(s)	328, 649
BsrDI	GCAATG	2 cut(s)	483, 746
BsrGI	TGTACA	1 cut(s)	573
BssECI	CCNNGG	4 cut(s)	54, 396, 645, 774
BssMI	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
BssNI	GRCGYC	1 cut(s)	329
BssT1I	CCWWGG	1 cut(s)	54
Bst2UI	CCWGG	4 cut(s)	32, 397, 486, 646
Bst4CI	ACNGT	5 cut(s)	40, 556, 623, 706, 892
BstACI	GRCGYC	1 cut(s)	329
BstAUI	TGTACA	1 cut(s)	573
BstC8I	GCNNGC	5 cut(s)	6, 358, 531, 737, 816
BstDEI	CTNAG	4 cut(s)	352, 423, 457, 726
BstDSI	CCRYGG	1 cut(s)	54
BstEII	GGTNACC	1 cut(s)	26
BstF5I	GGATG	3 cut(s)	190, 533, 703
BstH2I	RGCGCY	2 cut(s)	332, 492
BstHHI	GCGC	3 cut(s)	190, 331, 491
BstKTI	GATC	6 cut(s)	18, 210, 243, 433, 503, 517
BstMBI	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
BstMWI	GCNNNNNNNGC	1 cut(s)	488
BstNI	CCWGG	4 cut(s)	32, 397, 486, 646
BstPAI	GACNNNNGTC	1 cut(s)	776
BstPI	GGTNACC	1 cut(s)	26
BstSCI	CCNGG	5 cut(s)	30, 395, 484, 644, 774
BstSLI	GKGCMC	2 cut(s)	472, 652
BstX2I	RGATCY	1 cut(s)	500
BstXI	CCANNNNNNTGG	1 cut(s)	446
BstYI	RGATCY	1 cut(s)	500
BsuRI	GGCC	3 cut(s)	165, 401, 470
BtgI	CCRYGG	1 cut(s)	54
BtsCI	GGATG	3 cut(s)	190, 533, 703
BtsI	GCAGTG	1 cut(s)	390
BtsIMutI	CAGTG	4 cut(s)	36, 390, 628, 711
Cac8I	GCNNGC	5 cut(s)	6, 358, 531, 737, 816
CciI	TCATGA	1 cut(s)	334
CfoI	GCGC	3 cut(s)	190, 331, 491
Cfr13I	GGNCC	4 cut(s)	164, 399, 468, 469
CseI	GACGC	1 cut(s)	62
Csp6I	GTAC	1 cut(s)	574
CviAII	CATG	5 cut(s)	55, 284, 335, 465, 792
CviQI	GTAC	1 cut(s)	574
DdeI	CTNAG	4 cut(s)	352, 423, 457, 726
DinI	GGCGCC	1 cut(s)	330
DpnI	GATC	6 cut(s)	17, 209, 242, 432, 502, 516
DpnII	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
DriI	GACNNNNNGTC	1 cut(s)	301
Eam1105I	GACNNNNNGTC	1 cut(s)	301
Eco130I	CCWWGG	1 cut(s)	54
Eco24I	GRGCYC	1 cut(s)	472
Eco91I	GGTNACC	1 cut(s)	26
EcoO109I	RGGNCCY	1 cut(s)	469
EcoO65I	GGTNACC	1 cut(s)	26
EcoRII	CCWGG	4 cut(s)	30, 395, 484, 644
EcoT14I	CCWWGG	1 cut(s)	54
EcoT38I	GRGCYC	1 cut(s)	472
EgeI	GGCGCC	1 cut(s)	330
EheI	GGCGCC	1 cut(s)	330
ErhI	CCWWGG	1 cut(s)	54
FaeI	CATG	5 cut(s)	58, 287, 338, 468, 795
FaiI	YATR	9 cut(s)	56, 159, 285, 336, 380, 466, 525, 793, 901
FaqI	GGGAC	2 cut(s)	428, 675
FatI	CATG	5 cut(s)	54, 283, 334, 464, 791
FauI	CCCGC	1 cut(s)	613
FbaI	TGATCA	1 cut(s)	240
Fnu4HI	GCNGC	1 cut(s)	163
FokI	GGATG	3 cut(s)	177, 520, 710
FriOI	GRGCYC	1 cut(s)	472
Fsp4HI	GCNGC	1 cut(s)	163
FspBI	CTAG	3 cut(s)	77, 140, 494
GlaI	GCGC	3 cut(s)	189, 330, 490
GluI	GCNGC	1 cut(s)	163
GsaI	CCCAGC	1 cut(s)	476
GsuI	CTGGAG	1 cut(s)	802
HaeII	RGCGCY	2 cut(s)	332, 492
HaeIII	GGCC	3 cut(s)	165, 401, 470
HapII	CCGG	3 cut(s)	537, 776, 804
HgaI	GACGC	1 cut(s)	62
HhaI	GCGC	3 cut(s)	190, 331, 491
Hin1I	GRCGYC	1 cut(s)	329
Hin1II	CATG	5 cut(s)	58, 287, 338, 468, 795
Hin6I	GCGC	3 cut(s)	188, 329, 489
HinP1I	GCGC	3 cut(s)	188, 329, 489
HinfI	GANTC	1 cut(s)	857
HpaII	CCGG	3 cut(s)	537, 776, 804
HphI	GGTGA	4 cut(s)	20, 250, 643, 721
Hpy166II	GTNNAC	2 cut(s)	43, 675
Hpy188I	TCNGA	3 cut(s)	94, 264, 370
Hpy188III	TCNNGA	4 cut(s)	247, 335, 769, 781
Hpy8I	GTNNAC	2 cut(s)	43, 675
HpyAV	CCTTC	7 cut(s)	244, 553, 620, 725, 740, 745, 754
