Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001175

Physical Location & Seq

Genomic Coordinates Reverse (-)

13935156 .. 13938845

Gene Structure 3690 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G32850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGTGGAAGTTTAAACCTTTTGCTCAAAAAGAACCTGCGGGTCTCGAAGGTCGATTTCTCGAGATAGGAAATCTGAAAGTTCAAGTTAGAAATGTCATTGCGGAAGGAGGTTTCTCCAGTGTTTACTTAGCTCAAGATGTAAATCACGCGTCAAAGCAATATGCTTTGAAGCACATGATATGCAATGACGAGGAGTCACTTGAACTCGTAATGAAAGAGATCTCGGTTCTGAAATCCCTCAAGGGACATCCAAATGTTGTCACGCTCTACGCACATGGTATCTTGGATATGGGGAGGAATAAAAAAGAAGCTCTTTTGGCTATGGATTTTTGTGGTAAATCTCTTGTGGATGTGCTTGAGAATAGAGGTGCTGGTTACTTTGAAGAGAAACAAGCTCTTACGATTTTTAGAGATGTGTGTAATGCTGTCTTTGCAATGCATTGTCAATCCCCACGCATTGCTCACAGAGACTTGAAGGCTGAGAATCTTCTATTGAGCTCAGATGGACAATGGAAATTGTGTGATTTTGGAAGCGTTTCGACAAATCACAAGATTTTTGAAAGAGCAGAAGAGATGGGAATTGAAGAAGATAATATTAGAAAATACACAACACCAACATATAGAGCTCCTGAGATGTGGGATCTCTTTCGAAGGGAGATGATAAGCGAGAAGGTCGATATATGGGCTCTTGGATGTCTCTTGTTTCGGATATGCTATTTCAAGAATGCATTTGATGGAGAATCAAAGCTGCAAATCTTAAACGGGAATTATCGTATTCCAGAATCGCCTAAATACAGTGTATTTATTACGGATCTCATCAAAGAAATGCTCCAAGCTTCACCCGATGAACGACCAGATATTACACAGATTTGGTTTCGTGTCAATGAGCAGCTTCCTGCTAATTTACAGAAATCATTACCGGATCGACCACCTGAAATGCAATCTACTGGAGTCCATGATGGCTCATCAAAATCAGCAACTAAACCTTCTCCAGCACCTCGTAGAAGTCCGCCACCGCCGCCACCATCTTCTGGAGAATCAGATAGTGGAGGACCGCTCGGTGCCTTTTGGGCGACTCAGCATGCTAAAACCTCAGTTGTATCAGAAGATAACAAAAACATGCCTAAATTTGATGAACCAAACAGTAACACATCTAAATCTGAAAGAGTTCGTGTGGATAGTCATCAACCCAAAAAACCAAGTCCTGTGAGAGGAGAGGCTCGTGGTATTCAAAGAAATAAAGATCTTGAAACTACGATTTCTCAGAAGAATACAACTCCTGCTGCAGCCAATAACATGACAAGAGTGTCCAAGGATGATGCCTTCAACTCGTTTGTTGCTGACTTTGATACCACAAAGTTCGATAATGGTAATAAACCTGGCAAAGAAGAGGCATTGGAAGCTGAGATACAGAGGTTAAAAGACGAGTTGAAGCAAACAAAATCAGAGAAGGCAGAAATAACTGCTAAGTTTGAAAAGCTTTCTGCTATCTGCAGATCGCAAAGGCAAGAGTTGCAGGATCTCAAACAAACACTTGCCTCTAAAAGTGCCTCACCGTCACCGAGCAGAGACTCTTCACAAAATCAACCCTCTCCAGGGATGCATTCCATGTCATCAACTCCATCGAGAGATAAGATGGAAGGAACAATGTGGGAACTTCAACAGGACAGATCTAACTGGTCAACTGGCAGCTCTGATACAAATTCATGGCAACCTTTTAGTGATGAAGCGAAACCCGTGATGGAATCTGCATCAAAGGGTACTCAAGGTTTTGAGCCATGGGGTTTCGAGACAGAATCCTTTAGAGCCGCTGCAACGTCTGCAGCTGCTACTTCTGCATCTGGAACACAAAGATCTATGGGCTCTGGAAACAGCACTTCACAGAGATATGGGAACTCAAAGATGAGAGAAAACCAGAAAACAGCTCAACCTGCTGGATGGGCCGGCTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030100 GO:0032879 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0060627 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

