pycom02g26640

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001175
Physical Location & Seq
Forward (+)
24448465 .. 24450432
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26640.1

Sequence Viewer

Length: 1968 bp
ATGTCTGCTTTCCTTCACTTGCTTGGCCTTTTACTTTTACTTGTAGTTGTTGAACCACTTTGTGCAGCAGCTCAATCTGCTGCAAATATTAGCTTGGGGTCGTCCCTCACCGCATCTGAAGACACATCTAAATCATCATGGCAGTCACCCTCCGGGACATTTGCTTTCGGGTTTCGCCACGTTGGTGATCAAGACACCTTCTTGCTTGCCATATGGTATGACAAAATACCAGATAAAACCATAGTTTGGTATGCAAATGGAGACGCCCCAGCACCCAAAGGATCGAAAATTGAGCTCACCGCTGATGGCCAATTCGCGCTCACCGGCCCAAAAAGCCAAGAAATCTGGAAGCCTGAGTCCGTTCTTAGTGGTGGAGTTGCGTATGCTGCCATGCTTGACACAGGCAACTTTGTGCTTGCAGGCAAGAATTCTGATTACTTATGGCAGAGCTTCAAGGATCCCGCCGACACCATTTTGCCAACCCAACTGCTGGAGATTGGCGAAAAGTTGTATTCTAGGCAGACTGCAAGCAACTACTCGAGGGGAAGGTTTCAACTCCAAGTGAAACAAGATGGAAGGCTAGTTTTATATCCGGTTGCCTTGCCTACGGAGTTCGCATACCCACCATACTATGAGAGCAACACTGCCGATGCTGCCGATGATATGAATACAGGCTTTCAGCTGCTGTTTAACGAGTCAGGTTACATCAATATTGTCACAAGGAATGGAAGTATCGTAAGCCTCACAAACAAAACAGTGTCACCAATGAGAGATTATTATTACAGAGCAACACTGGATTTTGATGGGCTTTTCACTCAATATGCTCACCCGAAATCGCCAAAAAATGGTAGCTGGACGTCCTGGTTGCCTCTCTGGTCTATCCCGGACAACATTTGCTTTGATGTAAATGGTGATTTGGGAAGTGGACCGTGTGGATACAATAGCTTCTGCAAACTGGATGCAAATAGAAGACCAATTTGTGAATGTCTTCCTGGCTTTTCCGCTTTGGATTCAAATAATAAGTTCGGTGGCTGTAAACAGAACAGAATACCGACTTGCGAACAAGGCAATTCAAAGCTAGAAGATTTGTACGTTATGCATGAGTTGACTAATACTTTTTTTCCCACTTCTGCAAACTATGAGCAAATACAGCCGATGAATGAAGATGATTGCACCAGATCTTGCCTTTACGATTGTAATTGTATGGTTGCTGTTATCAAAGAAGGCACCTGCTGGAAGAAGAAGCTGCCAGTCTCACATGGAAGGCAGGACTGGAATTCGTATGGGAAGGCTCTCATTAAATTACCAAAGTCTGACGCTTCTTTAGAGGATCCACTTTCTCCAGTCTCAAACAGGGGAAAAAAAGATCAGAAAACTCTCATCTTAGTCGGAGGACTTCTTTTGGGTAGCTCTGTAGTTCTTAACTTTATATTTCTTGCAGCGATTTCTTTGGTTTTCTTTTATGGATACAAGAAAAGACATAATCTTACTAGTAGTGCATCAAGTATTATGGAAGCAAATCTACGCTTGTTTACTTATAAAGATCTAGAAGAAGCCACAGATGGATTTAGGGAAGAACTTGGAAGAGGTGCTTTTGGCACTGTATACAAAGGAATCATATCATCCATCAGTTCAACAAACTACGTCGCAATCAAGAAGTTGGACAAAATGGCGCAAGAAGGTGAGAAGGAATTCAAAGCAGAGGTGAGAGCAATAGCCAGGACTCACCACAAAAACTTGGTCAGATTGCTTGGGTTCTGTGATGAAGGGCCAAACAAACTTTTGGTCTATGAGTTTATGAGCAATGGAACATTGGCCAGCTTTCTCTTTGGGATTTCAAGGCCCGATTGGAACAAACGAATTCAAATTGCTTTCGGGATTGCAAGAGGACTCATGTACCTGCATGAGGAGTGTAGCACGCAGATCGATCCACTGCGATATCAAGCCCCACAACATACTTCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

656

Amino Acids

72.79

Weight (kDa)

6.58

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 80 - 169 1.2e-15 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 523 - 637 1e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 525 - 638 2.4e-15 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1539
