Rh2BG320600

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007328
Physical Location & Seq
Forward (+)
38355954 .. 38357619
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG320600.1

Sequence Viewer

Length: 660 bp
ATGAAGCTCATGAAAATCAACAGCCTCAGCCCCAAACGCCTCTTCCGCTCCATGAAGGACCGCTCCGCTGTTTCCCGCTCCGACCCACCGTCCTTTGGCTCCGGAACGGCGTCGTCTTCCTCCTCGGATCCCCTGCCGTCCGACCACAAGCCCGGACCCGGCGGCATGGTGACTCCCACGAGCGTTCTGCCGGAAATATCCGGGGACTGGTCGGATTTCTCTGCCGACACTCATCTCGACATGGCCCAGGCGTTTAAGCTGATCGACAGAGACAACGACGGAGTGGTGTCGAGAAAAGAGCTGGAGGCTCTGCTGAAGCGCCTGGGGGCGGAGCCGCCGAGCCACGAAGAGGTGGCGATGATGTTGAGCGAGGTGGACCGGGACGGCGACGGCTGCATACCCCTGGAGGCCTTGTTGCACCGGTTCGGACCGGCTTGCGAACCGGCGGCCGACTCGGAGCTGCGGGAGGCTTTTGAGGTGTTCGACGCGGATCACGACGGCAAAATATCGGCGGAGGAGCTGTTCAGCGTGTTCACGGCGATAGGGGACGACCGGTGCACGTTAGAGGACTGCCGGCGCATGATAGCCGAGGTCGATAAGAACGGGGATGGGTTCGTCTGCTTTGAGGACTTTGCTCTTATGATGGAGCTGCAGAGATGA

Protein Analysis

219

Amino Acids

23.97

Weight (kDa)