650

Amino Acids

72.44

Weight (kDa)

7.17

Isoelectric Point (pI)

48.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	28 - 290	1.6e-25	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	30 - 290	1.8e-42	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32850 AT2G32850
fragaria_vesca	FvH4_7g00200 FvH4_7g00210 FvH4_7g00210
malus_domestica	MD02G1318100.v1.1 MD14G1085700.v1.1 MD14G1085800.v1.1
prunus_persica	Prupe.2G004700_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1
pyrus_communis	pycom02g26640 pycom14g07560
rosa_chinensis	RchiOBHm_Chr1g0313461 RchiOBHm_Chr1g0313481 RchiOBHm_Chr1g0313501 RchiOBHm_Chr1g0313511 RchiOBHm_Chr1g0313531 RchiOBHm_Chr1g0313561 RchiOBHm_Chr1g0313571 RchiOBHm_Chr1g0313591 RchiOBHm_Chr1g0313601 RchiOBHm_Chr1g0313611 RchiOBHm_Chr1g0313621 RchiOBHm_Chr1g0313631 RchiOBHm_Chr1g0372901
rosa_laevigata	RLG00000026867 RLG00000030789 RLG00000030790 RLG00000030791
rosa_multiflora	Rmu_co8009002.1_g000001 Rmu_sc0000931.1_g000014 Rmu_sc0000931.1_g000018 Rmu_sc0015122.1_g000001
rosa_roxburghii	Rroxscaffold_4G00284640 Rroxscaffold_4G00332160
rosa_rugosa	Rorug01G0374800 RorugPtG0005200.1 RorugPtG0005300.1 RorugPtG0005400.1
rosa_samantha	Rh1AG003900 Rh1AG005500 Rh1BG000900 Rh1CG000500 Rh1CG000700 Rh1CG008400 Rh1DG003600 Rh1DG003700 Rh1DG003800 Rh1DG378100
rosa_wichuraiana	Rw1G000310 Rw1G000320 Rw1G000330 Rw1G033770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1306
Acc36I	ACCTGC	2 cut(s)	43, 1939
AccB1I	GGYRCC	1 cut(s)	1061
AccB7I	CCANNNNNTGG	1 cut(s)	1031
AccBSI	CCGCTC	1 cut(s)	1057
AccII	CGCG	1 cut(s)	149
AciI	CCGC	7 cut(s)	38, 101, 1010, 1016, 1019, 1055, 1809
AclWI	GGATC	4 cut(s)	648, 819, 930, 1527
AcsI	RAATTY	2 cut(s)	1127, 1702
AfaI	GTAC	1 cut(s)	1762
AfiI	CCNNNNNNNGG	4 cut(s)	1031, 1211, 1595, 1596
AflIII	ACRYGT	1 cut(s)	147
AhdI	GACNNNNNGTC	1 cut(s)	193
AjnI	CCWGG	2 cut(s)	1378, 1594