AarI CACCTGC 1 cut(s) 1238
AatII GACGTC 1 cut(s) 860
Acc36I ACCTGC 2 cut(s) 1238, 1908
AccB1I GGYRCC 1 cut(s) 1226
AccB7I CCANNNNNTGG 3 cut(s) 246, 490, 845
AccI GTMKAC 1 cut(s) 1605
AccII CGCG 1 cut(s) 317
AciI CCGC 4 cut(s) 111, 300, 462, 1002
AclWI GGATC 6 cut(s) 289, 452, 465, 1325, 1338, 1922
AcoI YGGCCR 2 cut(s) 307, 1815
AcsI RAATTY 4 cut(s) 427, 1276, 1691, 1860
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 2 cut(s) 264, 857
AdeI CACNNNGTG 1 cut(s) 62
AfaI GTAC 2 cut(s) 1091, 1898
AfiI CCNNNNNNNGG 4 cut(s) 246, 490, 845, 1906
AgsI TTSAA 9 cut(s) 53, 454, 554, 1014, 1074, 1635, 1696, 1839, 1865
AhlI ACTAGT 1 cut(s) 1490
AjnI CCWGG 3 cut(s) 860, 991, 1718
AleI CACNNNNGTG 1 cut(s) 183
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 3 cut(s) 255, 1258, 1351
AlwI GGATC 6 cut(s) 289, 452, 465, 1325, 1338, 1922
AlwNI CAGNNNCTG 1 cut(s) 685
Ama87I CYCGRG 1 cut(s) 538
AoxI GGCC 6 cut(s) 25, 307, 325, 1769, 1815, 1841
ApeKI GCWGC 8 cut(s) 65, 68, 80, 386, 653, 682, 1246, 1439
ApoI RAATTY 4 cut(s) 427, 1276, 1691, 1860
ArsI GACNNNNNNTTYG 4 cut(s) 1725, 1757, 1770, 1802
Asp700I GAANNNNTTC 2 cut(s) 987, 1691
AspLEI GCGC 2 cut(s) 319, 1675
AspS9I GGNCC 4 cut(s) 326, 926, 1769, 1842
AsuC2I CCSGG 2 cut(s) 154, 884
AvaI CYCGRG 1 cut(s) 538
AvaII GGWCC 1 cut(s) 926
BaeI ACNNNNGTAYC 2 cut(s) 1880, 1913
BalI TGGCCA 2 cut(s) 309, 1817
BamHI GGATCC 2 cut(s) 457, 1330
BanI GGYRCC 1 cut(s) 1226
BanII GRGCYC 1 cut(s) 297
BarI GAAGNNNNNNTAC 2 cut(s) 1506, 1538
BbsI GAAGAC 3 cut(s) 126, 976, 980
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 8 cut(s) 67, 77, 80, 373, 640, 669, 1233, 1451
BccI CCATC 5 cut(s) 299, 566, 797, 1556, 1634
BcgI CGANNNNNNTGC 2 cut(s) 1906, 1940
BciT130I CCWGG 3 cut(s) 862, 993, 1720
BciVI GTATCC 2 cut(s) 929, 1460
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 2 cut(s) 154, 884
BcoDI GTCTC 3 cut(s) 255, 1258, 1351
BcuI ACTAGT 1 cut(s) 1490
BfaI CTAG 6 cut(s) 516, 581, 1079, 1491, 1547, 1962
BfmI CTRYAG 1 cut(s) 1413
BfuAI ACCTGC 2 cut(s) 1238, 1908
BfuI GTATCC 2 cut(s) 929, 1460
BglII AGATCT 2 cut(s) 1178, 1543
BisI GCNGC 8 cut(s) 66, 69, 81, 387, 654, 683, 1247, 1440
BlsI GCNGC 8 cut(s) 67, 70, 82, 388, 655, 684, 1248, 1441
Bme1390I CCNGG 5 cut(s) 154, 862, 884, 993, 1720
Bme18I GGWCC 1 cut(s) 926
BmeT110I CYCGRG 1 cut(s) 538
BmgT120I GGNCC 4 cut(s) 326, 926, 1769, 1842
BmiI GGNNCC 3 cut(s) 459, 1228, 1332