4.58

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 81 - 136 1.2e-11 EF-hand domain pair
EF-hand_6 PF13405 81 - 109 5.8e-07 EF-hand domain
EF-hand_1 PF00036 82 - 108 5.6e-06 EF hand domain
EF-hand_7 PF13499 152 - 215 1e-12 EF-hand domain pair
EF-hand_1 PF00036 153 - 180 6.6e-08 EF hand domain
EF-hand_6 PF13405 153 - 182 9.5e-08 EF-hand domain
EF-hand_5 PF13202 154 - 174 9e-07 EF hand
EF-hand_1 PF00036 190 - 216 2.4e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 48, 63, 78
AccII CGCG 1 cut(s) 488
AccIII TCCGGA 1 cut(s) 101
AclWI GGATC 3 cut(s) 122, 135, 498
AcoI YGGCCR 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 110
AfiI CCNNNNNNNGG 5 cut(s) 95, 158, 207, 328, 349
AgeI ACCGGT 2 cut(s) 420, 552
AhdI GACNNNNNGTC 1 cut(s) 88
AjnI CCWGG 3 cut(s) 246, 321, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 6 cut(s) 7, 259, 301, 460, 520, 649
AluI AGCT 6 cut(s) 7, 259, 301, 460, 520, 649
Alw21I GWGCWC 1 cut(s) 560
Alw26I GTCTC 1 cut(s) 264
Alw44I GTGCAC 1 cut(s) 556
AlwI GGATC 3 cut(s) 122, 135, 498
Aor13HI TCCGGA 1 cut(s) 101
AoxI GGCC 3 cut(s) 243, 408, 447
ApaLI GTGCAC 1 cut(s) 556
ApeKI GCWGC 3 cut(s) 393, 460, 649
AsiGI ACCGGT 2 cut(s) 420, 552
AspLEI GCGC 2 cut(s) 321, 579
AspS9I GGNCC 5 cut(s) 58, 155, 244, 376, 428
AsuC2I CCSGG 4 cut(s) 153, 159, 202, 380
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 4 cut(s) 58, 155, 376, 428
BaeGI GKGCMC 1 cut(s) 560
BamHI GGATCC 1 cut(s) 127
BauI CACGAG 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 560
BbvCI CCTCAGC 1 cut(s) 26
BbvI GCAGC 3 cut(s) 380, 447, 636
BccI CCATC 2 cut(s) 602, 637
BceAI ACGGC 6 cut(s) 121, 123, 400, 406, 514, 552
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BciT130I CCWGG 3 cut(s) 248, 323, 404
BcnI CCSGG 4 cut(s) 153, 159, 202, 380
BcoDI GTCTC 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 650
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 6 cut(s) 163, 335, 394, 447, 461, 650
BlsI GCNGC 6 cut(s) 164, 336, 395, 448, 462, 651
Bme1390I CCNGG 7 cut(s) 153, 159, 202, 248, 323, 380, 404
Bme18I GGWCC 4 cut(s) 58, 155, 376, 428
BmeRI GACNNNNNGTC 1 cut(s) 88
BmgT120I GGNCC 5 cut(s) 58, 155, 244, 376, 428
BmiI GGNNCC 4 cut(s) 100, 129, 157, 333
BmrFI CCNGG 7 cut(s) 153, 159, 202, 248, 323, 380, 404
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 2 cut(s) 323, 425
Bpu10I CCTNAGC 1 cut(s) 26
BpuMI CCSGG 4 cut(s) 153, 159, 202, 380
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 6 cut(s) 123, 201, 246, 322, 402, 588
BsaWI WCCGGW 3 cut(s) 101, 420, 552
BsaXI ACNNNNNCTCC 4 cut(s) 398, 428, 506, 536
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 158, 207, 328, 349
Bse118I RCCGGY 5 cut(s) 420, 430, 442, 552, 573
Bse1I ACTGG 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 101
BseBI CCWGG 3 cut(s) 248, 323, 404
BseDI CCNNGG 6 cut(s) 123, 201, 246, 322, 402, 588
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 5 cut(s) 95, 158, 207, 328, 349
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 2 cut(s) 112, 530
BseSI GKGCMC 1 cut(s) 560
BseX3I CGGCCG 1 cut(s) 447
BseXI GCAGC 3 cut(s) 380, 447, 636
Bsh1236I CGCG 1 cut(s) 488
Bsh1285I CGRYCG 2 cut(s) 450, 553
BshFI GGCC 3 cut(s) 245, 410, 449
BshTI ACCGGT 2 cut(s) 420, 552
BsiEI CGRYCG 2 cut(s) 450, 553
