Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0001175

Physical Location & Seq

Genomic Coordinates Forward (+)

66188517 .. 66197344

Gene Structure 8828 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00332160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGCTGGAGAAGCAAAGTTACTTGCAGTTCATTTTGGTATTGGAGGCGGTTTCTGAGGAGGTTCATGGTAGCGCTAAAGTTCCAGAGTCAGATATAGCTCAAGCATGCATAAAAGTAAGTGCTTCAGTTGATTTCAACTTCCCTTACCTAAACTCTACTTTTGGTGGAATCTGGTTGTCTATTGATGTTCCAAGCATGCCAGCAAACGACACGGCGTTGGCAAAAGTTACAGCGACGTGCTCAGGTGCACATCCTCTAAAGCTACGGCAGGAACCCATCCGTCCAGCCCTGGTGCTATATAGGAGCAGCCATATCTCCATTTGCCGCCCCCACCTTGATTTCTCTTTCATAATGTGGAGGTTCAAGCCTTTTATGCACAAGGAGCCAGCTGGCCTCGAAGGCCGCTCCATTGACGTCGGAACTCTCAAGATTCAGGTGAAGAATGTCATTGCCGAAGGAGGGTTCTCTTGCGTTTACTTAGCTCGGGACCTTGTCCATACCTCAAAGCAGTATGCTTTGAAGCACATCATATGTAATGATGAAGAGTTGCTGGAGTTGGTGATGAAGGAAATCTCCGTCATGAAGTTACTCAGAGGGCATCCCAATGTTGTCACTCTCTATGCCCACGCCATCATGGATTTGGGCCGCACAAAGGAGGCACTCCTTGTCATGGAATATTGTGACAAGTCTCTGGTTACCGTACTCGATAGCAGAGGAGCTGGCTTTTTTGATGAGAAGCAGGTCCTTGCAATCTTCAGAGATGTGTGTACTGCAGTTTTTGCAATGCACACACTGTCCCCTCCCATTGCTCATAGGGATTTGAAAGCTGAGAATCTTCTGTTGGGGCCGGATGGACTGTGGAAATTGTGTGATTTTGGAAGCACCTCTACCAACCACAAGCGCTTTGAGAAGCCTGAAGAAATGGGTATTGAAGAAGACATTATCAGAAAGCACACAACACCTGCCTACAGGGCCCCTGAGGCTCTTGGCTGCCTTCTCTTTCGCATTTGCTACTTCAAAAGTGCATTCGATGGAGAGTCAAAGCTGCAGATCTTGAATGGAAACTATCGCATTCCTGAGTTACCTAAATACGGCTCGTCGATTACAGATCTCATCAGAGACATGCTTCAGGCATCACCAGACGACAGACCAGATATCACGCAGGCAAGCGCTTTGCTTGATTGGCCATTCATCTCCATGAATTTAGTTGGTTTACAGAAGTCTTTACCTGATAGGCCACCTGAAATGCACTCTACTGGAAAACATGAAGGTTTTTCAAGGCCCGCAAACAAGTCGCCTGAAATGCCCCGTAGAAGCCCACCCCCACCACCTTCAGTGGGAGAATCAGCTAGAAATCCATCACAACCGTCAAATATATCCAAGGCAGGGGGAGGTGGAGGGCCACTTGGTGCATTCTGGTCCACTCAGCATGCTGAGGATTCAGTTGTTGCTGAAGAAAAGACTGTACCTAAGTTTGATGATTCTAATCATCCTTTACCCAAGAACAGTGCCCCCGCAAAAGTGGAAAATACACCAACCTATGCTGCACGAAGAAACTCACACATGAAATCGCACAAGCATGAAGATGGATTTCCAAAGGACATTGAAATAAATTTCTTCCAGAAGGATACTGACCATGGTGCTGAGAGGCCAAAGGCATCCAAGCCTGAGAGTGCAACTAGTTTTCACGATGATGCATTTAACTCGTTTGTTGCTGAATTTGATACTAATAAACTCAGCGTGGGAGCTAGTAATAGCAAATCAGGAAAAGAAGGAGCATTAGAGACTGAAGTAGAGAGATTGAAGGAGCAGCTGAACCAAGCCAACATAGAAAAGGCTGAAATAACTTCCAAATTTGAAAAGCTATCAGCCATCTGCCGATCTCAAAGGCAAGAGTTGCAGGAGCTTAAGCAGACGCTTGCTGCTAGAACACCTTCACCGAATAAAGATGCTTTGAGAAATCAAATGTCACCTGGCATTCAGTCATCCGCTACCCCACCGCAGAGGGAGAAGATAGGAGGAGCCATTTGGGAACTCCAGCAGGATAAATCTGACCAGAAAACTCCGAGTCCAGAGACAAAGTCATGGCAGCCGTTTGCAGAACCGCAGCCTTCTTCAACGGACAACACTGTCAAATCTGTCAGGACAAGAAATGGTCACCAGAACAAGCAAGCTACGACTCAAGTAAATACTGGTTTTGACTCGTGGGGTTTTGGAACAGAGAGCTTTTCTGCTGCGCCCACTGGAAGCACCCAAATATCAAGACCTATGGGTGAAGGAAATACTTCTCAGCGTTTTGGTGACGCAAAGGCTATGGAGAGCAAGCCAGCTTCTCAACCTGCTGGATGGGCTGGTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030100 GO:0032879 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0060627 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

786

Amino Acids

86.74

Weight (kDa)

6.66

Isoelectric Point (pI)

53.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	144 - 387	7.8e-16	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	145 - 389	2e-30	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32850 AT2G32850
fragaria_vesca	FvH4_7g00200 FvH4_7g00210 FvH4_7g00210
malus_domestica	MD02G1318100.v1.1 MD14G1085700.v1.1 MD14G1085800.v1.1
prunus_persica	Prupe.2G004700_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1 Prupe.2G004800_v2.0.a1
pyrus_communis	pycom02g26640 pycom14g07560
rosa_chinensis	RchiOBHm_Chr1g0313461 RchiOBHm_Chr1g0313481 RchiOBHm_Chr1g0313501 RchiOBHm_Chr1g0313511 RchiOBHm_Chr1g0313531 RchiOBHm_Chr1g0313561 RchiOBHm_Chr1g0313571 RchiOBHm_Chr1g0313591 RchiOBHm_Chr1g0313601 RchiOBHm_Chr1g0313611 RchiOBHm_Chr1g0313621 RchiOBHm_Chr1g0313631 RchiOBHm_Chr1g0372901
rosa_laevigata	RLG00000026867 RLG00000030789 RLG00000030790 RLG00000030791
rosa_multiflora	Rmu_co8009002.1_g000001 Rmu_sc0000931.1_g000014 Rmu_sc0000931.1_g000018 Rmu_sc0015122.1_g000001
rosa_roxburghii	Rroxscaffold_4G00284640 Rroxscaffold_4G00332160
rosa_rugosa	Rorug01G0374800 RorugPtG0005200.1 RorugPtG0005300.1 RorugPtG0005400.1
rosa_samantha	Rh1AG003900 Rh1AG005500 Rh1BG000900 Rh1CG000500 Rh1CG000700 Rh1CG008400 Rh1DG003600 Rh1DG003700 Rh1DG003800 Rh1DG378100
rosa_wichuraiana	Rw1G000310 Rw1G000320 Rw1G000330 Rw1G033770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	970
AasI	GACNNNNNNGTC	1 cut(s)	2129
AatII	GACGTC	1 cut(s)	417
Acc36I	ACCTGC	3 cut(s)	730, 970, 2347
AccBSI	CCGCTC	1 cut(s)	405
AciI	CCGC	9 cut(s)	47, 325, 403, 646, 1284, 1515, 1989, 2000, 2105
AcoI	YGGCCR	1 cut(s)	1184
AcsI	RAATTY	4 cut(s)	1201, 1612, 1718, 1853
AcuI	CTGAAG	7 cut(s)	108, 739, 936, 1112, 1317, 1473, 1809
AcyI	GRCGYC	1 cut(s)	414
