Protein kinase domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 3 cut(s) | 793, 1027, 1843 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1991, 2926 |
| AatII | GACGTC | 1 cut(s) | 240 |
| Acc16I | TGCGCA | 1 cut(s) | 2223 |
| Acc36I | ACCTGC | 6 cut(s) | 793, 1027, 1843, 2209, 2636, 3302 |
| AccBSI | CCGCTC | 2 cut(s) | 228, 2916 |
| AccI | GTMKAC | 1 cut(s) | 846 |
| AccII | CGCG | 3 cut(s) | 2099, 3093, 3588 |
| AclWI | GGATC | 6 cut(s) | 819, 2418, 2431, 3068, 4313, 4326 |
| AcsI | RAATTY | 6 cut(s) | 12, 1452, 1474, 1580, 1715, 3752 |
| AcuI | CTGAAG | 5 cut(s) | 562, 759, 986, 1179, 1671 |
| AcvI | CACGTG | 1 cut(s) | 1426 |
| AcyI | GRCGYC | 1 cut(s) | 237 |
| AdeI | CACNNNGTG | 1 cut(s) | 1271 |
| AfaI | GTAC | 6 cut(s) | 5, 525, 592, 2650, 3959, 4386 |
| AfeI | AGCGCT | 1 cut(s) | 725 |
| AflII | CTTAAG | 1 cut(s) | 1769 |
| AflIII | ACRYGT | 1 cut(s) | 1423 |
| AhlI | ACTAGT | 1 cut(s) | 1541 |
| AjnI | CCWGG | 2 cut(s) | 2924, 3055 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 647, 679, 2420, 2452 |
| AleI | CACNNNNGTG | 2 cut(s) | 1037, 3120 |
| Alw21I | GWGCWC | 4 cut(s) | 95, 3457, 3609, 3695 |
| Alw26I | GTCTC | 7 cut(s) | 516, 987, 1640, 1931, 2256, 2397, 4324 |
| AlwI | GGATC | 6 cut(s) | 819, 2418, 2431, 3068, 4313, 4326 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3012 |
| Ama87I | CYCGRG | 2 cut(s) | 306, 3402 |
| Aor51HI | AGCGCT | 1 cut(s) | 725 |
| ApaI | GGGCCC | 1 cut(s) | 799 |
| ApoI | RAATTY | 6 cut(s) | 12, 1452, 1474, 1580, 1715, 3752 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3786, 3818 |
| AseI | ATTAAT | 1 cut(s) | 2505 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1795, 2146, 3051 |
| AspLEI | GCGC | 5 cut(s) | 726, 2101, 2224, 3093, 3590 |
| AsuC2I | CCSGG | 1 cut(s) | 2296 |
| AsuNHI | GCTAGC | 1 cut(s) | 3878 |
| AvaI | CYCGRG | 2 cut(s) | 306, 3402 |
| AvaII | GGWCC | 5 cut(s) | 310, 565, 1281, 2928, 3291 |
| BaeGI | GKGCMC | 2 cut(s) | 799, 1375 |
| BamHI | GGATCC | 2 cut(s) | 2423, 4318 |
| BanII | GRGCYC | 5 cut(s) | 799, 859, 3609, 3695, 3954 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 694, 726, 3567, 3599, 4167, 4199 |
| BauI | CACGAG | 1 cut(s) | 2065 |
| BbrPI | CACGTG | 1 cut(s) | 1426 |
| BbsI | GAAGAC | 2 cut(s) | 765, 2752 |
| Bbv12I | GWGCWC | 4 cut(s) | 95, 3457, 3609, 3695 |
| BbvCI | CCTCAGC | 1 cut(s) | 1296 |
| BceAI | ACGGC | 5 cut(s) | 87, 123, 982, 1823, 1939 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1137, 1171, 4029, 4063 |
| BciT130I | CCWGG | 2 cut(s) | 2926, 3057 |
