RLG00000008704

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005704
Physical Location & Seq
Forward (+)
35429360 .. 35433104
3745 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008704

Sequence Viewer

Length: 1374 bp
ATGAACTCCTCCGCCACCGCCACCGCCACCGCCCACTCGCTCGCTCCGTCGCCAATCCACCGCTTCCGCAACCCATGCGCTCCCAAGGCCTCCGTCTCCTGCTCCATGCCTCGCCCTCCCAATCGAAGCCGCCGAAACCGCAGACGAAACTCCAACACCGCCACCACCACCACCACCTTCACCACCGGCCCCACCGCCAAGCCCGCCTTCTACACTTCTTCAGACACTGAAAGCTTCCAAGCCACCTTCGACCTCAACAGCCTCTACCACTCCTCCCACTCCTACCTCCGCCACTTCCTCTCCTCCGCCGCCGACGCCGCCGACGACCTCCAGACCCTCGTCTCCGTCGACGTCGACCGGAGGATCGTCGTCTCCTGCCGCCCCTCCACGCTGCGGTTCGTCGGCAATTTCGTCGTGTTGACCTGCGCCGTCGTGCTGGGGTTTAGGGTTCTGGTTGGGCTGGTGAGGTTAGGGCTTGGGAGCGGGTCGGGTTACGGAAGGGAGAAGGTGGTGACGAGGAGGGATAGGAGTCTTGGCGGGAAAGAGGTTGTGGTAGCGAGGATAGAGAAGCCAAGAGCAGAGGAGGTTAGTGTCAAGAAGAACGGGGGTTTGGGGATTTTGGATAATCCCCTAACGTCGGTGAGGAAGAGCTCAATGGTGGCTGGGATGGAGAGAGTGTTTAAGAATCGGGTTCACTTCGGGGAGAAGCTTCCCCAATGGTGGCCAACTACAACTTCCCAGCCTATTTTAGGGGTTGATAATGAGGAGCACCAGAGAGAAGCAAATAGATTGGTTAGAGCAATCACCGACAATAGAATGAGCGGGAAGGACATCATGGAGGATGATATAATTCATTTACGTCAAATATGCAGGACATATGGAGTACGGGTCTCCTTTGATACTACAAATACACGGGATTCATTGTACCGTGTGTCTGTTGACTTTGTCTTATATGTATGCAGCAGGCGACCGAGTTACTCTAAAGGTGTTGAGATCGAGGGTGAAGATGCTCGGCAATTCATAGCTGGGCTAGCTGAGAACATTGGGCTTGAGAATGTTCGTGCTGGAAGAATTGTGTCTGCCGCCGTGGCTGCGCGTACACGTTCATGTTTTTTGCAGGCTTGGGCTTTAGTAATGCAAGCTAAACATGCAGAAGCAGTAGTAGAACTGTCAAAGATATGCCTTGTTTGGCGGATTTTCCCTCCTGAGGAATCTTCGCCTGAGATGGAGATGGTGGCTCGAGGCTTGGAGAAACACTTGAAAAGGGATCAGAGGGAATTTCTTATGAGCATGCTTGCTGGCACTTGCAGTGAAGAGAGCCAGAGAAGAGCATCAGAAGCGCTGGGTTTGACATCATCTTTCAAAGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

458

Amino Acids

50.65

Weight (kDa)