AdeI	CACNNNGTG	1 cut(s)	1409
AfaI	GTAC	3 cut(s)	702, 769, 1467
AfeI	AGCGCT	3 cut(s)	73, 902, 1171
AfiI	CCNNNNNNNGG	6 cut(s)	459, 652, 670, 1091, 1337, 1386
AflII	CTTAAG	1 cut(s)	1907
AhlI	ACTAGT	1 cut(s)	1679
AjiI	CACGTC	1 cut(s)	237
AjnI	CCWGG	2 cut(s)	288, 1972
AjuI	GAANNNNNNNTTGG	2 cut(s)	824, 856
Alw21I	GWGCWC	2 cut(s)	242, 250
Alw26I	GTCTC	4 cut(s)	693, 1113, 1778, 2069
Alw44I	GTGCAC	1 cut(s)	246
Ama87I	CYCGRG	1 cut(s)	483
Aor51HI	AGCGCT	3 cut(s)	73, 902, 1171
ApaI	GGGCCC	1 cut(s)	976
ApaLI	GTGCAC	1 cut(s)	246
ApeKI	GCWGC	9 cut(s)	306, 990, 1045, 1544, 1810, 1922, 2089, 2107, 2234
ApoI	RAATTY	4 cut(s)	1201, 1612, 1718, 1853
Asp700I	GAANNNNTTC	2 cut(s)	1933, 2284
AspLEI	GCGC	4 cut(s)	74, 903, 1172, 2239
AspS9I	GGNCC	9 cut(s)	487, 643, 742, 845, 972, 973, 1281, 1400, 1419
AsuHPI	GGTGA	8 cut(s)	448, 571, 1128, 1929, 1962, 2150, 2285, 2312
AvaI	CYCGRG	1 cut(s)	483
AvaII	GGWCC	3 cut(s)	487, 742, 1419
AxyI	CCTNAGG	1 cut(s)	978
BaeGI	GKGCMC	3 cut(s)	250, 976, 1513
BalI	TGGCCA	1 cut(s)	1186
BanII	GRGCYC	1 cut(s)	976
BarI	GAAGNNNNNNTAC	3 cut(s)	34, 871, 903
BauI	CACGAG	1 cut(s)	2203
BbsI	GAAGAC	1 cut(s)	942
Bbv12I	GWGCWC	2 cut(s)	242, 250
BbvCI	CCTCAGC	1 cut(s)	1434
BbvI	GCAGC	9 cut(s)	318, 977, 1032, 1531, 1822, 1909, 2101, 2119, 2221
BccI	CCATC	8 cut(s)	284, 638, 845, 1025, 1366, 1580, 1880, 2340
BceAI	ACGGC	4 cut(s)	228, 281, 1108, 2077
BcgI	CGANNNNNNTGC	4 cut(s)	1275, 1309, 1686, 1720
BciT130I	CCWGG	2 cut(s)	290, 1974
BciVI	GTATCC	1 cut(s)	1621
BcoDI	GTCTC	4 cut(s)	693, 1113, 1778, 2069
BcuI	ACTAGT	1 cut(s)	1679
BfaI	CTAG	4 cut(s)	1350, 1680, 1749, 1926
BfmI	CTRYAG	3 cut(s)	771, 967, 1046
BfoI	RGCGCY	3 cut(s)	75, 904, 1173
BfrI	CTTAAG	1 cut(s)	1907
BfuAI	ACCTGC	3 cut(s)	730, 970, 2347
BfuI	GTATCC	1 cut(s)	1621
BglI	GCCNNNNNGGC	3 cut(s)	399, 971, 980
BglII	AGATCT	2 cut(s)	1050, 1108
Bme1390I	CCNGG	2 cut(s)	290, 1974
Bme18I	GGWCC	3 cut(s)	487, 742, 1419
BmeT110I	CYCGRG	1 cut(s)	483
BmgBI	CACGTC	1 cut(s)	237
BmgT120I	GGNCC	9 cut(s)	487, 643, 742, 845, 972, 973, 1281, 1400, 1419
BmiI	GGNNCC	7 cut(s)	273, 384, 488, 846, 974, 975, 2023
BmrFI	CCNGG	2 cut(s)	290, 1974
BmsI	GCATC	5 cut(s)	607, 1142, 1667, 1684, 1939
BpiI	GAAGAC	1 cut(s)	942
BpmI	CTGGAG	3 cut(s)	26, 572, 2021
Bpu10I	CCTNAGC	2 cut(s)	241, 1434
BpuEI	CTTGAG	3 cut(s)	84, 410, 2166
BsaBI	GATNNNNATC	1 cut(s)	1485