| BclI | TGATCA | 2 cut(s) | 2329, 3427 |
| BcnI | CCSGG | 1 cut(s) | 2296 |
| BcoDI | GTCTC | 7 cut(s) | 516, 987, 1640, 1931, 2256, 2397, 4324 |
| BcuI | ACTAGT | 1 cut(s) | 1541 |
| BfmI | CTRYAG | 6 cut(s) | 99, 594, 790, 920, 2679, 4138 |
| BfoI | RGCGCY | 1 cut(s) | 727 |
| BfrI | CTTAAG | 1 cut(s) | 1769 |
| BfuAI | ACCTGC | 6 cut(s) | 793, 1027, 1843, 2209, 2636, 3302 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 222, 794, 3057 |
| BglII | AGATCT | 2 cut(s) | 133, 924 |
| Bme1390I | CCNGG | 3 cut(s) | 2296, 2926, 3057 |
| Bme18I | GGWCC | 5 cut(s) | 310, 565, 1281, 2928, 3291 |
| BmeT110I | CYCGRG | 2 cut(s) | 306, 3402 |
| BmrFI | CCNGG | 3 cut(s) | 2296, 2926, 3057 |
| BmrI | ACTGGG | 2 cut(s) | 3183, 4204 |
| BmtI | GCTAGC | 1 cut(s) | 3882 |
| BmuI | ACTGGG | 2 cut(s) | 3183, 4204 |
| BpiI | GAAGAC | 2 cut(s) | 765, 2752 |
| BpmI | CTGGAG | 3 cut(s) | 395, 1883, 4298 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1296 |
| BpuEI | CTTGAG | 4 cut(s) | 233, 2028, 2541, 2675 |
| BpuMI | CCSGG | 1 cut(s) | 2296 |
| BsaAI | YACGTR | 1 cut(s) | 1426 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1347 |
| BsaHI | GRCGYC | 1 cut(s) | 237 |
| BsaI | GGTCTC | 1 cut(s) | 4324 |
| BsaJI | CCNNGG | 4 cut(s) | 1242, 1498, 2295, 2471 |
| BsaWI | WCCGGW | 1 cut(s) | 1917 |
| Bse1I | ACTGG | 9 cut(s) | 1067, 2110, 2470, 2977, 3189, 3341, 3564, 3879, 4199 |
| Bse3DI | GCAATG | 3 cut(s) | 270, 612, 627 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1347 |
| BseBI | CCWGG | 2 cut(s) | 2926, 3057 |
| BseDI | CCNNGG | 4 cut(s) | 1242, 1498, 2295, 2471 |
| BseJI | GATNNNNATC | 1 cut(s) | 1347 |
| BseMI | GCAATG | 3 cut(s) | 270, 612, 627 |
| BseNI | ACTGG | 9 cut(s) | 1067, 2110, 2470, 2977, 3189, 3341, 3564, 3879, 4199 |
| BseRI | GAGGAG | 4 cut(s) | 85, 552, 4159, 4265 |
| BseSI | GKGCMC | 2 cut(s) | 799, 1375 |
| BseYI | CCCAGC | 2 cut(s) | 2493, 3287 |
| BsgI | GTGCAG | 3 cut(s) | 128, 1311, 1392 |
| Bsh1236I | CGCG | 3 cut(s) | 2099, 3093, 3588 |
| BsiHKAI | GWGCWC | 4 cut(s) | 95, 3457, 3609, 3695 |
| BsiHKCI | CYCGRG | 2 cut(s) | 306, 3402 |
| BsiSI | CCGG | 4 cut(s) | 671, 1918, 2295, 2745 |
| BslFI | GGGAC | 4 cut(s) | 323, 604, 2932, 3304 |
| BsmAI | GTCTC | 7 cut(s) | 516, 987, 1640, 1931, 2256, 2397, 4324 |
| BsmBI | CGTCTC | 1 cut(s) | 2256 |
| BsmFI | GGGAC | 4 cut(s) | 323, 604, 2932, 3304 |
| BsmI | GAATGC | 3 cut(s) | 899, 945, 3053 |
| Bso31I | GGTCTC | 1 cut(s) | 4324 |
| BsoBI | CYCGRG | 2 cut(s) | 306, 3402 |
| Bsp120I | GGGCCC | 1 cut(s) | 795 |
