pycom14g07230

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007328
Physical Location & Seq
Forward (+)
7259731 .. 7260402
672 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07230.1

Sequence Viewer

Length: 672 bp
ATGAAGCTCATCAAAATTAGCCCTAAAAATCTCAGTCCGAAACGCCTCTTCGGGTCCAAGAAGGACCGGTCCTCCGTGTCCCGCTCCGAGCCGCCGTCGTTCGGTTCCGGAGCGGATTCTTCCTCCTCCTCCTCTTCCTCGGACTCAATTCACAAGCCCGGGGCCGGAGCCGGAGCCGGCGGTATCACCACGCCCACCAGCGTTTTGCCGGAAAAATCGGGTGACTGGTCCGATTTTTCGGCCGATCTCGACATGGCCCAAGCGTTTAAGCTTATAGACCGGGACAACGACGGAGTGGTGTCGAGGAAAGAACTCGAGGCGCTTCTGTGCCGGCTCGGGAACGACCCGCCGAGTCAAGAGGAGGTGACGTTGATGCTGAGCGAGGTGGACCGAGACGGCAACGGTTCGATAACCCTTGAGGCTTTATTGAACCAGGTCGGACCGGTTTCCGGTCCAGCTGCCGACTCGGAGCTTCGGGATGCGTTTGAAGTGTTTGACACGGATAACGACGGCAAAATATCGGCGGAGGAACTTTTGAGGGTTTTCACGGCTATAGGGGACGAACGGTGCACGTTAGAGGAGTGCCGGCGTATGATAGACGGGGTAGATAAGGACGGGGATGGGTTCGTGTGCTATGAGGAGTTTGTTCATATGATGGAGCTGCAGAGATGA

Protein Analysis

224

Amino Acids

24.01

Weight (kDa)

