FvH4_6g25060

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007328
Physical Location & Seq
Forward (+)
18969165 .. 18970544
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g25060.t1

Sequence Viewer

Length: 633 bp
ATGAAGCTTATGAAAATCAACAGCCTCAGTCCCAAGCGCCTCTTCCGCTCCAAGAAGGACCGCTCCGCCGTCTCCCGCTCCGACCCTCCCTCCTTCGGCTCCGGAACCGCGTCCTCTTCCTCCTCCGATTCCCTCCACAAGCCTGGTGGTCCTGCCGCCACTCCGACCAGCGTCCTGCCGGGGGACTGGTCGGAGTTCTCTTCTGTCGACATGGCGCAGGCCTTTAGGCTGATCGACAGAGACAACGACGGAGTGGTCTCGAGAACAGAGCTGGAGGCTCTGCTGAAGCGGCTGCTGGGGGCGGAGCCGCCGACCCAGGAGGAGGTGGCGATGATGCTGAGCGAGGTCGACGGCGACGGCGACGGCTGCATACCCCTGGAGGCTCTTTTACACCGGTTCGGACCGGGTTGCGAGCCGGCGGCCGACTCGGAGCTGCGGGAGGCGTTTGAGGTGTTCGACACGGATCACGACGGGAAGATATCCGCGGAGGAGCTGTTGAGCGTGTTCACGGCGATAGGGGACGAACGGTGCACGTTAGAGGAGTGCCGGCGCATGATTGCGGAGGTCGATAAGAACGGGGACGGCTTCGTCTGCTTTCAGGACTTTGCTCGTATGATGGACCTGCAGCGATGA

Protein Analysis

211

Amino Acids

22.91

Weight (kDa)