Alw21I	GWGCWC	2 cut(s)	500, 628
Alw26I	GTCTC	5 cut(s)	47, 462, 701, 1563, 1784
AlwI	GGATC	4 cut(s)	648, 819, 930, 1527
Ama87I	CYCGRG	1 cut(s)	59
AoxI	GGCC	1 cut(s)	1941
ApeKI	GCWGC	8 cut(s)	748, 889, 1283, 1286, 1689, 1811, 1823, 1826
ApoI	RAATTY	2 cut(s)	1127, 1702
Asp700I	GAANNNNTTC	2 cut(s)	535, 1167
AspS9I	GGNCC	2 cut(s)	1052, 1941
AsuHPI	GGTGA	3 cut(s)	831, 1545, 1551
AsuII	TTCGAA	1 cut(s)	649
AvaI	CYCGRG	1 cut(s)	59
AvaII	GGWCC	1 cut(s)	1052
BaeI	ACNNNNGTAYC	2 cut(s)	1400, 1433
BanI	GGYRCC	1 cut(s)	1061
BanII	GRGCYC	4 cut(s)	500, 628, 688, 1865
BarI	GAAGNNNNNNTAC	2 cut(s)	1392, 1424
BauI	CACGAG	1 cut(s)	1221
Bbv12I	GWGCWC	2 cut(s)	500, 628
BbvI	GCAGC	8 cut(s)	735, 901, 1270, 1298, 1701, 1798, 1813, 1835
BccI	CCATC	9 cut(s)	497, 568, 728, 953, 1033, 1630, 1630, 1735, 1932
BciT130I	CCWGG	2 cut(s)	1380, 1596
BcoDI	GTCTC	5 cut(s)	47, 462, 701, 1563, 1784
BfmI	CTRYAG	3 cut(s)	1284, 1492, 1821
BfuAI	ACCTGC	2 cut(s)	43, 1939
BglI	GCCNNNNNGGC	1 cut(s)	1070
BglII	AGATCT	4 cut(s)	219, 1243, 1670, 1853
Bme1390I	CCNGG	2 cut(s)	1380, 1596
Bme18I	GGWCC	1 cut(s)	1052
BmeRI	GACNNNNNGTC	1 cut(s)	193
BmeT110I	CYCGRG	1 cut(s)	59
BmgT120I	GGNCC	2 cut(s)	1052, 1941
BmiI	GGNNCC	1 cut(s)	1063
BmrFI	CCNGG	2 cut(s)	1380, 1596
BmsI	GCATC	4 cut(s)	1309, 1590, 1760, 1847
BplI	GAGNNNNNCTC	2 cut(s)	1041, 1073
BpmI	CTGGAG	5 cut(s)	100, 969, 975, 1053, 1578
Bpu14I	TTCGAA	1 cut(s)	649
BpuEI	CTTGAG	4 cut(s)	117, 224, 377, 1749
BsaBI	GATNNNNATC	2 cut(s)	141, 1182
BsaI	GGTCTC	1 cut(s)	47
BsaJI	CCNNGG	3 cut(s)	1311, 1595, 1778
BsaWI	WCCGGW	1 cut(s)	919
Bsc4I	CCNNNNNNNGG	4 cut(s)	1031, 1211, 1595, 1596
Bse118I	RCCGGY	1 cut(s)	1943
Bse1I	ACTGG	4 cut(s)	117, 952, 1682, 1690
Bse3DI	GCAATG	4 cut(s)	96, 190, 441, 456
Bse8I	GATNNNNATC	2 cut(s)	141, 1182
BseBI	CCWGG	2 cut(s)	1380, 1596
BseDI	CCNNGG	3 cut(s)	1311, 1595, 1778
BseGI	GGATG	6 cut(s)	247, 355, 698, 1321, 1605, 1943
BseJI	GATNNNNATC	2 cut(s)	141, 1182
BseLI	CCNNNNNNNGG	4 cut(s)	1031, 1211, 1595, 1596
BseMI	GCAATG	4 cut(s)	96, 190, 441, 456
BseMII	CTCAG	7 cut(s)	471, 513, 621, 1091, 1107, 1277, 1395
BseNI	ACTGG	4 cut(s)	117, 952, 1682, 1690
BseRI	GAGGAG	2 cut(s)	206, 1227
