Rw2G041380

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005002
Physical Location & Seq
Reverse (-)
67399639 .. 67402573
2935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041380.1

Sequence Viewer

Length: 429 bp
ATGGTATTGAGTAGTATGTACATGAACACCTCAGTTCCTTCCTCTAGCGGCTTTTCTTCTGTTGTGAAGAGTTTAAAGTATGACAAGTCGCATGAGTGGGCGAGAGTTGATGGGATGTTGGCTACAATAGGGTTAAGTGATCCTGCTCAGAAGCACTTGGGTGGTATTATTATCCATGTTGAACTGCCTGATGTCGGTGAAGATGTAGCACAAGGCACCAGATGTGGTACTTCTGTCAAAGCCAGCAAGGGCCTCCACTCTCCTCTCTCTGGCAATGTAGTTGAAGTAAATCAAAAGTTTCGTACCTCTCCTGCTTTGATCGAAATGAGCAACAAAGAGGAGCTGAATAATCTGATGGACTATGATGAGTACGCGAGCTATGTGAAAACCAAGTTGCCAAACATTGATGAACCAGTTGAAAACACTTAG

Protein Analysis

142

Amino Acids

15.46

Weight (kDa)

5.29

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 25 - 110 2.9e-19 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 215
AccII CGCG 1 cut(s) 374
AciI CCGC 1 cut(s) 48
AclWI GGATC 1 cut(s) 134
AfaI GTAC 4 cut(s) 20, 229, 304, 371
AfiI CCNNNNNNNGG 2 cut(s) 194, 269
AgsI TTSAA 3 cut(s) 182, 284, 419
AleI CACNNNNGTG 1 cut(s) 159
AluBI AGCT 2 cut(s) 343, 378
AluI AGCT 2 cut(s) 343, 378
AlwI GGATC 1 cut(s) 134
AoxI GGCC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 209
BaeI ACNNNNGTAYC 2 cut(s) 219, 252
BanI GGYRCC 1 cut(s) 215
BccI CCATC 2 cut(s) 104, 349
BfaI CTAG 1 cut(s) 45
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 217
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 269
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 280
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 194, 269
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 45, 161
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 2 cut(s) 252, 353
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 1 cut(s) 252
BshNI GGYRCC 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 194, 269
BsnI GGCC 1 cut(s) 252
Bsp1407I TGTACA 1 cut(s) 18
Bsp143I GATC 2 cut(s) 139, 318
BspACI CCGC 1 cut(s) 48
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 2 cut(s) 44, 160
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 1 cut(s) 217
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 280
BsrGI TGTACA 1 cut(s) 18
BsrI ACTGG 1 cut(s) 413
BssMI GATC 2 cut(s) 139, 318
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 244, 376
BstDEI CTNAG 3 cut(s) 31, 147, 426
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 374
BstKTI GATC 2 cut(s) 142, 321
BstMBI GATC 2 cut(s) 139, 318
BstUI CGCG 1 cut(s) 374
BsuRI GGCC 1 cut(s) 252
BtsCI GGATG 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 244, 376
Cfr13I GGNCC 1 cut(s) 250
Csp6I GTAC 4 cut(s) 19, 228, 303, 370
CviAII CATG 3 cut(s) 22, 92, 176
CviJI RGCY 6 cut(s) 51, 122, 242, 252, 343, 378
CviKI_1 RGCY 6 cut(s) 51, 122, 242, 252, 343, 378
CviQI GTAC 4 cut(s) 19, 228, 303, 370
DdeI CTNAG 3 cut(s) 31, 147, 426
DpnI GATC 2 cut(s) 141, 320
DpnII GATC 2 cut(s) 139, 318
DraI TTTAAA 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 250
FaeI CATG 3 cut(s) 25, 95, 179
FaiI YATR 7 cut(s) 17, 23, 81, 93, 177, 363, 381
FatI CATG 3 cut(s) 21, 91, 175
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 1 cut(s) 127
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 1 cut(s) 45
GluI GCNGC 1 cut(s) 49
HaeIII GGCC 1 cut(s) 252
Hin1II CATG 3 cut(s) 25, 95, 179
HphI GGTGA 1 cut(s) 209
Hpy188I TCNGA 2 cut(s) 150, 354
HpyAV CCTTC 1 cut(s) 48
HpyF3I CTNAG 3 cut(s) 31, 147, 426
Hsp92II CATG 3 cut(s) 25, 95, 179
Kzo9I GATC 2 cut(s) 139, 318
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 6 cut(s) 156, 201, 232, 255, 256, 324
MaeI CTAG 1 cut(s) 45
MalI GATC 2 cut(s) 141, 320
MboI GATC 2 cut(s) 139, 318
MboII GAAGA 3 cut(s) 48, 79, 212
MnlI CCTC 6 cut(s) 40, 52, 263, 273, 316, 331
MseI TTAA 2 cut(s) 74, 134
MslI CAYNNNNRTG 1 cut(s) 159
MvnI CGCG 1 cut(s) 374
NdeII GATC 2 cut(s) 139, 318
NlaIII CATG 3 cut(s) 25, 95, 179
NlaIV GGNNCC 1 cut(s) 217
OliI CACNNNNGTG 1 cut(s) 159
PkrI GCNGC 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 250
RsaI GTAC 4 cut(s) 20, 229, 304, 371
RsaNI GTAC 4 cut(s) 19, 228, 303, 370
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 74, 134
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 2 cut(s) 139, 318
Sau96I GGNCC 1 cut(s) 250
SetI ASST 4 cut(s) 32, 308, 345, 380
SmiMI CAYNNNNRTG 1 cut(s) 159
SsiI CCGC 1 cut(s) 48
SspMI CTAG 1 cut(s) 45
TaqI TCGA 1 cut(s) 321
TatI WGTACW 1 cut(s) 18
TauI GCSGC 1 cut(s) 51
Tru1I TTAA 2 cut(s) 74, 134
Tru9I TTAA 2 cut(s) 74, 134
TspDTI ATGAA 2 cut(s) 38, 423
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.