BsaHI	GRCGYC	1 cut(s)	414
BsaJI	CCNNGG	3 cut(s)	288, 1380, 1636
Bsc4I	CCNNNNNNNGG	6 cut(s)	459, 652, 670, 1091, 1337, 1386
Bse1I	ACTGG	3 cut(s)	1261, 2197, 2248
Bse21I	CCTNAGG	1 cut(s)	978
Bse3DI	GCAATG	3 cut(s)	447, 789, 804
Bse8I	GATNNNNATC	1 cut(s)	1485
BseBI	CCWGG	2 cut(s)	290, 1974
BseDI	CCNNGG	3 cut(s)	288, 1380, 1636
BseGI	GGATG	8 cut(s)	250, 276, 598, 856, 1489, 1658, 1985, 2351
BseJI	GATNNNNATC	1 cut(s)	1485
BseLI	CCNNNNNNNGG	6 cut(s)	459, 652, 670, 1091, 1337, 1386
BseMI	GCAATG	3 cut(s)	447, 789, 804
BseNI	ACTGG	3 cut(s)	1261, 2197, 2248
BseRI	GAGGAG	3 cut(s)	71, 729, 2034
BseSI	GKGCMC	3 cut(s)	250, 976, 1513
BseXI	GCAGC	9 cut(s)	318, 977, 1032, 1531, 1822, 1909, 2101, 2119, 2221
BsgI	GTGCAG	1 cut(s)	1530
BsiHKAI	GWGCWC	2 cut(s)	242, 250
BsiHKCI	CYCGRG	1 cut(s)	483
BsiSI	CCGG	1 cut(s)	848
BslFI	GGGAC	2 cut(s)	500, 781
BslI	CCNNNNNNNGG	6 cut(s)	459, 652, 670, 1091, 1337, 1386
BsmAI	GTCTC	4 cut(s)	693, 1113, 1778, 2069
BsmFI	GGGAC	2 cut(s)	500, 781
BsmI	GAATGC	4 cut(s)	1025, 1071, 1412, 1977
BsoBI	CYCGRG	1 cut(s)	483
Bsp120I	GGGCCC	1 cut(s)	972
Bsp1286I	GDGCHC	4 cut(s)	242, 250, 976, 1513
Bsp143I	GATC	3 cut(s)	1050, 1108, 1880
Bsp19I	CCATGG	1 cut(s)	1636
BspACI	CCGC	9 cut(s)	47, 325, 403, 646, 1284, 1515, 1989, 2000, 2105
BspHI	TCATGA	1 cut(s)	579
BspLI	GGNNCC	7 cut(s)	273, 384, 488, 846, 974, 975, 2023
BspMAI	CTGCAG	2 cut(s)	775, 1050
BspMI	ACCTGC	3 cut(s)	730, 970, 2347
BspTI	CTTAAG	1 cut(s)	1907
BsrBI	CCGCTC	1 cut(s)	405
BsrDI	GCAATG	3 cut(s)	447, 789, 804
BsrI	ACTGG	3 cut(s)	1261, 2197, 2248
BssECI	CCNNGG	3 cut(s)	288, 1380, 1636
BssMI	GATC	3 cut(s)	1050, 1108, 1880
BssNI	GRCGYC	1 cut(s)	414
BssSI	CACGAG	1 cut(s)	2203
BssT1I	CCWWGG	2 cut(s)	1380, 1636
Bst2BI	CACGAG	1 cut(s)	2203
Bst2UI	CCWGG	2 cut(s)	290, 1974
Bst4CI	ACNGT	7 cut(s)	700, 795, 858, 1368, 1465, 1508, 2131
Bst6I	CTCTTC	1 cut(s)	537
BstACI	GRCGYC	1 cut(s)	414
BstAFI	CTTAAG	1 cut(s)	1907
BstAPI	GCANNNNNTGC	2 cut(s)	779, 1897
BstDSI	CCRYGG	1 cut(s)	1636
BstEII	GGTNACC	2 cut(s)	694, 2156
BstF5I	GGATG	8 cut(s)	250, 276, 598, 856, 1489, 1658, 1985, 2351
BstH2I	RGCGCY	3 cut(s)	75, 904, 1173
BstHHI	GCGC	4 cut(s)	74, 903, 1172, 2239
BstKTI	GATC	3 cut(s)	1053, 1111, 1883
BstMAI	GTCTC	4 cut(s)	693, 1113, 1778, 2069
BstMBI	GATC	3 cut(s)	1050, 1108, 1880
BstNI	CCWGG	2 cut(s)	290, 1974
BstNSI	RCATGY	4 cut(s)	108, 199, 1126, 1433
