Rh2CG489500

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0005002
Physical Location & Seq
Reverse (-)
65602517 .. 65604151
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG489500.1

Sequence Viewer

Length: 489 bp
ATGGTATTGAGTAGTATGTTTTGCGCAACGAGGGCTTCTCTGTATCTGATGAACATGAAAACCTCAGTTCCTTCCTCTAGAGGCTTTTCTTCTGTTGTGAAGAGTTTAAAGTATGACAAGTCGCATGAGTGGGCAAGAGTTGATGGGATGTTGGCTACAATAGGGTTAAGTGATCCTGCTCAGAAGCACTTGGGTGATGTAGCACAAGGCACCAGATGTGGTACTGTTGAGTCTGTCAAAGCCAGCAAGGGCCTCCACTCTCCTCTCTCTGGCAATGTAATTGAAGTAAATCAAAAGCTTCGTACCTCTCCTGCTTTGGTGAATGGGAGCCCGTATGAAGATGGATGGATCATGAAGGTAGAAATGAGCAACAAAGAGGAGCTGAATAATCTGATGGACTATGATGAGTACGCGAGCTATGTGAAAACCAAGTTGCCAAACATTGATGAACCAGTTGAAAACACTTGGAATATGTTGTATCAGAAGTAA

Protein Analysis

162

Amino Acids

18.02

Weight (kDa)

6.83

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 36 - 143 4.2e-30 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 209
AccII CGCG 1 cut(s) 413
AclWI GGATC 2 cut(s) 167, 356
AfaI GTAC 3 cut(s) 223, 304, 410
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 2 cut(s) 284, 458
AleI CACNNNNGTG 1 cut(s) 192
AluBI AGCT 3 cut(s) 298, 382, 417
AluI AGCT 3 cut(s) 298, 382, 417
AlwI GGATC 2 cut(s) 167, 356
AoxI GGCC 1 cut(s) 250
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 1 cut(s) 250
AsuHPI GGTGA 2 cut(s) 206, 331
BanI GGYRCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 332
BccI CCATC 4 cut(s) 137, 335, 339, 388
BfaI CTAG 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 2 cut(s) 211, 329
BplI GAGNNNNNCTC 2 cut(s) 22, 54
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 280
BseGI GGATG 2 cut(s) 153, 350
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 78, 194
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 2 cut(s) 252, 392
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 252
BshNI GGYRCC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 269
BsnI GGCC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 2 cut(s) 172, 348
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 2 cut(s) 77, 193
BspFNI CGCG 1 cut(s) 413
BspHI TCATGA 1 cut(s) 351
BspLI GGNNCC 2 cut(s) 211, 329
BspPI GGATC 2 cut(s) 167, 356
BspT107I GGYRCC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 280
BsrI ACTGG 1 cut(s) 452
BssMI GATC 2 cut(s) 172, 348
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 1 cut(s) 95
BstC8I GCNNGC 2 cut(s) 244, 415
BstDEI CTNAG 2 cut(s) 64, 180
BstF5I GGATG 2 cut(s) 153, 350
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 2 cut(s) 175, 351
BstMBI GATC 2 cut(s) 172, 348
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstUI CGCG 1 cut(s) 413
BsuRI GGCC 1 cut(s) 252
BtsCI GGATG 2 cut(s) 153, 350
Cac8I GCNNGC 2 cut(s) 244, 415
CciI TCATGA 1 cut(s) 351
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 250
Csp6I GTAC 3 cut(s) 222, 303, 409
CviAII CATG 3 cut(s) 55, 125, 352
CviJI RGCY 9 cut(s) 35, 84, 155, 242, 252, 298, 330, 382, 417
CviKI_1 RGCY 9 cut(s) 35, 84, 155, 242, 252, 298, 330, 382, 417
CviQI GTAC 3 cut(s) 222, 303, 409
DdeI CTNAG 2 cut(s) 64, 180
DpnI GATC 2 cut(s) 174, 350
DpnII GATC 2 cut(s) 172, 348
DraI TTTAAA 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 332
FaeI CATG 3 cut(s) 58, 128, 355
FaiI YATR 9 cut(s) 17, 56, 114, 126, 336, 353, 402, 420, 473
FatI CATG 3 cut(s) 54, 124, 351
FokI GGATG 2 cut(s) 160, 357
FriOI GRGCYC 1 cut(s) 332
FspBI CTAG 1 cut(s) 78
FspI TGCGCA 1 cut(s) 25
GlaI GCGC 1 cut(s) 25
HaeIII GGCC 1 cut(s) 252
HhaI GCGC 1 cut(s) 26
Hin1II CATG 3 cut(s) 58, 128, 355
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 1 cut(s) 230
HphI GGTGA 2 cut(s) 206, 331
Hpy188I TCNGA 4 cut(s) 48, 183, 393, 483
Hpy188III TCNNGA 2 cut(s) 78, 352
HpyAV CCTTC 2 cut(s) 81, 349
HpyCH4III ACNGT 1 cut(s) 226
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 2 cut(s) 64, 180
Hsp92II CATG 3 cut(s) 58, 128, 355
HspAI GCGC 1 cut(s) 24
Kzo9I GATC 2 cut(s) 172, 348
LmnI GCTCC 2 cut(s) 327, 379
LpnPI CCDG 6 cut(s) 189, 226, 255, 256, 324, 465
MaeI CTAG 1 cut(s) 78
MalI GATC 2 cut(s) 174, 350
MboI GATC 2 cut(s) 172, 348
MboII GAAGA 3 cut(s) 81, 112, 350
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 1 cut(s) 279
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 8 cut(s) 24, 73, 74, 85, 263, 273, 316, 370
MseI TTAA 2 cut(s) 107, 167
MslI CAYNNNNRTG 1 cut(s) 192
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeII GATC 2 cut(s) 172, 348
NlaIII CATG 3 cut(s) 58, 128, 355
NlaIV GGNNCC 2 cut(s) 211, 329
NsbI TGCGCA 1 cut(s) 25
OliI CACNNNNGTG 1 cut(s) 192
PagI TCATGA 1 cut(s) 351
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 211, 329
PspPI GGNCC 1 cut(s) 250
RsaI GTAC 3 cut(s) 223, 304, 410
RsaNI GTAC 3 cut(s) 222, 303, 409
RseI CAYNNNNRTG 1 cut(s) 192
SaqAI TTAA 2 cut(s) 107, 167
Sau3AI GATC 2 cut(s) 172, 348
Sau96I GGNCC 1 cut(s) 250
SchI GAGTC 1 cut(s) 239
SduI GDGCHC 1 cut(s) 332
SetI ASST 6 cut(s) 65, 300, 308, 360, 384, 419
SmiMI CAYNNNNRTG 1 cut(s) 192
Sse9I AATT 1 cut(s) 279
SspMI CTAG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 226
TasI AATT 1 cut(s) 279
Tru1I TTAA 2 cut(s) 107, 167
Tru9I TTAA 2 cut(s) 107, 167
TspDTI ATGAA 5 cut(s) 65, 71, 351, 368, 462
XbaI TCTAGA 1 cut(s) 77
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.