4.65

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 70 - 127 7.2e-11 EF-hand domain pair
EF-hand_6 PF13405 71 - 97 3e-06 EF-hand domain
EF-hand_1 PF00036 72 - 98 9.8e-06 EF hand domain
EF-hand_7 PF13499 143 - 206 1.6e-14 EF-hand domain pair
EF-hand_1 PF00036 144 - 171 1.3e-08 EF hand domain
EF-hand_6 PF13405 144 - 173 1.3e-08 EF-hand domain
EF-hand_5 PF13202 145 - 167 1.6e-07 EF hand
EF-hand_1 PF00036 181 - 206 8.8e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 254, 587
AccBSI CCGCTC 3 cut(s) 48, 63, 78
AccI GTMKAC 2 cut(s) 207, 348
AccII CGCG 2 cut(s) 110, 485
AccIII TCCGGA 1 cut(s) 101
AclWI GGATC 1 cut(s) 471
AcoI YGGCCR 1 cut(s) 420
AcuI CTGAAG 1 cut(s) 305
AfiI CCNNNNNNNGG 3 cut(s) 95, 181, 322
AgeI ACCGGT 1 cut(s) 393
AjnI CCWGG 3 cut(s) 142, 315, 375
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AluBI AGCT 4 cut(s) 7, 271, 433, 493
AluI AGCT 4 cut(s) 7, 271, 433, 493
Alw21I GWGCWC 1 cut(s) 533
Alw26I GTCTC 3 cut(s) 76, 234, 262
Alw44I GTGCAC 1 cut(s) 529
AlwI GGATC 1 cut(s) 471
Ama87I CYCGRG 1 cut(s) 259
Aor13HI TCCGGA 1 cut(s) 101
AoxI GGCC 2 cut(s) 219, 420
ApaLI GTGCAC 1 cut(s) 529
ApeKI GCWGC 4 cut(s) 292, 366, 433, 625
AsiGI ACCGGT 1 cut(s) 393
AspLEI GCGC 3 cut(s) 39, 217, 552
AspS9I GGNCC 4 cut(s) 58, 149, 401, 619
AsuC2I CCSGG 2 cut(s) 180, 405
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 4 cut(s) 58, 149, 401, 619
BaeGI GKGCMC 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 533
BbvI GCAGC 3 cut(s) 279, 353, 420
BccI CCATC 1 cut(s) 610
BceAI ACGGC 6 cut(s) 53, 367, 373, 379, 525, 598
BciT130I CCWGG 3 cut(s) 144, 317, 377
BcnI CCSGG 2 cut(s) 180, 405
BcoDI GTCTC 3 cut(s) 76, 234, 262
BfmI CTRYAG 1 cut(s) 623
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 8 cut(s) 156, 290, 293, 308, 367, 420, 434, 626
BlpI GCTNAGC 1 cut(s) 338
BlsI GCNGC 8 cut(s) 157, 291, 294, 309, 368, 421, 435, 627
Bme1390I CCNGG 5 cut(s) 144, 180, 317, 377, 405
Bme18I GGWCC 4 cut(s) 58, 149, 401, 619
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 4 cut(s) 58, 149, 401, 619
BmiI GGNNCC 3 cut(s) 100, 106, 306
BmrFI CCNGG 5 cut(s) 144, 180, 317, 377, 405
BmsI GCATC 1 cut(s) 324
BoxI GACNNNNGTC 1 cut(s) 170
BpmI CTGGAG 2 cut(s) 293, 398
Bpu1102I GCTNAGC 1 cut(s) 338
BpuMI CCSGG 2 cut(s) 180, 405
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 4 cut(s) 179, 315, 375, 483
BsaWI WCCGGW 2 cut(s) 101, 393
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 479, 509
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 181, 322
Bse118I RCCGGY 3 cut(s) 393, 415, 546
Bse1I ACTGG 1 cut(s) 191
BseAI TCCGGA 1 cut(s) 101
BseBI CCWGG 3 cut(s) 144, 317, 377
BseDI CCNNGG 4 cut(s) 179, 315, 375, 483
BseLI CCNNNNNNNGG 3 cut(s) 95, 181, 322
BseMII CTCAG 2 cut(s) 40, 329
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 4 cut(s) 112, 335, 503, 554
BseSI GKGCMC 1 cut(s) 533
BseX3I CGGCCG 1 cut(s) 420
BseXI GCAGC 3 cut(s) 279, 353, 420
BseYI CCCAGC 1 cut(s) 295
Bsh1236I CGCG 2 cut(s) 110, 485
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 2 cut(s) 221, 422
BshTI ACCGGT 1 cut(s) 393
BsiEI CGRYCG 1 cut(s) 423
BsiHKAI GWGCWC 1 cut(s) 533
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 6 cut(s) 102, 179, 394, 404, 416, 547
BslFI GGGAC 4 cut(s) 15, 197, 533, 593
BslI CCNNNNNNNGG 3 cut(s) 95, 181, 322
BsmAI GTCTC 3 cut(s) 76, 234, 262
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 4 cut(s) 15, 197, 533, 593
