Rh4CG152300

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005704
Physical Location & Seq
Reverse (-)
33583310 .. 33588002
4693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG152300.1

Sequence Viewer

Length: 1155 bp
ATGAACTCCTCCGCCACCGCCACCGCCACCGCCCACTCGCTCGCTCCGTCGCCATTCCACCGCTTCCGCAACCCATGCGCTCCCAAAGCCTCCTTCTCCTGCTCCATGCCTCGCCCTCCCAATCGAAACCGCCGAAACCGCAGACGAAACTCCGACAGCGCCACCACCGCCACCTTCACCACCGTCCCCACAGCCAAGCCCGCCTTCTACACTTACTCAGACCCTGAAAACATCCAAGCCACCTTCGACCTCAACAGCCTCTACCACTCCTCCCGCTCCTACCTCCGCCACTTCCTCTCCTCCGCGGCCGACGCCGCCGACGACCTCCAGACGCTCGTCTCCGTCGACGTCGACCGGAGGATCGTCGTCTCCTGCCGCCCCTCCACGCTGCGGTTCGTCGGCAATTTCGTCGTGTTGACCTGCGCCGTCGTGCTTGGGTTTAGGGTTCTGGTTGGGTTGGTGAGGTTAGGGTTTGGGAGCGGGTCGGGTTACGGAAGGGAGAAGGTGGTGACAAGGAGGGATAGGAGTCTTGGGGGGAAGGAGGTTGTGGTAGCTAGGATAGAGAAGCCGAGAGCAGAGGAGGTTAGTGTCAAGAAGAACAGCGGTTTGGGGATTTTGGATAATCCCCTATCGTCGGTGAGGAAGAGCTCAATGGTGGCTGGGATGGAGAGAGTGTTTAAGAATCGGGTTCGCTTCGGGGAGAAGCTTCCCCAATGGTGGCCAACTACAACGTCCCAGCCCATTTTAGGGGTTGATAATGAGGAGCACCAGAGAGAAGCAAATAGATTGGTTAGAGCAATCACCGACAATAGAATGAGCGGGAAGGACATTATGGAGGATGATATAATTCATTTACGTCAAATATGCAGGACATATGGAGTACGGGTCTCCTTTGATACTACAAATACACGGGATTCATTGTACCGTGTGTCTGTTGACTTTGTCTTCAATGTATGCAGCAGGACACCGAGTTACTCTAAAGGTGTTGAGATCGAGGGTGAAGATGCCCGGCAATTCATAGCTGGGCTAGCTGAGAACATTGGGCTTGAGAATGTTCGTGCTGGAAGAATTGTGTCTGCCGCCGTAGCTGCGCGTACACGTTCATGTTTTTTGCAGGCTTGGGTAATTTTCCCACTGTTGCTCTTTTTACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

384

Amino Acids

42.73

Weight (kDa)

