Rh4BG143400

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005704
Physical Location & Seq
Reverse (-)
25506714 .. 25511347
4634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG143400.1

Sequence Viewer

Length: 1383 bp
ATGAACTCCTCCGCCACCGCCACCGCCCACTCTCTCGCTCCGTCGCCATTCCACCGCTTCCGCAACCCATGCTCTCCCAAGGCCTCCTTCTCCTGCTCCATGCCTCGCCCTCCCAATCGAAACCGCCGAAACCGCAGACGAAACTCCAACAGCGCCACTACCACCACCTTCACCACCGTCCCCACCGCCAAGCCCGCCTTCTACACTTCCTCAGACCCTGAAAACATCCAAGCCACCTTCGACCTCAACAGCCTCTACCACTCCTCCCACTCCTACCTCCGCCACTTCCTCGCCTCCGCCGCCGACGCCGCCGACGACCTCCAGACGCTCGTCTCCGTCGACGTCGACCGCAGGATCGTCGTCTCCTGCCGCCCCTCCACGCTGCGGTTCGTCGGCAATTTCGTCGTGTTGACCTGCGCCGTCGTGCTGGGGTTTAGGGTTCTGGTTGGGTTGGTGAGGTTAGGGTTTGGGAGCGGGTCGGGTTACGGAAGGGAGAAGGTGGTGACGAGGAGGGATAGGAGTCTTGGGGGGAAGGAGGTTGTGGTAGCTAGGATAGAGAAGCCAAGAGCAGAGGAGGTTAGTGTCAAGAAGAAAAGCGGTTTGGGGATTTTGGATAATCCCCTATCGTCGGTGAGGAAGAGCTCAATGGTGGCTGGGATGGAGAGAGTGTTTAAGAATCGGGTTCGCTTCGGGGAGAAGCTTCCCCAATGGTGGCCAACTACAACTTCCCAGCCCATTTTAGGGGTTGATAATGAGGAGCACCAGAGAGAAGCAAACAGATTGGTTAGAGCAATCACCGACAATAGAATGAGCGGGAAGGACATTATGGAGGATGATATAATTCATTTACGTCAAATATGCAGGACATATGGAGTACGGGTCTCCTTTGATACTACAAATACACGGGATTCATTGTACCGTGTGTCTGTTGACTTTGTCTTAAATGTATGCAGCAGGGCACCGAGTTACTCTAAAGGTGTTGAGATCGAGGGTGAAGATGCTCGGCAATTCATAGCTGGACTAGCTGAGAACATTGGGCTTGAGAATGTTCGTGCTGGAAGAATTGTATCTGCCGCCGTAGCTGCGCGTACACGTTCATGTTTTTTGCAGGCTTGGGCTTTAGTAATGCAAGCTAAACATGCAGAAGCAGTAGTAGAACTGTCAAATATATGCCTTGTTTGGCGGATTTTCCCTCCTGAGGAATCTTCGCCTGAGATGGAGATGGTGGCTCGAGGCTTGGAGAAACACTTGAAAAAGGATCAGAGGAGATTTCTTATGAGCATGCTTGTTGGCACTTGCAGTGAAGAGAGCCAGAGAAGAGCAGCAGAAGCACTGGGTTTGACATCATCTTTCAAAGGTGTTGGTGATGAACAATATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

460

Amino Acids

51.17

Weight (kDa)

