pycom07g14940

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007328
Physical Location & Seq
Reverse (-)
16974190 .. 16975029
840 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g14940.1

Sequence Viewer

Length: 840 bp
ATGGCAGTGCCTTCACTAACACCATATATCCCCCCATCTCTTCCTCTCTGCTTCCCATCTTTTCCATTTTCTCTCCTCCTTTTTCACCATTATCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACAACTTCTTCGCCCATTTGGGTTTTCATCTTCAATCATGAAGTTCATCAATATAAGCTCCAAAAATCTCAGTCCGAAACGCCTCTTCCGATCTAAGAAGGACCGGTACGCCGTGTCCAGCTCCGATCCGCCATCGTTCGGGTCCGGAACGGATTTTTCCTCATCTTTTTCTTCTTACTCAGACTCAATTCACAAGCCCGGGGCCGGTGGTGTCACCACGCCCACCAGCGTTTTGGCCGAAAGATCGGGTTACTGGTCCGAGTTTTCGGCTGATCTGCAGCTGGACATGGCCAAGGCGTTTAAGCTTATAGACCGGGACAACGACGGAGTGGTGTCGAGGAAAGAACTCGAGGCACTTCTGTGCCAGCTCGGGAACAACCCGCCGAGTCAAGAGGAGGTGACGTTGATGCTGAGCGAGGTGGACCGAGACGGCAGCGGTTCGATAACCCTTGAGGCTTTATTGAACCAGGTCGGACCGGTTTCCGGTCCACCTGCGGACTCGGAGCTGCGCGATGCGTTCGAAGTATTTGACACGGATAACGACGGCAAAATATCGGCGGAGGAACTTTTGAGGGTTTTCAAGGCCATTGGGGACGAACTGTGCACGTTAGAGGAGTGCCGGCGCATGATAGCCGAGGTAGATAAAAACGGGGATGGGTTCGTGTGCTTTGAGGAGTTTGTTCATATGATGGAGCTGCAGAGATGA

Protein Analysis

280

Amino Acids

30.69

Weight (kDa)