10.02

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
Acc36I ACCTGC 1 cut(s) 428
AccBSI CCGCTC 3 cut(s) 276, 480, 819
AccI GTMKAC 2 cut(s) 345, 351
AccII CGCG 2 cut(s) 305, 1093
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 2 cut(s) 306, 719
AcyI GRCGYC 2 cut(s) 312, 348
AfaI GTAC 3 cut(s) 882, 923, 1096
AfiI CCNNNNNNNGG 5 cut(s) 390, 634, 717, 746, 747
AflIII ACRYGT 1 cut(s) 1097
AgsI TTSAA 1 cut(s) 949
AjuI GAANNNNNNNTTGG 6 cut(s) 77, 109, 188, 220, 590, 622
AluBI AGCT 6 cut(s) 554, 648, 706, 1022, 1031, 1088
AluI AGCT 6 cut(s) 554, 648, 706, 1022, 1031, 1088
Alw21I GWGCWC 2 cut(s) 650, 768
Alw26I GTCTC 3 cut(s) 343, 373, 892
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 2 cut(s) 306, 719
ApeKI GCWGC 3 cut(s) 388, 957, 1088
AspLEI GCGC 4 cut(s) 80, 161, 425, 1093
AsuC2I CCSGG 1 cut(s) 1009
AsuHPI GGTGA 6 cut(s) 169, 472, 520, 649, 793, 1010
AsuNHI GCTAGC 1 cut(s) 1027
BalI TGGCCA 1 cut(s) 721
BanII GRGCYC 1 cut(s) 650
BbsI GAAGAC 1 cut(s) 937
Bbv12I GWGCWC 2 cut(s) 650, 768
BbvI GCAGC 3 cut(s) 375, 969, 1075
BccI CCATC 1 cut(s) 658
BceAI ACGGC 2 cut(s) 410, 1067
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BcnI CCSGG 1 cut(s) 1009
BcoDI GTCTC 3 cut(s) 343, 373, 892
BfaI CTAG 2 cut(s) 555, 1028
BfoI RGCGCY 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 428
BisI GCNGC 7 cut(s) 306, 315, 376, 389, 958, 1080, 1089
BlsI GCNGC 7 cut(s) 307, 316, 377, 390, 959, 1081, 1090
Bme1390I CCNGG 1 cut(s) 1009
BmrFI CCNGG 1 cut(s) 1009
BmsI GCATC 1 cut(s) 994
BmtI GCTAGC 1 cut(s) 1031
BoxI GACNNNNGTC 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 937
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 1067
BpuMI CCSGG 1 cut(s) 1009
BsaHI GRCGYC 2 cut(s) 312, 348
BsaI GGTCTC 1 cut(s) 892
BsaJI CCNNGG 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 8 cut(s) 254, 281, 284, 311, 659, 689, 870, 900
Bsc4I CCNNNNNNNGG 5 cut(s) 390, 634, 717, 746, 747
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 3 cut(s) 231, 669, 844
BseLI CCNNNNNNNGG 5 cut(s) 390, 634, 717, 746, 747
BseMII CTCAG 2 cut(s) 231, 1023
BseRI GAGGAG 4 cut(s) 259, 289, 593, 776
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 3 cut(s) 375, 969, 1075
BseYI CCCAGC 3 cut(s) 659, 735, 1022
Bsh1236I CGCG 2 cut(s) 305, 1093
Bsh1285I CGRYCG 2 cut(s) 309, 355
BshFI GGCC 2 cut(s) 308, 721
BsiEI CGRYCG 2 cut(s) 309, 355
BsiHKAI GWGCWC 2 cut(s) 650, 768
BsiSI CCGG 2 cut(s) 355, 1009
BslFI GGGAC 2 cut(s) 170, 718
BslI CCNNNNNNNGG 5 cut(s) 390, 634, 717, 746, 747
BsmAI GTCTC 3 cut(s) 343, 373, 892
BsmBI CGTCTC 2 cut(s) 343, 373
BsmFI GGGAC 2 cut(s) 170, 718
BsnI GGCC 2 cut(s) 308, 721
Bso31I GGTCTC 1 cut(s) 892
