AT2G43235

transcription by RNA polymerase I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005704
Physical Location & Seq
Reverse (-)
17968698 .. 17970835
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43235.1

Sequence Viewer

Length: 1314 bp
ATGAACATGACTTCGTGTTCTTCTTCATCTTCTTCTTCTTTCCCACGACTCTTCGCCGCCAAATCCCAAATCTATAAAGCTCCAGTTTTATCCTTCTCCACTCCTAAACGCCGTCGTCCTCGTCCTCGCCGGAACCGGAAATCTAATGGCTCATCCTACGACCACACCGACGGCAATCTTCTTTCTATCTCCACTTCCTCTCCCTCCGGAGCCGACGACCAAAGTCTCAGCTTAACCCTAGACGTTCACCGTATTTCAACTCTAGCTAATTACCGCTTCCAATTGTTTCTCGATTCCAGCAAAGACGCGTTCTCCGACTTGCAAACACTAATCTCACTCGACGATAACCGGAGAGTCGTTGTCTCGTGCAAAAAATCAACTATGCAATTCGTCGGCGGTGTGGTTATACTAGGATTCGTATTTGGTTTTGCAATTAGGGTTCTTGTGAAATTAGGATCAGCTTTAAAGGGTAATTTCCAAAGTAATCCCAAATTTGTAGTTAGGAGAGATAGGAGTCTTGGTGGGAAGGAAGTGGTCGTCTCTGTTGATAACATTCGTTCATCTTCAAGAGATTCCAAGAGCTTCATAGCTTCTGATCAAGCTTCTCGAAGTAACTCTACGCCTAGAAATCTCCACCTTAAAGCTCAGAATAATTTGCCGAAATGGTGGCCGACTTCATTGACTAGCCAGAGCTTTGATGTGGTCGACAAAGAAGACTATCAGAGGGAAGCCAACAGAATCGTTCGAGCGATCGTTGATAACAGAACCAGCGGAAAAGACATCACGGATGATGATATTATTCAACTGCGTAGAGTATGTAGAATTTCTGGAGTACAAGTCACTTTTGAGCCAAAAAATACAGGAGATTCCTTTTACAGAACATCTATTGATTTTGTCCTGAACGCTTGCAGCAGAGCTCCGTGGGAGTCTTCTTCTGTTGAGATTTGTAGTGAAGATGCCCGAGAGTTCATTGCTGGGCTCGCTGAGAACATTGGACTTGCAAAGATAGATGCTGCTAGAATGGTTTCTGCAGCAGTTGCAGCACGCACACGTTCATGGTTTCTACAAGCTTGGGCTTTGGAAATACAAGGTAAACATTCTGAATCGGTGGCAGAGCTGTCGAAAATATGCCTCATTCATCGCATATTTCCTCCCAACGAGTACTCTGCTGAGATGGAAATGGTTGCAAGAGGGTTGGAGAAACTCATGAAGCTTGAAGAGCGACAGTCTCTACTGAAAACGTTTGTTGGAATGTGTTGCAGTGAAGATAGTCAAAGAAGCGCAGCTGAGGCTCTGGGTTTGGTGCACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

437

Amino Acids

48.65

Weight (kDa)

