AT2G41410

calcium-binding protein CML35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007328
Physical Location & Seq
Reverse (-)
17261728 .. 17263017
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G41410.1

Sequence Viewer

Length: 651 bp
ATGAAGCTCGCCGCTAGCCTCAACCGCCTCAGCCCCAAACGTCTCTTCCGTACCAAATCAAAAGCTTCCGTCTCCAGATCTGAGCCTTCTTCCTTCAGCTCCAATGCTTCTTCCTCTTCCTCTGATGGATCTTACGGTAACCTCAAACAAGGTCCAACCGCGACTCCGATCAGTGTTCTCCCTCAAAACTCCGGCGATTTTTACACTGAGCTTGTTCAAGCTTTTAAACTGATCGATCGTGATGACGACGGTGTTGTTTCCAGAGGAGATCTCGCGGCGTTGATTAGCAGGTTAAGTCATGAACCACCGAGTCAAGAAGAGGTGAGTTTGATGCTAAGAGAAGTAGACGGCGGAGACGGTGGTTGTATCAGCCTTGAAGATCTTGCTAGCCGTGTCGCTGGTACTTCCGGTGAAGGCTCTGTTGAGACGGAGGAGCTGAGAGAGGTGTTCGAGATTTTTGACGTGGATCGTAACGGAAAAATATCGGCGGAGGAGTTACACAGAGTTTTTGGAGTGATCGGAGATGAACGGTGCACGTTAGAAGAGTGTATGCGTATGATAGCGACGGTTGATGGAAACGGTGACGGTTTTGTTTGCTTCGATGACTTTTGCCGCATGATGGTTCCAGCGATGAATGATCATCATCATTAG

Protein Analysis

216

Amino Acids

23.44

Weight (kDa)