9.7

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 431
AccBSI CCGCTC 2 cut(s) 483, 822
AccI GTMKAC 2 cut(s) 348, 354
AccII CGCG 1 cut(s) 1096
AclWI GGATC 2 cut(s) 371, 1275
AcoI YGGCCR 1 cut(s) 722
AcsI RAATTY 1 cut(s) 1277
AcuI CTGAAG 1 cut(s) 204
AcyI GRCGYC 2 cut(s) 315, 351
AfaI GTAC 3 cut(s) 885, 926, 1099
AfeI AGCGCT 1 cut(s) 1341
AfiI CCNNNNNNNGG 5 cut(s) 393, 637, 720, 749, 1207
AflIII ACRYGT 1 cut(s) 1100
AgsI TTSAA 2 cut(s) 1261, 1363
AjuI GAANNNNNNNTTGG 6 cut(s) 191, 223, 593, 625, 718, 750
AluBI AGCT 6 cut(s) 234, 651, 709, 1025, 1034, 1142
AluI AGCT 6 cut(s) 234, 651, 709, 1025, 1034, 1142
Alw21I GWGCWC 2 cut(s) 653, 771
Alw26I GTCTC 4 cut(s) 100, 346, 376, 895
AlwI GGATC 2 cut(s) 371, 1275
AlwNI CAGNNNCTG 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 1239
Aor51HI AGCGCT 1 cut(s) 1341
AoxI GGCC 3 cut(s) 87, 187, 722
ApeKI GCWGC 3 cut(s) 391, 960, 1091
ApoI RAATTY 1 cut(s) 1277
AspLEI GCGC 4 cut(s) 80, 428, 1096, 1342
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 6 cut(s) 172, 475, 523, 652, 796, 1013
AsuNHI GCTAGC 1 cut(s) 1030
AvaI CYCGRG 1 cut(s) 1239
AxyI CCTNAGG 1 cut(s) 1206
BalI TGGCCA 1 cut(s) 724
BanII GRGCYC 1 cut(s) 653
Bbv12I GWGCWC 2 cut(s) 653, 771
BbvI GCAGC 3 cut(s) 378, 972, 1078
BccI CCATC 3 cut(s) 661, 1219, 1225
BceAI ACGGC 2 cut(s) 413, 1070
BcgI CGANNNNNNTGC 2 cut(s) 394, 428
BcoDI GTCTC 4 cut(s) 100, 346, 376, 895
BfaI CTAG 1 cut(s) 1031
BfoI RGCGCY 1 cut(s) 1343
BfuAI ACCTGC 1 cut(s) 431
BglI GCCNNNNNGGC 1 cut(s) 1088
BisI GCNGC 8 cut(s) 130, 309, 318, 379, 392, 961, 1083, 1092
BlsI GCNGC 8 cut(s) 131, 310, 319, 380, 393, 962, 1084, 1093
BmeT110I CYCGRG 1 cut(s) 1239
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 190
BmsI GCATC 2 cut(s) 997, 1340
BmtI GCTAGC 1 cut(s) 1034
BoxI GACNNNNGTC 1 cut(s) 338
BpmI CTGGAG 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 1070
BsaHI GRCGYC 2 cut(s) 315, 351
BsaI GGTCTC 1 cut(s) 895
BsaJI CCNNGG 2 cut(s) 84, 1086
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 8 cut(s) 257, 284, 287, 314, 662, 692, 873, 903
Bsc4I CCNNNNNNNGG 5 cut(s) 393, 637, 720, 749, 1207
Bse118I RCCGGY 1 cut(s) 185
Bse21I CCTNAGG 1 cut(s) 1206
BseDI CCNNGG 2 cut(s) 84, 1086
BseGI GGATG 2 cut(s) 672, 847
BseLI CCNNNNNNNGG 5 cut(s) 393, 637, 720, 749, 1207
BseMII CTCAG 3 cut(s) 1026, 1197, 1212
BseRI GAGGAG 5 cut(s) 262, 292, 532, 596, 779
BseXI GCAGC 3 cut(s) 378, 972, 1078
BseYI CCCAGC 5 cut(s) 436, 662, 738, 1025, 1342
Bsh1236I CGCG 1 cut(s) 1096
Bsh1285I CGRYCG 2 cut(s) 358, 971
BshFI GGCC 3 cut(s) 89, 189, 724
BsiEI CGRYCG 2 cut(s) 358, 971
BsiHKAI GWGCWC 2 cut(s) 653, 771
BsiHKCI CYCGRG 1 cut(s) 1239
BsiSI CCGG 2 cut(s) 186, 358
