Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007328

Physical Location & Seq

Genomic Coordinates Reverse (-)

10720456 .. 10721127

Gene Structure 672 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1087300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 672 bp

ATGAAGTTCATCAAAATTAGCTCCAAAAATCTCAGTCCGAAACGCCTCTTCCGGTCTAAGAAGGACCGGTCCGCCGTATCTCGCTCCGAACCGCCGTCGTTCGGGTCCGAAACGGCGTCTGCCTCGTCATCCTCTTCCTCATACACAATCCACAAGCCCGGGGCCGGCGCCGGCGGTGGAACCACGCCCACCAGCGTTTTGCCCGAAAGATCGGGTGAGTGGTCCGAGTTTTCGGCTGATCTGCAGCTCGACATGGCGCAAGCGTTTAAACTTATAGACCGGGACAACGATGGAGTGGTGTCGAGGAAAGAACTCGAGGCTCTTCTGTGCCGGCTCGGTAACGACCCGCAGAGTCGAGAGGAGGTGACGTTGATGCTAAGCGAGGTGGACCGAGACGGTAACGGTTCAATCAGCCTCGAGGCTGTGTTGAACCGGGTCGGGCCGGTTTCCGGTCCAGCTGCCGACTCGGAACTGCGGGATGCGTTTGAAGTGTTTGACACGGATAACGACGGAAAAATATCGGCGGAGGAGCTTTTGAGCTTTTTCAGGGCAATCGGCGACGAAGGGTGCACGTTAGAGGAGTGCGGGCGCATGATAGCAGGGGCTGATAAGAATGGGGATGGGTTCGTGTGCTTTGAGGAGTTTGCTCATATGATGGAGCGTCAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0016020 GO:0043167 GO:0043169 GO:0043207 GO:0044464 GO:0046872 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

224

Amino Acids

24.16

Weight (kDa)

4.8

Isoelectric Point (pI)