4.77

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 140 - 196 1e-10 EF-hand domain pair
EF-hand_6 PF13405 141 - 169 3.7e-06 EF-hand domain
EF-hand_7 PF13499 212 - 275 8e-15 EF-hand domain pair
EF-hand_1 PF00036 213 - 240 1.3e-08 EF hand domain
EF-hand_6 PF13405 213 - 242 9.7e-09 EF-hand domain
EF-hand_5 PF13202 214 - 237 3.2e-08 EF hand
EF-hand_1 PF00036 250 - 276 2.1e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 634
Acc36I ACCTGC 1 cut(s) 634
AccII CGCG 1 cut(s) 645
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 5 cut(s) 263, 515, 570, 629, 692
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 2 cut(s) 369, 423
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 3 cut(s) 272, 338, 617
AgeI ACCGGT 2 cut(s) 237, 610
AgsI TTSAA 3 cut(s) 168, 598, 715
AjnI CCWGG 1 cut(s) 600
AleI CACNNNNGTG 1 cut(s) 493
AluBI AGCT 7 cut(s) 192, 255, 415, 439, 502, 640, 829
AluI AGCT 7 cut(s) 192, 255, 415, 439, 502, 640, 829
Alw21I GWGCWC 1 cut(s) 740
Alw26I GTCTC 1 cut(s) 555
Alw44I GTGCAC 1 cut(s) 736
AlwI GGATC 1 cut(s) 254
Ama87I CYCGRG 3 cut(s) 332, 482, 503
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 4 cut(s) 336, 369, 423, 717
ApaLI GTGCAC 1 cut(s) 736
ApeKI GCWGC 4 cut(s) 412, 567, 640, 829
AsiGI ACCGGT 2 cut(s) 237, 610
AspLEI GCGC 2 cut(s) 645, 759
AspS9I GGNCC 7 cut(s) 235, 276, 336, 390, 556, 608, 620
AsuC2I CCSGG 3 cut(s) 333, 334, 449
AsuHPI GGTGA 3 cut(s) 77, 340, 544
AsuII TTCGAA 1 cut(s) 654
AvaI CYCGRG 3 cut(s) 332, 482, 503
AvaII GGWCC 6 cut(s) 235, 276, 390, 556, 608, 620
BaeGI GKGCMC 1 cut(s) 740
BalI TGGCCA 1 cut(s) 425
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
Bbv12I GWGCWC 1 cut(s) 740
BbvI GCAGC 4 cut(s) 424, 579, 627, 816
BccI CCATC 5 cut(s) 43, 64, 274, 782, 817
BceAI ACGGC 3 cut(s) 230, 580, 694
BciT130I CCWGG 1 cut(s) 602
BcnI CCSGG 3 cut(s) 333, 334, 449
BcoDI GTCTC 1 cut(s) 555
BfmI CTRYAG 2 cut(s) 410, 830
BfuAI ACCTGC 1 cut(s) 634
BisI GCNGC 4 cut(s) 413, 568, 641, 830
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 4 cut(s) 414, 569, 642, 831
Bme1390I CCNGG 4 cut(s) 333, 334, 449, 602
Bme18I GGWCC 6 cut(s) 235, 276, 390, 556, 608, 620
BmeT110I CYCGRG 3 cut(s) 332, 482, 503
BmgT120I GGNCC 7 cut(s) 235, 276, 336, 390, 556, 608, 620
BmiI GGNNCC 2 cut(s) 277, 337
BmrFI CCNGG 4 cut(s) 333, 334, 449, 602
BmsI GCATC 2 cut(s) 531, 637
Bpu1102I GCTNAGC 1 cut(s) 545
Bpu14I TTCGAA 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 3 cut(s) 333, 334, 449
BsaJI CCNNGG 4 cut(s) 332, 333, 426, 768
BsaWI WCCGGW 4 cut(s) 237, 278, 610, 617
BsaXI ACNNNNNCTCC 2 cut(s) 521, 551
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 338, 617
Bse118I RCCGGY 4 cut(s) 237, 338, 610, 753
Bse1I ACTGG 1 cut(s) 392
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 4 cut(s) 332, 333, 426, 768
BseGI GGATG 1 cut(s) 793
BseLI CCNNNNNNNGG 3 cut(s) 272, 338, 617
BseMII CTCAG 3 cut(s) 217, 327, 536
BseNI ACTGG 1 cut(s) 392
BseRI GAGGAG 4 cut(s) 65, 542, 761, 821
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 4 cut(s) 424, 579, 627, 816
Bsh1236I CGCG 1 cut(s) 645
BshFI GGCC 4 cut(s) 338, 371, 425, 719
BshTI ACCGGT 2 cut(s) 237, 610
BsiHKAI GWGCWC 1 cut(s) 740
BsiHKCI CYCGRG 3 cut(s) 332, 482, 503
BsiSI CCGG 8 cut(s) 238, 279, 333, 339, 448, 611, 618, 754
BslFI GGGAC 2 cut(s) 464, 740
BslI CCNNNNNNNGG 3 cut(s) 272, 338, 617
BsmAI GTCTC 1 cut(s) 555
BsmBI CGTCTC 1 cut(s) 555
BsmFI GGGAC 2 cut(s) 464, 740
BsnI GGCC 4 cut(s) 338, 371, 425, 719
BsoBI CYCGRG 3 cut(s) 332, 482, 503