BslI CCNNNNNNNGG 5 cut(s) 393, 637, 720, 749, 1207
BsmAI GTCTC 4 cut(s) 100, 346, 376, 895
BsmBI CGTCTC 3 cut(s) 100, 346, 376
BsnI GGCC 3 cut(s) 89, 189, 724
Bso31I GGTCTC 1 cut(s) 895
BsoBI CYCGRG 1 cut(s) 1239
Bsp1286I GDGCHC 2 cut(s) 653, 771
Bsp143I GATC 3 cut(s) 363, 993, 1267
BspANI GGCC 3 cut(s) 89, 189, 724
BspCNI CTCAG 3 cut(s) 1027, 1198, 1213
BspFNI CGCG 1 cut(s) 1096
BspLI GGNNCC 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 431
BspOI GCTAGC 1 cut(s) 1034
BspPI GGATC 2 cut(s) 371, 1275
BspQI GCTCTTC 2 cut(s) 641, 1321
BspTNI GGTCTC 1 cut(s) 895
BsrBI CCGCTC 2 cut(s) 483, 822
BsrFI RCCGGY 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 84, 1086
BssMI GATC 3 cut(s) 363, 993, 1267
BssNI GRCGYC 2 cut(s) 315, 351
BssT1I CCWWGG 1 cut(s) 84
Bst4CI ACNGT 2 cut(s) 929, 1170
Bst6I CTCTTC 3 cut(s) 641, 1308, 1321
BstACI GRCGYC 2 cut(s) 315, 351
BstAPI GCANNNNNTGC 1 cut(s) 75
BstC8I GCNNGC 9 cut(s) 42, 204, 965, 1032, 1119, 1140, 1292, 1296, 1300
BstDEI CTNAG 3 cut(s) 1035, 1206, 1221
BstDSI CCRYGG 1 cut(s) 1086
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 2 cut(s) 672, 847
BstFNI CGCG 1 cut(s) 1096
BstH2I RGCGCY 1 cut(s) 1343
BstHHI GCGC 4 cut(s) 80, 428, 1096, 1342
BstKTI GATC 3 cut(s) 366, 996, 1270
BstMAI GTCTC 4 cut(s) 100, 346, 376, 895
BstMBI GATC 3 cut(s) 363, 993, 1267
BstMCI CGRYCG 2 cut(s) 358, 971
BstNSI RCATGY 2 cut(s) 1151, 1294
BstPAI GACNNNNGTC 1 cut(s) 338
BstUI CGCG 1 cut(s) 1096
BstV1I GCAGC 3 cut(s) 378, 972, 1078
Bsu36I CCTNAGG 1 cut(s) 1206
BsuRI GGCC 3 cut(s) 89, 189, 724
BtgI CCRYGG 1 cut(s) 1086
BtsCI GGATG 2 cut(s) 672, 847
BtsI GCAGTG 1 cut(s) 1315
BtsIMutI CAGTG 2 cut(s) 225, 1315
BveI ACCTGC 1 cut(s) 431
Cac8I GCNNGC 9 cut(s) 42, 204, 965, 1032, 1119, 1140, 1292, 1296, 1300
CaiI CAGNNNCTG 1 cut(s) 227
CfoI GCGC 4 cut(s) 80, 428, 1096, 1342
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 188
CseI GACGC 1 cut(s) 323
Csp6I GTAC 3 cut(s) 884, 925, 1098
CviAII CATG 6 cut(s) 75, 106, 835, 1107, 1148, 1291
CviQI GTAC 3 cut(s) 884, 925, 1098
DdeI CTNAG 3 cut(s) 1035, 1206, 1221
DpnI GATC 3 cut(s) 365, 995, 1269
DpnII GATC 3 cut(s) 363, 993, 1267
EaeI YGGCCR 1 cut(s) 722
Eam1104I CTCTTC 3 cut(s) 641, 1308, 1321
EarI CTCTTC 3 cut(s) 641, 1308, 1321
EciI GGCGGA 3 cut(s) 278, 295, 1207
Ecl136II GAGCTC 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 84
Eco147I AGGCCT 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 653
Eco31I GGTCTC 1 cut(s) 895
Eco47III AGCGCT 1 cut(s) 1341
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 204
Eco81I CCTNAGG 1 cut(s) 1206
Eco88I CYCGRG 1 cut(s) 1239
EcoICRI GAGCTC 1 cut(s) 651
