MD14G1069200.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007328
Physical Location & Seq
Reverse (-)
7546561 .. 7547551
991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1069200.v1.1.491

Sequence Viewer

Length: 966 bp
ATGAAGTTGATCAAAATTAGCTCCAAAAATCTCAGTCCGAAATGCCTCTTCGGGTCCAAGGACCGGTCCTCCGTGTCCCGCTGCCCGTCGTTCGGTTCCGGATCGGATCTTTCCTCCTCCTTCTCCTCTTCCCCAGATTCAATTCACAAGCCCGGGGCCGGAGCCGGCGGTATCACCACGCCCACCAGCGTTTTGCCCCAAAAATCGGGTGTCTGGTCCCATTTATCTGCTGATCTCGACGTGGCCCAAGCGTTTAAGCTTATAGACCGGGACAACGACGGAGTGGTGTCGAGGAAAGAACTCGAGGCGTTTCTGTACCAGCTCGGGAACGACCCGCCGAGTCAAGAGGAGGTGACGTTGATGCTGAGCGAGGTGGACCGAGACGGCAACGGTATGATAATCCTTGAGGCTTTATTGAACCAGGTCGGACCGGTTTTCGGTCTAGATGCCGACTCGGAGCTTCGGGATGCGTTTGAAGTGTTTGACACGGATAACGACGGCAAAATATCGGCGGAGGAACTTTTGAGTTTTTCTGCTGATCTCGACGTGGCCCAAGCGTTTAAGCTTATAGACCGGGACAACGACGGAGTGGTGTCGAGGAAAGAACTCGAGGCGTTTCTGTACCAGCTCGGGAACGACCCGCCGAGTCAAGAGGAGGTGACGTTGATGCTGAGCGAGGTGGACCGAGACGGCAACGGTATGATAATCCTTGAGGCTTTATTGAACCAGGTCGTACCGGTTTTCGGTCTAGATGCCGACTCGGAGCTTCGGGATGCGTTTGAAGTGTTTGACACGGATAACGACGGCAAAATATCGGCGGAGGAACTTTTGAGGTTTTTCACGTCTGTCGGAGACGAAGGGTGCACGTTAGAGGAGTGCCGGCGCATGATAGACGGGGTAGATAAGGACGGGGATGGGTTCGTGTGCTTTGAGGAGTTTGTTCATATGATGGAGCCGCAGAGATGA

Protein Analysis

322

Amino Acids

35.03

Weight (kDa)