BseXI	GCAGC	8 cut(s)	735, 901, 1270, 1298, 1701, 1798, 1813, 1835
Bsh1236I	CGCG	1 cut(s)	149
BshFI	GGCC	1 cut(s)	1943
BshNI	GGYRCC	1 cut(s)	1061
BsiHKAI	GWGCWC	2 cut(s)	500, 628
BsiHKCI	CYCGRG	1 cut(s)	59
BsiSI	CCGG	2 cut(s)	920, 1944
BslFI	GGGAC	1 cut(s)	258
BslI	CCNNNNNNNGG	4 cut(s)	1031, 1211, 1595, 1596
BsmAI	GTCTC	5 cut(s)	47, 462, 701, 1563, 1784
BsmFI	GGGAC	1 cut(s)	258
BsmI	GAATGC	2 cut(s)	730, 1603
BsnI	GGCC	1 cut(s)	1943
Bso31I	GGTCTC	1 cut(s)	47
BsoBI	CYCGRG	1 cut(s)	59
Bsp119I	TTCGAA	1 cut(s)	649
Bsp1286I	GDGCHC	4 cut(s)	500, 628, 688, 1865
Bsp143I	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
Bsp19I	CCATGG	1 cut(s)	1778
BspACI	CCGC	7 cut(s)	38, 101, 1010, 1016, 1019, 1055, 1809
BspANI	GGCC	1 cut(s)	1943
BspCNI	CTCAG	7 cut(s)	472, 512, 622, 1090, 1106, 1276, 1396
BspFNI	CGCG	1 cut(s)	149
BspLI	GGNNCC	1 cut(s)	1063
BspMAI	CTGCAG	3 cut(s)	1288, 1496, 1825
BspMI	ACCTGC	2 cut(s)	43, 1939
BspPI	GGATC	4 cut(s)	648, 819, 930, 1527
BspT104I	TTCGAA	1 cut(s)	649
BspT107I	GGYRCC	1 cut(s)	1061
BspTNI	GGTCTC	1 cut(s)	47
BsrBI	CCGCTC	1 cut(s)	1057
BsrDI	GCAATG	4 cut(s)	96, 190, 441, 456
BsrFI	RCCGGY	1 cut(s)	1943
BsrI	ACTGG	4 cut(s)	117, 952, 1682, 1690
BssAI	RCCGGY	1 cut(s)	1943
BssECI	CCNNGG	3 cut(s)	1311, 1595, 1778
BssMI	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
BssSI	CACGAG	1 cut(s)	1221
BssT1I	CCWWGG	2 cut(s)	1311, 1778
Bst2BI	CACGAG	1 cut(s)	1221
Bst2UI	CCWGG	2 cut(s)	1380, 1596
Bst4CI	ACNGT	3 cut(s)	797, 1145, 1557
Bst6I	CTCTTC	4 cut(s)	378, 564, 1383, 1579
BstAPI	GCANNNNNTGC	2 cut(s)	1513, 1820
BstBI	TTCGAA	1 cut(s)	649
BstC8I	GCNNGC	2 cut(s)	1083, 1945
BstDEI	CTNAG	9 cut(s)	127, 480, 499, 630, 1077, 1093, 1263, 1404, 1467
BstDSI	CCRYGG	1 cut(s)	1778
BstENI	CCTNNNNNAGG	1 cut(s)	1209
BstF5I	GGATG	6 cut(s)	247, 355, 698, 1321, 1605, 1943
BstFNI	CGCG	1 cut(s)	149
BstKTI	GATC	9 cut(s)	222, 643, 814, 925, 1246, 1499, 1522, 1673, 1856
BstMAI	GTCTC	5 cut(s)	47, 462, 701, 1563, 1784
BstMBI	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
BstNI	CCWGG	2 cut(s)	1380, 1596
BstNSI	RCATGY	2 cut(s)	1085, 1123
BstSCI	CCNGG	2 cut(s)	1378, 1594
BstSFI	CTRYAG	3 cut(s)	1284, 1492, 1821