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoT14I CCWWGG 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 84
Esp3I CGTCTC 3 cut(s) 100, 346, 376
FaeI CATG 6 cut(s) 78, 109, 838, 1110, 1151, 1294
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FatI CATG 6 cut(s) 74, 105, 834, 1106, 1147, 1290
FauI CCCGC 4 cut(s) 211, 476, 530, 815
FauNDI CATATG 1 cut(s) 877
FblI GTMKAC 2 cut(s) 348, 354
Fnu4HI GCNGC 8 cut(s) 130, 309, 318, 379, 392, 961, 1083, 1092
FokI GGATG 2 cut(s) 679, 854
FriOI GRGCYC 1 cut(s) 653
Fsp4HI GCNGC 8 cut(s) 130, 309, 318, 379, 392, 961, 1083, 1092
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 4 cut(s) 79, 427, 1095, 1341
GluI GCNGC 8 cut(s) 130, 309, 318, 379, 392, 961, 1083, 1092
GsaI CCCAGC 5 cut(s) 440, 666, 742, 1029, 1346
GsuI CTGGAG 1 cut(s) 314
HaeII RGCGCY 1 cut(s) 1343
HaeIII GGCC 3 cut(s) 89, 189, 724
HapII CCGG 2 cut(s) 186, 358
HgaI GACGC 1 cut(s) 323
HhaI GCGC 4 cut(s) 80, 428, 1096, 1342
Hin1I GRCGYC 2 cut(s) 315, 351
Hin1II CATG 6 cut(s) 78, 109, 838, 1110, 1151, 1294
Hin6I GCGC 4 cut(s) 78, 426, 1094, 1340
HinP1I GCGC 4 cut(s) 78, 426, 1094, 1340
HincII GTYRAC 4 cut(s) 349, 355, 420, 940
HindII GTYRAC 4 cut(s) 349, 355, 420, 940
HindIII AAGCTT 2 cut(s) 232, 707
HinfI GANTC 4 cut(s) 529, 685, 917, 1211
HpaII CCGG 2 cut(s) 186, 358
HphI GGTGA 6 cut(s) 172, 475, 523, 652, 796, 1013
Hpy166II GTNNAC 6 cut(s) 349, 355, 420, 694, 940, 1100
Hpy188I TCNGA 3 cut(s) 223, 1272, 1336
Hpy188III TCNNGA 3 cut(s) 331, 595, 1205
Hpy8I GTNNAC 6 cut(s) 349, 355, 420, 694, 940, 1100
HpyAV CCTTC 6 cut(s) 187, 217, 256, 492, 499, 820
HpyCH4III ACNGT 2 cut(s) 929, 1170
HpyCH4IV ACGT 4 cut(s) 351, 635, 859, 1102
HpyCH4V TGCA 6 cut(s) 870, 960, 1117, 1138, 1151, 1308
HpyF3I CTNAG 3 cut(s) 1035, 1206, 1221
HpySE526I ACGT 4 cut(s) 351, 635, 859, 1102
Hsp92I GRCGYC 2 cut(s) 315, 351
Hsp92II CATG 6 cut(s) 78, 109, 838, 1110, 1151, 1294
HspAI GCGC 4 cut(s) 78, 426, 1094, 1340
Kzo9I GATC 3 cut(s) 363, 993, 1267
LguI GCTCTTC 2 cut(s) 641, 1321
LmnI GCTCC 5 cut(s) 49, 85, 107, 480, 766
Lsp1109I GCAGC 3 cut(s) 378, 972, 1078
LweI GCATC 2 cut(s) 997, 1340
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 4 cut(s) 351, 635, 859, 1102
MaeIII GTNAC 3 cut(s) 491, 511, 974
MalI GATC 3 cut(s) 365, 995, 1269
MbiI CCGCTC 2 cut(s) 483, 822
MboI GATC 3 cut(s) 363, 993, 1267
MboII GAAGA 8 cut(s) 210, 610, 658, 1016, 1080, 1206, 1325, 1338
MhlI GDGCHC 2 cut(s) 653, 771
MlsI TGGCCA 1 cut(s) 724
MluCI AATT 5 cut(s) 406, 849, 1016, 1071, 1277
MluNI TGGCCA 1 cut(s) 724
MlyI GAGTC 1 cut(s) 538
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 1 cut(s) 724
MscI TGGCCA 1 cut(s) 724
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 1 cut(s) 1105