4.53

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 84 - 140 2e-11 EF-hand domain pair
EF-hand_6 PF13405 85 - 113 2.6e-06 EF-hand domain
EF-hand_7 PF13499 156 - 219 1.5e-13 EF-hand domain pair
EF-hand_1 PF00036 157 - 184 1.5e-08 EF hand domain
EF-hand_6 PF13405 157 - 186 8.8e-09 EF-hand domain
EF-hand_5 PF13202 158 - 181 2.8e-08 EF hand
EF-hand_1 PF00036 194 - 220 3.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 84, 113
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 6 cut(s) 82, 92, 113, 180, 347, 524
AcoI YGGCCR 1 cut(s) 240
AfiI CCNNNNNNNGG 3 cut(s) 101, 164, 449
AgeI ACCGGT 2 cut(s) 66, 442
AgsI TTSAA 2 cut(s) 430, 488
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 5 cut(s) 7, 271, 458, 472, 661
AluI AGCT 5 cut(s) 7, 271, 458, 472, 661
Alw21I GWGCWC 1 cut(s) 572
Alw26I GTCTC 1 cut(s) 387
Alw44I GTGCAC 1 cut(s) 568
Ama87I CYCGRG 3 cut(s) 158, 314, 335
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 3 cut(s) 162, 240, 255
ApaLI GTGCAC 1 cut(s) 568
ApeKI GCWGC 2 cut(s) 458, 661
AsiGI ACCGGT 2 cut(s) 66, 442
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 9 cut(s) 54, 64, 69, 162, 228, 256, 388, 440, 452
AsuC2I CCSGG 3 cut(s) 159, 160, 281
AsuHPI GGTGA 3 cut(s) 178, 233, 376
AvaI CYCGRG 3 cut(s) 158, 314, 335
AvaII GGWCC 7 cut(s) 54, 64, 69, 228, 388, 440, 452
BaeGI GKGCMC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 572
BbvI GCAGC 2 cut(s) 445, 648
BccI CCATC 2 cut(s) 614, 649
BceAI ACGGC 4 cut(s) 79, 412, 526, 564
BciT130I CCWGG 1 cut(s) 434
BcnI CCSGG 3 cut(s) 159, 160, 281
BcoDI GTCTC 1 cut(s) 387
BfmI CTRYAG 2 cut(s) 552, 662
BfoI RGCGCY 1 cut(s) 323
BisI GCNGC 3 cut(s) 92, 459, 662
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 3 cut(s) 93, 460, 663
Bme1390I CCNGG 4 cut(s) 159, 160, 281, 434
Bme18I GGWCC 7 cut(s) 54, 64, 69, 228, 388, 440, 452
BmeT110I CYCGRG 3 cut(s) 158, 314, 335
BmgT120I GGNCC 9 cut(s) 54, 64, 69, 162, 228, 256, 388, 440, 452
BmiI GGNNCC 5 cut(s) 55, 106, 163, 169, 175
BmrFI CCNGG 4 cut(s) 159, 160, 281, 434
BmsI GCATC 2 cut(s) 363, 469
Bpu1102I GCTNAGC 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 3 cut(s) 159, 160, 281
BsaJI CCNNGG 3 cut(s) 138, 158, 159
BsaWI WCCGGW 4 cut(s) 66, 107, 442, 449
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 353, 383
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 164, 449
Bse118I RCCGGY 5 cut(s) 66, 176, 330, 442, 585
Bse1I ACTGG 1 cut(s) 230
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 3 cut(s) 138, 158, 159
BseGI GGATG 2 cut(s) 484, 625
BseLI CCNNNNNNNGG 3 cut(s) 101, 164, 449
BseMII CTCAG 2 cut(s) 46, 368
BseNI ACTGG 1 cut(s) 230
BseRI GAGGAG 6 cut(s) 115, 118, 121, 374, 593, 653
BseSI GKGCMC 1 cut(s) 572
BseX3I CGGCCG 1 cut(s) 240
BseXI GCAGC 2 cut(s) 445, 648
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 3 cut(s) 164, 242, 257
BshTI ACCGGT 2 cut(s) 66, 442
BsiEI CGRYCG 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 572
BsiHKCI CYCGRG 3 cut(s) 158, 314, 335
BslFI GGGAC 3 cut(s) 64, 296, 572
BslI CCNNNNNNNGG 3 cut(s) 101, 164, 449
BsmAI GTCTC 1 cut(s) 387
BsmBI CGTCTC 1 cut(s) 387
BsmFI GGGAC 3 cut(s) 64, 296, 572
BsnI GGCC 3 cut(s) 164, 242, 257
BsoBI CYCGRG 3 cut(s) 158, 314, 335
Bsp1286I GDGCHC 1 cut(s) 572
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 1 cut(s) 244
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 6 cut(s) 82, 92, 113, 180, 347, 524
BspANI GGCC 3 cut(s) 164, 242, 257
BspCNI CTCAG 2 cut(s) 45, 369
BspEI TCCGGA 1 cut(s) 107
BspLI GGNNCC 5 cut(s) 55, 106, 163, 169, 175
BspMAI CTGCAG 1 cut(s) 666
BsrBI CCGCTC 2 cut(s) 84, 113
BsrFI RCCGGY 5 cut(s) 66, 176, 330, 442, 585
BsrI ACTGG 1 cut(s) 230
BssAI RCCGGY 5 cut(s) 66, 176, 330, 442, 585
BssECI CCNNGG 3 cut(s) 138, 158, 159
BssMI GATC 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 2 cut(s) 404, 567
Bst6I CTCTTC 2 cut(s) 53, 139
BstC8I GCNNGC 3 cut(s) 178, 332, 587
BstDEI CTNAG 2 cut(s) 32, 377
BstF5I GGATG 2 cut(s) 484, 625
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 1 cut(s) 244
BstMCI CGRYCG 1 cut(s) 243
BstNI CCWGG 1 cut(s) 434
BstSCI CCNGG 4 cut(s) 157, 158, 279, 432
BstSFI CTRYAG 2 cut(s) 552, 662
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 2 cut(s) 445, 648
BstZI CGGCCG 1 cut(s) 240
BsuRI GGCC 3 cut(s) 164, 242, 257
BtsCI GGATG 2 cut(s) 484, 625
Cac8I GCNNGC 3 cut(s) 178, 332, 587
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 5 cut(s) 66, 176, 330, 442, 585
Cfr13I GGNCC 9 cut(s) 54, 64, 69, 162, 228, 256, 388, 440, 452