BstUI	CGCG	1 cut(s)	149
BstV1I	GCAGC	8 cut(s)	735, 901, 1270, 1298, 1701, 1798, 1813, 1835
BstX2I	RGATCY	7 cut(s)	219, 640, 811, 1243, 1519, 1670, 1853
BstYI	RGATCY	7 cut(s)	219, 640, 811, 1243, 1519, 1670, 1853
BsuRI	GGCC	1 cut(s)	1943
BtgI	CCRYGG	1 cut(s)	1778
BtsCI	GGATG	6 cut(s)	247, 355, 698, 1321, 1605, 1943
BtsIMutI	CAGTG	2 cut(s)	124, 802
BveI	ACCTGC	2 cut(s)	43, 1939
Cac8I	GCNNGC	2 cut(s)	1083, 1945
Cfr10I	RCCGGY	1 cut(s)	1943
Cfr13I	GGNCC	2 cut(s)	1052, 1941
CseI	GACGC	1 cut(s)	138
Csp6I	GTAC	1 cut(s)	1761
CviAII	CATG	9 cut(s)	175, 275, 956, 1082, 1120, 1297, 1609, 1707, 1779
CviQI	GTAC	1 cut(s)	1761
DdeI	CTNAG	9 cut(s)	127, 480, 499, 630, 1077, 1093, 1263, 1404, 1467
DpnI	GATC	9 cut(s)	221, 642, 813, 924, 1245, 1498, 1521, 1672, 1855
DpnII	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
DraI	TTTAAA	1 cut(s)	13
DrdI	GACNNNNNNGTC	1 cut(s)	1306
DriI	GACNNNNNGTC	1 cut(s)	193
DseDI	GACNNNNNNGTC	1 cut(s)	1306
Eam1104I	CTCTTC	4 cut(s)	378, 564, 1383, 1579
Eam1105I	GACNNNNNGTC	1 cut(s)	193
EarI	CTCTTC	4 cut(s)	378, 564, 1383, 1579
EciI	GGCGGA	1 cut(s)	999
Ecl136II	GAGCTC	2 cut(s)	498, 626
Eco130I	CCWWGG	2 cut(s)	1311, 1778
Eco24I	GRGCYC	4 cut(s)	500, 628, 688, 1865
Eco31I	GGTCTC	1 cut(s)	47
Eco47I	GGWCC	1 cut(s)	1052
Eco53kI	GAGCTC	2 cut(s)	498, 626
Eco88I	CYCGRG	1 cut(s)	59
EcoICRI	GAGCTC	2 cut(s)	498, 626
EcoNI	CCTNNNNNAGG	1 cut(s)	1209
EcoRII	CCWGG	2 cut(s)	1378, 1594
EcoT14I	CCWWGG	2 cut(s)	1311, 1778
EcoT22I	ATGCAT	3 cut(s)	441, 730, 1605
EcoT38I	GRGCYC	4 cut(s)	500, 628, 688, 1865
ErhI	CCWWGG	2 cut(s)	1311, 1778
FaeI	CATG	9 cut(s)	178, 278, 959, 1085, 1123, 1300, 1612, 1710, 1782
FalI	AAGNNNNNCTT	2 cut(s)	297, 329
FaqI	GGGAC	1 cut(s)	258
FatI	CATG	9 cut(s)	174, 274, 955, 1081, 1119, 1296, 1608, 1706, 1778
FauI	CCCGC	1 cut(s)	31
FokI	GGATG	5 cut(s)	234, 362, 705, 1328, 1612
FriOI	GRGCYC	4 cut(s)	500, 628, 688, 1865
GsuI	CTGGAG	5 cut(s)	100, 969, 975, 1053, 1578
HaeIII	GGCC	1 cut(s)	1943
HapII	CCGG	2 cut(s)	920, 1944
HgaI	GACGC	1 cut(s)	138
Hin1II	CATG	9 cut(s)	178, 278, 959, 1085, 1123, 1300, 1612, 1710, 1782
HincII	GTYRAC	1 cut(s)	1683
HindII	GTYRAC	1 cut(s)	1683
HindIII	AAGCTT	2 cut(s)	834, 1478