| Bsp1286I | GDGCHC | 8 cut(s) | 95, 799, 859, 1375, 3457, 3609, 3695, 3954 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp19I | CCATGG | 1 cut(s) | 1498 |
| BspFNI | CGCG | 3 cut(s) | 2099, 3093, 3588 |
| BspHI | TCATGA | 1 cut(s) | 402 |
| BspMAI | CTGCAG | 3 cut(s) | 598, 924, 4142 |
| BspMI | ACCTGC | 6 cut(s) | 793, 1027, 1843, 2209, 2636, 3302 |
| BspOI | GCTAGC | 1 cut(s) | 3882 |
| BspPI | GGATC | 6 cut(s) | 819, 2418, 2431, 3068, 4313, 4326 |
| BspTI | CTTAAG | 1 cut(s) | 1769 |
| BspTNI | GGTCTC | 1 cut(s) | 4324 |
| BsrBI | CCGCTC | 2 cut(s) | 228, 2916 |
| BsrDI | GCAATG | 3 cut(s) | 270, 612, 627 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BsrI | ACTGG | 9 cut(s) | 1067, 2110, 2470, 2977, 3189, 3341, 3564, 3879, 4199 |
| BssECI | CCNNGG | 4 cut(s) | 1242, 1498, 2295, 2471 |
| BssNI | GRCGYC | 1 cut(s) | 237 |
| BssSI | CACGAG | 1 cut(s) | 2065 |
| BssT1I | CCWWGG | 2 cut(s) | 1242, 1498 |
| Bst2BI | CACGAG | 1 cut(s) | 2065 |
| Bst2UI | CCWGG | 2 cut(s) | 2926, 3057 |
| Bst6I | CTCTTC | 4 cut(s) | 360, 2345, 3201, 3640 |
| BstACI | GRCGYC | 1 cut(s) | 237 |
| BstAFI | CTTAAG | 1 cut(s) | 1769 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 602, 1759, 3246, 3506 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstBAI | YACGTR | 1 cut(s) | 1426 |
| BstDSI | CCRYGG | 1 cut(s) | 1498 |
| BstEII | GGTNACC | 2 cut(s) | 517, 2018 |
| BstFNI | CGCG | 3 cut(s) | 2099, 3093, 3588 |
| BstH2I | RGCGCY | 1 cut(s) | 727 |
| BstHHI | GCGC | 5 cut(s) | 726, 2101, 2224, 3093, 3590 |
| BstMAI | GTCTC | 7 cut(s) | 516, 987, 1640, 1931, 2256, 2397, 4324 |
| BstNI | CCWGG | 2 cut(s) | 2926, 3057 |
| BstNSI | RCATGY | 2 cut(s) | 1000, 1295 |
| BstPI | GGTNACC | 2 cut(s) | 517, 2018 |
| BstSCI | CCNGG | 3 cut(s) | 2294, 2924, 3055 |
| BstSFI | CTRYAG | 6 cut(s) | 99, 594, 790, 920, 2679, 4138 |
| BstSLI | GKGCMC | 2 cut(s) | 799, 1375 |
| BstUI | CGCG | 3 cut(s) | 2099, 3093, 3588 |
| BstV2I | GAAGAC | 2 cut(s) | 765, 2752 |
| BstX2I | RGATCY | 6 cut(s) | 133, 811, 924, 2423, 3073, 4318 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3571 |
| BstYI | RGATCY | 6 cut(s) | 133, 811, 924, 2423, 3073, 4318 |
| BtgI | CCRYGG | 1 cut(s) | 1498 |
| BtsI | GCAGTG | 1 cut(s) | 2256 |
| BveI | ACCTGC | 6 cut(s) | 793, 1027, 1843, 2209, 2636, 3302 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3012 |
| CciI | TCATGA | 1 cut(s) | 402 |
| CfoI | GCGC | 5 cut(s) | 726, 2101, 2224, 3093, 3590 |
| CseI | GACGC | 2 cut(s) | 1786, 2174 |
| Csp6I | GTAC | 6 cut(s) | 4, 524, 591, 2649, 3958, 4385 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 3687, 3722 |