Cfr9I CCCGGG 1 cut(s) 158
CpoI CGGWCCG 1 cut(s) 440
CsiI ACCWGGT 1 cut(s) 432
CspAI ACCGGT 2 cut(s) 66, 442
CspI CGGWCCG 1 cut(s) 440
CviAII CATG 1 cut(s) 253
DdeI CTNAG 2 cut(s) 32, 377
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 240
EagI CGGCCG 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 53, 139
EarI CTCTTC 2 cut(s) 53, 139
EciI GGCGGA 1 cut(s) 539
EclXI CGGCCG 1 cut(s) 240
Eco47I GGWCC 7 cut(s) 54, 64, 69, 228, 388, 440, 452
Eco52I CGGCCG 1 cut(s) 240
Eco88I CYCGRG 3 cut(s) 158, 314, 335
EcoRII CCWGG 1 cut(s) 432
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 1 cut(s) 256
FaiI YATR 7 cut(s) 254, 275, 554, 593, 636, 651, 653
FaqI GGGAC 3 cut(s) 64, 296, 572
FatI CATG 1 cut(s) 252
FauI CCCGC 2 cut(s) 89, 354
FauNDI CATATG 1 cut(s) 651
Fnu4HI GCNGC 3 cut(s) 92, 459, 662
FokI GGATG 2 cut(s) 491, 632
Fsp4HI GCNGC 3 cut(s) 92, 459, 662
GlaI GCGC 1 cut(s) 321
GluI GCNGC 3 cut(s) 92, 459, 662
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 3 cut(s) 164, 242, 257
HhaI GCGC 1 cut(s) 322
Hin1II CATG 1 cut(s) 256
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 4 cut(s) 116, 143, 352, 464
HphI GGTGA 3 cut(s) 178, 233, 376
Hpy166II GTNNAC 2 cut(s) 388, 570
Hpy188I TCNGA 6 cut(s) 39, 88, 142, 232, 440, 469
Hpy188III TCNNGA 5 cut(s) 108, 248, 337, 356, 476
Hpy8I GTNNAC 2 cut(s) 388, 570
Hpy99I CGWCG 3 cut(s) 100, 293, 512
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 2 cut(s) 404, 567
HpyCH4IV ACGT 2 cut(s) 368, 572
HpyCH4V TGCA 2 cut(s) 570, 664
HpyF3I CTNAG 2 cut(s) 32, 377
HpySE526I ACGT 2 cut(s) 368, 572
Hsp92II CATG 1 cut(s) 256
HspAI GCGC 1 cut(s) 320
Kpn2I TCCGGA 1 cut(s) 107
KroI GCCGGC 3 cut(s) 176, 330, 585
KroNI GCCGGC 3 cut(s) 178, 332, 587
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 6 cut(s) 89, 110, 167, 173, 469, 658
Lsp1109I GCAGC 2 cut(s) 445, 648
LweI GCATC 2 cut(s) 363, 469
MabI ACCWGGT 1 cut(s) 432
MaeII ACGT 2 cut(s) 368, 572
MaeIII GTNAC 2 cut(s) 221, 364
MalI GATC 1 cut(s) 246
MbiI CCGCTC 2 cut(s) 84, 113
MboI GATC 1 cut(s) 244
MboII GAAGA 3 cut(s) 40, 111, 126
MhlI GDGCHC 1 cut(s) 572
MluCI AATT 2 cut(s) 15, 147
MlyI GAGTC 3 cut(s) 137, 361, 458
MmeI TCCRAC 1 cut(s) 418
MroI TCCGGA 1 cut(s) 107
MroNI GCCGGC 3 cut(s) 176, 330, 585
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 458
MspR9I CCNGG 4 cut(s) 159, 160, 281, 434
MvaI CCWGG 1 cut(s) 434
NaeI GCCGGC 3 cut(s) 178, 332, 587
NciI CCSGG 3 cut(s) 159, 160, 281
NdeI CATATG 1 cut(s) 651
NdeII GATC 1 cut(s) 244
NgoMIV GCCGGC 3 cut(s) 176, 330, 585
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 5 cut(s) 55, 106, 163, 169, 175
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 2 cut(s) 221, 364
PaeR7I CTCGAG 1 cut(s) 314
PdiI GCCGGC 3 cut(s) 178, 332, 587
PfeI GAWTC 1 cut(s) 116
PinAI ACCGGT 2 cut(s) 66, 442
PkrI GCNGC 3 cut(s) 93, 460, 663
PleI GAGTC 3 cut(s) 137, 360, 458
PpsI GAGTC 3 cut(s) 137, 360, 458
Psp6I CCWGG 1 cut(s) 432
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 5 cut(s) 55, 106, 163, 169, 175
PspPI GGNCC 9 cut(s) 54, 64, 69, 162, 228, 256, 388, 440, 452
PspXI VCTCGAGB 1 cut(s) 314
PstI CTGCAG 1 cut(s) 666
PvuII CAGCTG 1 cut(s) 458
Rsr2I CGGWCCG 1 cut(s) 440
RsrII CGGWCCG 1 cut(s) 440
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 3 cut(s) 92, 459, 662
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 9 cut(s) 54, 64, 69, 162, 228, 256, 388, 440, 452
SchI GAGTC 3 cut(s) 137, 361, 458
ScrFI CCNGG 4 cut(s) 159, 160, 281, 434
SduI GDGCHC 1 cut(s) 572
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 2 cut(s) 363, 469
SfcI CTRYAG 2 cut(s) 552, 662
Sfr274I CTCGAG 1 cut(s) 314
SinI GGWCC 7 cut(s) 54, 64, 69, 228, 388, 440, 452
SlaI CTCGAG 1 cut(s) 314
SmaI CCCGGG 1 cut(s) 160
SmlI CTYRAG 2 cut(s) 314, 416
SmoI CTYRAG 2 cut(s) 314, 416
Sse9I AATT 2 cut(s) 15, 147
SsiI CCGC 6 cut(s) 82, 92, 113, 180, 347, 524
StyD4I CCNGG 4 cut(s) 157, 158, 279, 432
TaaI ACNGT 2 cut(s) 404, 567
TaiI ACGT 2 cut(s) 371, 575
TaqI TCGA 4 cut(s) 249, 302, 315, 407
TaqII GACCGA 1 cut(s) 405
TasI AATT 2 cut(s) 15, 147
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TseFI GTSAC 2 cut(s) 221, 364
TseI GCWGC 2 cut(s) 458, 661
Tsp45I GTSAC 2 cut(s) 221, 364
TspDTI ATGAA 2 cut(s) 17, 638
TspGWI ACGGA 3 cut(s) 64, 306, 515
TspMI CCCGGG 1 cut(s) 158
VneI GTGCAC 1 cut(s) 568
VpaK11BI GGWCC 7 cut(s) 54, 64, 69, 228, 388, 440, 452
XhoI CTCGAG 1 cut(s) 314
XmaI CCCGGG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.