HpaII	CCGG	2 cut(s)	920, 1944
HphI	GGTGA	3 cut(s)	831, 1545, 1551
Hpy166II	GTNNAC	2 cut(s)	124, 1683
Hpy8I	GTNNAC	2 cut(s)	124, 1683
HpyAV	CCTTC	9 cut(s)	41, 98, 469, 645, 664, 996, 1333, 1444, 1634
HpyCH4III	ACNGT	3 cut(s)	797, 1145, 1557
HpyCH4IV	ACGT	1 cut(s)	1817
HpyF3I	CTNAG	9 cut(s)	127, 480, 499, 630, 1077, 1093, 1263, 1404, 1467
HpySE526I	ACGT	1 cut(s)	1817
Hsp92II	CATG	9 cut(s)	178, 278, 959, 1085, 1123, 1300, 1612, 1710, 1782
KroI	GCCGGC	1 cut(s)	1943
KroNI	GCCGGC	1 cut(s)	1945
Kzo9I	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
LmnI	GCTCC	2 cut(s)	631, 834
Lsp1109I	GCAGC	8 cut(s)	735, 901, 1270, 1298, 1701, 1798, 1813, 1835
LweI	GCATC	4 cut(s)	1309, 1590, 1760, 1847
MaeII	ACGT	1 cut(s)	1817
MaeIII	GTNAC	5 cut(s)	195, 259, 374, 1145, 1557
MalI	GATC	9 cut(s)	221, 642, 813, 924, 1245, 1498, 1521, 1672, 1855
MbiI	CCGCTC	1 cut(s)	1057
MboI	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
MflI	RGATCY	7 cut(s)	219, 640, 811, 1243, 1519, 1670, 1853
MhlI	GDGCHC	4 cut(s)	500, 628, 688, 1865
MluCI	AATT	6 cut(s)	515, 579, 766, 901, 1127, 1702
MluI	ACGCGT	1 cut(s)	147
MlyI	GAGTC	4 cut(s)	203, 960, 1069, 1565
Mph1103I	ATGCAT	3 cut(s)	441, 730, 1605
MroNI	GCCGGC	1 cut(s)	1943
MroXI	GAANNNNTTC	2 cut(s)	535, 1167
MseI	TTAA	3 cut(s)	12, 758, 1418
MslI	CAYNNNNRTG	1 cut(s)	252
MspA1I	CMGCKG	2 cut(s)	1811, 1826
MspI	CCGG	2 cut(s)	920, 1944
MspR9I	CCNGG	2 cut(s)	1380, 1596
MssI	GTTTAAAC	1 cut(s)	13
Mva1269I	GAATGC	2 cut(s)	730, 1603
MvaI	CCWGG	2 cut(s)	1380, 1596
MvnI	CGCG	1 cut(s)	149
NaeI	GCCGGC	1 cut(s)	1945
NcoI	CCATGG	1 cut(s)	1778
NdeII	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
NgoMIV	GCCGGC	1 cut(s)	1943
NlaIII	CATG	9 cut(s)	178, 278, 959, 1085, 1123, 1300, 1612, 1710, 1782
NlaIV	GGNNCC	1 cut(s)	1063
NmuCI	GTSAC	3 cut(s)	195, 259, 1557
NsiI	ATGCAT	3 cut(s)	441, 730, 1605
NspI	RCATGY	2 cut(s)	1085, 1123
NspV	TTCGAA	1 cut(s)	649
PaeI	GCATGC	1 cut(s)	1085
PaeR7I	CTCGAG	1 cut(s)	59
PctI	GAATGC	2 cut(s)	730, 1603
PdiI	GCCGGC	1 cut(s)	1945
PdmI	GAANNNNTTC	2 cut(s)	535, 1167
PfeI	GAWTC	6 cut(s)	484, 740, 782, 1037, 1745, 1796
PflMI	CCANNNNNTGG	1 cut(s)	1031