BmrFI CCNGG 5 cut(s) 154, 862, 884, 993, 1720
BmsI GCATC 4 cut(s) 122, 640, 949, 1508
BpiI GAAGAC 3 cut(s) 126, 976, 980
BpmI CTGGAG 2 cut(s) 512, 1326
BpuMI CCSGG 2 cut(s) 154, 884
Bsa29I ATCGAT 1 cut(s) 1926
BsaHI GRCGYC 2 cut(s) 264, 857
BsaWI WCCGGW 1 cut(s) 592
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 490, 845, 1906
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 5 cut(s) 798, 960, 1250, 1277, 1343
Bse3DI GCAATG 1 cut(s) 1810
BseBI CCWGG 3 cut(s) 862, 993, 1720
BseCI ATCGAT 1 cut(s) 1926
BseGI GGATG 2 cut(s) 964, 1622
BseLI CCNNNNNNNGG 4 cut(s) 246, 490, 845, 1906
BseMI GCAATG 1 cut(s) 1810
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 5 cut(s) 798, 960, 1250, 1277, 1343
BseRI GAGGAG 1 cut(s) 1922
BseXI GCAGC 8 cut(s) 67, 77, 80, 373, 640, 669, 1233, 1451
BseYI CCCAGC 1 cut(s) 268
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 6 cut(s) 27, 309, 327, 1771, 1817, 1843
BshNI GGYRCC 1 cut(s) 1226
BshVI ATCGAT 1 cut(s) 1926
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 538
BsiSI CCGG 4 cut(s) 153, 324, 593, 884
BslFI GGGAC 2 cut(s) 88, 169
BslI CCNNNNNNNGG 4 cut(s) 246, 490, 845, 1906
BsmAI GTCTC 3 cut(s) 255, 1258, 1351
BsmBI CGTCTC 1 cut(s) 255
BsmFI GGGAC 2 cut(s) 88, 169
BsnI GGCC 6 cut(s) 27, 309, 327, 1771, 1817, 1843
BsoBI CYCGRG 1 cut(s) 538
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
BspACI CCGC 4 cut(s) 111, 300, 462, 1002
BspANI GGCC 6 cut(s) 27, 309, 327, 1771, 1817, 1843
BspCNI CTCAG 1 cut(s) 346
BspDI ATCGAT 1 cut(s) 1926
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 3 cut(s) 459, 1228, 1332
BspMI ACCTGC 2 cut(s) 1238, 1908
BspPI GGATC 6 cut(s) 289, 452, 465, 1325, 1338, 1922
BspT107I GGYRCC 1 cut(s) 1226
BsrDI GCAATG 1 cut(s) 1810
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 5 cut(s) 798, 960, 1250, 1277, 1343
BssAI RCCGGY 1 cut(s) 323
BssMI GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
BssNAI GTATAC 1 cut(s) 1606
BssNI GRCGYC 2 cut(s) 264, 857
Bst1107I GTATAC 1 cut(s) 1606
Bst2UI CCWGG 3 cut(s) 862, 993, 1720
Bst4CI ACNGT 3 cut(s) 757, 930, 1603
Bst6I CTCTTC 1 cut(s) 1579
BstACI GRCGYC 2 cut(s) 264, 857
BstC8I GCNNGC 6 cut(s) 207, 417, 421, 529, 1819, 1919
BstDEI CTNAG 3 cut(s) 354, 365, 1384
BstENI CCTNNNNNAGG 1 cut(s) 1904
BstF5I GGATG 2 cut(s) 964, 1622
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 2 cut(s) 319, 1675
BstKTI GATC 9 cut(s) 190, 284, 460, 1181, 1333, 1369, 1546, 1926, 1930
BstMAI GTCTC 3 cut(s) 255, 1258, 1351