9.68

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 958
AccBSI CCGCTC 2 cut(s) 474, 813
AccI GTMKAC 2 cut(s) 339, 345
AccII CGCG 1 cut(s) 1087
AclWI GGATC 2 cut(s) 362, 1266
AcoI YGGCCR 1 cut(s) 713
AcyI GRCGYC 2 cut(s) 306, 342
AfaI GTAC 3 cut(s) 876, 917, 1090
AfiI CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
AflIII ACRYGT 1 cut(s) 1091
AgsI TTSAA 2 cut(s) 1252, 1354
AjuI GAANNNNNNNTTGG 8 cut(s) 71, 103, 182, 214, 584, 616, 709, 741
AluBI AGCT 7 cut(s) 548, 642, 700, 1016, 1025, 1082, 1133
AluI AGCT 7 cut(s) 548, 642, 700, 1016, 1025, 1082, 1133
Alw21I GWGCWC 2 cut(s) 644, 762
Alw26I GTCTC 3 cut(s) 337, 367, 886
AlwI GGATC 2 cut(s) 362, 1266
AlwNI CAGNNNCTG 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 1230
AoxI GGCC 2 cut(s) 81, 713
ApeKI GCWGC 4 cut(s) 382, 951, 1082, 1322
AspLEI GCGC 3 cut(s) 155, 419, 1087
AsuHPI GGTGA 7 cut(s) 163, 466, 514, 643, 787, 1004, 1376
AvaI CYCGRG 1 cut(s) 1230
AxyI CCTNAGG 1 cut(s) 1197
BaeGI GKGCMC 1 cut(s) 961
BalI TGGCCA 1 cut(s) 715
BanI GGYRCC 1 cut(s) 958
BanII GRGCYC 1 cut(s) 644
Bbv12I GWGCWC 2 cut(s) 644, 762
BbvI GCAGC 4 cut(s) 369, 963, 1069, 1334
BccI CCATC 3 cut(s) 652, 1210, 1216
BceAI ACGGC 2 cut(s) 404, 1061
BcgI CGANNNNNNTGC 4 cut(s) 340, 374, 385, 419
BcoDI GTCTC 3 cut(s) 337, 367, 886
BfaI CTAG 2 cut(s) 549, 1022
BfoI RGCGCY 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 422
BisI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
BlsI GCNGC 8 cut(s) 301, 310, 371, 384, 953, 1075, 1084, 1324
BmeT110I CYCGRG 1 cut(s) 1230
BmiI GGNNCC 1 cut(s) 960
BmrI ACTGGG 1 cut(s) 1343
BmsI GCATC 1 cut(s) 988
BmuI ACTGGG 1 cut(s) 1343
BoxI GACNNNNGTC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 1061
BsaHI GRCGYC 2 cut(s) 306, 342
BsaI GGTCTC 1 cut(s) 886
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 6 cut(s) 248, 278, 653, 683, 864, 894
Bsc4I CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
Bse1I ACTGG 1 cut(s) 1338
Bse21I CCTNAGG 1 cut(s) 1197
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 3 cut(s) 225, 663, 838
BseLI CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
BseMII CTCAG 4 cut(s) 225, 1017, 1188, 1203
BseNI ACTGG 1 cut(s) 1338
BseRI GAGGAG 5 cut(s) 253, 523, 587, 770, 1279
BseSI GKGCMC 1 cut(s) 961
BseXI GCAGC 4 cut(s) 369, 963, 1069, 1334
BseYI CCCAGC 3 cut(s) 427, 653, 729
Bsh1236I CGCG 1 cut(s) 1087
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 2 cut(s) 83, 715
BshNI GGYRCC 1 cut(s) 958
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 644, 762
BsiHKCI CYCGRG 1 cut(s) 1230
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
BsmAI GTCTC 3 cut(s) 337, 367, 886
BsmBI CGTCTC 2 cut(s) 337, 367
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 2 cut(s) 83, 715
Bso31I GGTCTC 1 cut(s) 886
BsoBI CYCGRG 1 cut(s) 1230
Bsp1286I GDGCHC 3 cut(s) 644, 762, 961
Bsp143I GATC 3 cut(s) 354, 984, 1258
BspANI GGCC 2 cut(s) 83, 715
BspCNI CTCAG 4 cut(s) 224, 1018, 1189, 1204
BspFNI CGCG 1 cut(s) 1087
BspLI GGNNCC 1 cut(s) 960
BspMI ACCTGC 1 cut(s) 422
BspPI GGATC 2 cut(s) 362, 1266
BspQI GCTCTTC 2 cut(s) 632, 1312
BspT107I GGYRCC 1 cut(s) 958
BspTNI GGTCTC 1 cut(s) 886
BsrBI CCGCTC 2 cut(s) 474, 813
BsrI ACTGG 1 cut(s) 1338
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 3 cut(s) 354, 984, 1258