Bsp119I TTCGAA 1 cut(s) 654
Bsp1286I GDGCHC 1 cut(s) 740
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 4 cut(s) 224, 259, 377, 406
Bsp1720I GCTNAGC 1 cut(s) 545
BspACI CCGC 5 cut(s) 263, 515, 570, 629, 692
BspANI GGCC 4 cut(s) 338, 371, 425, 719
BspCNI CTCAG 3 cut(s) 216, 326, 537
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 1 cut(s) 645
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 277, 337
BspMAI CTGCAG 2 cut(s) 414, 834
BspMI ACCTGC 1 cut(s) 634
BspPI GGATC 1 cut(s) 254
BspT104I TTCGAA 1 cut(s) 654
BsrFI RCCGGY 4 cut(s) 237, 338, 610, 753
BsrI ACTGG 1 cut(s) 392
BssAI RCCGGY 4 cut(s) 237, 338, 610, 753
BssECI CCNNGG 4 cut(s) 332, 333, 426, 768
BssMI GATC 4 cut(s) 224, 259, 377, 406
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 1 cut(s) 735
Bst6I CTCTTC 2 cut(s) 45, 224
BstBI TTCGAA 1 cut(s) 654
BstC8I GCNNGC 2 cut(s) 500, 755
BstDEI CTNAG 4 cut(s) 203, 228, 313, 545
BstF5I GGATG 1 cut(s) 793
BstFNI CGCG 1 cut(s) 645
BstHHI GCGC 2 cut(s) 645, 759
BstKTI GATC 4 cut(s) 227, 262, 380, 409
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 4 cut(s) 224, 259, 377, 406
BstNI CCWGG 1 cut(s) 602
BstSCI CCNGG 4 cut(s) 331, 332, 447, 600
BstSFI CTRYAG 2 cut(s) 410, 830
BstSLI GKGCMC 1 cut(s) 740
BstUI CGCG 1 cut(s) 645
BstV1I GCAGC 4 cut(s) 424, 579, 627, 816
BstXI CCANNNNNNTGG 1 cut(s) 367
BsuRI GGCC 4 cut(s) 338, 371, 425, 719
BtgZI GCGATG 1 cut(s) 660
BtsCI GGATG 1 cut(s) 793
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 12
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 2 cut(s) 500, 755
CciI TCATGA 1 cut(s) 171
CfoI GCGC 2 cut(s) 645, 759
Cfr10I RCCGGY 4 cut(s) 237, 338, 610, 753
Cfr13I GGNCC 7 cut(s) 235, 276, 336, 390, 556, 608, 620
Cfr9I CCCGGG 1 cut(s) 332
CpoI CGGWCCG 1 cut(s) 608
CsiI ACCWGGT 1 cut(s) 600
Csp6I GTAC 1 cut(s) 241
CspAI ACCGGT 2 cut(s) 237, 610
CspI CGGWCCG 1 cut(s) 608
CviAII CATG 3 cut(s) 172, 421, 760
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 4 cut(s) 203, 228, 313, 545
DpnI GATC 4 cut(s) 226, 261, 379, 408
DpnII GATC 4 cut(s) 224, 259, 377, 406
EaeI YGGCCR 2 cut(s) 369, 423
Eam1104I CTCTTC 2 cut(s) 45, 224
EarI CTCTTC 2 cut(s) 45, 224
EciI GGCGGA 2 cut(s) 252, 707
Eco130I CCWWGG 1 cut(s) 426
Eco47I GGWCC 6 cut(s) 235, 276, 390, 556, 608, 620
Eco88I CYCGRG 3 cut(s) 332, 482, 503
EcoRII CCWGG 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 426
Esp3I CGTCTC 1 cut(s) 555
FaeI CATG 3 cut(s) 175, 424, 763
FaiI YATR 9 cut(s) 25, 27, 173, 188, 422, 443, 761, 819, 821
FaqI GGGAC 2 cut(s) 464, 740
FatI CATG 3 cut(s) 171, 420, 759
FauI CCCGC 1 cut(s) 522
FauNDI CATATG 1 cut(s) 819
Fnu4HI GCNGC 4 cut(s) 413, 568, 641, 830
FokI GGATG 1 cut(s) 800
Fsp4HI GCNGC 4 cut(s) 413, 568, 641, 830
GlaI GCGC 2 cut(s) 644, 758
GluI GCNGC 4 cut(s) 413, 568, 641, 830
HaeIII GGCC 4 cut(s) 338, 371, 425, 719
HapII CCGG 8 cut(s) 238, 279, 333, 339, 448, 611, 618, 754
HhaI GCGC 2 cut(s) 645, 759
Hin1II CATG 3 cut(s) 175, 424, 763
Hin6I GCGC 2 cut(s) 643, 757
HinP1I GCGC 2 cut(s) 643, 757
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 3 cut(s) 317, 520, 632
HpaII CCGG 8 cut(s) 238, 279, 333, 339, 448, 611, 618, 754
HphI GGTGA 3 cut(s) 77, 340, 544
Hpy166II GTNNAC 3 cut(s) 556, 623, 738
Hpy188I TCNGA 7 cut(s) 210, 224, 259, 316, 394, 608, 637
Hpy188III TCNNGA 4 cut(s) 172, 279, 505, 524
Hpy8I GTNNAC 3 cut(s) 556, 623, 738
Hpy99I CGWCG 2 cut(s) 461, 680
HpyAV CCTTC 2 cut(s) 21, 226
HpyCH4III ACNGT 1 cut(s) 735
HpyCH4IV ACGT 2 cut(s) 536, 740
HpyCH4V TGCA 3 cut(s) 412, 738, 832