4.74

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 70 - 130 5e-09 EF-hand domain pair
EF-hand_7 PF13499 144 - 207 2.2e-11 EF-hand domain pair
EF-hand_1 PF00036 145 - 170 6e-07 EF hand domain
EF-hand_6 PF13405 145 - 172 2.5e-07 EF-hand domain
EF-hand_5 PF13202 146 - 169 8.9e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccI GTMKAC 1 cut(s) 345
AccII CGCG 2 cut(s) 161, 275
AciI CCGC 7 cut(s) 12, 25, 159, 275, 351, 488, 613
AclWI GGATC 2 cut(s) 136, 474
AcuI CTGAAG 1 cut(s) 79
AfaI GTAC 2 cut(s) 52, 403
AfiI CCNNNNNNNGG 1 cut(s) 619
AgsI TTSAA 2 cut(s) 218, 377
AjiI CACGTC 1 cut(s) 463
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 6 cut(s) 7, 65, 99, 211, 221, 436
AluI AGCT 6 cut(s) 7, 65, 99, 211, 221, 436
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 4 cut(s) 47, 76, 348, 419
Alw44I GTGCAC 1 cut(s) 532
AlwI GGATC 2 cut(s) 136, 474
ApaLI GTGCAC 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 152
AsuHPI GGTGA 3 cut(s) 334, 422, 593
AsuNHI GCTAGC 2 cut(s) 14, 386
AvaII GGWCC 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 536
BbvCI CCTCAGC 1 cut(s) 29
BccI CCATC 3 cut(s) 119, 566, 613
BceAI ACGGC 2 cut(s) 364, 375
BclI TGATCA 1 cut(s) 637
BcoDI GTCTC 4 cut(s) 47, 76, 348, 419
BfaI CTAG 2 cut(s) 15, 387
BfuAI ACCTGC 1 cut(s) 279
BglII AGATCT 3 cut(s) 77, 268, 379
BisI GCNGC 3 cut(s) 12, 276, 613
BlsI GCNGC 3 cut(s) 13, 277, 614
Bme18I GGWCC 1 cut(s) 152
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 624
BmsI GCATC 1 cut(s) 321
BmtI GCTAGC 2 cut(s) 18, 390
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 1 cut(s) 58
Bpu10I CCTNAGC 1 cut(s) 29
Bsa29I ATCGAT 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 642
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 2 cut(s) 148, 178
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse8I GATNNNNATC 1 cut(s) 642
BseCI ATCGAT 1 cut(s) 234
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMII CTCAG 4 cut(s) 43, 72, 198, 428
BseRI GAGGAG 3 cut(s) 279, 446, 506
BseSI GKGCMC 1 cut(s) 536
Bsh1236I CGCG 2 cut(s) 161, 275
Bsh1285I CGRYCG 1 cut(s) 238
BshVI ATCGAT 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 536
BsiSI CCGG 2 cut(s) 192, 408
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 4 cut(s) 47, 76, 348, 419
BsmBI CGTCTC 4 cut(s) 47, 76, 348, 419
Bsp1286I GDGCHC 1 cut(s) 536
BspACI CCGC 7 cut(s) 12, 25, 159, 275, 351, 488, 613
BspCNI CTCAG 4 cut(s) 42, 73, 199, 429
BspDI ATCGAT 1 cut(s) 234
BspFNI CGCG 2 cut(s) 161, 275
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 624
BspMI ACCTGC 1 cut(s) 279
BspOI GCTAGC 2 cut(s) 18, 390
BspPI GGATC 2 cut(s) 136, 474
Bst4CI ACNGT 7 cut(s) 137, 251, 359, 531, 568, 581, 587
Bst6I CTCTTC 4 cut(s) 50, 121, 312, 537
BstC8I GCNNGC 3 cut(s) 9, 16, 388
BstDEI CTNAG 5 cut(s) 29, 81, 207, 335, 437
BstEII GGTNACC 1 cut(s) 137
BstFNI CGCG 2 cut(s) 161, 275
BstMAI GTCTC 4 cut(s) 47, 76, 348, 419
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 536
BstUI CGCG 2 cut(s) 161, 275
BstX2I RGATCY 4 cut(s) 77, 128, 268, 379
BstYI RGATCY 4 cut(s) 77, 128, 268, 379
Bsu15I ATCGAT 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 234
BtgZI GCGATG 1 cut(s) 644
BtrI CACGTC 1 cut(s) 463
BtsIMutI CAGTG 2 cut(s) 178, 204
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 3 cut(s) 9, 16, 388
CciI TCATGA 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 152