| CviQI | GTAC | 6 cut(s) | 4, 524, 591, 2649, 3958, 4385 |
| DraI | TTTAAA | 1 cut(s) | 2638 |
| DraIII | CACNNNGTG | 1 cut(s) | 1271 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1991, 2926 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1991, 2926 |
| Eam1104I | CTCTTC | 4 cut(s) | 360, 2345, 3201, 3640 |
| EarI | CTCTTC | 4 cut(s) | 360, 2345, 3201, 3640 |
| Ecl136II | GAGCTC | 2 cut(s) | 3607, 3693 |
| Eco130I | CCWWGG | 2 cut(s) | 1242, 1498 |
| Eco147I | AGGCCT | 1 cut(s) | 3904 |
| Eco24I | GRGCYC | 5 cut(s) | 799, 859, 3609, 3695, 3954 |
| Eco31I | GGTCTC | 1 cut(s) | 4324 |
| Eco32I | GATATC | 1 cut(s) | 1030 |
| Eco47I | GGWCC | 5 cut(s) | 310, 565, 1281, 2928, 3291 |
| Eco47III | AGCGCT | 1 cut(s) | 725 |
| Eco53kI | GAGCTC | 2 cut(s) | 3607, 3693 |
| Eco57I | CTGAAG | 5 cut(s) | 562, 759, 986, 1179, 1671 |
| Eco72I | CACGTG | 1 cut(s) | 1426 |
| Eco88I | CYCGRG | 2 cut(s) | 306, 3402 |
| Eco91I | GGTNACC | 2 cut(s) | 517, 2018 |
| EcoICRI | GAGCTC | 2 cut(s) | 3607, 3693 |
| EcoO109I | RGGNCCY | 5 cut(s) | 310, 565, 795, 796, 3291 |
| EcoO65I | GGTNACC | 2 cut(s) | 517, 2018 |
| EcoRI | GAATTC | 2 cut(s) | 1452, 3752 |
| EcoRII | CCWGG | 2 cut(s) | 2924, 3055 |
| EcoRV | GATATC | 1 cut(s) | 1030 |
| EcoT14I | CCWWGG | 2 cut(s) | 1242, 1498 |
| EcoT22I | ATGCAT | 2 cut(s) | 1561, 3165 |
| EcoT38I | GRGCYC | 5 cut(s) | 799, 859, 3609, 3695, 3954 |
| ErhI | CCWWGG | 2 cut(s) | 1242, 1498 |
| Esp3I | CGTCTC | 1 cut(s) | 2256 |
| FaqI | GGGAC | 4 cut(s) | 323, 604, 2932, 3304 |
| FauI | CCCGC | 3 cut(s) | 1153, 2909, 2981 |
| FauNDI | CATATG | 3 cut(s) | 353, 851, 2354 |
| FbaI | TGATCA | 2 cut(s) | 2329, 3427 |
| FblI | GTMKAC | 1 cut(s) | 846 |
| FriOI | GRGCYC | 5 cut(s) | 799, 859, 3609, 3695, 3954 |
| FspI | TGCGCA | 1 cut(s) | 2223 |
| GlaI | GCGC | 5 cut(s) | 725, 2100, 2223, 3092, 3589 |
| GsaI | CCCAGC | 2 cut(s) | 2497, 3291 |
| GsuI | CTGGAG | 3 cut(s) | 395, 1883, 4298 |
| HaeII | RGCGCY | 1 cut(s) | 727 |
| HapII | CCGG | 4 cut(s) | 671, 1918, 2295, 2745 |
| HgaI | GACGC | 2 cut(s) | 1786, 2174 |
| HhaI | GCGC | 5 cut(s) | 726, 2101, 2224, 3093, 3590 |
| Hin1I | GRCGYC | 1 cut(s) | 237 |
| Hin6I | GCGC | 5 cut(s) | 724, 2099, 2222, 3091, 3588 |
| HinP1I | GCGC | 5 cut(s) | 724, 2099, 2222, 3091, 3588 |
| HpaII | CCGG | 4 cut(s) | 671, 1918, 2295, 2745 |
| Hpy99I | CGWCG | 2 cut(s) | 242, 976 |
| HpyCH4IV | ACGT | 3 cut(s) | 237, 1229, 1425 |
| HpySE526I | ACGT | 3 cut(s) | 237, 1229, 1425 |
| Hsp92I | GRCGYC | 1 cut(s) | 237 |