HpyCH4III	ACNGT	5 cut(s)	40, 556, 623, 706, 892
HpyCH4V	TGCA	2 cut(s)	8, 619
HpyF10VI	GCNNNNNNNGC	1 cut(s)	488
HpyF3I	CTNAG	4 cut(s)	352, 423, 457, 726
Hsp92I	GRCGYC	1 cut(s)	329
Hsp92II	CATG	5 cut(s)	58, 287, 338, 468, 795
HspAI	GCGC	3 cut(s)	188, 329, 489
KasI	GGCGCC	1 cut(s)	328
Ksp22I	TGATCA	1 cut(s)	240
Kzo9I	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
LmnI	GCTCC	1 cut(s)	487
LweI	GCATC	4 cut(s)	85, 137, 199, 542
MaeI	CTAG	3 cut(s)	77, 140, 494
MaeIII	GTNAC	4 cut(s)	26, 451, 795, 841
MalI	GATC	6 cut(s)	17, 209, 242, 432, 502, 516
MboI	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
MboII	GAAGA	3 cut(s)	25, 357, 601
MflI	RGATCY	1 cut(s)	500
MhlI	GDGCHC	2 cut(s)	472, 652
MluCI	AATT	3 cut(s)	111, 313, 808
Mly113I	GGCGCC	1 cut(s)	329
MmeI	TCCRAC	2 cut(s)	100, 427
MnlI	CCTC	5 cut(s)	169, 247, 342, 466, 777
MroXI	GAANNNNTTC	1 cut(s)	764
MseI	TTAA	2 cut(s)	105, 291
MspI	CCGG	3 cut(s)	537, 776, 804
MspR9I	CCNGG	5 cut(s)	32, 397, 486, 646, 776
MvaI	CCWGG	4 cut(s)	32, 397, 486, 646
MwoI	GCNNNNNNNGC	1 cut(s)	488
NarI	GGCGCC	1 cut(s)	329
NciI	CCSGG	1 cut(s)	776
NcoI	CCATGG	1 cut(s)	54
NdeII	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
NlaIII	CATG	5 cut(s)	58, 287, 338, 468, 795
NlaIV	GGNNCC	5 cut(s)	330, 470, 471, 483, 651
NmuCI	GTSAC	2 cut(s)	26, 795
PagI	TCATGA	1 cut(s)	334
PdmI	GAANNNNTTC	1 cut(s)	764
PfeI	GAWTC	1 cut(s)	857
PflFI	GACNNNGTC	1 cut(s)	38
PflMI	CCANNNNNTGG	1 cut(s)	324
PkrI	GCNGC	1 cut(s)	164
PluTI	GGCGCC	1 cut(s)	332
PshAI	GACNNNNGTC	1 cut(s)	776
Psp6I	CCWGG	4 cut(s)	30, 395, 484, 644
PspEI	GGTNACC	1 cut(s)	26
PspFI	CCCAGC	1 cut(s)	472
PspGI	CCWGG	4 cut(s)	30, 395, 484, 644
PspN4I	GGNNCC	5 cut(s)	330, 470, 471, 483, 651
PspOMI	GGGCCC	1 cut(s)	468
PspPI	GGNCC	4 cut(s)	164, 399, 468, 469
PsuI	RGATCY	1 cut(s)	500
PsyI	GACNNNGTC	1 cut(s)	38
RsaI	GTAC	1 cut(s)	575
RsaNI	GTAC	1 cut(s)	574
SaqAI	TTAA	2 cut(s)	105, 291
SatI	GCNGC	1 cut(s)	163
Sau3AI	GATC	6 cut(s)	15, 207, 240, 430, 500, 514
Sau96I	GGNCC	4 cut(s)	164, 399, 468, 469
ScrFI	CCNGG	5 cut(s)	32, 397, 486, 646, 776
SduI	GDGCHC	2 cut(s)	472, 652
SfaNI	GCATC	4 cut(s)	85, 137, 199, 542
SfoI	GGCGCC	1 cut(s)	330
Sse9I	AATT	3 cut(s)	111, 313, 808
SsiI	CCGC	4 cut(s)	162, 360, 606, 818
SspDI	GGCGCC	1 cut(s)	328
SspMI	CTAG	3 cut(s)	77, 140, 494
StyD4I	CCNGG	5 cut(s)	30, 395, 484, 644, 774
StyI	CCWWGG	1 cut(s)	54
TaaI	ACNGT	5 cut(s)	40, 556, 623, 706, 892
TaqI	TCGA	4 cut(s)	18, 206, 864, 894
TasI	AATT	3 cut(s)	111, 313, 808
TatI	WGTACW	1 cut(s)	573
TauI	GCSGC	1 cut(s)	165
TfiI	GAWTC	1 cut(s)	857
Tru1I	TTAA	2 cut(s)	105, 291
Tru9I	TTAA	2 cut(s)	105, 291
TscAI	CASTG	4 cut(s)	43, 397, 628, 711
TseFI	GTSAC	2 cut(s)	26, 795
Tsp45I	GTSAC	2 cut(s)	26, 795
TspDTI	ATGAA	3 cut(s)	351, 453, 512
TspGWI	ACGGA	1 cut(s)	81
TspRI	CASTG	4 cut(s)	43, 397, 628, 711
Tth111I	GACNNNGTC	1 cut(s)	38
Van91I	CCANNNNNTGG	1 cut(s)	324
XapI	RAATTY	2 cut(s)	313, 808
XmnI	GAANNNNTTC	1 cut(s)	764
XspI	CTAG	3 cut(s)	77, 140, 494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.