4.22

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 81 - 133 5.1e-10 EF-hand domain pair
EF-hand_6 PF13405 82 - 109 8.2e-06 EF-hand domain
EF-hand_1 PF00036 153 - 177 1.5e-07 EF hand domain
EF-hand_6 PF13405 153 - 176 4.8e-07 EF-hand domain
EF-hand_5 PF13202 154 - 177 7.3e-08 EF hand
EF-hand_7 PF13499 183 - 235 4.8e-10 EF-hand domain pair
EF-hand_6 PF13405 184 - 211 8.2e-06 EF-hand domain
EF-hand_7 PF13499 254 - 317 2.4e-13 EF-hand domain pair
EF-hand_1 PF00036 255 - 282 2.1e-08 EF hand domain
EF-hand_6 PF13405 255 - 284 9.7e-09 EF-hand domain
EF-hand_5 PF13202 256 - 279 4.3e-08 EF hand
EF-hand_8 PF13833 269 - 318 6.1e-07 EF-hand domain pair
EF-hand_1 PF00036 292 - 318 3.4e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 98
AciI CCGC 7 cut(s) 79, 168, 335, 512, 641, 818, 956
AclWI GGATC 2 cut(s) 109, 114
AfaI GTAC 3 cut(s) 317, 623, 735
AfiI CCNNNNNNNGG 6 cut(s) 63, 92, 158, 205, 437, 743
AgeI ACCGGT 3 cut(s) 63, 430, 736
AgsI TTSAA 5 cut(s) 141, 418, 476, 724, 782
AjiI CACGTC 3 cut(s) 241, 547, 843
AjnI CCWGG 2 cut(s) 420, 726
AluBI AGCT 7 cut(s) 21, 259, 322, 460, 565, 628, 766
AluI AGCT 7 cut(s) 21, 259, 322, 460, 565, 628, 766
Alw21I GWGCWC 1 cut(s) 866
Alw26I GTCTC 3 cut(s) 375, 681, 846
Alw44I GTGCAC 1 cut(s) 862
AlwI GGATC 2 cut(s) 109, 114
Ama87I CYCGRG 5 cut(s) 152, 302, 323, 608, 629
Aor13HI TCCGGA 1 cut(s) 98
AoxI GGCC 3 cut(s) 156, 243, 549
ApaLI GTGCAC 1 cut(s) 862
ApeKI GCWGC 1 cut(s) 81
AsiGI ACCGGT 3 cut(s) 63, 430, 736
AspLEI GCGC 1 cut(s) 885
AsuC2I CCSGG 4 cut(s) 153, 154, 269, 575
AsuHPI GGTGA 3 cut(s) 166, 364, 670
AvaI CYCGRG 5 cut(s) 152, 302, 323, 608, 629
AvaII GGWCC 7 cut(s) 54, 61, 66, 216, 376, 428, 682
BaeGI GKGCMC 1 cut(s) 866
Bbv12I GWGCWC 1 cut(s) 866
BbvI GCAGC 1 cut(s) 68
BccI CCATC 2 cut(s) 908, 943
BceAI ACGGC 4 cut(s) 400, 514, 706, 820
BciT130I CCWGG 2 cut(s) 422, 728
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 4 cut(s) 153, 154, 269, 575
BcoDI GTCTC 3 cut(s) 375, 681, 846
BfaI CTAG 2 cut(s) 443, 749
BisI GCNGC 2 cut(s) 82, 956
BlpI GCTNAGC 2 cut(s) 365, 671
BlsI GCNGC 2 cut(s) 83, 957
Bme1390I CCNGG 6 cut(s) 153, 154, 269, 422, 575, 728
Bme18I GGWCC 7 cut(s) 54, 61, 66, 216, 376, 428, 682
BmeT110I CYCGRG 5 cut(s) 152, 302, 323, 608, 629
BmgBI CACGTC 3 cut(s) 241, 547, 843
BmiI GGNNCC 6 cut(s) 55, 97, 157, 163, 218, 954
BmrFI CCNGG 6 cut(s) 153, 154, 269, 422, 575, 728
BmsI GCATC 6 cut(s) 351, 436, 457, 657, 742, 763
Bpu1102I GCTNAGC 2 cut(s) 365, 671