| HspAI | GCGC | 5 cut(s) | 724, 2099, 2222, 3091, 3588 |
| Ksp22I | TGATCA | 2 cut(s) | 2329, 3427 |
| MaeII | ACGT | 3 cut(s) | 237, 1229, 1425 |
| MbiI | CCGCTC | 2 cut(s) | 228, 2916 |
| MfeI | CAATTG | 1 cut(s) | 3504 |
| MflI | RGATCY | 6 cut(s) | 133, 811, 924, 2423, 3073, 4318 |
| MhlI | GDGCHC | 8 cut(s) | 95, 799, 859, 1375, 3457, 3609, 3695, 3954 |
| MlyI | GAGTC | 6 cut(s) | 920, 2035, 2057, 2374, 2454, 2777 |
| MmeI | TCCRAC | 4 cut(s) | 220, 2544, 3702, 4104 |
| Mph1103I | ATGCAT | 2 cut(s) | 1561, 3165 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1795, 2146, 3051 |
| MseI | TTAA | 6 cut(s) | 1053, 1563, 1770, 2505, 2637, 4226 |
| MslI | CAYNNNNRTG | 7 cut(s) | 426, 1037, 1440, 1446, 1554, 2781, 3120 |
| MspA1I | CMGCKG | 3 cut(s) | 212, 1675, 2729 |
| MspCI | CTTAAG | 1 cut(s) | 1769 |
| MspI | CCGG | 4 cut(s) | 671, 1918, 2295, 2745 |
| MspR9I | CCNGG | 3 cut(s) | 2296, 2926, 3057 |
| MunI | CAATTG | 1 cut(s) | 3504 |
| Mva1269I | GAATGC | 3 cut(s) | 899, 945, 3053 |
| MvaI | CCWGG | 2 cut(s) | 2926, 3057 |
| MvnI | CGCG | 3 cut(s) | 2099, 3093, 3588 |
| NciI | CCSGG | 1 cut(s) | 2296 |
| NcoI | CCATGG | 1 cut(s) | 1498 |
| NdeI | CATATG | 3 cut(s) | 353, 851, 2354 |
| NheI | GCTAGC | 1 cut(s) | 3878 |
| NmuCI | GTSAC | 6 cut(s) | 433, 503, 1830, 2018, 2162, 3995 |
| NsbI | TGCGCA | 1 cut(s) | 2223 |
| NsiI | ATGCAT | 2 cut(s) | 1561, 3165 |
| NspI | RCATGY | 2 cut(s) | 1000, 1295 |
| OliI | CACNNNNGTG | 2 cut(s) | 1037, 3120 |
| PaeI | GCATGC | 1 cut(s) | 1295 |
| PaeR7I | CTCGAG | 1 cut(s) | 3402 |
| PagI | TCATGA | 1 cut(s) | 402 |
| PaqCI | CACCTGC | 3 cut(s) | 793, 1027, 1843 |
| PceI | AGGCCT | 1 cut(s) | 3904 |
| PctI | GAATGC | 3 cut(s) | 899, 945, 3053 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1795, 2146, 3051 |
| PflFI | GACNNNGTC | 2 cut(s) | 314, 1942 |
| PleI | GAGTC | 6 cut(s) | 919, 2035, 2057, 2374, 2454, 2776 |
| PmaCI | CACGTG | 1 cut(s) | 1426 |
| PmlI | CACGTG | 1 cut(s) | 1426 |
| PpsI | GAGTC | 6 cut(s) | 919, 2035, 2057, 2374, 2454, 2776 |
| Ppu21I | YACGTR | 1 cut(s) | 1426 |
| PpuMI | RGGWCCY | 3 cut(s) | 310, 565, 3291 |
| PshBI | ATTAAT | 1 cut(s) | 2505 |
| Psp124BI | GAGCTC | 2 cut(s) | 3609, 3695 |
| Psp5II | RGGWCCY | 3 cut(s) | 310, 565, 3291 |
| Psp6I | CCWGG | 2 cut(s) | 2924, 3055 |
| PspCI | CACGTG | 1 cut(s) | 1426 |
| PspEI | GGTNACC | 2 cut(s) | 517, 2018 |
| PspFI | CCCAGC | 2 cut(s) | 2493, 3287 |
| PspGI | CCWGG | 2 cut(s) | 2924, 3055 |
| PspOMI | GGGCCC | 1 cut(s) | 795 |
| PspPPI | RGGWCCY | 3 cut(s) | 310, 565, 3291 |