Bsp1286I GDGCHC 2 cut(s) 650, 768
Bsp143I GATC 2 cut(s) 360, 990
BspANI GGCC 2 cut(s) 308, 721
BspCNI CTCAG 2 cut(s) 230, 1024
BspFNI CGCG 2 cut(s) 305, 1093
BspMI ACCTGC 1 cut(s) 428
BspOI GCTAGC 1 cut(s) 1031
BspPI GGATC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 638
BspTNI GGTCTC 1 cut(s) 892
BsrBI CCGCTC 3 cut(s) 276, 480, 819
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 2 cut(s) 360, 990
BssNI GRCGYC 2 cut(s) 312, 348
Bst4CI ACNGT 4 cut(s) 184, 926, 1137, 1152
Bst6I CTCTTC 1 cut(s) 638
BstACI GRCGYC 2 cut(s) 312, 348
BstAPI GCANNNNNTGC 1 cut(s) 75
BstC8I GCNNGC 4 cut(s) 42, 201, 1029, 1116
BstDEI CTNAG 2 cut(s) 217, 1032
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 3 cut(s) 231, 669, 844
BstFNI CGCG 2 cut(s) 305, 1093
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 4 cut(s) 80, 161, 425, 1093
BstKTI GATC 2 cut(s) 363, 993
BstMAI GTCTC 3 cut(s) 343, 373, 892
BstMBI GATC 2 cut(s) 360, 990
BstMCI CGRYCG 2 cut(s) 309, 355
BstPAI GACNNNNGTC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 1007
BstUI CGCG 2 cut(s) 305, 1093
BstV1I GCAGC 3 cut(s) 375, 969, 1075
BstV2I GAAGAC 1 cut(s) 937
BstZI CGGCCG 1 cut(s) 306
BsuRI GGCC 2 cut(s) 308, 721
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 3 cut(s) 231, 669, 844
BtsIMutI CAGTG 1 cut(s) 1133
BveI ACCTGC 1 cut(s) 428
Cac8I GCNNGC 4 cut(s) 42, 201, 1029, 1116
CaiI CAGNNNCTG 1 cut(s) 224
CfoI GCGC 4 cut(s) 80, 161, 425, 1093
Cfr42I CCGCGG 1 cut(s) 306
CseI GACGC 2 cut(s) 320, 340
Csp6I GTAC 3 cut(s) 881, 922, 1095
CviAII CATG 3 cut(s) 75, 106, 1104
CviQI GTAC 3 cut(s) 881, 922, 1095
DdeI CTNAG 2 cut(s) 217, 1032
DpnI GATC 2 cut(s) 362, 992
DpnII GATC 2 cut(s) 360, 990
EaeI YGGCCR 2 cut(s) 306, 719
EagI CGGCCG 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 638
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 648
EclXI CGGCCG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 650
Eco31I GGTCTC 1 cut(s) 892
Eco52I CGGCCG 1 cut(s) 306
Eco53kI GAGCTC 1 cut(s) 648
EcoICRI GAGCTC 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 650
Esp3I CGTCTC 2 cut(s) 343, 373
FaeI CATG 3 cut(s) 78, 109, 1107
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FaqI GGGAC 2 cut(s) 170, 718
FatI CATG 3 cut(s) 74, 105, 1103
FauI CCCGC 4 cut(s) 208, 281, 473, 812
FauNDI CATATG 1 cut(s) 874
FblI GTMKAC 2 cut(s) 345, 351
Fnu4HI GCNGC 7 cut(s) 306, 315, 376, 389, 958, 1080, 1089
FokI GGATG 3 cut(s) 218, 676, 851
FriOI GRGCYC 1 cut(s) 650
Fsp4HI GCNGC 7 cut(s) 306, 315, 376, 389, 958, 1080, 1089
FspBI CTAG 2 cut(s) 555, 1028
GlaI GCGC 4 cut(s) 79, 160, 424, 1092
GluI GCNGC 7 cut(s) 306, 315, 376, 389, 958, 1080, 1089
GsaI CCCAGC 3 cut(s) 663, 739, 1026