BpuEI CTTGAG 2 cut(s) 425, 731
BpuMI CCSGG 4 cut(s) 153, 154, 269, 575
BsaJI CCNNGG 3 cut(s) 57, 152, 153
BsaWI WCCGGW 4 cut(s) 63, 98, 430, 736
BsaXI ACNNNNNCTCC 6 cut(s) 53, 83, 341, 371, 647, 677
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 92, 158, 205, 437, 743
Bse118I RCCGGY 5 cut(s) 63, 164, 430, 736, 879
BseAI TCCGGA 1 cut(s) 98
BseBI CCWGG 2 cut(s) 422, 728
BseDI CCNNGG 3 cut(s) 57, 152, 153
BseGI GGATG 3 cut(s) 472, 778, 919
BseLI CCNNNNNNNGG 6 cut(s) 63, 92, 158, 205, 437, 743
BseMII CTCAG 3 cut(s) 46, 356, 662
BseRI GAGGAG 6 cut(s) 106, 115, 362, 668, 887, 947
BseSI GKGCMC 1 cut(s) 866
BseXI GCAGC 1 cut(s) 68
BshFI GGCC 3 cut(s) 158, 245, 551
BshTI ACCGGT 3 cut(s) 63, 430, 736
BsiHKAI GWGCWC 1 cut(s) 866
BsiHKCI CYCGRG 5 cut(s) 152, 302, 323, 608, 629
BslFI GGGAC 4 cut(s) 61, 202, 284, 590
BslI CCNNNNNNNGG 6 cut(s) 63, 92, 158, 205, 437, 743
BsmAI GTCTC 3 cut(s) 375, 681, 846
BsmBI CGTCTC 3 cut(s) 375, 681, 846
BsmFI GGGAC 4 cut(s) 61, 202, 284, 590
BsnI GGCC 3 cut(s) 158, 245, 551
BsoBI CYCGRG 5 cut(s) 152, 302, 323, 608, 629
Bsp1286I GDGCHC 1 cut(s) 866
Bsp13I TCCGGA 1 cut(s) 98
Bsp143I GATC 5 cut(s) 9, 101, 106, 232, 538
Bsp1720I GCTNAGC 2 cut(s) 365, 671
BspACI CCGC 7 cut(s) 79, 168, 335, 512, 641, 818, 956
BspANI GGCC 3 cut(s) 158, 245, 551
BspCNI CTCAG 3 cut(s) 45, 357, 663
BspEI TCCGGA 1 cut(s) 98
BspLI GGNNCC 6 cut(s) 55, 97, 157, 163, 218, 954
BspPI GGATC 2 cut(s) 109, 114
BsrFI RCCGGY 5 cut(s) 63, 164, 430, 736, 879
BssAI RCCGGY 5 cut(s) 63, 164, 430, 736, 879
BssECI CCNNGG 3 cut(s) 57, 152, 153
BssMI GATC 5 cut(s) 9, 101, 106, 232, 538
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 2 cut(s) 422, 728
Bst4CI ACNGT 2 cut(s) 392, 698
Bst6I CTCTTC 2 cut(s) 53, 133
BstC8I GCNNGC 2 cut(s) 166, 881
BstDEI CTNAG 3 cut(s) 32, 365, 671
BstF5I GGATG 3 cut(s) 472, 778, 919
BstHHI GCGC 1 cut(s) 885
BstKTI GATC 5 cut(s) 12, 104, 109, 235, 541
BstMAI GTCTC 3 cut(s) 375, 681, 846
BstMBI GATC 5 cut(s) 9, 101, 106, 232, 538
BstNI CCWGG 2 cut(s) 422, 728
BstSCI CCNGG 6 cut(s) 151, 152, 267, 420, 573, 726
BstSLI GKGCMC 1 cut(s) 866
BstV1I GCAGC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BsuRI GGCC 3 cut(s) 158, 245, 551
BtrI CACGTC 3 cut(s) 241, 547, 843
BtsCI GGATG 3 cut(s) 472, 778, 919
Cac8I GCNNGC 2 cut(s) 166, 881
CfoI GCGC 1 cut(s) 885
Cfr10I RCCGGY 5 cut(s) 63, 164, 430, 736, 879
Cfr9I CCCGGG 1 cut(s) 152
CpoI CGGWCCG 1 cut(s) 428
CsiI ACCWGGT 2 cut(s) 420, 726
Csp6I GTAC 3 cut(s) 316, 622, 734
