Rroxscaffold_1G00058060

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005002
Physical Location & Seq
Forward (+)
79973400 .. 79975065
1666 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00058060.1

Sequence Viewer

Length: 459 bp
ATGGCTTCAAGGTTGGCTTCAAGGGCTGTTTCATACCTCAGAATCCAAGTGACCCACAGAGGGTTTGCTTCTGTTGTGAAGGATCTCAACTATGCTAATTCTCACGAGTGGGTGAAGGTTGATGGAAAGTCTGCAACTGTTGGCATTACTGATCATGCTCAAGATCATTTAGGTGATGTTGTGTATGTTGAGTTGCCAGAGGTTGGGGTTGCTGTGAAGCAGGGTGATGCGTTTGGAGCAGTTGAAAGTGTCAAGGCTACCAGTGATGTTTACTCTCCTGTGACGGGAAAAGTGGTTGAAGTCAATGAAGAGCTTAGCAGCTCCCCTGGTTTGGTTAACTCAAGCCCGTATGAGAAGGGATGGATAATTAAAGTCGAGATAAATGATAGTGGAGAACTGAAGAACTTGATGGATTCAGACAAGTACACCAAGTTTTGTGAAGAAGAAGATGCACATTGA

Protein Analysis

152

Amino Acids

16.54

Weight (kDa)

4.95

Isoelectric Point (pI)

32.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 29 - 148 6.5e-50 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 203
AclWI GGATC 1 cut(s) 90
AcuI CTGAAG 1 cut(s) 419
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 4 cut(s) 60, 203, 284, 331
AgsI TTSAA 4 cut(s) 9, 21, 245, 299
AjnI CCWGG 1 cut(s) 325
AluBI AGCT 2 cut(s) 313, 321
AluI AGCT 2 cut(s) 313, 321
AlwI GGATC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 318
AsuHPI GGTGA 3 cut(s) 124, 185, 236
BauI CACGAG 1 cut(s) 104
BbvI GCAGC 1 cut(s) 330
BccI CCATC 3 cut(s) 116, 354, 403
BciT130I CCWGG 1 cut(s) 327
BclI TGATCA 1 cut(s) 151
BisI GCNGC 1 cut(s) 319
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 320
Bme1390I CCNGG 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 2 cut(s) 217, 439
Bpu1102I GCTNAGC 1 cut(s) 314
BpuEI CTTGAG 2 cut(s) 144, 325
BsaJI CCNNGG 1 cut(s) 325
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 203, 284, 331
Bse1I ACTGG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 1 cut(s) 365
BseLI CCNNNNNNNGG 4 cut(s) 60, 203, 284, 331
BseMII CTCAG 1 cut(s) 52
BseNI ACTGG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 330
BslI CCNNNNNNNGG 4 cut(s) 60, 203, 284, 331
Bsp143I GATC 3 cut(s) 82, 151, 163
Bsp1720I GCTNAGC 1 cut(s) 314
BspCNI CTCAG 1 cut(s) 51
BspPI GGATC 1 cut(s) 90
BspQI GCTCTTC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 261
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 3 cut(s) 82, 151, 163
BssSI CACGAG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 303
BstDEI CTNAG 2 cut(s) 38, 314
BstF5I GGATG 1 cut(s) 365
BstKTI GATC 3 cut(s) 85, 154, 166
BstMBI GATC 3 cut(s) 82, 151, 163
BstMWI GCNNNNNNNGC 2 cut(s) 23, 236
BstNI CCWGG 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 325
BstV1I GCAGC 1 cut(s) 330
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BtsCI GGATG 1 cut(s) 365
BtsIMutI CAGTG 1 cut(s) 268
Csp6I GTAC 1 cut(s) 424
CviAII CATG 1 cut(s) 155
CviJI RGCY 7 cut(s) 5, 17, 26, 257, 313, 321, 345
CviKI_1 RGCY 7 cut(s) 5, 17, 26, 257, 313, 321, 345
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 2 cut(s) 38, 314
DpnI GATC 3 cut(s) 84, 153, 165
DpnII GATC 3 cut(s) 82, 151, 163
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco57I CTGAAG 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 325
FaeI CATG 1 cut(s) 158
FaiI YATR 5 cut(s) 34, 93, 156, 186, 351
FalI AAGNNNNNCTT 1 cut(s) 33
FatI CATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 319
FokI GGATG 1 cut(s) 372
Fsp4HI GCNGC 1 cut(s) 319
GluI GCNGC 1 cut(s) 319
Hin1II CATG 1 cut(s) 158
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 2 cut(s) 42, 413
HpaI GTTAAC 1 cut(s) 337
HphI GGTGA 3 cut(s) 124, 185, 236
Hpy166II GTNNAC 3 cut(s) 271, 337, 426
Hpy188I TCNGA 2 cut(s) 41, 418
Hpy188III TCNNGA 3 cut(s) 104, 161, 376
Hpy8I GTNNAC 3 cut(s) 271, 337, 426
HpyAV CCTTC 3 cut(s) 73, 109, 349
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 2 cut(s) 134, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 236
HpyF3I CTNAG 2 cut(s) 38, 314
Hsp92II CATG 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 151
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 3 cut(s) 82, 151, 163
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 2 cut(s) 236, 326
LpnPI CCDG 6 cut(s) 206, 210, 274, 291, 312, 339
Lsp1109I GCAGC 1 cut(s) 330
LweI GCATC 2 cut(s) 217, 439
MaeIII GTNAC 2 cut(s) 49, 280
MalI GATC 3 cut(s) 84, 153, 165
MboI GATC 3 cut(s) 82, 151, 163
MboII GAAGA 5 cut(s) 320, 412, 452, 455, 458
MflI RGATCY 1 cut(s) 82
MluCI AATT 2 cut(s) 97, 366
MnlI CCTC 3 cut(s) 47, 53, 193
MseI TTAA 2 cut(s) 336, 369
MslI CAYNNNNRTG 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 2 cut(s) 23, 236
NdeII GATC 3 cut(s) 82, 151, 163
NlaIII CATG 1 cut(s) 158
NmuCI GTSAC 2 cut(s) 49, 280
PciSI GCTCTTC 1 cut(s) 303
PfeI GAWTC 2 cut(s) 42, 413
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 82
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 336, 369
SatI GCNGC 1 cut(s) 319
Sau3AI GATC 3 cut(s) 82, 151, 163
ScrFI CCNGG 1 cut(s) 327
SetI ASST 7 cut(s) 14, 39, 120, 175, 204, 315, 323
SfaNI GCATC 2 cut(s) 217, 439
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 2 cut(s) 159, 340
SmoI CTYRAG 2 cut(s) 159, 340
Sse9I AATT 2 cut(s) 97, 366
StyD4I CCNGG 1 cut(s) 325
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 375
TasI AATT 2 cut(s) 97, 366
TatI WGTACW 1 cut(s) 423
TfiI GAWTC 2 cut(s) 42, 413
Tru1I TTAA 2 cut(s) 336, 369
Tru9I TTAA 2 cut(s) 336, 369
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 2 cut(s) 49, 280
TseI GCWGC 1 cut(s) 318
Tsp45I GTSAC 2 cut(s) 49, 280
TspDTI ATGAA 2 cut(s) 21, 321
TspRI CASTG 1 cut(s) 268
Van91I CCANNNNNTGG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.