AT2G35120

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005002
Physical Location & Seq
Reverse (-)
14805639 .. 14807406
1768 bp
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UTR
Exon/CDS
Intron
AT2G35120.1

Sequence Viewer

Length: 471 bp
ATGGCTTGTAGACTCTTTTGGGCTTCTAGGGTTGCTTCTCATCTAAGGATCTCCGTTGCTCAACGAGGGTTTTCTTCCGTGGTTTTGAAGGATTTGAAATATGCTGATTCACATGAATGGGTGAAGATTGACGGGAATAAAGCAACCTTTGGTATAACGGATCACGCGCAGGACCATTTAGGAGATGTGGTCTATGTGGAGTTACCTGATGTAGGACATTCGGTGTCACAAGGGAAGAGTTTTGGAGCAGTTGAAAGTGTGAAAGCAACTAGCGATATCAATTCTCCAGTCTCGGGTAAGGTGGTTGAAGTCAATGAGGAGCTGACCGAGTCCCCTGGATTGGTGAACTCTAGCCCGTATGAACAAGGATGGATCATAAAAGTTGAGCTGAGTGATGCAGGCGAGGCAGAGAAGCTGATGGATTCAGACAAGTACTCTAAGTTCTGCGAAGAAGAAGACGCCAAGCACTGA

Protein Analysis

156

Amino Acids

17.1

Weight (kDa)

4.97

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 32 - 151 5.8e-49 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 10
AccII CGCG 1 cut(s) 167
AclWI GGATC 3 cut(s) 56, 168, 380
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 3 cut(s) 212, 293, 340
AgsI TTSAA 4 cut(s) 88, 97, 254, 308
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 3 cut(s) 322, 388, 415
AluI AGCT 3 cut(s) 322, 388, 415
Alw26I GTCTC 1 cut(s) 295
AlwI GGATC 3 cut(s) 56, 168, 380
Ama87I CYCGRG 1 cut(s) 292
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 133, 355
AvaI CYCGRG 1 cut(s) 292
AvaII GGWCC 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 462
BccI CCATC 2 cut(s) 363, 412
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 295
BfaI CTAG 3 cut(s) 27, 270, 351
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 172
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 1 cut(s) 385
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 2 cut(s) 78, 334
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 293, 340
Bse1I ACTGG 1 cut(s) 287
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 78, 334
BseGI GGATG 1 cut(s) 374
BseLI CCNNNNNNNGG 3 cut(s) 212, 293, 340
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 167
BsiHKCI CYCGRG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 3 cut(s) 212, 293, 340
BsmAI GTCTC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 316
BsoBI CYCGRG 1 cut(s) 292
Bsp143I GATC 3 cut(s) 48, 160, 372
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 1 cut(s) 167
BspPI GGATC 3 cut(s) 56, 168, 380
BsrI ACTGG 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 78, 334
BssMI GATC 3 cut(s) 48, 160, 372
BssNI GRCGYC 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 336
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 459
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 3 cut(s) 44, 389, 438
BstDSI CCRYGG 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 374
BstFNI CGCG 1 cut(s) 167
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 3 cut(s) 51, 163, 375
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 3 cut(s) 48, 160, 372
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstUI CGCG 1 cut(s) 167
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 1 cut(s) 374
BtsIMutI CAGTG 1 cut(s) 466
Cac8I GCNNGC 1 cut(s) 400
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 433
CviAII CATG 1 cut(s) 113
CviJI RGCY 6 cut(s) 5, 23, 322, 354, 388, 415
CviKI_1 RGCY 6 cut(s) 5, 23, 322, 354, 388, 415
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 3 cut(s) 44, 389, 438
DpnI GATC 3 cut(s) 50, 162, 374
DpnII GATC 3 cut(s) 48, 160, 372
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 292
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 1 cut(s) 277
FaeI CATG 1 cut(s) 116
FaiI YATR 6 cut(s) 102, 114, 155, 195, 360, 377
FaqI GGGAC 1 cut(s) 316
FatI CATG 1 cut(s) 112
FblI GTMKAC 1 cut(s) 10
FokI GGATG 1 cut(s) 381
FspBI CTAG 3 cut(s) 27, 270, 351
GlaI GCGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 270
HhaI GCGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 1 cut(s) 116
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 4 cut(s) 12, 107, 329, 422
HphI GGTGA 2 cut(s) 133, 355
Hpy166II GTNNAC 2 cut(s) 11, 346
Hpy188I TCNGA 1 cut(s) 427
Hpy8I GTNNAC 2 cut(s) 11, 346
HpyAV CCTTC 1 cut(s) 82
HpyCH4V TGCA 1 cut(s) 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 3 cut(s) 44, 389, 438
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 1 cut(s) 116
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 3 cut(s) 48, 160, 372
LmnI GCTCC 2 cut(s) 245, 319
LpnPI CCDG 6 cut(s) 155, 219, 300, 321, 348, 384
LweI GCATC 1 cut(s) 385
MaeI CTAG 3 cut(s) 27, 270, 351
MaeIII GTNAC 2 cut(s) 201, 225
MalI GATC 3 cut(s) 50, 162, 374
MboI GATC 3 cut(s) 48, 160, 372
MboII GAAGA 6 cut(s) 66, 136, 247, 461, 464, 467
MflI RGATCY 1 cut(s) 48
MluCI AATT 1 cut(s) 280
MlyI GAGTC 2 cut(s) 6, 338
MnlI CCTC 3 cut(s) 59, 310, 397
MslI CAYNNNNRTG 1 cut(s) 115
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeII GATC 3 cut(s) 48, 160, 372
NlaIII CATG 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 225
PfeI GAWTC 2 cut(s) 107, 422
PflFI GACNNNGTC 1 cut(s) 328
PleI GAGTC 2 cut(s) 6, 337
PpsI GAGTC 2 cut(s) 6, 337
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspPI GGNCC 1 cut(s) 172
PsrI GAACNNNNNNTAC 2 cut(s) 425, 457
PsuI RGATCY 1 cut(s) 48
PsyI GACNNNGTC 1 cut(s) 328
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 115
Sau3AI GATC 3 cut(s) 48, 160, 372
Sau96I GGNCC 1 cut(s) 172
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 2 cut(s) 6, 338
ScrFI CCNGG 1 cut(s) 336
SetI ASST 6 cut(s) 149, 208, 303, 324, 390, 417
SfaNI GCATC 1 cut(s) 385
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 115
Sse9I AATT 1 cut(s) 280
SspMI CTAG 3 cut(s) 27, 270, 351
StyD4I CCNGG 1 cut(s) 334
TaqII GACCGA 1 cut(s) 341
TasI AATT 1 cut(s) 280
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 2 cut(s) 107, 422
TseFI GTSAC 1 cut(s) 225
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 2 cut(s) 129, 375
TspGWI ACGGA 3 cut(s) 43, 67, 173
Tth111I GACNNNGTC 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 172
XagI CCTNNNNNAGG 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 10
XspI CTAG 3 cut(s) 27, 270, 351
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.