BssNI GRCGYC 2 cut(s) 306, 342
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 3 cut(s) 178, 920, 1161
Bst6I CTCTTC 3 cut(s) 632, 1299, 1312
BstACI GRCGYC 2 cut(s) 306, 342
BstAPI GCANNNNNTGC 1 cut(s) 69
BstC8I GCNNGC 4 cut(s) 195, 1110, 1131, 1283
BstDEI CTNAG 4 cut(s) 211, 1026, 1197, 1212
BstF5I GGATG 3 cut(s) 225, 663, 838
BstFNI CGCG 1 cut(s) 1087
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 3 cut(s) 155, 419, 1087
BstKTI GATC 3 cut(s) 357, 987, 1261
BstMAI GTCTC 3 cut(s) 337, 367, 886
BstMBI GATC 3 cut(s) 354, 984, 1258
BstMCI CGRYCG 1 cut(s) 349
BstNSI RCATGY 2 cut(s) 1142, 1285
BstPAI GACNNNNGTC 1 cut(s) 329
BstSLI GKGCMC 1 cut(s) 961
BstUI CGCG 1 cut(s) 1087
BstV1I GCAGC 4 cut(s) 369, 963, 1069, 1334
Bsu36I CCTNAGG 1 cut(s) 1197
BsuRI GGCC 2 cut(s) 83, 715
BtsCI GGATG 3 cut(s) 225, 663, 838
BtsI GCAGTG 1 cut(s) 1306
BtsIMutI CAGTG 2 cut(s) 1306, 1331
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 4 cut(s) 195, 1110, 1131, 1283
CaiI CAGNNNCTG 1 cut(s) 218
CfoI GCGC 3 cut(s) 155, 419, 1087
CseI GACGC 2 cut(s) 314, 334
Csp6I GTAC 3 cut(s) 875, 916, 1089
CviAII CATG 5 cut(s) 69, 100, 1098, 1139, 1282
CviQI GTAC 3 cut(s) 875, 916, 1089
DdeI CTNAG 4 cut(s) 211, 1026, 1197, 1212
DpnI GATC 3 cut(s) 356, 986, 1260
DpnII GATC 3 cut(s) 354, 984, 1258
EaeI YGGCCR 1 cut(s) 713
Eam1104I CTCTTC 3 cut(s) 632, 1299, 1312
EarI CTCTTC 3 cut(s) 632, 1299, 1312
EciI GGCGGA 3 cut(s) 269, 286, 1198
Ecl136II GAGCTC 1 cut(s) 642
Eco130I CCWWGG 1 cut(s) 78
Eco147I AGGCCT 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 644
Eco31I GGTCTC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 642
Eco81I CCTNAGG 1 cut(s) 1197
Eco88I CYCGRG 1 cut(s) 1230
EcoICRI GAGCTC 1 cut(s) 642
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 2 cut(s) 337, 367
FaeI CATG 5 cut(s) 72, 103, 1101, 1142, 1285
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 182, 214
FaqI GGGAC 1 cut(s) 164
FatI CATG 5 cut(s) 68, 99, 1097, 1138, 1281
FauI CCCGC 3 cut(s) 202, 467, 806
FauNDI CATATG 1 cut(s) 868
FblI GTMKAC 2 cut(s) 339, 345
Fnu4HI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
FokI GGATG 3 cut(s) 212, 670, 845
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
FspBI CTAG 2 cut(s) 549, 1022
GlaI GCGC 3 cut(s) 154, 418, 1086
GluI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
GsaI CCCAGC 3 cut(s) 431, 657, 733
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 2 cut(s) 83, 715
HgaI GACGC 2 cut(s) 314, 334
HhaI GCGC 3 cut(s) 155, 419, 1087
Hin1I GRCGYC 2 cut(s) 306, 342
Hin1II CATG 5 cut(s) 72, 103, 1101, 1142, 1285
Hin6I GCGC 3 cut(s) 153, 417, 1085
HinP1I GCGC 3 cut(s) 153, 417, 1085
HincII GTYRAC 4 cut(s) 340, 346, 411, 931
HindII GTYRAC 4 cut(s) 340, 346, 411, 931
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 4 cut(s) 520, 676, 908, 1202
HphI GGTGA 7 cut(s) 163, 466, 514, 643, 787, 1004, 1376
Hpy166II GTNNAC 5 cut(s) 340, 346, 411, 931, 1091
Hpy188I TCNGA 2 cut(s) 214, 1263
Hpy188III TCNNGA 3 cut(s) 322, 586, 1196
Hpy8I GTNNAC 5 cut(s) 340, 346, 411, 931, 1091
HpyAV CCTTC 8 cut(s) 97, 178, 208, 247, 483, 490, 526, 811
HpyCH4III ACNGT 3 cut(s) 178, 920, 1161
HpyCH4IV ACGT 3 cut(s) 342, 850, 1093
HpyCH4V TGCA 6 cut(s) 861, 951, 1108, 1129, 1142, 1299
HpyF3I CTNAG 4 cut(s) 211, 1026, 1197, 1212
HpySE526I ACGT 3 cut(s) 342, 850, 1093