BstPI	GGTNACC	2 cut(s)	694, 2156
BstSCI	CCNGG	2 cut(s)	288, 1972
BstSFI	CTRYAG	3 cut(s)	771, 967, 1046
BstSLI	GKGCMC	3 cut(s)	250, 976, 1513
BstV1I	GCAGC	9 cut(s)	318, 977, 1032, 1531, 1822, 1909, 2101, 2119, 2221
BstV2I	GAAGAC	1 cut(s)	942
BstX2I	RGATCY	2 cut(s)	1050, 1108
BstYI	RGATCY	2 cut(s)	1050, 1108
Bsu36I	CCTNAGG	1 cut(s)	978
BsuI	GTATCC	1 cut(s)	1621
BtgI	CCRYGG	1 cut(s)	1636
BtrI	CACGTC	1 cut(s)	237
BtsCI	GGATG	8 cut(s)	250, 276, 598, 856, 1489, 1658, 1985, 2351
BtsIMutI	CAGTG	5 cut(s)	791, 1341, 1513, 2127, 2241
BveI	ACCTGC	3 cut(s)	730, 970, 2347
CciI	TCATGA	1 cut(s)	579
CfoI	GCGC	4 cut(s)	74, 903, 1172, 2239
Cfr13I	GGNCC	9 cut(s)	487, 643, 742, 845, 972, 973, 1281, 1400, 1419
CseI	GACGC	2 cut(s)	1924, 2312
Csp6I	GTAC	3 cut(s)	701, 768, 1466
CviQI	GTAC	3 cut(s)	701, 768, 1466
DpnI	GATC	3 cut(s)	1052, 1110, 1882
DpnII	GATC	3 cut(s)	1050, 1108, 1880
DraIII	CACNNNGTG	1 cut(s)	1409
DrdI	GACNNNNNNGTC	1 cut(s)	2129
DseDI	GACNNNNNNGTC	1 cut(s)	2129
EaeI	YGGCCR	1 cut(s)	1184
Eam1104I	CTCTTC	1 cut(s)	537
EarI	CTCTTC	1 cut(s)	537
Eco130I	CCWWGG	2 cut(s)	1380, 1636
Eco24I	GRGCYC	1 cut(s)	976
Eco32I	GATATC	1 cut(s)	1156
Eco47I	GGWCC	3 cut(s)	487, 742, 1419
Eco47III	AGCGCT	3 cut(s)	73, 902, 1171
Eco57I	CTGAAG	7 cut(s)	108, 739, 936, 1112, 1317, 1473, 1809
Eco81I	CCTNAGG	1 cut(s)	978
Eco88I	CYCGRG	1 cut(s)	483
Eco91I	GGTNACC	2 cut(s)	694, 2156
EcoO109I	RGGNCCY	4 cut(s)	487, 742, 972, 973
EcoO65I	GGTNACC	2 cut(s)	694, 2156
EcoRII	CCWGG	2 cut(s)	288, 1972
EcoRV	GATATC	1 cut(s)	1156
EcoT14I	CCWWGG	2 cut(s)	1380, 1636
EcoT22I	ATGCAT	2 cut(s)	110, 1699
EcoT38I	GRGCYC	1 cut(s)	976
ErhI	CCWWGG	2 cut(s)	1380, 1636
FaqI	GGGAC	2 cut(s)	500, 781
FauI	CCCGC	2 cut(s)	1291, 1522
FauNDI	CATATG	1 cut(s)	530
FokI	GGATG	7 cut(s)	237, 263, 585, 863, 1476, 1645, 1972
FriOI	GRGCYC	1 cut(s)	976
FspBI	CTAG	4 cut(s)	1350, 1680, 1749, 1926
GlaI	GCGC	4 cut(s)	73, 902, 1171, 2238
GsuI	CTGGAG	3 cut(s)	26, 572, 2021
HaeII	RGCGCY	3 cut(s)	75, 904, 1173
HapII	CCGG	1 cut(s)	848
HgaI	GACGC	2 cut(s)	1924, 2312
HhaI	GCGC	4 cut(s)	74, 903, 1172, 2239
Hin1I	GRCGYC	1 cut(s)	414
Hin6I	GCGC	4 cut(s)	72, 901, 1170, 2237
HinP1I	GCGC	4 cut(s)	72, 901, 1170, 2237
HpaII	CCGG	1 cut(s)	848
HphI	GGTGA	8 cut(s)	448, 571, 1128, 1929, 1962, 2150, 2285, 2312
Hpy166II	GTNNAC	5 cut(s)	248, 475, 768, 1214, 1422