PleI	GAGTC	4 cut(s)	202, 959, 1069, 1565
PmeI	GTTTAAAC	1 cut(s)	13
PpsI	GAGTC	4 cut(s)	202, 959, 1069, 1565
Psp124BI	GAGCTC	2 cut(s)	500, 628
Psp6I	CCWGG	2 cut(s)	1378, 1594
PspGI	CCWGG	2 cut(s)	1378, 1594
PspN4I	GGNNCC	1 cut(s)	1063
PspPI	GGNCC	2 cut(s)	1052, 1941
PstI	CTGCAG	3 cut(s)	1288, 1496, 1825
PsuI	RGATCY	7 cut(s)	219, 640, 811, 1243, 1519, 1670, 1853
PvuII	CAGCTG	1 cut(s)	1826
RsaI	GTAC	1 cut(s)	1762
RsaNI	GTAC	1 cut(s)	1761
RseI	CAYNNNNRTG	1 cut(s)	252
SacI	GAGCTC	2 cut(s)	500, 628
SaqAI	TTAA	3 cut(s)	12, 758, 1418
Sau3AI	GATC	9 cut(s)	219, 640, 811, 922, 1243, 1496, 1519, 1670, 1853
Sau96I	GGNCC	2 cut(s)	1052, 1941
SchI	GAGTC	4 cut(s)	203, 960, 1069, 1565
ScrFI	CCNGG	2 cut(s)	1380, 1596
SduI	GDGCHC	4 cut(s)	500, 628, 688, 1865
SfaNI	GCATC	4 cut(s)	1309, 1590, 1760, 1847
SfcI	CTRYAG	3 cut(s)	1284, 1492, 1821
Sfr274I	CTCGAG	1 cut(s)	59
SfuI	TTCGAA	1 cut(s)	649
SinI	GGWCC	1 cut(s)	1052
SlaI	CTCGAG	1 cut(s)	59
SmiMI	CAYNNNNRTG	1 cut(s)	252
SmlI	CTYRAG	5 cut(s)	59, 132, 239, 356, 1764
SmoI	CTYRAG	5 cut(s)	59, 132, 239, 356, 1764
SphI	GCATGC	1 cut(s)	1085
Sse9I	AATT	6 cut(s)	515, 579, 766, 901, 1127, 1702
SsiI	CCGC	7 cut(s)	38, 101, 1010, 1016, 1019, 1055, 1809
SspI	AATATT	1 cut(s)	595
SstI	GAGCTC	2 cut(s)	500, 628
StyD4I	CCNGG	2 cut(s)	1378, 1594
StyI	CCWWGG	2 cut(s)	1311, 1778
TaaI	ACNGT	3 cut(s)	797, 1145, 1557
TaiI	ACGT	1 cut(s)	1820
TasI	AATT	6 cut(s)	515, 579, 766, 901, 1127, 1702
TauI	GCSGC	2 cut(s)	1021, 1811
TfiI	GAWTC	6 cut(s)	484, 740, 782, 1037, 1745, 1796
Tru1I	TTAA	3 cut(s)	12, 758, 1418
Tru9I	TTAA	3 cut(s)	12, 758, 1418
TscAI	CASTG	2 cut(s)	124, 802
TseFI	GTSAC	3 cut(s)	195, 259, 1557
TseI	GCWGC	8 cut(s)	748, 889, 1283, 1286, 1689, 1811, 1823, 1826
Tsp45I	GTSAC	3 cut(s)	195, 259, 1557
TspDTI	ATGAA	5 cut(s)	227, 861, 1149, 1695, 1740
TspGWI	ACGGA	1 cut(s)	824
TspRI	CASTG	2 cut(s)	124, 802
Van91I	CCANNNNNTGG	1 cut(s)	1031
VpaK11BI	GGWCC	1 cut(s)	1052
XagI	CCTNNNNNAGG	1 cut(s)	1209
XapI	RAATTY	2 cut(s)	1127, 1702
XceI	RCATGY	2 cut(s)	1085, 1123
XhoI	CTCGAG	1 cut(s)	59
XmnI	GAANNNNTTC	2 cut(s)	535, 1167
Zsp2I	ATGCAT	3 cut(s)	441, 730, 1605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.