BstMBI GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
BstMWI GCNNNNNNNGC 6 cut(s) 77, 333, 386, 653, 1065, 1150
BstNI CCWGG 3 cut(s) 862, 993, 1720
BstSCI CCNGG 5 cut(s) 152, 860, 882, 991, 1718
BstSFI CTRYAG 1 cut(s) 1413
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 8 cut(s) 67, 77, 80, 373, 640, 669, 1233, 1451
BstV2I GAAGAC 3 cut(s) 126, 976, 980
BstX2I RGATCY 4 cut(s) 457, 1178, 1330, 1543
BstYI RGATCY 4 cut(s) 457, 1178, 1330, 1543
BstZ17I GTATAC 1 cut(s) 1606
Bsu15I ATCGAT 1 cut(s) 1926
BsuI GTATCC 2 cut(s) 929, 1460
BsuRI GGCC 6 cut(s) 27, 309, 327, 1771, 1817, 1843
BsuTUI ATCGAT 1 cut(s) 1926
BtsCI GGATG 2 cut(s) 964, 1622
BtsI GCAGTG 2 cut(s) 642, 1931
BtsIMutI CAGTG 5 cut(s) 642, 762, 791, 1599, 1931
BveI ACCTGC 2 cut(s) 1238, 1908
Cac8I GCNNGC 6 cut(s) 207, 417, 421, 529, 1819, 1919
CaiI CAGNNNCTG 1 cut(s) 685
CfoI GCGC 2 cut(s) 319, 1675
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 4 cut(s) 326, 926, 1769, 1842
ClaI ATCGAT 1 cut(s) 1926
CseI GACGC 2 cut(s) 272, 1325
Csp6I GTAC 2 cut(s) 1090, 1897
CviAII CATG 6 cut(s) 138, 391, 1100, 1259, 1894, 1904
CviQI GTAC 2 cut(s) 1090, 1897
DdeI CTNAG 3 cut(s) 354, 365, 1384
DpnI GATC 9 cut(s) 189, 283, 459, 1180, 1332, 1368, 1545, 1925, 1929
DpnII GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
DraIII CACNNNGTG 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 307, 1815
Eam1104I CTCTTC 1 cut(s) 1579
EarI CTCTTC 1 cut(s) 1579
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 1940
Eco47I GGWCC 1 cut(s) 926
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 538
EcoICRI GAGCTC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 1904
EcoRI GAATTC 4 cut(s) 427, 1276, 1691, 1860
EcoRII CCWGG 3 cut(s) 860, 991, 1718
EcoRV GATATC 1 cut(s) 1940
EcoT22I ATGCAT 1 cut(s) 1101
EcoT38I GRGCYC 1 cut(s) 297
Esp3I CGTCTC 1 cut(s) 255
FaeI CATG 6 cut(s) 141, 394, 1103, 1262, 1897, 1907
FalI AAGNNNNNCTT 2 cut(s) 1576, 1608
FaqI GGGAC 2 cut(s) 88, 169
FatI CATG 6 cut(s) 137, 390, 1099, 1258, 1893, 1903
FauI CCCGC 1 cut(s) 469
FauNDI CATATG 1 cut(s) 212
FbaI TGATCA 1 cut(s) 187
FblI GTMKAC 1 cut(s) 1605
Fnu4HI GCNGC 8 cut(s) 66, 69, 81, 387, 654, 683, 1247, 1440
FokI GGATG 2 cut(s) 971, 1609
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 8 cut(s) 66, 69, 81, 387, 654, 683, 1247, 1440
FspBI CTAG 6 cut(s) 516, 581, 1079, 1491, 1547, 1962
GlaI GCGC 2 cut(s) 318, 1674
GluI GCNGC 8 cut(s) 66, 69, 81, 387, 654, 683, 1247, 1440
GsaI CCCAGC 1 cut(s) 272
