MD14G1083200.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007328
Physical Location & Seq
Forward (+)
9822296 .. 9822958
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1083200.v1.1.491

Sequence Viewer

Length: 663 bp
ATGAAGCTCATCAAAATTAGCTCCAAAGATCTCAGTCCGAAACGCCTCTTCCGGTCCAAGAAGGACCGGTCCTCCGTGTCCCGCTCCGATCCGCCGTCTTTCGGTTCCGGAACGGATTCTTCCTCCTCCTCTTCCTCAGACTCAATTCACAAGCCCGGGGCCGGAGCCGGCGGTATCGCCACGCCCACCAGCGTTTTGCCCGAAAAATCGGGTGACTGGTCCGATTTTTCGGCTGATCTCGACATGGCCCAAGCGTTTAAGCTTATAGACCGGGACAACGACGGAGTGGTGTCGAGGAAAGAACTCGAGGCGCTTCTGAGCCGGCTCGGGAACGACCCGCCGAGTCAAGAGGAGGTGACGTTGATGCTGAGCGAGGTGGACCGAGACGGCAACGGTTCGATAACCCTTGAGGCTTTATTGAACCAGGTCGGACCGGTGTCCGGTCCAGCTGCCGACTCGGAGCTTCGGGATGCGTTTGAAGTATTTGACACGGATAACGACGGCAAAATATCGGCGGAGGAACTTTTGAGGGTTTTCACGGCTATCGGGGACGAACGGTGCACGTTAGAGGAGTGCCGGCGTATGATAGACGGCGTAGATAAGGACGGGGATGGGTTCGTGTGCTTTGAGGAGTTTGTTCATATGATGGAACTGCAGAGATGA

Protein Analysis

221

Amino Acids

23.84

Weight (kDa)