HpyF3I CTNAG 4 cut(s) 203, 228, 313, 545
HpySE526I ACGT 2 cut(s) 536, 740
Hsp92II CATG 3 cut(s) 175, 424, 763
HspAI GCGC 2 cut(s) 643, 757
Kpn2I TCCGGA 1 cut(s) 278
KroI GCCGGC 1 cut(s) 753
KroNI GCCGGC 1 cut(s) 755
Kzo9I GATC 4 cut(s) 224, 259, 377, 406
LmnI GCTCC 4 cut(s) 197, 260, 637, 826
Lsp1109I GCAGC 4 cut(s) 424, 579, 627, 816
LweI GCATC 2 cut(s) 531, 637
MabI ACCWGGT 1 cut(s) 600
MaeII ACGT 2 cut(s) 536, 740
MaeIII GTNAC 3 cut(s) 346, 383, 532
MalI GATC 4 cut(s) 226, 261, 379, 408
MboI GATC 4 cut(s) 224, 259, 377, 406
MboII GAAGA 6 cut(s) 32, 86, 134, 156, 211, 297
MhlI GDGCHC 1 cut(s) 740
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 1 cut(s) 321
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 3 cut(s) 311, 529, 626
MmeI TCCRAC 1 cut(s) 586
Mox20I TGGCCA 1 cut(s) 425
MroI TCCGGA 1 cut(s) 278
MroNI GCCGGC 1 cut(s) 753
MscI TGGCCA 1 cut(s) 425
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 1 cut(s) 493
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 415, 570
MspI CCGG 8 cut(s) 238, 279, 333, 339, 448, 611, 618, 754
MspR9I CCNGG 4 cut(s) 333, 334, 449, 602
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 1 cut(s) 645
NaeI GCCGGC 1 cut(s) 755
NciI CCSGG 3 cut(s) 333, 334, 449
NdeI CATATG 1 cut(s) 819
NdeII GATC 4 cut(s) 224, 259, 377, 406
NgoMIV GCCGGC 1 cut(s) 753
NlaIII CATG 3 cut(s) 175, 424, 763
NlaIV GGNNCC 2 cut(s) 277, 337
NmeAIII GCCGAG 2 cut(s) 543, 793
NmuCI GTSAC 2 cut(s) 346, 532
NspV TTCGAA 1 cut(s) 654
OliI CACNNNNGTG 1 cut(s) 493
PaeR7I CTCGAG 1 cut(s) 482
PagI TCATGA 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PdiI GCCGGC 1 cut(s) 755
PinAI ACCGGT 2 cut(s) 237, 610
PkrI GCNGC 4 cut(s) 414, 569, 642, 831
PleI GAGTC 3 cut(s) 311, 528, 626
PpsI GAGTC 3 cut(s) 311, 528, 626
Psp6I CCWGG 1 cut(s) 600
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 2 cut(s) 277, 337
PspPI GGNCC 7 cut(s) 235, 276, 336, 390, 556, 608, 620
PspXI VCTCGAGB 1 cut(s) 482
PstI CTGCAG 2 cut(s) 414, 834
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 493
Rsr2I CGGWCCG 1 cut(s) 608
RsrII CGGWCCG 1 cut(s) 608
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 4 cut(s) 413, 568, 641, 830
Sau3AI GATC 4 cut(s) 224, 259, 377, 406
Sau96I GGNCC 7 cut(s) 235, 276, 336, 390, 556, 608, 620
SchI GAGTC 3 cut(s) 311, 529, 626
ScrFI CCNGG 4 cut(s) 333, 334, 449, 602
SduI GDGCHC 1 cut(s) 740
SexAI ACCWGGT 1 cut(s) 600
SfaNI GCATC 2 cut(s) 531, 637
SfcI CTRYAG 2 cut(s) 410, 830
Sfr274I CTCGAG 1 cut(s) 482
SfuI TTCGAA 1 cut(s) 654
SinI GGWCC 6 cut(s) 235, 276, 390, 556, 608, 620
SlaI CTCGAG 1 cut(s) 482
SmaI CCCGGG 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 493
SmlI CTYRAG 2 cut(s) 482, 584
SmoI CTYRAG 2 cut(s) 482, 584
Sse9I AATT 1 cut(s) 321
SsiI CCGC 5 cut(s) 263, 515, 570, 629, 692
StyD4I CCNGG 4 cut(s) 331, 332, 447, 600
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 735
TaiI ACGT 2 cut(s) 539, 743
TaqI TCGA 4 cut(s) 470, 483, 575, 654
TaqII GACCGA 1 cut(s) 573
TasI AATT 1 cut(s) 321
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 2 cut(s) 346, 532
TseI GCWGC 4 cut(s) 412, 567, 640, 829
Tsp45I GTSAC 2 cut(s) 346, 532
TspDTI ATGAA 4 cut(s) 150, 169, 188, 806
TspGWI ACGGA 3 cut(s) 299, 474, 683
TspMI CCCGGG 1 cut(s) 332
TspRI CASTG 1 cut(s) 12
VneI GTGCAC 1 cut(s) 736
VpaK11BI GGWCC 6 cut(s) 235, 276, 390, 556, 608, 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XhoI CTCGAG 1 cut(s) 482
XmaI CCCGGG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.