46.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	84 - 140	2.5e-11	EF-hand domain pair
EF-hand_6	PF13405	85 - 113	2.6e-06	EF-hand domain
EF-hand_7	PF13499	156 - 219	5.6e-13	EF-hand domain pair
EF-hand_1	PF00036	157 - 184	1.3e-08	EF hand domain
EF-hand_6	PF13405	157 - 186	6.5e-09	EF-hand domain
EF-hand_5	PF13202	158 - 181	7.1e-08	EF hand
EF-hand_1	PF00036	195 - 221	3.3e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41410
fragaria_vesca	FvH4_6g25060
malus_domestica	MD12G1087300.v1.1 MD14G1069200.v1.1 MD14G1083200.v1.1
prunus_persica	Prupe.7G047300_v2.0.a1
pyrus_communis	pycom07g14940 pycom12g07550 pycom14g07230
rosa_chinensis	RchiOBHm_Chr2g0124821
rosa_laevigata	RLG00000018825
rosa_multiflora	Rmu_sc0000507.1_g000002 Rmu_sc0002939.1_g000021
rosa_roxburghii	Rroxscaffold_1G00049520 Rroxscaffold_2G00119640
rosa_rugosa	Rorug02G0252900
rosa_samantha	Rh2AG312400 Rh2BG320600 Rh2CG300600 Rh2DG337900 Rh4BG103800 Rh5BG198000 Rh5BG310900 Rh5DG321400
rosa_wichuraiana	Rw2G025230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	416
AccB1I	GGYRCC	1 cut(s)	167
AciI	CCGC	7 cut(s)	72, 92, 174, 347, 475, 524, 585
AcyI	GRCGYC	2 cut(s)	116, 168
AfiI	CCNNNNNNNGG	3 cut(s)	101, 164, 449
AgeI	ACCGGT	1 cut(s)	66
AgsI	TTSAA	3 cut(s)	408, 430, 488
AluBI	AGCT	5 cut(s)	21, 247, 458, 532, 540
AluI	AGCT	5 cut(s)	21, 247, 458, 532, 540
Alw21I	GWGCWC	1 cut(s)	572
Alw26I	GTCTC	1 cut(s)	387
Alw44I	GTGCAC	1 cut(s)	568
AlwNI	CAGNNNCTG	1 cut(s)	605
Ama87I	CYCGRG	3 cut(s)	158, 314, 416
AoxI	GGCC	2 cut(s)	162, 440
ApaLI	GTGCAC	1 cut(s)	568
ApeKI	GCWGC	2 cut(s)	244, 458
AsiGI	ACCGGT	1 cut(s)	66
AspLEI	GCGC	3 cut(s)	170, 259, 591
AspS9I	GGNCC	8 cut(s)	64, 69, 105, 162, 222, 388, 440, 452
AsuC2I	CCSGG	4 cut(s)	159, 160, 281, 434
AsuHPI	GGTGA	2 cut(s)	227, 376
AvaI	CYCGRG	3 cut(s)	158, 314, 416
AvaII	GGWCC	6 cut(s)	64, 69, 105, 222, 388, 452
BaeGI	GKGCMC	1 cut(s)	572
BanI	GGYRCC	1 cut(s)	167
Bbv12I	GWGCWC	1 cut(s)	572
BbvI	GCAGC	2 cut(s)	256, 445
BccI	CCATC	3 cut(s)	284, 614, 649
BceAI	ACGGC	3 cut(s)	59, 79, 129
BcnI	CCSGG	4 cut(s)	159, 160, 281, 434
BcoDI	GTCTC	1 cut(s)	387
BfmI	CTRYAG	1 cut(s)	242
BfoI	RGCGCY	1 cut(s)	171
BisI	GCNGC	2 cut(s)	245, 459
BlpI	GCTNAGC	1 cut(s)	377
BlsI	GCNGC	2 cut(s)	246, 460
Bme1390I	CCNGG	4 cut(s)	159, 160, 281, 434
Bme18I	GGWCC	6 cut(s)	64, 69, 105, 222, 388, 452