CspAI ACCGGT 3 cut(s) 63, 430, 736
CspI CGGWCCG 1 cut(s) 428
CviAII CATG 1 cut(s) 886
CviQI GTAC 3 cut(s) 316, 622, 734
DdeI CTNAG 3 cut(s) 32, 365, 671
DpnI GATC 5 cut(s) 11, 103, 108, 234, 540
DpnII GATC 5 cut(s) 9, 101, 106, 232, 538
Eam1104I CTCTTC 2 cut(s) 53, 133
EarI CTCTTC 2 cut(s) 53, 133
EciI GGCGGA 2 cut(s) 527, 833
Eco130I CCWWGG 1 cut(s) 57
Eco47I GGWCC 7 cut(s) 54, 61, 66, 216, 376, 428, 682
Eco88I CYCGRG 5 cut(s) 152, 302, 323, 608, 629
EcoRII CCWGG 2 cut(s) 420, 726
EcoT14I CCWWGG 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 57
Esp3I CGTCTC 3 cut(s) 375, 681, 846
FaeI CATG 1 cut(s) 889
FaiI YATR 7 cut(s) 263, 395, 569, 701, 887, 945, 947
FaqI GGGAC 4 cut(s) 61, 202, 284, 590
FatI CATG 1 cut(s) 885
FauI CCCGC 3 cut(s) 86, 342, 648
FauNDI CATATG 1 cut(s) 945
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 82, 956
FokI GGATG 3 cut(s) 479, 785, 926
Fsp4HI GCNGC 2 cut(s) 82, 956
FspBI CTAG 2 cut(s) 443, 749
GlaI GCGC 1 cut(s) 884
GluI GCNGC 2 cut(s) 82, 956
HaeIII GGCC 3 cut(s) 158, 245, 551
HhaI GCGC 1 cut(s) 885
Hin1II CATG 1 cut(s) 889
Hin6I GCGC 1 cut(s) 883
HinP1I GCGC 1 cut(s) 883
HindIII AAGCTT 2 cut(s) 257, 563
HinfI GANTC 5 cut(s) 137, 340, 452, 646, 758
HphI GGTGA 3 cut(s) 166, 364, 670
Hpy166II GTNNAC 3 cut(s) 376, 682, 864
Hpy188I TCNGA 6 cut(s) 39, 106, 428, 457, 763, 851
Hpy8I GTNNAC 3 cut(s) 376, 682, 864
Hpy99I CGWCG 7 cut(s) 91, 242, 281, 500, 548, 587, 806
HpyAV CCTTC 2 cut(s) 130, 851
HpyCH4III ACNGT 2 cut(s) 392, 698
HpyCH4IV ACGT 6 cut(s) 240, 356, 546, 662, 842, 866
HpyCH4V TGCA 1 cut(s) 864
HpyF3I CTNAG 3 cut(s) 32, 365, 671
HpySE526I ACGT 6 cut(s) 240, 356, 546, 662, 842, 866
Hsp92II CATG 1 cut(s) 889
HspAI GCGC 1 cut(s) 883
Kpn2I TCCGGA 1 cut(s) 98
KroI GCCGGC 2 cut(s) 164, 879
KroNI GCCGGC 2 cut(s) 166, 881
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 5 cut(s) 9, 101, 106, 232, 538
LmnI GCTCC 5 cut(s) 26, 161, 457, 763, 952
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 6 cut(s) 351, 436, 457, 657, 742, 763
MabI ACCWGGT 2 cut(s) 420, 726
MaeI CTAG 2 cut(s) 443, 749
MaeII ACGT 6 cut(s) 240, 356, 546, 662, 842, 866
MaeIII GTNAC 2 cut(s) 352, 658
MalI GATC 5 cut(s) 11, 103, 108, 234, 540
MboI GATC 5 cut(s) 9, 101, 106, 232, 538
MboII GAAGA 2 cut(s) 40, 120
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 866
MluCI AATT 2 cut(s) 15, 141
MlyI GAGTC 4 cut(s) 349, 446, 655, 752
MmeI TCCRAC 2 cut(s) 406, 829
MroI TCCGGA 1 cut(s) 98