9.42

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 705
AccII CGCG 1 cut(s) 308
AccIII TCCGGA 1 cut(s) 206
AciI CCGC 4 cut(s) 57, 274, 396, 771
AclI AACGTT 1 cut(s) 1241
AclWI GGATC 1 cut(s) 463
AcoI YGGCCR 1 cut(s) 668
AcsI RAATTY 2 cut(s) 491, 822
AfaI GTAC 2 cut(s) 834, 1163
AflIII ACRYGT 2 cut(s) 306, 1049
AgsI TTSAA 4 cut(s) 258, 567, 803, 1217
Alw21I GWGCWC 2 cut(s) 919, 1308
Alw26I GTCTC 4 cut(s) 230, 367, 544, 1233
Alw44I GTGCAC 1 cut(s) 1304
AlwI GGATC 1 cut(s) 463
Ama87I CYCGRG 1 cut(s) 960
Aor13HI TCCGGA 1 cut(s) 206
AoxI GGCC 1 cut(s) 668
ApaLI GTGCAC 1 cut(s) 1304
ApeKI GCWGC 5 cut(s) 909, 1013, 1031, 1040, 1283
ApoI RAATTY 2 cut(s) 491, 822
Asp700I GAANNNNTTC 1 cut(s) 1024
AspLEI GCGC 1 cut(s) 1283
AsuHPI GGTGA 1 cut(s) 239
AvaI CYCGRG 1 cut(s) 960
BaeGI GKGCMC 1 cut(s) 1308
BanII GRGCYC 2 cut(s) 919, 981
BarI GAAGNNNNNNTAC 2 cut(s) 601, 633
BauI CACGAG 1 cut(s) 364
BbsI GAAGAC 2 cut(s) 720, 921
Bbv12I GWGCWC 2 cut(s) 919, 1308
BbvCI CCTCAGC 1 cut(s) 1287
BbvI GCAGC 5 cut(s) 921, 1000, 1043, 1052, 1295
BccI CCATC 1 cut(s) 1168
BceAI ACGGC 2 cut(s) 96, 187
BcgI CGANNNNNNTGC 4 cut(s) 1101, 1135, 1148, 1182
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 4 cut(s) 230, 367, 544, 1233
BfaI CTAG 6 cut(s) 239, 263, 410, 624, 684, 1017
BfmI CTRYAG 1 cut(s) 1029
BisI GCNGC 6 cut(s) 57, 910, 1014, 1032, 1041, 1284
BlsI GCNGC 6 cut(s) 58, 911, 1015, 1033, 1042, 1285
BmcAI AGTACT 1 cut(s) 1163
BmeT110I CYCGRG 1 cut(s) 960
BmiI GGNNCC 2 cut(s) 134, 211
BmsI GCATC 2 cut(s) 946, 1000
BoxI GACNNNNGTC 1 cut(s) 222
BpiI GAAGAC 2 cut(s) 720, 921
BpmI CTGGAG 2 cut(s) 66, 849
Bpu10I CCTNAGC 1 cut(s) 1287
BsaJI CCNNGG 1 cut(s) 920
BsaWI WCCGGW 3 cut(s) 135, 206, 348
BsaXI ACNNNNNCTCC 8 cut(s) 184, 214, 296, 326, 505, 535, 822, 852
Bse1I ACTGG 2 cut(s) 83, 1313
Bse3DI GCAATG 1 cut(s) 969
BseAI TCCGGA 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 920
BseGI GGATG 2 cut(s) 152, 793
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 5 cut(s) 241, 659, 975, 1161, 1278
BseNI ACTGG 2 cut(s) 83, 1313
BseSI GKGCMC 1 cut(s) 1308
BseXI GCAGC 5 cut(s) 921, 1000, 1043, 1052, 1295
BseYI CCCAGC 1 cut(s) 974
Bsh1236I CGCG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 753
BshFI GGCC 1 cut(s) 670
BsiEI CGRYCG 1 cut(s) 753
BsiHKAI GWGCWC 2 cut(s) 919, 1308
BsiHKCI CYCGRG 1 cut(s) 960
BsiSI CCGG 4 cut(s) 130, 136, 207, 349
BsmAI GTCTC 4 cut(s) 230, 367, 544, 1233
BsmBI CGTCTC 1 cut(s) 544
BsnI GGCC 1 cut(s) 670
BsoBI CYCGRG 1 cut(s) 960
Bsp1286I GDGCHC 3 cut(s) 919, 981, 1308
Bsp13I TCCGGA 1 cut(s) 206
Bsp143I GATC 3 cut(s) 455, 595, 750
BspACI CCGC 4 cut(s) 57, 274, 396, 771
BspANI GGCC 1 cut(s) 670
BspCNI CTCAG 5 cut(s) 240, 658, 976, 1162, 1279
BspEI TCCGGA 1 cut(s) 206
BspFNI CGCG 1 cut(s) 308
BspHI TCATGA 1 cut(s) 1206
BspLI GGNNCC 2 cut(s) 134, 211
BspMAI CTGCAG 1 cut(s) 1033
BspPI GGATC 1 cut(s) 463
BspQI GCTCTTC 1 cut(s) 1212
BsrDI GCAATG 1 cut(s) 969
BsrI ACTGG 2 cut(s) 83, 1313
BssECI CCNNGG 1 cut(s) 920
BssMI GATC 3 cut(s) 455, 595, 750
BssSI CACGAG 1 cut(s) 364
Bst2BI CACGAG 1 cut(s) 364
Bst4CI ACNGT 2 cut(s) 251, 1227