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 2 cut(s) 51, 402
CviAII CATG 2 cut(s) 299, 616
CviQI GTAC 2 cut(s) 51, 402
DdeI CTNAG 5 cut(s) 29, 81, 207, 335, 437
DraI TTTAAA 1 cut(s) 226
Eam1104I CTCTTC 4 cut(s) 50, 121, 312, 537
EarI CTCTTC 4 cut(s) 50, 121, 312, 537
EciI GGCGGA 2 cut(s) 366, 503
Eco47I GGWCC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 79
Eco91I GGTNACC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 137
Esp3I CGTCTC 4 cut(s) 47, 76, 348, 419
FaeI CATG 2 cut(s) 302, 619
FaiI YATR 4 cut(s) 300, 551, 557, 617
FatI CATG 2 cut(s) 298, 615
FbaI TGATCA 1 cut(s) 637
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 3 cut(s) 12, 276, 613
Fsp4HI GCNGC 3 cut(s) 12, 276, 613
FspBI CTAG 2 cut(s) 15, 387
GluI GCNGC 3 cut(s) 12, 276, 613
GsuI CTGGAG 1 cut(s) 58
HapII CCGG 2 cut(s) 192, 408
Hin1II CATG 2 cut(s) 302, 619
HindIII AAGCTT 2 cut(s) 63, 219
HinfI GANTC 2 cut(s) 163, 310
HpaII CCGG 2 cut(s) 192, 408
HphI GGTGA 3 cut(s) 334, 422, 593
Hpy166II GTNNAC 2 cut(s) 346, 534
Hpy188I TCNGA 4 cut(s) 82, 124, 168, 521
Hpy188III TCNNGA 6 cut(s) 75, 239, 261, 299, 314, 451
Hpy8I GTNNAC 2 cut(s) 346, 534
Hpy99I CGWCG 2 cut(s) 251, 568
HpyAV CCTTC 3 cut(s) 96, 103, 407
HpyCH4III ACNGT 7 cut(s) 137, 251, 359, 531, 568, 581, 587
HpyCH4IV ACGT 3 cut(s) 40, 462, 536
HpyCH4V TGCA 1 cut(s) 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 5 cut(s) 29, 81, 207, 335, 437
HpySE526I ACGT 3 cut(s) 40, 462, 536
Hsp92II CATG 2 cut(s) 302, 619
Ksp22I TGATCA 1 cut(s) 637
LmnI GCTCC 2 cut(s) 104, 433
LpnPI CCDG 7 cut(s) 88, 205, 274, 274, 384, 421, 639
LweI GCATC 1 cut(s) 321
MaeI CTAG 2 cut(s) 15, 387
MaeII ACGT 3 cut(s) 40, 462, 536
MaeIII GTNAC 4 cut(s) 137, 470, 495, 581
MboII GAAGA 7 cut(s) 37, 81, 102, 108, 329, 389, 554
MflI RGATCY 4 cut(s) 77, 128, 268, 379
MhlI GDGCHC 1 cut(s) 536
MlyI GAGTC 2 cut(s) 157, 319
MmeI TCCRAC 1 cut(s) 179
MseI TTAA 2 cut(s) 225, 293
MspI CCGG 2 cut(s) 192, 408
MvnI CGCG 2 cut(s) 161, 275
MwoI GCNNNNNNNGC 1 cut(s) 24
NheI GCTAGC 2 cut(s) 14, 386
NlaIII CATG 2 cut(s) 302, 619
NlaIV GGNNCC 1 cut(s) 624
NmuCI GTSAC 1 cut(s) 581
PagI TCATGA 1 cut(s) 298
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PkrI GCNGC 3 cut(s) 13, 277, 614
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 2 cut(s) 157, 318
PpsI GAGTC 2 cut(s) 157, 318
PspEI GGTNACC 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 624
PspPI GGNCC 1 cut(s) 152
PsuI RGATCY 4 cut(s) 77, 128, 268, 379
PvuI CGATCG 1 cut(s) 238
RsaI GTAC 2 cut(s) 52, 403
RsaNI GTAC 2 cut(s) 51, 402
SaqAI TTAA 2 cut(s) 225, 293
SatI GCNGC 3 cut(s) 12, 276, 613
Sau96I GGNCC 1 cut(s) 152
SchI GAGTC 2 cut(s) 157, 319
SduI GDGCHC 1 cut(s) 536
SfaNI GCATC 1 cut(s) 321
SinI GGWCC 1 cut(s) 152
SsiI CCGC 7 cut(s) 12, 25, 159, 275, 351, 488, 613
SspMI CTAG 2 cut(s) 15, 387
TaaI ACNGT 7 cut(s) 137, 251, 359, 531, 568, 581, 587
TaiI ACGT 3 cut(s) 43, 465, 539
TaqI TCGA 3 cut(s) 234, 450, 600
TauI GCSGC 3 cut(s) 14, 278, 615
Tru1I TTAA 2 cut(s) 225, 293
Tru9I TTAA 2 cut(s) 225, 293
TscAI CASTG 2 cut(s) 178, 211
TseFI GTSAC 1 cut(s) 581
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 4 cut(s) 17, 315, 540, 647
TspGWI ACGGA 4 cut(s) 38, 58, 443, 489
TspRI CASTG 2 cut(s) 178, 211
VneI GTGCAC 1 cut(s) 532
VpaK11BI GGWCC 1 cut(s) 152
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 2 cut(s) 15, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.