BmeT110I	CYCGRG	3 cut(s)	158, 314, 416
BmgT120I	GGNCC	8 cut(s)	64, 69, 105, 162, 222, 388, 440, 452
BmiI	GGNNCC	4 cut(s)	106, 163, 169, 181
BmrFI	CCNGG	4 cut(s)	159, 160, 281, 434
BmsI	GCATC	2 cut(s)	363, 469
Bpu1102I	GCTNAGC	1 cut(s)	377
BpuMI	CCSGG	4 cut(s)	159, 160, 281, 434
BsaHI	GRCGYC	2 cut(s)	116, 168
BsaJI	CCNNGG	2 cut(s)	158, 159
BsaWI	WCCGGW	3 cut(s)	51, 66, 449
BsaXI	ACNNNNNCTCC	2 cut(s)	353, 383
Bsc4I	CCNNNNNNNGG	3 cut(s)	101, 164, 449
Bse118I	RCCGGY	5 cut(s)	66, 164, 170, 330, 442
BseDI	CCNNGG	2 cut(s)	158, 159
BseGI	GGATG	3 cut(s)	128, 484, 625
BseLI	CCNNNNNNNGG	3 cut(s)	101, 164, 449
BseMII	CTCAG	1 cut(s)	46
BseRI	GAGGAG	4 cut(s)	374, 542, 593, 653
BseSI	GKGCMC	1 cut(s)	572
BseXI	GCAGC	2 cut(s)	256, 445
BshFI	GGCC	2 cut(s)	164, 442
BshNI	GGYRCC	1 cut(s)	167
BshTI	ACCGGT	1 cut(s)	66
BsiHKAI	GWGCWC	1 cut(s)	572
BsiHKCI	CYCGRG	3 cut(s)	158, 314, 416
BslFI	GGGAC	1 cut(s)	296
BslI	CCNNNNNNNGG	3 cut(s)	101, 164, 449
BsmAI	GTCTC	1 cut(s)	387
BsmBI	CGTCTC	1 cut(s)	387
BsmFI	GGGAC	1 cut(s)	296
BsnI	GGCC	2 cut(s)	164, 442
BsoBI	CYCGRG	3 cut(s)	158, 314, 416
Bsp1286I	GDGCHC	1 cut(s)	572
Bsp143I	GATC	2 cut(s)	209, 238
Bsp1720I	GCTNAGC	1 cut(s)	377
BspACI	CCGC	7 cut(s)	72, 92, 174, 347, 475, 524, 585
BspANI	GGCC	2 cut(s)	164, 442
BspCNI	CTCAG	1 cut(s)	45
BspLI	GGNNCC	4 cut(s)	106, 163, 169, 181
BspMAI	CTGCAG	1 cut(s)	246
BspQI	GCTCTTC	1 cut(s)	327
BspT107I	GGYRCC	1 cut(s)	167
BsrFI	RCCGGY	5 cut(s)	66, 164, 170, 330, 442
BssAI	RCCGGY	5 cut(s)	66, 164, 170, 330, 442
BssECI	CCNNGG	2 cut(s)	158, 159
BssMI	GATC	2 cut(s)	209, 238
BssNI	GRCGYC	2 cut(s)	116, 168
Bst4CI	ACNGT	2 cut(s)	398, 404
Bst6I	CTCTTC	3 cut(s)	53, 139, 327
BstACI	GRCGYC	2 cut(s)	116, 168
BstC8I	GCNNGC	5 cut(s)	166, 172, 261, 332, 587
BstDEI	CTNAG	3 cut(s)	32, 57, 377
BstF5I	GGATG	3 cut(s)	128, 484, 625
BstH2I	RGCGCY	1 cut(s)	171
BstHHI	GCGC	3 cut(s)	170, 259, 591
BstKTI	GATC	2 cut(s)	212, 241
BstMAI	GTCTC	1 cut(s)	387
BstMBI	GATC	2 cut(s)	209, 238
BstSCI	CCNGG	4 cut(s)	157, 158, 279, 432
BstSFI	CTRYAG	1 cut(s)	242
BstSLI	GKGCMC	1 cut(s)	572
BstV1I	GCAGC	2 cut(s)	256, 445
BsuRI	GGCC	2 cut(s)	164, 442
BtsCI	GGATG	3 cut(s)	128, 484, 625
Cac8I	GCNNGC	5 cut(s)	166, 172, 261, 332, 587
CaiI	CAGNNNCTG	1 cut(s)	605
CfoI	GCGC	3 cut(s)	170, 259, 591
Cfr10I	RCCGGY	5 cut(s)	66, 164, 170, 330, 442