GsuI CTGGAG 2 cut(s) 512, 1326
HaeIII GGCC 6 cut(s) 27, 309, 327, 1771, 1817, 1843
HapII CCGG 4 cut(s) 153, 324, 593, 884
HgaI GACGC 2 cut(s) 272, 1325
HhaI GCGC 2 cut(s) 319, 1675
Hin1I GRCGYC 2 cut(s) 264, 857
Hin1II CATG 6 cut(s) 141, 394, 1103, 1262, 1897, 1907
Hin6I GCGC 2 cut(s) 317, 1673
HinP1I GCGC 2 cut(s) 317, 1673
HincII GTYRAC 1 cut(s) 1107
HindII GTYRAC 1 cut(s) 1107
HinfI GANTC 6 cut(s) 356, 695, 1010, 1614, 1723, 1890
HpaII CCGG 4 cut(s) 153, 324, 593, 884
Hpy166II GTNNAC 5 cut(s) 926, 1037, 1107, 1533, 1606
Hpy188I TCNGA 6 cut(s) 118, 433, 1315, 1371, 1391, 1745
Hpy188III TCNNGA 6 cut(s) 191, 346, 1547, 1654, 1876, 1962
Hpy8I GTNNAC 5 cut(s) 926, 1037, 1107, 1533, 1606
Hpy99I CGWCG 1 cut(s) 1649
HpyCH4III ACNGT 3 cut(s) 757, 930, 1603
HpyCH4IV ACGT 4 cut(s) 180, 857, 1092, 1644
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 333, 386, 653, 1065, 1150
HpyF3I CTNAG 3 cut(s) 354, 365, 1384
HpySE526I ACGT 4 cut(s) 180, 857, 1092, 1644
Hsp92I GRCGYC 2 cut(s) 264, 857
Hsp92II CATG 6 cut(s) 141, 394, 1103, 1262, 1897, 1907
HspAI GCGC 2 cut(s) 317, 1673
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
Lsp1109I GCAGC 8 cut(s) 67, 77, 80, 373, 640, 669, 1233, 1451
LweI GCATC 4 cut(s) 122, 640, 949, 1508
MaeI CTAG 6 cut(s) 516, 581, 1079, 1491, 1547, 1962
MaeII ACGT 4 cut(s) 180, 857, 1092, 1644
MaeIII GTNAC 4 cut(s) 144, 701, 715, 759
MalI GATC 9 cut(s) 189, 283, 459, 1180, 1332, 1368, 1545, 1925, 1929
MboI GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
MflI RGATCY 4 cut(s) 457, 1178, 1330, 1543
MhlI GDGCHC 1 cut(s) 297
MlsI TGGCCA 2 cut(s) 309, 1817
MluNI TGGCCA 2 cut(s) 309, 1817
MlyI GAGTC 4 cut(s) 365, 704, 1717, 1884
MmeI TCCRAC 2 cut(s) 1369, 1641
Mox20I TGGCCA 2 cut(s) 309, 1817
Mph1103I ATGCAT 1 cut(s) 1101
MroXI GAANNNNTTC 2 cut(s) 987, 1691
MscI TGGCCA 2 cut(s) 309, 1817
MseI TTAA 3 cut(s) 690, 1299, 1422
MslI CAYNNNNRTG 1 cut(s) 183
Msp20I TGGCCA 2 cut(s) 309, 1817
MspA1I CMGCKG 2 cut(s) 302, 682
MspI CCGG 4 cut(s) 153, 324, 593, 884
MspR9I CCNGG 5 cut(s) 154, 862, 884, 993, 1720
MvaI CCWGG 3 cut(s) 862, 993, 1720
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 6 cut(s) 77, 333, 386, 653, 1065, 1150
NciI CCSGG 2 cut(s) 154, 884
NdeI CATATG 1 cut(s) 212
NdeII GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
NlaIII CATG 6 cut(s) 141, 394, 1103, 1262, 1897, 1907
NlaIV GGNNCC 3 cut(s) 459, 1228, 1332
NmuCI GTSAC 3 cut(s) 144, 715, 759
NsiI ATGCAT 1 cut(s) 1101
OliI CACNNNNGTG 1 cut(s) 183