| PstI | CTGCAG | 3 cut(s) | 598, 924, 4142 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3012 |
| PsuI | RGATCY | 6 cut(s) | 133, 811, 924, 2423, 3073, 4318 |
| PsyI | GACNNNGTC | 2 cut(s) | 314, 1942 |
| PvuII | CAGCTG | 3 cut(s) | 212, 1675, 2729 |
| RsaI | GTAC | 6 cut(s) | 5, 525, 592, 2650, 3959, 4386 |
| RsaNI | GTAC | 6 cut(s) | 4, 524, 591, 2649, 3958, 4385 |
| RseI | CAYNNNNRTG | 7 cut(s) | 426, 1037, 1440, 1446, 1554, 2781, 3120 |
| SacI | GAGCTC | 2 cut(s) | 3609, 3695 |
| SaqAI | TTAA | 6 cut(s) | 1053, 1563, 1770, 2505, 2637, 4226 |
| SchI | GAGTC | 6 cut(s) | 920, 2035, 2057, 2374, 2454, 2777 |
| ScrFI | CCNGG | 3 cut(s) | 2296, 2926, 3057 |
| SduI | GDGCHC | 8 cut(s) | 95, 799, 859, 1375, 3457, 3609, 3695, 3954 |
| SfcI | CTRYAG | 6 cut(s) | 99, 594, 790, 920, 2679, 4138 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 222 |
| Sfr274I | CTCGAG | 1 cut(s) | 3402 |
| SinI | GGWCC | 5 cut(s) | 310, 565, 1281, 2928, 3291 |
| SlaI | CTCGAG | 1 cut(s) | 3402 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 426, 1037, 1440, 1446, 1554, 2781, 3120 |
| SmlI | CTYRAG | 6 cut(s) | 248, 1769, 2043, 2556, 2654, 3402 |
| SmoI | CTYRAG | 6 cut(s) | 248, 1769, 2043, 2556, 2654, 3402 |
| SpeI | ACTAGT | 1 cut(s) | 1541 |
| SphI | GCATGC | 1 cut(s) | 1295 |
| SseBI | AGGCCT | 1 cut(s) | 3904 |
| SspI | AATATT | 3 cut(s) | 500, 2146, 2236 |
| SstI | GAGCTC | 2 cut(s) | 3609, 3695 |
| StuI | AGGCCT | 1 cut(s) | 3904 |
| StyD4I | CCNGG | 3 cut(s) | 2294, 2924, 3055 |
| StyI | CCWWGG | 2 cut(s) | 1242, 1498 |
| TaiI | ACGT | 3 cut(s) | 240, 1232, 1428 |
| TatI | WGTACW | 3 cut(s) | 3, 590, 3957 |
| TauI | GCSGC | 6 cut(s) | 228, 471, 1787, 2099, 2988, 3096 |
| Tru1I | TTAA | 6 cut(s) | 1053, 1563, 1770, 2505, 2637, 4226 |
| Tru9I | TTAA | 6 cut(s) | 1053, 1563, 1770, 2505, 2637, 4226 |
| TseFI | GTSAC | 6 cut(s) | 433, 503, 1830, 2018, 2162, 3995 |
| Tsp45I | GTSAC | 6 cut(s) | 433, 503, 1830, 2018, 2162, 3995 |
| TspGWI | ACGGA | 3 cut(s) | 111, 388, 1997 |
| Tth111I | GACNNNGTC | 2 cut(s) | 314, 1942 |
| Vha464I | CTTAAG | 1 cut(s) | 1769 |
| VpaK11BI | GGWCC | 5 cut(s) | 310, 565, 1281, 2928, 3291 |
| VspI | ATTAAT | 1 cut(s) | 2505 |
| XapI | RAATTY | 6 cut(s) | 12, 1452, 1474, 1580, 1715, 3752 |
| XceI | RCATGY | 2 cut(s) | 1000, 1295 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 460, 3180, 3932 |
| XhoI | CTCGAG | 1 cut(s) | 3402 |
| XmiI | GTMKAC | 1 cut(s) | 846 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1795, 2146, 3051 |
| ZraI | GACGTC | 1 cut(s) | 238 |
| Zsp2I | ATGCAT | 2 cut(s) | 1561, 3165 |