MroNI GCCGGC 2 cut(s) 164, 879
MseI TTAA 2 cut(s) 255, 561
MspA1I CMGCKG 1 cut(s) 81
MspR9I CCNGG 6 cut(s) 153, 154, 269, 422, 575, 728
MvaI CCWGG 2 cut(s) 422, 728
NaeI GCCGGC 2 cut(s) 166, 881
NciI CCSGG 4 cut(s) 153, 154, 269, 575
NdeI CATATG 1 cut(s) 945
NdeII GATC 5 cut(s) 9, 101, 106, 232, 538
NgoMIV GCCGGC 2 cut(s) 164, 879
NlaIII CATG 1 cut(s) 889
NlaIV GGNNCC 6 cut(s) 55, 97, 157, 163, 218, 954
NmeAIII GCCGAG 2 cut(s) 363, 669
NmuCI GTSAC 2 cut(s) 352, 658
PaeR7I CTCGAG 2 cut(s) 302, 608
PdiI GCCGGC 2 cut(s) 166, 881
PfeI GAWTC 1 cut(s) 137
PinAI ACCGGT 3 cut(s) 63, 430, 736
PkrI GCNGC 2 cut(s) 83, 957
PleI GAGTC 4 cut(s) 348, 446, 654, 752
PpsI GAGTC 4 cut(s) 348, 446, 654, 752
Psp6I CCWGG 2 cut(s) 420, 726
PspGI CCWGG 2 cut(s) 420, 726
PspN4I GGNNCC 6 cut(s) 55, 97, 157, 163, 218, 954
PspXI VCTCGAGB 2 cut(s) 302, 608
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 3 cut(s) 317, 623, 735
RsaNI GTAC 3 cut(s) 316, 622, 734
Rsr2I CGGWCCG 1 cut(s) 428
RsrII CGGWCCG 1 cut(s) 428
SaqAI TTAA 2 cut(s) 255, 561
SatI GCNGC 2 cut(s) 82, 956
Sau3AI GATC 5 cut(s) 9, 101, 106, 232, 538
SchI GAGTC 4 cut(s) 349, 446, 655, 752
ScrFI CCNGG 6 cut(s) 153, 154, 269, 422, 575, 728
SduI GDGCHC 1 cut(s) 866
SexAI ACCWGGT 2 cut(s) 420, 726
SfaNI GCATC 6 cut(s) 351, 436, 457, 657, 742, 763
Sfr274I CTCGAG 2 cut(s) 302, 608
SinI GGWCC 7 cut(s) 54, 61, 66, 216, 376, 428, 682
SlaI CTCGAG 2 cut(s) 302, 608
SmaI CCCGGG 1 cut(s) 154
SmlI CTYRAG 4 cut(s) 302, 404, 608, 710
SmoI CTYRAG 4 cut(s) 302, 404, 608, 710
Sse9I AATT 2 cut(s) 15, 141
SsiI CCGC 7 cut(s) 79, 168, 335, 512, 641, 818, 956
SspMI CTAG 2 cut(s) 443, 749
StyD4I CCNGG 6 cut(s) 151, 152, 267, 420, 573, 726
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 2 cut(s) 392, 698
TaiI ACGT 6 cut(s) 243, 359, 549, 665, 845, 869
TaqI TCGA 6 cut(s) 237, 290, 303, 543, 596, 609
TaqII GACCGA 4 cut(s) 393, 428, 699, 734
TasI AATT 2 cut(s) 15, 141
TauI GCSGC 1 cut(s) 958
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 2 cut(s) 255, 561
Tru9I TTAA 2 cut(s) 255, 561
TseFI GTSAC 2 cut(s) 352, 658
TseI GCWGC 1 cut(s) 81
Tsp45I GTSAC 2 cut(s) 352, 658
TspDTI ATGAA 2 cut(s) 17, 932
TspGWI ACGGA 5 cut(s) 61, 294, 503, 600, 809
TspMI CCCGGG 1 cut(s) 152
VneI GTGCAC 1 cut(s) 862
VpaK11BI GGWCC 7 cut(s) 54, 61, 66, 216, 376, 428, 682
XbaI TCTAGA 2 cut(s) 442, 748
XhoI CTCGAG 2 cut(s) 302, 608
XmaI CCCGGG 1 cut(s) 152
XspI CTAG 2 cut(s) 443, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.