Cfr13I	GGNCC	8 cut(s)	64, 69, 105, 162, 222, 388, 440, 452
Cfr9I	CCCGGG	1 cut(s)	158
CpoI	CGGWCCG	1 cut(s)	69
CseI	GACGC	2 cut(s)	105, 650
CspAI	ACCGGT	1 cut(s)	66
CspI	CGGWCCG	1 cut(s)	69
CviAII	CATG	2 cut(s)	253, 592
DdeI	CTNAG	3 cut(s)	32, 57, 377
DinI	GGCGCC	1 cut(s)	169
DpnI	GATC	2 cut(s)	211, 240
DpnII	GATC	2 cut(s)	209, 238
DraI	TTTAAA	1 cut(s)	268
Eam1104I	CTCTTC	3 cut(s)	53, 139, 327
EarI	CTCTTC	3 cut(s)	53, 139, 327
EciI	GGCGGA	2 cut(s)	61, 539
Eco47I	GGWCC	6 cut(s)	64, 69, 105, 222, 388, 452
Eco88I	CYCGRG	3 cut(s)	158, 314, 416
EgeI	GGCGCC	1 cut(s)	169
EheI	GGCGCC	1 cut(s)	169
Esp3I	CGTCTC	1 cut(s)	387
FaeI	CATG	2 cut(s)	256, 595
FaiI	YATR	6 cut(s)	142, 254, 275, 593, 651, 653
FaqI	GGGAC	1 cut(s)	296
FatI	CATG	2 cut(s)	252, 591
FauI	CCCGC	3 cut(s)	354, 468, 578
FauNDI	CATATG	1 cut(s)	651
Fnu4HI	GCNGC	2 cut(s)	245, 459
FokI	GGATG	3 cut(s)	115, 491, 632
Fsp4HI	GCNGC	2 cut(s)	245, 459
GlaI	GCGC	3 cut(s)	169, 258, 590
GluI	GCNGC	2 cut(s)	245, 459
HaeII	RGCGCY	1 cut(s)	171
HaeIII	GGCC	2 cut(s)	164, 442
HgaI	GACGC	2 cut(s)	105, 650
HhaI	GCGC	3 cut(s)	170, 259, 591
Hin1I	GRCGYC	2 cut(s)	116, 168
Hin1II	CATG	2 cut(s)	256, 595
Hin6I	GCGC	3 cut(s)	168, 257, 589
HinP1I	GCGC	3 cut(s)	168, 257, 589
HinfI	GANTC	2 cut(s)	352, 464
HphI	GGTGA	2 cut(s)	227, 376
Hpy166II	GTNNAC	2 cut(s)	388, 570
Hpy188I	TCNGA	6 cut(s)	39, 88, 109, 226, 469, 666
Hpy188III	TCNNGA	1 cut(s)	356
Hpy8I	GTNNAC	2 cut(s)	388, 570
Hpy99I	CGWCG	3 cut(s)	100, 512, 563
HpyAV	CCTTC	2 cut(s)	55, 557
HpyCH4III	ACNGT	2 cut(s)	398, 404
HpyCH4IV	ACGT	2 cut(s)	368, 572
HpyCH4V	TGCA	2 cut(s)	244, 570
HpyF3I	CTNAG	3 cut(s)	32, 57, 377
HpySE526I	ACGT	2 cut(s)	368, 572
Hsp92I	GRCGYC	2 cut(s)	116, 168
Hsp92II	CATG	2 cut(s)	256, 595
HspAI	GCGC	3 cut(s)	168, 257, 589
KasI	GGCGCC	1 cut(s)	167
KroI	GCCGGC	3 cut(s)	164, 170, 330
KroNI	GCCGGC	3 cut(s)	166, 172, 332
Kzo9I	GATC	2 cut(s)	209, 238
LguI	GCTCTTC	1 cut(s)	327
LmnI	GCTCC	4 cut(s)	26, 89, 529, 658
Lsp1109I	GCAGC	2 cut(s)	256, 445
LweI	GCATC	2 cut(s)	363, 469
MaeII	ACGT	2 cut(s)	368, 572
MaeIII	GTNAC	3 cut(s)	338, 364, 398
MalI	GATC	2 cut(s)	211, 240
MboI	GATC	2 cut(s)	209, 238
MboII	GAAGA	3 cut(s)	40, 126, 314
MhlI	GDGCHC	1 cut(s)	572
MluCI	AATT	1 cut(s)	15
Mly113I	GGCGCC	1 cut(s)	168
MlyI	GAGTC	2 cut(s)	361, 458
MreI	CGCCGGCG	1 cut(s)	170