BsiHKAI GWGCWC 1 cut(s) 560
BslFI GGGAC 3 cut(s) 218, 395, 560
BslI CCNNNNNNNGG 5 cut(s) 95, 158, 207, 328, 349
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 3 cut(s) 218, 395, 560
BsnI GGCC 3 cut(s) 245, 410, 449
Bsp1286I GDGCHC 1 cut(s) 560
Bsp13I TCCGGA 1 cut(s) 101
Bsp143I GATC 3 cut(s) 127, 261, 490
BspANI GGCC 3 cut(s) 245, 410, 449
BspCNI CTCAG 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 101
BspFNI CGCG 1 cut(s) 488
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 4 cut(s) 100, 129, 157, 333
BspMAI CTGCAG 1 cut(s) 654
BspPI GGATC 3 cut(s) 122, 135, 498
BsrBI CCGCTC 3 cut(s) 48, 63, 78
BsrFI RCCGGY 5 cut(s) 420, 430, 442, 552, 573
BsrI ACTGG 1 cut(s) 212
BssAI RCCGGY 5 cut(s) 420, 430, 442, 552, 573
BssECI CCNNGG 6 cut(s) 123, 201, 246, 322, 402, 588
BssMI GATC 3 cut(s) 127, 261, 490
BssNI GRCGYC 1 cut(s) 110
BssSI CACGAG 1 cut(s) 178
Bst2BI CACGAG 1 cut(s) 178
Bst2UI CCWGG 3 cut(s) 248, 323, 404
Bst4CI ACNGT 1 cut(s) 90
Bst6I CTCTTC 2 cut(s) 47, 342
BstACI GRCGYC 1 cut(s) 110
BstC8I GCNNGC 2 cut(s) 436, 575
BstDEI CTNAG 1 cut(s) 26
BstF5I GGATG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 488
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 321, 579
BstKTI GATC 3 cut(s) 130, 264, 493
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 3 cut(s) 127, 261, 490
BstMCI CGRYCG 2 cut(s) 450, 553
BstMWI GCNNNNNNNGC 3 cut(s) 36, 45, 393
BstNI CCWGG 3 cut(s) 248, 323, 404
BstSCI CCNGG 7 cut(s) 151, 157, 200, 246, 321, 378, 402
BstSFI CTRYAG 1 cut(s) 650
BstSLI GKGCMC 1 cut(s) 560
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 3 cut(s) 380, 447, 636
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BstZI CGGCCG 1 cut(s) 447
BsuRI GGCC 3 cut(s) 245, 410, 449
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 1 cut(s) 613
Cac8I GCNNGC 2 cut(s) 436, 575
CciI TCATGA 1 cut(s) 9
CfoI GCGC 2 cut(s) 321, 579
Cfr10I RCCGGY 5 cut(s) 420, 430, 442, 552, 573
Cfr13I GGNCC 5 cut(s) 58, 155, 244, 376, 428
CpoI CGGWCCG 1 cut(s) 428
CseI GACGC 2 cut(s) 99, 494
CspAI ACCGGT 2 cut(s) 420, 552
CspI CGGWCCG 1 cut(s) 428
CviAII CATG 5 cut(s) 10, 52, 166, 241, 580
DdeI CTNAG 1 cut(s) 26
DpnI GATC 3 cut(s) 129, 263, 492
DpnII GATC 3 cut(s) 127, 261, 490
DriI GACNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 447
EagI CGGCCG 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 47, 342
Eam1105I GACNNNNNGTC 1 cut(s) 88
EarI CTCTTC 2 cut(s) 47, 342
EciI GGCGGA 2 cut(s) 344, 527
EclXI CGGCCG 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 410
Eco47I GGWCC 4 cut(s) 58, 155, 376, 428
Eco52I CGGCCG 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 335
EcoRII CCWGG 3 cut(s) 246, 321, 402
FaeI CATG 5 cut(s) 13, 55, 169, 244, 583
FaiI YATR 7 cut(s) 11, 53, 167, 242, 398, 581, 641
FaqI GGGAC 3 cut(s) 218, 395, 560
FatI CATG 5 cut(s) 9, 51, 165, 240, 579
FauI CCCGC 2 cut(s) 83, 456
Fnu4HI GCNGC 6 cut(s) 163, 335, 394, 447, 461, 650
FokI GGATG 1 cut(s) 620
Fsp4HI GCNGC 6 cut(s) 163, 335, 394, 447, 461, 650
GlaI GCGC 2 cut(s) 320, 578
GluI GCNGC 6 cut(s) 163, 335, 394, 447, 461, 650
GsuI CTGGAG 2 cut(s) 323, 425
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 3 cut(s) 245, 410, 449
HgaI GACGC 2 cut(s) 99, 494
HhaI GCGC 2 cut(s) 321, 579