PaeR7I CTCGAG 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 1238
PdmI GAANNNNTTC 2 cut(s) 987, 1691
PfeI GAWTC 2 cut(s) 1010, 1614
PflMI CCANNNNNTGG 3 cut(s) 246, 490, 845
PfoI TCCNGGA 2 cut(s) 152, 882
PkrI GCNGC 8 cut(s) 67, 70, 82, 388, 655, 684, 1248, 1441
PleI GAGTC 4 cut(s) 364, 703, 1717, 1884
PpsI GAGTC 4 cut(s) 364, 703, 1717, 1884
PsiI TTATAA 1 cut(s) 1539
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 3 cut(s) 860, 991, 1718
PspFI CCCAGC 1 cut(s) 268
PspGI CCWGG 3 cut(s) 860, 991, 1718
PspN4I GGNNCC 3 cut(s) 459, 1228, 1332
PspPI GGNCC 4 cut(s) 326, 926, 1769, 1842
PspXI VCTCGAGB 1 cut(s) 538
PstNI CAGNNNCTG 1 cut(s) 685
PsuI RGATCY 4 cut(s) 457, 1178, 1330, 1543
PvuII CAGCTG 1 cut(s) 682
RsaI GTAC 2 cut(s) 1091, 1898
RsaNI GTAC 2 cut(s) 1090, 1897
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 690, 1299, 1422
SatI GCNGC 8 cut(s) 66, 69, 81, 387, 654, 683, 1247, 1440
Sau3AI GATC 9 cut(s) 187, 281, 457, 1178, 1330, 1366, 1543, 1923, 1927
Sau96I GGNCC 4 cut(s) 326, 926, 1769, 1842
SchI GAGTC 4 cut(s) 365, 704, 1717, 1884
ScrFI CCNGG 5 cut(s) 154, 862, 884, 993, 1720
SduI GDGCHC 1 cut(s) 297
SfaNI GCATC 4 cut(s) 122, 640, 949, 1508
SfcI CTRYAG 1 cut(s) 1413
Sfr274I CTCGAG 1 cut(s) 538
SinI GGWCC 1 cut(s) 926
SlaI CTCGAG 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
SpeI ACTAGT 1 cut(s) 1490
SsiI CCGC 4 cut(s) 111, 300, 462, 1002
SspI AATATT 2 cut(s) 88, 712
SspMI CTAG 6 cut(s) 516, 581, 1079, 1491, 1547, 1962
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 5 cut(s) 152, 860, 882, 991, 1718
TaaI ACNGT 3 cut(s) 757, 930, 1603
TaiI ACGT 4 cut(s) 183, 860, 1095, 1647
TaqI TCGA 3 cut(s) 284, 539, 1926
TfiI GAWTC 2 cut(s) 1010, 1614
Tru1I TTAA 3 cut(s) 690, 1299, 1422
Tru9I TTAA 3 cut(s) 690, 1299, 1422
TscAI CASTG 5 cut(s) 649, 762, 798, 1606, 1938
TseFI GTSAC 3 cut(s) 144, 715, 759
TseI GCWGC 8 cut(s) 65, 68, 80, 386, 653, 682, 1246, 1439
Tsp45I GTSAC 3 cut(s) 144, 715, 759
TspDTI ATGAA 4 cut(s) 680, 1172, 1176, 1779
TspGWI ACGGA 2 cut(s) 349, 623
TspRI CASTG 5 cut(s) 649, 762, 798, 1606, 1938
Van91I CCANNNNNTGG 3 cut(s) 246, 490, 845
VpaK11BI GGWCC 1 cut(s) 926
XagI CCTNNNNNAGG 1 cut(s) 1904
XapI RAATTY 4 cut(s) 427, 1276, 1691, 1860
XbaI TCTAGA 2 cut(s) 1546, 1961
XhoI CTCGAG 1 cut(s) 538
XmiI GTMKAC 1 cut(s) 1605
XmnI GAANNNNTTC 2 cut(s) 987, 1691
XspI CTAG 6 cut(s) 516, 581, 1079, 1491, 1547, 1962
ZraI GACGTC 1 cut(s) 858
Zsp2I ATGCAT 1 cut(s) 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.