BsnI GGCC 2 cut(s) 221, 422
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 533
Bsp13I TCCGGA 1 cut(s) 101
Bsp143I GATC 2 cut(s) 231, 463
Bsp1720I GCTNAGC 1 cut(s) 338
BspANI GGCC 2 cut(s) 221, 422
BspCNI CTCAG 2 cut(s) 39, 330
BspEI TCCGGA 1 cut(s) 101
BspFNI CGCG 2 cut(s) 110, 485
BspLI GGNNCC 3 cut(s) 100, 106, 306
BspMAI CTGCAG 1 cut(s) 627
BspPI GGATC 1 cut(s) 471
BspTNI GGTCTC 1 cut(s) 262
BsrBI CCGCTC 3 cut(s) 48, 63, 78
BsrFI RCCGGY 3 cut(s) 393, 415, 546
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 3 cut(s) 393, 415, 546
BssECI CCNNGG 4 cut(s) 179, 315, 375, 483
BssMI GATC 2 cut(s) 231, 463
Bst2UI CCWGG 3 cut(s) 144, 317, 377
Bst4CI ACNGT 1 cut(s) 528
Bst6I CTCTTC 3 cut(s) 47, 121, 205
BstC8I GCNNGC 4 cut(s) 219, 413, 417, 548
BstDEI CTNAG 2 cut(s) 26, 338
BstDSI CCRYGG 1 cut(s) 483
BstFNI CGCG 2 cut(s) 110, 485
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 3 cut(s) 39, 217, 552
BstKTI GATC 2 cut(s) 234, 466
BstMAI GTCTC 3 cut(s) 76, 234, 262
BstMBI GATC 2 cut(s) 231, 463
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 4 cut(s) 45, 289, 366, 591
BstNI CCWGG 3 cut(s) 144, 317, 377
BstPAI GACNNNNGTC 1 cut(s) 170
BstSCI CCNGG 5 cut(s) 142, 178, 315, 375, 403
BstSFI CTRYAG 1 cut(s) 623
BstSLI GKGCMC 1 cut(s) 533
BstUI CGCG 2 cut(s) 110, 485
BstV1I GCAGC 3 cut(s) 279, 353, 420
BstXI CCANNNNNNTGG 1 cut(s) 143
BstZI CGGCCG 1 cut(s) 420
BsuRI GGCC 2 cut(s) 221, 422
BtgI CCRYGG 1 cut(s) 483
BtgZI GCGATG 1 cut(s) 344
Cac8I GCNNGC 4 cut(s) 219, 413, 417, 548
CfoI GCGC 3 cut(s) 39, 217, 552
Cfr10I RCCGGY 3 cut(s) 393, 415, 546
Cfr13I GGNCC 4 cut(s) 58, 149, 401, 619
Cfr42I CCGCGG 1 cut(s) 486
CpoI CGGWCCG 1 cut(s) 401
CseI GACGC 2 cut(s) 99, 160
CspAI ACCGGT 1 cut(s) 393
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CspI CGGWCCG 1 cut(s) 401
CviAII CATG 2 cut(s) 211, 553
DdeI CTNAG 2 cut(s) 26, 338
DpnI GATC 2 cut(s) 233, 465
DpnII GATC 2 cut(s) 231, 463
DrdI GACNNNNNNGTC 2 cut(s) 254, 587
DseDI GACNNNNNNGTC 2 cut(s) 254, 587
EaeI YGGCCR 1 cut(s) 420
EagI CGGCCG 1 cut(s) 420
Eam1104I CTCTTC 3 cut(s) 47, 121, 205
EarI CTCTTC 3 cut(s) 47, 121, 205
EciI GGCGGA 2 cut(s) 55, 317
EclXI CGGCCG 1 cut(s) 420
Eco147I AGGCCT 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 262
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 4 cut(s) 58, 149, 401, 619
Eco52I CGGCCG 1 cut(s) 420
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 259
EcoRII CCWGG 3 cut(s) 142, 315, 375
EcoRV GATATC 1 cut(s) 480
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 2 cut(s) 214, 556
FaiI YATR 5 cut(s) 11, 212, 371, 554, 614
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 4 cut(s) 15, 197, 533, 593
FatI CATG 2 cut(s) 210, 552
FauI CCCGC 2 cut(s) 83, 429
FblI GTMKAC 2 cut(s) 207, 348
Fnu4HI GCNGC 8 cut(s) 156, 290, 293, 308, 367, 420, 434, 626
Fsp4HI GCNGC 8 cut(s) 156, 290, 293, 308, 367, 420, 434, 626
GlaI GCGC 3 cut(s) 38, 216, 551
GluI GCNGC 8 cut(s) 156, 290, 293, 308, 367, 420, 434, 626
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 2 cut(s) 293, 398
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 221, 422
HapII CCGG 6 cut(s) 102, 179, 394, 404, 416, 547
HgaI GACGC 2 cut(s) 99, 160
HhaI GCGC 3 cut(s) 39, 217, 552
Hin1II CATG 2 cut(s) 214, 556
Hin6I GCGC 3 cut(s) 37, 215, 550
HinP1I GCGC 3 cut(s) 37, 215, 550
HincII GTYRAC 2 cut(s) 208, 349
HindII GTYRAC 2 cut(s) 208, 349