Hpy188I	TCNGA	9 cut(s)	55, 91, 419, 593, 758, 947, 1118, 2053, 2067
Hpy8I	GTNNAC	5 cut(s)	248, 475, 768, 1214, 1422
Hpy99I	CGWCG	3 cut(s)	238, 419, 1102
HpyCH4III	ACNGT	7 cut(s)	700, 795, 858, 1368, 1465, 1508, 2131
HpyCH4IV	ACGT	2 cut(s)	236, 414
HpySE526I	ACGT	2 cut(s)	236, 414
Hsp92I	GRCGYC	1 cut(s)	414
HspAI	GCGC	4 cut(s)	72, 901, 1170, 2237
Kzo9I	GATC	3 cut(s)	1050, 1108, 1880
LmnI	GCTCC	9 cut(s)	303, 382, 410, 716, 1745, 1775, 1807, 1903, 2021
Lsp1109I	GCAGC	9 cut(s)	318, 977, 1032, 1531, 1822, 1909, 2101, 2119, 2221
LweI	GCATC	5 cut(s)	607, 1142, 1667, 1684, 1939
MaeI	CTAG	4 cut(s)	1350, 1680, 1749, 1926
MaeII	ACGT	2 cut(s)	236, 414
MalI	GATC	3 cut(s)	1052, 1110, 1882
MbiI	CCGCTC	1 cut(s)	405
MboI	GATC	3 cut(s)	1050, 1108, 1880
MflI	RGATCY	2 cut(s)	1050, 1108
MhlI	GDGCHC	4 cut(s)	242, 250, 976, 1513
MlsI	TGGCCA	1 cut(s)	1186
MluCI	AATT	5 cut(s)	863, 1201, 1612, 1718, 1853
MluNI	TGGCCA	1 cut(s)	1186
MlyI	GAGTC	5 cut(s)	95, 1046, 2077, 2173, 2195
MmeI	TCCRAC	1 cut(s)	397
Mox20I	TGGCCA	1 cut(s)	1186
Mph1103I	ATGCAT	2 cut(s)	110, 1699
MroXI	GAANNNNTTC	2 cut(s)	1933, 2284
MscI	TGGCCA	1 cut(s)	1186
MseI	TTAA	2 cut(s)	1701, 1908
MslI	CAYNNNNRTG	5 cut(s)	603, 1196, 1578, 1584, 1692
Msp20I	TGGCCA	1 cut(s)	1186
MspA1I	CMGCKG	2 cut(s)	389, 1813
MspCI	CTTAAG	1 cut(s)	1907
MspI	CCGG	1 cut(s)	848
MspR9I	CCNGG	2 cut(s)	290, 1974
Mva1269I	GAATGC	4 cut(s)	1025, 1071, 1412, 1977
MvaI	CCWGG	2 cut(s)	290, 1974
NcoI	CCATGG	1 cut(s)	1636
NdeI	CATATG	1 cut(s)	530
NdeII	GATC	3 cut(s)	1050, 1108, 1880
NlaIV	GGNNCC	7 cut(s)	273, 384, 488, 846, 974, 975, 2023
NmuCI	GTSAC	5 cut(s)	610, 680, 1968, 2156, 2300
NsiI	ATGCAT	2 cut(s)	110, 1699
NspI	RCATGY	4 cut(s)	108, 199, 1126, 1433
PaeI	GCATGC	3 cut(s)	108, 199, 1433
PagI	TCATGA	1 cut(s)	579
PaqCI	CACCTGC	1 cut(s)	970
PctI	GAATGC	4 cut(s)	1025, 1071, 1412, 1977
PdmI	GAANNNNTTC	2 cut(s)	1933, 2284
PfeI	GAWTC	6 cut(s)	168, 430, 832, 1343, 1439, 1481
PflFI	GACNNNGTC	2 cut(s)	491, 2080
PleI	GAGTC	5 cut(s)	94, 1045, 2076, 2173, 2195
PpsI	GAGTC	5 cut(s)	94, 1045, 2076, 2173, 2195
PpuMI	RGGWCCY	2 cut(s)	487, 742
Psp5II	RGGWCCY	2 cut(s)	487, 742
Psp6I	CCWGG	2 cut(s)	288, 1972
PspEI	GGTNACC	2 cut(s)	694, 2156
PspGI	CCWGG	2 cut(s)	288, 1972
PspN4I	GGNNCC	7 cut(s)	273, 384, 488, 846, 974, 975, 2023
PspOMI	GGGCCC	1 cut(s)	972