Bst6I CTCTTC 2 cut(s) 56, 1212
BstAPI GCANNNNNTGC 1 cut(s) 1037
BstC8I GCNNGC 3 cut(s) 907, 981, 1045
BstDEI CTNAG 5 cut(s) 227, 645, 984, 1170, 1287
BstDSI CCRYGG 1 cut(s) 920
BstF5I GGATG 2 cut(s) 152, 793
BstFNI CGCG 1 cut(s) 308
BstHHI GCGC 1 cut(s) 1283
BstKTI GATC 3 cut(s) 458, 598, 753
BstMAI GTCTC 4 cut(s) 230, 367, 544, 1233
BstMBI GATC 3 cut(s) 455, 595, 750
BstMCI CGRYCG 1 cut(s) 753
BstMWI GCNNNNNNNGC 5 cut(s) 980, 1037, 1040, 1219, 1289
BstPAI GACNNNNGTC 1 cut(s) 222
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 1308
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 5 cut(s) 921, 1000, 1043, 1052, 1295
BstV2I GAAGAC 2 cut(s) 720, 921
BsuRI GGCC 1 cut(s) 670
BtgI CCRYGG 1 cut(s) 920
BtgZI GCGATG 1 cut(s) 1124
BtsCI GGATG 2 cut(s) 152, 793
BtsI GCAGTG 1 cut(s) 1267
BtsIMutI CAGTG 2 cut(s) 1267, 1306
Cac8I GCNNGC 3 cut(s) 907, 981, 1045
CciI TCATGA 1 cut(s) 1206
CfoI GCGC 1 cut(s) 1283
CseI GACGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 833, 1162
CviAII CATG 3 cut(s) 7, 1056, 1207
CviQI GTAC 2 cut(s) 833, 1162
DdeI CTNAG 5 cut(s) 227, 645, 984, 1170, 1287
DpnI GATC 3 cut(s) 457, 597, 752
DpnII GATC 3 cut(s) 455, 595, 750
DraI TTTAAA 1 cut(s) 465
EaeI YGGCCR 1 cut(s) 668
Eam1104I CTCTTC 2 cut(s) 56, 1212
EarI CTCTTC 2 cut(s) 56, 1212
Ecl136II GAGCTC 1 cut(s) 917
Eco24I GRGCYC 2 cut(s) 919, 981
Eco53kI GAGCTC 1 cut(s) 917
Eco88I CYCGRG 1 cut(s) 960
EcoICRI GAGCTC 1 cut(s) 917
EcoT38I GRGCYC 2 cut(s) 919, 981
Esp3I CGTCTC 1 cut(s) 544
FaeI CATG 3 cut(s) 10, 1059, 1210
FatI CATG 3 cut(s) 6, 1055, 1206
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 6 cut(s) 57, 910, 1014, 1032, 1041, 1284
FokI GGATG 2 cut(s) 139, 800
FriOI GRGCYC 2 cut(s) 919, 981
Fsp4HI GCNGC 6 cut(s) 57, 910, 1014, 1032, 1041, 1284
FspBI CTAG 6 cut(s) 239, 263, 410, 624, 684, 1017
GlaI GCGC 1 cut(s) 1282
GluI GCNGC 6 cut(s) 57, 910, 1014, 1032, 1041, 1284
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 2 cut(s) 66, 849
HaeIII GGCC 1 cut(s) 670
HapII CCGG 4 cut(s) 130, 136, 207, 349
HgaI GACGC 1 cut(s) 314
HhaI GCGC 1 cut(s) 1283
Hin1II CATG 3 cut(s) 10, 1059, 1210
Hin6I GCGC 1 cut(s) 1281
HinP1I GCGC 1 cut(s) 1281
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 3 cut(s) 600, 1068, 1211
HpaII CCGG 4 cut(s) 130, 136, 207, 349
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 4 cut(s) 247, 706, 1094, 1306
Hpy188I TCNGA 5 cut(s) 316, 595, 648, 723, 1102
Hpy188III TCNNGA 7 cut(s) 207, 290, 567, 606, 828, 898, 1207
Hpy8I GTNNAC 4 cut(s) 247, 706, 1094, 1306
Hpy99I CGWCG 5 cut(s) 117, 173, 218, 344, 395
HpyAV CCTTC 2 cut(s) 103, 520
HpyCH4III ACNGT 2 cut(s) 251, 1227
HpyCH4IV ACGT 3 cut(s) 243, 1051, 1241
HpyF10VI GCNNNNNNNGC 5 cut(s) 980, 1037, 1040, 1219, 1289
HpyF3I CTNAG 5 cut(s) 227, 645, 984, 1170, 1287
HpySE526I ACGT 3 cut(s) 243, 1051, 1241
Hsp92II CATG 3 cut(s) 10, 1059, 1210
HspAI GCGC 1 cut(s) 1281
Kpn2I TCCGGA 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 3 cut(s) 455, 595, 750
LguI GCTCTTC 1 cut(s) 1212
LmnI GCTCC 3 cut(s) 85, 209, 922
Lsp1109I GCAGC 5 cut(s) 921, 1000, 1043, 1052, 1295