MroNI	GCCGGC	3 cut(s)	164, 170, 330
MseI	TTAA	1 cut(s)	267
MspA1I	CMGCKG	1 cut(s)	458
MspR9I	CCNGG	4 cut(s)	159, 160, 281, 434
MssI	GTTTAAAC	1 cut(s)	268
NaeI	GCCGGC	3 cut(s)	166, 172, 332
NarI	GGCGCC	1 cut(s)	168
NciI	CCSGG	4 cut(s)	159, 160, 281, 434
NdeI	CATATG	1 cut(s)	651
NdeII	GATC	2 cut(s)	209, 238
NgoMIV	GCCGGC	3 cut(s)	164, 170, 330
NlaIII	CATG	2 cut(s)	256, 595
NlaIV	GGNNCC	4 cut(s)	106, 163, 169, 181
NmuCI	GTSAC	1 cut(s)	364
PaeR7I	CTCGAG	2 cut(s)	314, 416
PciSI	GCTCTTC	1 cut(s)	327
PdiI	GCCGGC	3 cut(s)	166, 172, 332
PinAI	ACCGGT	1 cut(s)	66
PkrI	GCNGC	2 cut(s)	246, 460
PleI	GAGTC	2 cut(s)	360, 458
PluTI	GGCGCC	1 cut(s)	171
PmeI	GTTTAAAC	1 cut(s)	268
PpsI	GAGTC	2 cut(s)	360, 458
PspN4I	GGNNCC	4 cut(s)	106, 163, 169, 181
PspPI	GGNCC	8 cut(s)	64, 69, 105, 162, 222, 388, 440, 452
PspXI	VCTCGAGB	2 cut(s)	314, 416
PstI	CTGCAG	1 cut(s)	246
PstNI	CAGNNNCTG	1 cut(s)	605
PvuII	CAGCTG	1 cut(s)	458
Rsr2I	CGGWCCG	1 cut(s)	69
RsrII	CGGWCCG	1 cut(s)	69
SapI	GCTCTTC	1 cut(s)	327
SaqAI	TTAA	1 cut(s)	267
SatI	GCNGC	2 cut(s)	245, 459
Sau3AI	GATC	2 cut(s)	209, 238
Sau96I	GGNCC	8 cut(s)	64, 69, 105, 162, 222, 388, 440, 452
SchI	GAGTC	2 cut(s)	361, 458
ScrFI	CCNGG	4 cut(s)	159, 160, 281, 434
SduI	GDGCHC	1 cut(s)	572
SetI	ASST	9 cut(s)	23, 249, 366, 371, 387, 460, 534, 542, 575
SfaNI	GCATC	2 cut(s)	363, 469
SfcI	CTRYAG	1 cut(s)	242
SfoI	GGCGCC	1 cut(s)	169
Sfr274I	CTCGAG	2 cut(s)	314, 416
SgrAI	CRCCGGYG	1 cut(s)	170
SinI	GGWCC	6 cut(s)	64, 69, 105, 222, 388, 452
SlaI	CTCGAG	2 cut(s)	314, 416
SmaI	CCCGGG	1 cut(s)	160
SmlI	CTYRAG	2 cut(s)	314, 416
SmoI	CTYRAG	2 cut(s)	314, 416
Sse9I	AATT	1 cut(s)	15
SsiI	CCGC	7 cut(s)	72, 92, 174, 347, 475, 524, 585
SspDI	GGCGCC	1 cut(s)	167
StyD4I	CCNGG	4 cut(s)	157, 158, 279, 432
TaaI	ACNGT	2 cut(s)	398, 404
TaiI	ACGT	2 cut(s)	371, 575
TaqI	TCGA	5 cut(s)	249, 302, 315, 355, 417
TaqII	GACCGA	1 cut(s)	405
TasI	AATT	1 cut(s)	15
Tru1I	TTAA	1 cut(s)	267
Tru9I	TTAA	1 cut(s)	267
TseFI	GTSAC	1 cut(s)	364
TseI	GCWGC	2 cut(s)	244, 458
Tsp45I	GTSAC	1 cut(s)	364
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	2 cut(s)	515, 525
TspMI	CCCGGG	1 cut(s)	158
VneI	GTGCAC	1 cut(s)	568
VpaK11BI	GGWCC	6 cut(s)	64, 69, 105, 222, 388, 452
XhoI	CTCGAG	2 cut(s)	314, 416
XmaI	CCCGGG	1 cut(s)	158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.