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 128, 425
HpaII CCGG 6 cut(s) 102, 179, 394, 404, 416, 547
Hpy166II GTNNAC 4 cut(s) 208, 349, 507, 531
Hpy188I TCNGA 6 cut(s) 82, 127, 165, 193, 401, 430
Hpy188III TCNNGA 5 cut(s) 102, 259, 261, 467, 599
Hpy8I GTNNAC 4 cut(s) 208, 349, 507, 531
Hpy99I CGWCG 5 cut(s) 251, 353, 359, 365, 473
HpyAV CCTTC 2 cut(s) 49, 103
HpyCH4III ACNGT 1 cut(s) 528
HpyCH4IV ACGT 1 cut(s) 533
HpyCH4V TGCA 3 cut(s) 369, 531, 625
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 289, 366, 591
HpyF3I CTNAG 2 cut(s) 26, 338
HpySE526I ACGT 1 cut(s) 533
Hsp92II CATG 2 cut(s) 214, 556
HspAI GCGC 3 cut(s) 37, 215, 550
Kpn2I TCCGGA 1 cut(s) 101
KroI GCCGGC 2 cut(s) 415, 546
KroNI GCCGGC 2 cut(s) 417, 548
KspI CCGCGG 1 cut(s) 486
Kzo9I GATC 2 cut(s) 231, 463
LmnI GCTCC 7 cut(s) 53, 68, 83, 104, 304, 430, 490
Lsp1109I GCAGC 3 cut(s) 279, 353, 420
LweI GCATC 1 cut(s) 324
MaeII ACGT 1 cut(s) 533
MalI GATC 2 cut(s) 233, 465
MbiI CCGCTC 3 cut(s) 48, 63, 78
MboI GATC 2 cut(s) 231, 463
MboII GAAGA 4 cut(s) 34, 108, 192, 487
MhlI GDGCHC 1 cut(s) 533
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 3 cut(s) 105, 171, 188
MroI TCCGGA 1 cut(s) 101
MroNI GCCGGC 2 cut(s) 415, 546
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 6 cut(s) 102, 179, 394, 404, 416, 547
MspR9I CCNGG 5 cut(s) 144, 180, 317, 377, 405
MvaI CCWGG 3 cut(s) 144, 317, 377
MvnI CGCG 2 cut(s) 110, 485
MwoI GCNNNNNNNGC 4 cut(s) 45, 289, 366, 591
NaeI GCCGGC 2 cut(s) 417, 548
NciI CCSGG 2 cut(s) 180, 405
NdeII GATC 2 cut(s) 231, 463
NgoMIV GCCGGC 2 cut(s) 415, 546
NlaIII CATG 2 cut(s) 214, 556
NlaIV GGNNCC 3 cut(s) 100, 106, 306
PaeR7I CTCGAG 1 cut(s) 259
PceI AGGCCT 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PdiI GCCGGC 2 cut(s) 417, 548
PfeI GAWTC 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 393
PkrI GCNGC 8 cut(s) 157, 291, 294, 309, 368, 421, 435, 627
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PshAI GACNNNNGTC 1 cut(s) 170
Psp6I CCWGG 3 cut(s) 142, 315, 375
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 3 cut(s) 142, 315, 375
PspN4I GGNNCC 3 cut(s) 100, 106, 306
PspPI GGNCC 4 cut(s) 58, 149, 401, 619
PstI CTGCAG 1 cut(s) 627
Rsr2I CGGWCCG 1 cut(s) 401
RsrII CGGWCCG 1 cut(s) 401
SacII CCGCGG 1 cut(s) 486
SalI GTCGAC 2 cut(s) 206, 347
SatI GCNGC 8 cut(s) 156, 290, 293, 308, 367, 420, 434, 626
Sau3AI GATC 2 cut(s) 231, 463
Sau96I GGNCC 4 cut(s) 58, 149, 401, 619
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 5 cut(s) 144, 180, 317, 377, 405
SduI GDGCHC 1 cut(s) 533
SfaNI GCATC 1 cut(s) 324
SfcI CTRYAG 1 cut(s) 623
Sfr274I CTCGAG 1 cut(s) 259
Sfr303I CCGCGG 1 cut(s) 486
SgrBI CCGCGG 1 cut(s) 486
SinI GGWCC 4 cut(s) 58, 149, 401, 619
SlaI CTCGAG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
SseBI AGGCCT 1 cut(s) 221
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 5 cut(s) 142, 178, 315, 375, 403
TaaI ACNGT 1 cut(s) 528
TaiI ACGT 1 cut(s) 536
TaqI TCGA 6 cut(s) 207, 234, 260, 348, 456, 567
TauI GCSGC 4 cut(s) 158, 292, 310, 422
TfiI GAWTC 1 cut(s) 128
TseI GCWGC 4 cut(s) 292, 366, 433, 625
TspDTI ATGAA 2 cut(s) 17, 26
TspGWI ACGGA 2 cut(s) 264, 476
VneI GTGCAC 1 cut(s) 529
VpaK11BI GGWCC 4 cut(s) 58, 149, 401, 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XhoI CTCGAG 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 207, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.