LweI GCATC 2 cut(s) 946, 1000
MaeI CTAG 6 cut(s) 239, 263, 410, 624, 684, 1017
MaeII ACGT 3 cut(s) 243, 1051, 1241
MaeIII GTNAC 2 cut(s) 611, 838
MalI GATC 3 cut(s) 457, 597, 752
MboI GATC 3 cut(s) 455, 595, 750
MfeI CAATTG 1 cut(s) 281
MhlI GDGCHC 3 cut(s) 919, 981, 1308
MluCI AATT 9 cut(s) 268, 281, 386, 432, 449, 472, 491, 652, 822
MluI ACGCGT 1 cut(s) 306
MlyI GAGTC 4 cut(s) 42, 363, 523, 935
MmeI TCCRAC 3 cut(s) 339, 1176, 1228
MnlI CCTC 9 cut(s) 129, 135, 208, 214, 717, 1142, 1161, 1184, 1282
MroI TCCGGA 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 1024
MseI TTAA 3 cut(s) 233, 464, 639
MslI CAYNNNNRTG 1 cut(s) 1054
MspA1I CMGCKG 2 cut(s) 771, 1286
MspI CCGG 4 cut(s) 130, 136, 207, 349
MunI CAATTG 1 cut(s) 281
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 5 cut(s) 980, 1037, 1040, 1219, 1289
NdeII GATC 3 cut(s) 455, 595, 750
NlaIII CATG 3 cut(s) 10, 1059, 1210
NlaIV GGNNCC 2 cut(s) 134, 211
NmuCI GTSAC 1 cut(s) 838
PagI TCATGA 1 cut(s) 1206
PciSI GCTCTTC 1 cut(s) 1212
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 312
PdmI GAANNNNTTC 1 cut(s) 1024
PfeI GAWTC 6 cut(s) 293, 414, 572, 738, 866, 1103
PkrI GCNGC 6 cut(s) 58, 911, 1015, 1033, 1042, 1285
Ple19I CGATCG 1 cut(s) 753
PleI GAGTC 4 cut(s) 42, 362, 522, 934
PpsI GAGTC 4 cut(s) 42, 362, 522, 934
PshAI GACNNNNGTC 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 919
Psp1406I AACGTT 1 cut(s) 1241
PspFI CCCAGC 1 cut(s) 974
PspN4I GGNNCC 2 cut(s) 134, 211
PstI CTGCAG 1 cut(s) 1033
PvuI CGATCG 1 cut(s) 753
PvuII CAGCTG 1 cut(s) 1286
RsaI GTAC 2 cut(s) 834, 1163
RsaNI GTAC 2 cut(s) 833, 1162
RseI CAYNNNNRTG 1 cut(s) 1054
SacI GAGCTC 1 cut(s) 919
SalI GTCGAC 1 cut(s) 704
SapI GCTCTTC 1 cut(s) 1212
SaqAI TTAA 3 cut(s) 233, 464, 639
SatI GCNGC 6 cut(s) 57, 910, 1014, 1032, 1041, 1284
Sau3AI GATC 3 cut(s) 455, 595, 750
ScaI AGTACT 1 cut(s) 1163
SchI GAGTC 4 cut(s) 42, 363, 523, 935
SduI GDGCHC 3 cut(s) 919, 981, 1308
SfaNI GCATC 2 cut(s) 946, 1000
SfcI CTRYAG 1 cut(s) 1029
SmiMI CAYNNNNRTG 1 cut(s) 1054
Sse9I AATT 9 cut(s) 268, 281, 386, 432, 449, 472, 491, 652, 822
SsiI CCGC 4 cut(s) 57, 274, 396, 771
SspMI CTAG 6 cut(s) 239, 263, 410, 624, 684, 1017
SstI GAGCTC 1 cut(s) 919
TaaI ACNGT 2 cut(s) 251, 1227
TaiI ACGT 3 cut(s) 246, 1054, 1244
TaqI TCGA 6 cut(s) 291, 339, 607, 705, 745, 1121
TasI AATT 9 cut(s) 268, 281, 386, 432, 449, 472, 491, 652, 822
TatI WGTACW 2 cut(s) 832, 1161
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 6 cut(s) 293, 414, 572, 738, 866, 1103
Tru1I TTAA 3 cut(s) 233, 464, 639
Tru9I TTAA 3 cut(s) 233, 464, 639
TscAI CASTG 2 cut(s) 1267, 1313
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 5 cut(s) 909, 1013, 1031, 1040, 1283
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 9 cut(s) 15, 17, 549, 574, 666, 958, 1044, 1127, 1223
TspGWI ACGGA 2 cut(s) 800, 909
TspRI CASTG 2 cut(s) 1267, 1313
VneI GTGCAC 1 cut(s) 1304
XapI RAATTY 2 cut(s) 491, 822
XmiI GTMKAC 1 cut(s) 705
XmnI GAANNNNTTC 1 cut(s) 1024
XspI CTAG 6 cut(s) 239, 263, 410, 624, 684, 1017
ZrmI AGTACT 1 cut(s) 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.