MD09G1138700.v1.1

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005002
Physical Location & Seq
Forward (+)
10782173 .. 10782786
614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1138700.v1.1.491

Sequence Viewer

Length: 312 bp
ATGCAAAGTATCACGAGTGGGCGAAAGTTGACGAAGGGAAGTTTGGCTACAGTAGGAATATCAGACCATGCTCAGGAACATTTAGGTGACATTATTTACGTAGAGCTGCCTGATACTGTTGTTGCAGTTACACAAGCCAGCACTGTTGGAAGTGTTGAGAGTGTGAAAGCCTCCACCGACATCAAGAGTCCTGTCTCGGGCAACATAATTGAAGTGAACAAAGAGCTTCTTAGCTCTCCAGGTTTGGTAAATGGAAGCCCTTATGAAAAAGGATGGATCACCAAGGTGGAGATGAGTACTCTGAGTGTGTGA

Protein Analysis

104

Amino Acids

10.78

Weight (kDa)

5.21

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 13 - 101 3.9e-32 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 284
AfaI GTAC 1 cut(s) 298
AfiI CCNNNNNNNGG 2 cut(s) 73, 197
AgsI TTSAA 1 cut(s) 212
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AleI CACNNNNGTG 1 cut(s) 284
AluBI AGCT 3 cut(s) 106, 226, 234
AluI AGCT 3 cut(s) 106, 226, 234
Alw26I GTCTC 1 cut(s) 199
AlwI GGATC 1 cut(s) 284
Ama87I CYCGRG 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 98, 271
AvaI CYCGRG 1 cut(s) 196
BaeI ACNNNNGTAYC 2 cut(s) 105, 138
BarI GAAGNNNNNNTAC 2 cut(s) 31, 63
BauI CACGAG 1 cut(s) 13
BbvI GCAGC 1 cut(s) 93
BccI CCATC 1 cut(s) 267
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 199
BfmI CTRYAG 1 cut(s) 48
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 240
BmeT110I CYCGRG 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 222
Bpu10I CCTNAGC 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 197
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 278
BseLI CCNNNNNNNGG 2 cut(s) 73, 197
BseMII CTCAG 2 cut(s) 86, 293
BseXI GCAGC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 196
BslI CCNNNNNNNGG 2 cut(s) 73, 197
BsmAI GTCTC 1 cut(s) 199
BsoBI CYCGRG 1 cut(s) 196
Bsp143I GATC 1 cut(s) 276
BspCNI CTCAG 2 cut(s) 85, 294
BspPI GGATC 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 1 cut(s) 276
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 3 cut(s) 52, 118, 145
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 3 cut(s) 72, 230, 302
BstF5I GGATG 1 cut(s) 278
BstKTI GATC 1 cut(s) 279
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 1 cut(s) 276
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 1 cut(s) 48
BstSNI TACGTA 1 cut(s) 100
BstV1I GCAGC 1 cut(s) 93
BtsCI GGATG 1 cut(s) 278
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 297
CviAII CATG 1 cut(s) 68
CviJI RGCY 7 cut(s) 47, 106, 137, 170, 226, 234, 258
CviKI_1 RGCY 7 cut(s) 47, 106, 137, 170, 226, 234, 258
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 3 cut(s) 72, 230, 302
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
Eco105I TACGTA 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 1 cut(s) 71
FaiI YATR 3 cut(s) 69, 206, 264
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FatI CATG 1 cut(s) 67
Fnu4HI GCNGC 1 cut(s) 107
FokI GGATG 1 cut(s) 285
Fsp4HI GCNGC 1 cut(s) 107
GluI GCNGC 1 cut(s) 107
GsuI CTGGAG 1 cut(s) 222
Hin1II CATG 1 cut(s) 71
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 1 cut(s) 187
HphI GGTGA 2 cut(s) 98, 271
Hpy166II GTNNAC 2 cut(s) 30, 217
Hpy188I TCNGA 2 cut(s) 64, 303
Hpy188III TCNNGA 3 cut(s) 13, 74, 184
Hpy8I GTNNAC 2 cut(s) 30, 217
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 3 cut(s) 52, 118, 145
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 2 cut(s) 4, 125
HpyF3I CTNAG 3 cut(s) 72, 230, 302
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 1 cut(s) 71
Kzo9I GATC 1 cut(s) 276
LpnPI CCDG 6 cut(s) 59, 123, 151, 204, 225, 252
Lsp1109I GCAGC 1 cut(s) 93
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 86, 127
MalI GATC 1 cut(s) 278
MboI GATC 1 cut(s) 276
MluCI AATT 1 cut(s) 207
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 1 cut(s) 181
MslI CAYNNNNRTG 2 cut(s) 84, 284
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
NdeII GATC 1 cut(s) 276
NlaIII CATG 1 cut(s) 71
NmuCI GTSAC 1 cut(s) 86
OliI CACNNNNGTG 1 cut(s) 284
PkrI GCNGC 1 cut(s) 108
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
Ppu21I YACGTR 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
RseI CAYNNNNRTG 2 cut(s) 84, 284
SatI GCNGC 1 cut(s) 107
Sau3AI GATC 1 cut(s) 276
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 1 cut(s) 240
SetI ASST 7 cut(s) 88, 102, 108, 228, 236, 244, 288
SfcI CTRYAG 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 84, 284
SnaBI TACGTA 1 cut(s) 100
Sse9I AATT 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 238
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 3 cut(s) 52, 118, 145
TaiI ACGT 1 cut(s) 102
TasI AATT 1 cut(s) 207
TatI WGTACW 1 cut(s) 296
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 279
TspRI CASTG 1 cut(s) 148
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.