FvH4_6g39570

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005002
Physical Location & Seq
Reverse (-)
31245259 .. 31246502
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39570.t1

Sequence Viewer

Length: 501 bp
ATGGCATTGAGTATGTTTTGCGCAACCAGGGCTTCTCTGTATCTGATGAACATGAACACCTCAGGTCCTTCCTCTAGAGGCTTTTCTTCTGTTGTAAAGAGTTTGAAGTATGCCAAGTCGCATGAGTGGGCGAGAGTTGATGGGAAGTTGGCTACAATAGGGTTGAGTGACTATGCTCAAAAGCACTTGGGTGGTATTATCCATGTTGAACTGCCTGATACCGGTGAAGATGTGGCACAAGGTGTTAGATGTGGCACTGTTGAGTCTGTCCAAGCCAGCAAGGGCCTTCACTCTTCACTCTCTGGCAATATAGTTGAAGTAAATGAAAAGCTTCGTACGACTGCTGGTTTGGTAAATGCAAGTCCATATGAAGATGGATGGATCATGAAGGTGGAAATGAGCAACAAAGAGGAGCTGAATAAGCTAATGGACTATGATGAGTACGTGAGCTATGTGGAGAACAAGTTCCCAAACATTGATGAACCAGTTGCGAACACTTAG

Protein Analysis

167

Amino Acids

18.17

Weight (kDa)

5.36

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 35 - 154 7.2e-39 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 22
AclWI GGATC 1 cut(s) 389
AdeI CACNNNGTG 1 cut(s) 242
AfaI GTAC 2 cut(s) 337, 443
AfiI CCNNNNNNNGG 1 cut(s) 221
AgeI ACCGGT 1 cut(s) 221
AgsI TTSAA 3 cut(s) 106, 209, 317
AjnI CCWGG 1 cut(s) 26
AleI CACNNNNGTG 1 cut(s) 189
AloI GAACNNNNNNTCC 2 cut(s) 449, 481
AluBI AGCT 4 cut(s) 331, 415, 424, 450
AluI AGCT 4 cut(s) 331, 415, 424, 450
AlwI GGATC 1 cut(s) 389
AoxI GGCC 1 cut(s) 283
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
AsiGI ACCGGT 1 cut(s) 221
Asp700I GAANNNNTTC 2 cut(s) 330, 464
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 2 cut(s) 65, 283
AsuHPI GGTGA 1 cut(s) 236
AvaII GGWCC 1 cut(s) 65
AxyI CCTNAGG 1 cut(s) 61
BccI CCATC 3 cut(s) 134, 368, 372
BciT130I CCWGG 1 cut(s) 28
BfaI CTAG 1 cut(s) 75
Bme1390I CCNGG 1 cut(s) 28
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 65, 283
BmrFI CCNGG 1 cut(s) 28
BsaAI YACGTR 1 cut(s) 445
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 221
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 485
Bse21I CCTNAGG 1 cut(s) 61
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 383
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 1 cut(s) 425
BshFI GGCC 1 cut(s) 285
BshTI ACCGGT 1 cut(s) 221
BsiSI CCGG 1 cut(s) 222
BsiWI CGTACG 1 cut(s) 335
BslI CCNNNNNNNGG 1 cut(s) 221
BsnI GGCC 1 cut(s) 285
Bsp143I GATC 1 cut(s) 381
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 384
BspPI GGATC 1 cut(s) 389
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 1 cut(s) 485
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 298
BstBAI YACGTR 1 cut(s) 445
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 2 cut(s) 61, 498
BstF5I GGATG 1 cut(s) 383
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 1 cut(s) 384
BstMBI GATC 1 cut(s) 381
BstMWI GCNNNNNNNGC 2 cut(s) 29, 421
BstNI CCWGG 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 26
Bsu36I CCTNAGG 1 cut(s) 61
BsuRI GGCC 1 cut(s) 285
BtsCI GGATG 1 cut(s) 383
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 277
CciI TCATGA 1 cut(s) 384
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 65, 283
Csp6I GTAC 2 cut(s) 336, 442
CspAI ACCGGT 1 cut(s) 221
CviAII CATG 4 cut(s) 52, 122, 203, 385
CviJI RGCY 9 cut(s) 32, 81, 152, 275, 285, 331, 415, 424, 450
CviKI_1 RGCY 9 cut(s) 32, 81, 152, 275, 285, 331, 415, 424, 450
CviQI GTAC 2 cut(s) 336, 442
DdeI CTNAG 2 cut(s) 61, 498
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DraIII CACNNNGTG 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 61
EcoO109I RGGNCCY 2 cut(s) 65, 283
EcoRII CCWGG 1 cut(s) 26
FaeI CATG 4 cut(s) 55, 125, 206, 388
FatI CATG 4 cut(s) 51, 121, 202, 384
FauNDI CATATG 1 cut(s) 367
FokI GGATG 1 cut(s) 390
FspBI CTAG 1 cut(s) 75
FspI TGCGCA 1 cut(s) 22
GlaI GCGC 1 cut(s) 22
HaeIII GGCC 1 cut(s) 285
HapII CCGG 1 cut(s) 222
HhaI GCGC 1 cut(s) 23
Hin1II CATG 4 cut(s) 55, 125, 206, 388
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 1 cut(s) 263
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 236
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 2 cut(s) 75, 385
HpyAV CCTTC 3 cut(s) 78, 296, 382
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 421
HpyF3I CTNAG 2 cut(s) 61, 498
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 4 cut(s) 55, 125, 206, 388
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 381
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 8 cut(s) 13, 40, 48, 228, 235, 288, 289, 330
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MboII GAAGA 4 cut(s) 78, 239, 285, 383
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 4 cut(s) 70, 71, 82, 403
MroXI GAANNNNTTC 2 cut(s) 330, 464
MslI CAYNNNNRTG 2 cut(s) 189, 389
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 28
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 2 cut(s) 29, 421
NdeI CATATG 1 cut(s) 367
NdeII GATC 1 cut(s) 381
NlaIII CATG 4 cut(s) 55, 125, 206, 388
NmuCI GTSAC 1 cut(s) 167
NsbI TGCGCA 1 cut(s) 22
OliI CACNNNNGTG 1 cut(s) 189
PagI TCATGA 1 cut(s) 384
PdmI GAANNNNTTC 2 cut(s) 330, 464
Pfl23II CGTACG 1 cut(s) 335
PinAI ACCGGT 1 cut(s) 221
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 445
PpuMI RGGWCCY 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspLI CGTACG 1 cut(s) 335
PspPI GGNCC 2 cut(s) 65, 283
PspPPI RGGWCCY 1 cut(s) 65
RsaI GTAC 2 cut(s) 337, 443
RsaNI GTAC 2 cut(s) 336, 442
RseI CAYNNNNRTG 2 cut(s) 189, 389
Sau3AI GATC 1 cut(s) 381
Sau96I GGNCC 2 cut(s) 65, 283
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 28
SetI ASST 9 cut(s) 62, 67, 244, 333, 393, 417, 426, 447, 452
SinI GGWCC 1 cut(s) 65
SmiMI CAYNNNNRTG 2 cut(s) 189, 389
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 1 cut(s) 447
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 6 cut(s) 62, 68, 339, 384, 401, 495
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 65
XbaI TCTAGA 1 cut(s) 74
XmnI GAANNNNTTC 2 cut(s) 330, 464
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.