4.53

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 81 - 137 4.1e-12 EF-hand domain pair
EF-hand_6 PF13405 82 - 110 1.2e-06 EF-hand domain
EF-hand_7 PF13499 153 - 216 2.1e-13 EF-hand domain pair
EF-hand_1 PF00036 154 - 181 1.5e-08 EF hand domain
EF-hand_6 PF13405 154 - 183 8.6e-09 EF-hand domain
EF-hand_5 PF13202 155 - 178 2.8e-08 EF hand
EF-hand_8 PF13833 168 - 217 1e-06 EF-hand domain pair
EF-hand_1 PF00036 191 - 217 2.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 5 cut(s) 82, 92, 171, 338, 515
AclWI GGATC 1 cut(s) 83
AfiI CCNNNNNNNGG 3 cut(s) 101, 161, 440
AgeI ACCGGT 2 cut(s) 66, 433
AgsI TTSAA 2 cut(s) 421, 479
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 5 cut(s) 7, 21, 262, 449, 463
AluI AGCT 5 cut(s) 7, 21, 262, 449, 463
Alw21I GWGCWC 1 cut(s) 563
Alw26I GTCTC 1 cut(s) 378
Alw44I GTGCAC 1 cut(s) 559
AlwI GGATC 1 cut(s) 83
Ama87I CYCGRG 3 cut(s) 155, 305, 326
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 2 cut(s) 159, 246
ApaLI GTGCAC 1 cut(s) 559
ApeKI GCWGC 1 cut(s) 449
AsiGI ACCGGT 2 cut(s) 66, 433
Asp700I GAANNNNTTC 1 cut(s) 115
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 9 cut(s) 54, 64, 69, 159, 219, 247, 379, 431, 443
AsuC2I CCSGG 3 cut(s) 156, 157, 272
AsuHPI GGTGA 2 cut(s) 224, 367
AvaI CYCGRG 3 cut(s) 155, 305, 326
AvaII GGWCC 7 cut(s) 54, 64, 69, 219, 379, 431, 443
BaeGI GKGCMC 1 cut(s) 563
Bbv12I GWGCWC 1 cut(s) 563
BbvI GCAGC 1 cut(s) 436
BccI CCATC 2 cut(s) 605, 640
BceAI ACGGC 5 cut(s) 79, 403, 517, 555, 607
BciT130I CCWGG 1 cut(s) 425
BcnI CCSGG 3 cut(s) 156, 157, 272
BcoDI GTCTC 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 653
BfoI RGCGCY 1 cut(s) 314
BglII AGATCT 1 cut(s) 28
BisI GCNGC 1 cut(s) 450
BlpI GCTNAGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 451
Bme1390I CCNGG 4 cut(s) 156, 157, 272, 425
Bme18I GGWCC 7 cut(s) 54, 64, 69, 219, 379, 431, 443
BmeT110I CYCGRG 3 cut(s) 155, 305, 326
BmgT120I GGNCC 9 cut(s) 54, 64, 69, 159, 219, 247, 379, 431, 443
BmiI GGNNCC 3 cut(s) 106, 160, 166
BmrFI CCNGG 4 cut(s) 156, 157, 272, 425
BmsI GCATC 2 cut(s) 354, 460
BoxI GACNNNNGTC 1 cut(s) 436
Bpu1102I GCTNAGC 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 3 cut(s) 156, 157, 272
BsaJI CCNNGG 2 cut(s) 155, 156
BsaWI WCCGGW 5 cut(s) 51, 66, 107, 433, 440
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 344, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 161, 440
Bse118I RCCGGY 5 cut(s) 66, 167, 321, 433, 576
Bse1I ACTGG 1 cut(s) 221
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 2 cut(s) 155, 156
BseGI GGATG 2 cut(s) 475, 616
BseLI CCNNNNNNNGG 3 cut(s) 101, 161, 440
BseMII CTCAG 4 cut(s) 46, 150, 308, 359
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 5 cut(s) 115, 118, 365, 584, 644
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 1 cut(s) 436
BshFI GGCC 2 cut(s) 161, 248
BshTI ACCGGT 2 cut(s) 66, 433
BsiHKAI GWGCWC 1 cut(s) 563
BsiHKCI CYCGRG 3 cut(s) 155, 305, 326
BslFI GGGAC 3 cut(s) 64, 287, 563