Msp20I TGGCCA 1 cut(s) 724
MspI CCGG 2 cut(s) 186, 358
MvnI CGCG 1 cut(s) 1096
NdeI CATATG 1 cut(s) 877
NdeII GATC 3 cut(s) 363, 993, 1267
NheI GCTAGC 1 cut(s) 1030
NlaIII CATG 6 cut(s) 78, 109, 838, 1110, 1151, 1294
NlaIV GGNNCC 1 cut(s) 190
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 2 cut(s) 1151, 1294
PaeI GCATGC 1 cut(s) 1294
PaeR7I CTCGAG 1 cut(s) 1239
PceI AGGCCT 1 cut(s) 89
PciSI GCTCTTC 2 cut(s) 641, 1321
PcsI WCGNNNNNNNCGW 5 cut(s) 44, 130, 321, 345, 408
PfeI GAWTC 3 cut(s) 685, 917, 1211
PflFI GACNNNGTC 1 cut(s) 944
PkrI GCNGC 8 cut(s) 131, 310, 319, 380, 393, 962, 1084, 1093
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PshAI GACNNNNGTC 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 653
PspFI CCCAGC 5 cut(s) 436, 662, 738, 1025, 1342
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 1 cut(s) 188
PspXI VCTCGAGB 1 cut(s) 1239
PstNI CAGNNNCTG 1 cut(s) 227
PsyI GACNNNGTC 1 cut(s) 944
RsaI GTAC 3 cut(s) 885, 926, 1099
RsaNI GTAC 3 cut(s) 884, 925, 1098
RseI CAYNNNNRTG 1 cut(s) 1105
SacI GAGCTC 1 cut(s) 653
SalI GTCGAC 2 cut(s) 347, 353
SapI GCTCTTC 2 cut(s) 641, 1321
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 8 cut(s) 130, 309, 318, 379, 392, 961, 1083, 1092
Sau3AI GATC 3 cut(s) 363, 993, 1267
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 1 cut(s) 538
SduI GDGCHC 2 cut(s) 653, 771
SfaNI GCATC 2 cut(s) 997, 1340
Sfr274I CTCGAG 1 cut(s) 1239
SgrDI CGTCGACG 1 cut(s) 347
SlaI CTCGAG 1 cut(s) 1239
SmiMI CAYNNNNRTG 1 cut(s) 1105
SmlI CTYRAG 2 cut(s) 1049, 1239
SmoI CTYRAG 2 cut(s) 1049, 1239
SphI GCATGC 1 cut(s) 1294
Sse9I AATT 5 cut(s) 406, 849, 1016, 1071, 1277
SseBI AGGCCT 1 cut(s) 89
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 1 cut(s) 653
StuI AGGCCT 1 cut(s) 89
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 2 cut(s) 929, 1170
TaiI ACGT 4 cut(s) 354, 638, 862, 1105
TaqI TCGA 6 cut(s) 124, 249, 348, 354, 996, 1240
TaqII GACCGA 1 cut(s) 985
TasI AATT 5 cut(s) 406, 849, 1016, 1071, 1277
TauI GCSGC 5 cut(s) 132, 311, 320, 381, 1085
TfiI GAWTC 3 cut(s) 685, 917, 1211
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 2 cut(s) 232, 1315
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 3 cut(s) 391, 960, 1091
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 5 cut(s) 17, 842, 909, 1009, 1095
TspGWI ACGGA 4 cut(s) 36, 82, 334, 510
TspRI CASTG 2 cut(s) 232, 1315
Tth111I GACNNNGTC 1 cut(s) 944
XagI CCTNNNNNAGG 1 cut(s) 747
XapI RAATTY 1 cut(s) 1277
XceI RCATGY 2 cut(s) 1151, 1294
XhoI CTCGAG 1 cut(s) 1239
XmiI GTMKAC 2 cut(s) 348, 354
XspI CTAG 1 cut(s) 1031
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.