Hsp92I GRCGYC 2 cut(s) 306, 342
Hsp92II CATG 5 cut(s) 72, 103, 1101, 1142, 1285
HspAI GCGC 3 cut(s) 153, 417, 1085
Kzo9I GATC 3 cut(s) 354, 984, 1258
LguI GCTCTTC 2 cut(s) 632, 1312
LmnI GCTCC 4 cut(s) 43, 101, 471, 757
Lsp1109I GCAGC 4 cut(s) 369, 963, 1069, 1334
LweI GCATC 1 cut(s) 988
MaeI CTAG 2 cut(s) 549, 1022
MaeII ACGT 3 cut(s) 342, 850, 1093
MaeIII GTNAC 3 cut(s) 482, 502, 965
MalI GATC 3 cut(s) 356, 986, 1260
MbiI CCGCTC 2 cut(s) 474, 813
MboI GATC 3 cut(s) 354, 984, 1258
MboII GAAGA 7 cut(s) 601, 649, 1007, 1071, 1197, 1316, 1329
MhlI GDGCHC 3 cut(s) 644, 762, 961
MlsI TGGCCA 1 cut(s) 715
MluCI AATT 4 cut(s) 397, 840, 1007, 1062
MluNI TGGCCA 1 cut(s) 715
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 171
Mox20I TGGCCA 1 cut(s) 715
MscI TGGCCA 1 cut(s) 715
MseI TTAA 2 cut(s) 672, 941
MslI CAYNNNNRTG 1 cut(s) 1096
Msp20I TGGCCA 1 cut(s) 715
MvnI CGCG 1 cut(s) 1087
NdeI CATATG 1 cut(s) 868
NdeII GATC 3 cut(s) 354, 984, 1258
NlaIII CATG 5 cut(s) 72, 103, 1101, 1142, 1285
NlaIV GGNNCC 1 cut(s) 960
NmeAIII GCCGAG 1 cut(s) 982
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 2 cut(s) 1142, 1285
PaeI GCATGC 1 cut(s) 1285
PaeR7I CTCGAG 1 cut(s) 1230
PceI AGGCCT 1 cut(s) 83
PciSI GCTCTTC 2 cut(s) 632, 1312
PcsI WCGNNNNNNNCGW 4 cut(s) 124, 312, 336, 399
PfeI GAWTC 3 cut(s) 676, 908, 1202
PflFI GACNNNGTC 1 cut(s) 935
PkrI GCNGC 8 cut(s) 301, 310, 371, 384, 953, 1075, 1084, 1324
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
PshAI GACNNNNGTC 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 644
PspFI CCCAGC 3 cut(s) 427, 653, 729
PspN4I GGNNCC 1 cut(s) 960
PspXI VCTCGAGB 1 cut(s) 1230
PstNI CAGNNNCTG 1 cut(s) 218
PsyI GACNNNGTC 1 cut(s) 935
RsaI GTAC 3 cut(s) 876, 917, 1090
RsaNI GTAC 3 cut(s) 875, 916, 1089
RseI CAYNNNNRTG 1 cut(s) 1096
SacI GAGCTC 1 cut(s) 644
SalI GTCGAC 2 cut(s) 338, 344
SapI GCTCTTC 2 cut(s) 632, 1312
SaqAI TTAA 2 cut(s) 672, 941
SatI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
Sau3AI GATC 3 cut(s) 354, 984, 1258
SchI GAGTC 1 cut(s) 529
SduI GDGCHC 3 cut(s) 644, 762, 961
SfaNI GCATC 1 cut(s) 988
Sfr274I CTCGAG 1 cut(s) 1230
SgrDI CGTCGACG 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 1230
SmiMI CAYNNNNRTG 1 cut(s) 1096
SmlI CTYRAG 2 cut(s) 1040, 1230
SmoI CTYRAG 2 cut(s) 1040, 1230
SphI GCATGC 1 cut(s) 1285
Sse9I AATT 4 cut(s) 397, 840, 1007, 1062
SseBI AGGCCT 1 cut(s) 83
SspMI CTAG 2 cut(s) 549, 1022
SstI GAGCTC 1 cut(s) 644
StuI AGGCCT 1 cut(s) 83
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 3 cut(s) 178, 920, 1161
TaiI ACGT 3 cut(s) 345, 853, 1096
TaqI TCGA 6 cut(s) 118, 240, 339, 345, 987, 1231
TasI AATT 4 cut(s) 397, 840, 1007, 1062
TauI GCSGC 4 cut(s) 302, 311, 372, 1076
TfiI GAWTC 3 cut(s) 676, 908, 1202
Tru1I TTAA 2 cut(s) 672, 941
Tru9I TTAA 2 cut(s) 672, 941
TscAI CASTG 2 cut(s) 1306, 1338
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 4 cut(s) 382, 951, 1082, 1322
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 6 cut(s) 17, 833, 900, 1000, 1086, 1383
TspGWI ACGGA 3 cut(s) 30, 325, 501
TspRI CASTG 2 cut(s) 1306, 1338
Tth111I GACNNNGTC 1 cut(s) 935
XceI RCATGY 2 cut(s) 1142, 1285
XhoI CTCGAG 1 cut(s) 1230
XmiI GTMKAC 2 cut(s) 339, 345
XspI CTAG 2 cut(s) 549, 1022
ZraI GACGTC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.