BslI CCNNNNNNNGG 3 cut(s) 101, 161, 440
BsmAI GTCTC 1 cut(s) 378
BsmBI CGTCTC 1 cut(s) 378
BsmFI GGGAC 3 cut(s) 64, 287, 563
BsnI GGCC 2 cut(s) 161, 248
BsoBI CYCGRG 3 cut(s) 155, 305, 326
Bsp1286I GDGCHC 1 cut(s) 563
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 3 cut(s) 28, 88, 235
Bsp1720I GCTNAGC 1 cut(s) 368
BspACI CCGC 5 cut(s) 82, 92, 171, 338, 515
BspANI GGCC 2 cut(s) 161, 248
BspCNI CTCAG 4 cut(s) 45, 149, 309, 360
BspEI TCCGGA 1 cut(s) 107
BspLI GGNNCC 3 cut(s) 106, 160, 166
BspMAI CTGCAG 1 cut(s) 657
BspPI GGATC 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 84
BsrFI RCCGGY 5 cut(s) 66, 167, 321, 433, 576
BsrI ACTGG 1 cut(s) 221
BssAI RCCGGY 5 cut(s) 66, 167, 321, 433, 576
BssECI CCNNGG 2 cut(s) 155, 156
BssMI GATC 3 cut(s) 28, 88, 235
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 2 cut(s) 395, 558
Bst6I CTCTTC 2 cut(s) 53, 136
BstC8I GCNNGC 3 cut(s) 169, 323, 578
BstDEI CTNAG 4 cut(s) 32, 136, 317, 368
BstF5I GGATG 2 cut(s) 475, 616
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 3 cut(s) 31, 91, 238
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 3 cut(s) 28, 88, 235
BstNI CCWGG 1 cut(s) 425
BstPAI GACNNNNGTC 1 cut(s) 436
BstSCI CCNGG 4 cut(s) 154, 155, 270, 423
BstSFI CTRYAG 1 cut(s) 653
BstSLI GKGCMC 1 cut(s) 563
BstV1I GCAGC 1 cut(s) 436
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 2 cut(s) 161, 248
BtsCI GGATG 2 cut(s) 475, 616
Cac8I GCNNGC 3 cut(s) 169, 323, 578
CfoI GCGC 1 cut(s) 313
Cfr10I RCCGGY 5 cut(s) 66, 167, 321, 433, 576
Cfr13I GGNCC 9 cut(s) 54, 64, 69, 159, 219, 247, 379, 431, 443
Cfr9I CCCGGG 1 cut(s) 155
CpoI CGGWCCG 1 cut(s) 431
CsiI ACCWGGT 1 cut(s) 423
CspAI ACCGGT 2 cut(s) 66, 433
CspI CGGWCCG 1 cut(s) 431
CviAII CATG 1 cut(s) 244
DdeI CTNAG 4 cut(s) 32, 136, 317, 368
DpnI GATC 3 cut(s) 30, 90, 237
DpnII GATC 3 cut(s) 28, 88, 235
Eam1104I CTCTTC 2 cut(s) 53, 136
EarI CTCTTC 2 cut(s) 53, 136
EciI GGCGGA 2 cut(s) 81, 530
Eco47I GGWCC 7 cut(s) 54, 64, 69, 219, 379, 431, 443
Eco88I CYCGRG 3 cut(s) 155, 305, 326
EcoRII CCWGG 1 cut(s) 423
Esp3I CGTCTC 1 cut(s) 378
FaeI CATG 1 cut(s) 247
FaiI YATR 5 cut(s) 245, 266, 584, 642, 644
FaqI GGGAC 3 cut(s) 64, 287, 563
FatI CATG 1 cut(s) 243
FauI CCCGC 2 cut(s) 89, 345
FauNDI CATATG 1 cut(s) 642
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 2 cut(s) 482, 623
Fsp4HI GCNGC 1 cut(s) 450
GlaI GCGC 1 cut(s) 312
GluI GCNGC 1 cut(s) 450
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 2 cut(s) 161, 248
HhaI GCGC 1 cut(s) 313
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 4 cut(s) 116, 140, 343, 455
HphI GGTGA 2 cut(s) 224, 367
Hpy166II GTNNAC 2 cut(s) 379, 561
Hpy188I TCNGA 7 cut(s) 39, 88, 139, 223, 318, 431, 460
Hpy188III TCNNGA 5 cut(s) 108, 239, 328, 347, 467
Hpy8I GTNNAC 2 cut(s) 379, 561
Hpy99I CGWCG 2 cut(s) 284, 503
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 2 cut(s) 395, 558
HpyCH4IV ACGT 2 cut(s) 359, 563
HpyCH4V TGCA 2 cut(s) 561, 655
HpyF3I CTNAG 4 cut(s) 32, 136, 317, 368