PspPI	GGNCC	9 cut(s)	487, 643, 742, 845, 972, 973, 1281, 1400, 1419
PspPPI	RGGWCCY	2 cut(s)	487, 742
PstI	CTGCAG	2 cut(s)	775, 1050
PsuI	RGATCY	2 cut(s)	1050, 1108
PsyI	GACNNNGTC	2 cut(s)	491, 2080
PvuII	CAGCTG	2 cut(s)	389, 1813
RsaI	GTAC	3 cut(s)	702, 769, 1467
RsaNI	GTAC	3 cut(s)	701, 768, 1466
RseI	CAYNNNNRTG	5 cut(s)	603, 1196, 1578, 1584, 1692
SaqAI	TTAA	2 cut(s)	1701, 1908
Sau3AI	GATC	3 cut(s)	1050, 1108, 1880
Sau96I	GGNCC	9 cut(s)	487, 643, 742, 845, 972, 973, 1281, 1400, 1419
SchI	GAGTC	5 cut(s)	95, 1046, 2077, 2173, 2195
ScrFI	CCNGG	2 cut(s)	290, 1974
SduI	GDGCHC	4 cut(s)	242, 250, 976, 1513
SfaNI	GCATC	5 cut(s)	607, 1142, 1667, 1684, 1939
SfcI	CTRYAG	3 cut(s)	771, 967, 1046
SfiI	GGCCNNNNNGGCC	1 cut(s)	399
SinI	GGWCC	3 cut(s)	487, 742, 1419
SmiMI	CAYNNNNRTG	5 cut(s)	603, 1196, 1578, 1584, 1692
SmlI	CTYRAG	4 cut(s)	99, 425, 1907, 2181
SmoI	CTYRAG	4 cut(s)	99, 425, 1907, 2181
SpeI	ACTAGT	1 cut(s)	1679
SphI	GCATGC	3 cut(s)	108, 199, 1433
Sse9I	AATT	5 cut(s)	863, 1201, 1612, 1718, 1853
SsiI	CCGC	9 cut(s)	47, 325, 403, 646, 1284, 1515, 1989, 2000, 2105
SspI	AATATT	1 cut(s)	677
SspMI	CTAG	4 cut(s)	1350, 1680, 1749, 1926
StyD4I	CCNGG	2 cut(s)	288, 1972
StyI	CCWWGG	2 cut(s)	1380, 1636
TaaI	ACNGT	7 cut(s)	700, 795, 858, 1368, 1465, 1508, 2131
TaiI	ACGT	2 cut(s)	239, 417
TaqI	TCGA	4 cut(s)	396, 705, 1029, 1100
TasI	AATT	5 cut(s)	863, 1201, 1612, 1718, 1853
TatI	WGTACW	1 cut(s)	767
TauI	GCSGC	3 cut(s)	327, 405, 648
TfiI	GAWTC	6 cut(s)	168, 430, 832, 1343, 1439, 1481
Tru1I	TTAA	2 cut(s)	1701, 1908
Tru9I	TTAA	2 cut(s)	1701, 1908
TscAI	CASTG	5 cut(s)	798, 1341, 1513, 2134, 2248
TseFI	GTSAC	5 cut(s)	610, 680, 1968, 2156, 2300
TseI	GCWGC	9 cut(s)	306, 990, 1045, 1544, 1810, 1922, 2089, 2107, 2234
Tsp45I	GTSAC	5 cut(s)	610, 680, 1968, 2156, 2300
TspGWI	ACGGA	3 cut(s)	269, 565, 2135
TspRI	CASTG	5 cut(s)	798, 1341, 1513, 2134, 2248
Tth111I	GACNNNGTC	2 cut(s)	491, 2080
Vha464I	CTTAAG	1 cut(s)	1907
VneI	GTGCAC	1 cut(s)	246
VpaK11BI	GGWCC	3 cut(s)	487, 742, 1419
XapI	RAATTY	4 cut(s)	1201, 1612, 1718, 1853
XceI	RCATGY	4 cut(s)	108, 199, 1126, 1433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	637
XmnI	GAANNNNTTC	2 cut(s)	1933, 2284
XspI	CTAG	4 cut(s)	1350, 1680, 1749, 1926
ZraI	GACGTC	1 cut(s)	415
Zsp2I	ATGCAT	2 cut(s)	110, 1699

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.