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 5 cut(s) 13, 55, 169, 244, 583
Hin6I GCGC 2 cut(s) 319, 577
HinP1I GCGC 2 cut(s) 319, 577
HinfI GANTC 2 cut(s) 172, 452
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 3 cut(s) 376, 534, 558
Hpy188I TCNGA 6 cut(s) 82, 127, 142, 214, 428, 457
Hpy188III TCNNGA 5 cut(s) 10, 102, 236, 291, 494
Hpy8I GTNNAC 3 cut(s) 376, 534, 558
Hpy99I CGWCG 5 cut(s) 115, 281, 392, 488, 500
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 90
HpyCH4IV ACGT 1 cut(s) 560
HpyCH4V TGCA 4 cut(s) 396, 418, 558, 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 45, 393
HpyF3I CTNAG 1 cut(s) 26
HpySE526I ACGT 1 cut(s) 560
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 5 cut(s) 13, 55, 169, 244, 583
HspAI GCGC 2 cut(s) 319, 577
Kpn2I TCCGGA 1 cut(s) 101
KroI GCCGGC 1 cut(s) 573
KroNI GCCGGC 1 cut(s) 575
Kzo9I GATC 3 cut(s) 127, 261, 490
LmnI GCTCC 8 cut(s) 53, 68, 83, 104, 331, 457, 517, 646
Lsp1109I GCAGC 3 cut(s) 380, 447, 636
MaeII ACGT 1 cut(s) 560
MaeIII GTNAC 1 cut(s) 169
MalI GATC 3 cut(s) 129, 263, 492
MbiI CCGCTC 3 cut(s) 48, 63, 78
MboI GATC 3 cut(s) 127, 261, 490
MboII GAAGA 3 cut(s) 34, 108, 359
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 1 cut(s) 560
MlyI GAGTC 2 cut(s) 166, 446
MmeI TCCRAC 3 cut(s) 105, 165, 192
MroI TCCGGA 1 cut(s) 101
MroNI GCCGGC 1 cut(s) 573
MseI TTAA 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 68
MspR9I CCNGG 7 cut(s) 153, 159, 202, 248, 323, 380, 404
MvaI CCWGG 3 cut(s) 248, 323, 404
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 3 cut(s) 36, 45, 393
NaeI GCCGGC 1 cut(s) 575
NciI CCSGG 4 cut(s) 153, 159, 202, 380
NdeII GATC 3 cut(s) 127, 261, 490
NgoMIV GCCGGC 1 cut(s) 573
NlaIII CATG 5 cut(s) 13, 55, 169, 244, 583
NlaIV GGNNCC 4 cut(s) 100, 129, 157, 333
NmeAIII GCCGAG 2 cut(s) 363, 613
NmuCI GTSAC 1 cut(s) 169
PagI TCATGA 1 cut(s) 9
PceI AGGCCT 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PdiI GCCGGC 1 cut(s) 575
PinAI ACCGGT 2 cut(s) 420, 552
PkrI GCNGC 6 cut(s) 164, 336, 395, 448, 462, 651
PleI GAGTC 2 cut(s) 166, 446
PpsI GAGTC 2 cut(s) 166, 446
Psp6I CCWGG 3 cut(s) 246, 321, 402
PspGI CCWGG 3 cut(s) 246, 321, 402
PspN4I GGNNCC 4 cut(s) 100, 129, 157, 333
PspPI GGNCC 5 cut(s) 58, 155, 244, 376, 428
PstI CTGCAG 1 cut(s) 654
PsuI RGATCY 1 cut(s) 127
Rsr2I CGGWCCG 1 cut(s) 428
RsrII CGGWCCG 1 cut(s) 428
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 6 cut(s) 163, 335, 394, 447, 461, 650
Sau3AI GATC 3 cut(s) 127, 261, 490
Sau96I GGNCC 5 cut(s) 58, 155, 244, 376, 428
SchI GAGTC 2 cut(s) 166, 446
ScrFI CCNGG 7 cut(s) 153, 159, 202, 248, 323, 380, 404
SduI GDGCHC 1 cut(s) 560
SfcI CTRYAG 1 cut(s) 650
SinI GGWCC 4 cut(s) 58, 155, 376, 428
SseBI AGGCCT 1 cut(s) 410
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 7 cut(s) 151, 157, 200, 246, 321, 378, 402
TaaI ACNGT 1 cut(s) 90
TaiI ACGT 1 cut(s) 563
TaqI TCGA 5 cut(s) 237, 264, 290, 483, 594
TauI GCSGC 3 cut(s) 165, 337, 449
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 3 cut(s) 393, 460, 649
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 3 cut(s) 17, 26, 68
TspGWI ACGGA 1 cut(s) 294
VneI GTGCAC 1 cut(s) 556
VpaK11BI GGWCC 4 cut(s) 58, 155, 376, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.