HpySE526I ACGT 2 cut(s) 359, 563
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 1 cut(s) 311
Kpn2I TCCGGA 1 cut(s) 107
KroI GCCGGC 3 cut(s) 167, 321, 576
KroNI GCCGGC 3 cut(s) 169, 323, 578
Kzo9I GATC 3 cut(s) 28, 88, 235
LmnI GCTCC 4 cut(s) 26, 89, 164, 460
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 2 cut(s) 354, 460
MabI ACCWGGT 1 cut(s) 423
MaeII ACGT 2 cut(s) 359, 563
MaeIII GTNAC 2 cut(s) 212, 355
MalI GATC 3 cut(s) 30, 90, 237
MbiI CCGCTC 1 cut(s) 84
MboI GATC 3 cut(s) 28, 88, 235
MboII GAAGA 3 cut(s) 40, 111, 123
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 563
MluCI AATT 2 cut(s) 15, 144
MlyI GAGTC 3 cut(s) 134, 352, 449
MmeI TCCRAC 1 cut(s) 409
MroI TCCGGA 1 cut(s) 107
MroNI GCCGGC 3 cut(s) 167, 321, 576
MroXI GAANNNNTTC 1 cut(s) 115
MseI TTAA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 449
MspR9I CCNGG 4 cut(s) 156, 157, 272, 425
MvaI CCWGG 1 cut(s) 425
NaeI GCCGGC 3 cut(s) 169, 323, 578
NciI CCSGG 3 cut(s) 156, 157, 272
NdeI CATATG 1 cut(s) 642
NdeII GATC 3 cut(s) 28, 88, 235
NgoMIV GCCGGC 3 cut(s) 167, 321, 576
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 3 cut(s) 106, 160, 166
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 2 cut(s) 212, 355
PaeR7I CTCGAG 1 cut(s) 305
PdiI GCCGGC 3 cut(s) 169, 323, 578
PdmI GAANNNNTTC 1 cut(s) 115
PfeI GAWTC 1 cut(s) 116
PinAI ACCGGT 2 cut(s) 66, 433
PkrI GCNGC 1 cut(s) 451
PleI GAGTC 3 cut(s) 134, 351, 449
PpsI GAGTC 3 cut(s) 134, 351, 449
PshAI GACNNNNGTC 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 3 cut(s) 106, 160, 166
PspPI GGNCC 9 cut(s) 54, 64, 69, 159, 219, 247, 379, 431, 443
PspXI VCTCGAGB 1 cut(s) 305
PstI CTGCAG 1 cut(s) 657
PsuI RGATCY 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 449
Rsr2I CGGWCCG 1 cut(s) 431
RsrII CGGWCCG 1 cut(s) 431
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 3 cut(s) 28, 88, 235
Sau96I GGNCC 9 cut(s) 54, 64, 69, 159, 219, 247, 379, 431, 443
SchI GAGTC 3 cut(s) 134, 352, 449
ScrFI CCNGG 4 cut(s) 156, 157, 272, 425
SduI GDGCHC 1 cut(s) 563
SexAI ACCWGGT 1 cut(s) 423
SfaNI GCATC 2 cut(s) 354, 460
SfcI CTRYAG 1 cut(s) 653
Sfr274I CTCGAG 1 cut(s) 305
SinI GGWCC 7 cut(s) 54, 64, 69, 219, 379, 431, 443
SlaI CTCGAG 1 cut(s) 305
SmaI CCCGGG 1 cut(s) 157
SmlI CTYRAG 2 cut(s) 305, 407
SmoI CTYRAG 2 cut(s) 305, 407
Sse9I AATT 2 cut(s) 15, 144
SsiI CCGC 5 cut(s) 82, 92, 171, 338, 515
StyD4I CCNGG 4 cut(s) 154, 155, 270, 423
TaaI ACNGT 2 cut(s) 395, 558
TaiI ACGT 2 cut(s) 362, 566
TaqI TCGA 4 cut(s) 240, 293, 306, 398
TaqII GACCGA 1 cut(s) 396
TasI AATT 2 cut(s) 15, 144
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseFI GTSAC 2 cut(s) 212, 355
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 2 cut(s) 212, 355
TspDTI ATGAA 2 cut(s) 17, 629
TspGWI ACGGA 4 cut(s) 64, 128, 297, 506
TspMI CCCGGG 1 cut(s) 155
VneI GTGCAC 1 cut(s) 559
VpaK11BI GGWCC 7 cut(s) 54, 64, 69, 219, 379, 431, 443
XhoI CTCGAG 1 cut(s) 305
XmaI CCCGGG 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.