GsuI CTGGAG 1 cut(s) 311
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 308, 721
HapII CCGG 2 cut(s) 355, 1009
HgaI GACGC 2 cut(s) 320, 340
HhaI GCGC 4 cut(s) 80, 161, 425, 1093
Hin1I GRCGYC 2 cut(s) 312, 348
Hin1II CATG 3 cut(s) 78, 109, 1107
Hin6I GCGC 4 cut(s) 78, 159, 423, 1091
HinP1I GCGC 4 cut(s) 78, 159, 423, 1091
HincII GTYRAC 4 cut(s) 346, 352, 417, 937
HindII GTYRAC 4 cut(s) 346, 352, 417, 937
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 3 cut(s) 526, 682, 914
HpaII CCGG 2 cut(s) 355, 1009
HphI GGTGA 6 cut(s) 169, 472, 520, 649, 793, 1010
Hpy166II GTNNAC 5 cut(s) 346, 352, 417, 937, 1097
Hpy188I TCNGA 2 cut(s) 154, 220
Hpy188III TCNNGA 2 cut(s) 328, 592
Hpy8I GTNNAC 5 cut(s) 346, 352, 417, 937, 1097
HpyAV CCTTC 8 cut(s) 103, 184, 214, 253, 489, 496, 532, 817
HpyCH4III ACNGT 4 cut(s) 184, 926, 1137, 1152
HpyCH4IV ACGT 4 cut(s) 348, 731, 856, 1099
HpyCH4V TGCA 3 cut(s) 867, 957, 1114
HpyF3I CTNAG 2 cut(s) 217, 1032
HpySE526I ACGT 4 cut(s) 348, 731, 856, 1099
Hsp92I GRCGYC 2 cut(s) 312, 348
Hsp92II CATG 3 cut(s) 78, 109, 1107
HspAI GCGC 4 cut(s) 78, 159, 423, 1091
KspI CCGCGG 1 cut(s) 306
Kzo9I GATC 2 cut(s) 360, 990
LguI GCTCTTC 1 cut(s) 638
LmnI GCTCC 6 cut(s) 49, 85, 107, 281, 477, 763
Lsp1109I GCAGC 3 cut(s) 375, 969, 1075
LweI GCATC 1 cut(s) 994
MaeI CTAG 2 cut(s) 555, 1028
MaeII ACGT 4 cut(s) 348, 731, 856, 1099
MaeIII GTNAC 3 cut(s) 488, 508, 971
MalI GATC 2 cut(s) 362, 992
MbiI CCGCTC 3 cut(s) 276, 480, 819
MboI GATC 2 cut(s) 360, 990
MboII GAAGA 5 cut(s) 607, 655, 937, 1013, 1077
MhlI GDGCHC 2 cut(s) 650, 768
MlsI TGGCCA 1 cut(s) 721
MluCI AATT 5 cut(s) 403, 846, 1013, 1068, 1125
MluNI TGGCCA 1 cut(s) 721
MlyI GAGTC 1 cut(s) 535
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 1 cut(s) 721
MscI TGGCCA 1 cut(s) 721
MseI TTAA 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 1102
Msp20I TGGCCA 1 cut(s) 721
MspA1I CMGCKG 2 cut(s) 305, 603
MspI CCGG 2 cut(s) 355, 1009
MspR9I CCNGG 1 cut(s) 1009
MvnI CGCG 2 cut(s) 305, 1093
NciI CCSGG 1 cut(s) 1009
NdeI CATATG 1 cut(s) 874
NdeII GATC 2 cut(s) 360, 990
NheI GCTAGC 1 cut(s) 1027
NlaIII CATG 3 cut(s) 78, 109, 1107
NmeAIII GCCGAG 1 cut(s) 594
NmuCI GTSAC 1 cut(s) 508
PciSI GCTCTTC 1 cut(s) 638
PcsI WCGNNNNNNNCGW 5 cut(s) 44, 130, 318, 342, 405
PfeI GAWTC 2 cut(s) 682, 914
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 7 cut(s) 307, 316, 377, 390, 959, 1081, 1090
PleI GAGTC 1 cut(s) 534
PpsI GAGTC 1 cut(s) 534
PshAI GACNNNNGTC 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 650
PspFI CCCAGC 3 cut(s) 659, 735, 1022
PstNI CAGNNNCTG 1 cut(s) 224
PsyI GACNNNGTC 1 cut(s) 941
RsaI GTAC 3 cut(s) 882, 923, 1096
RsaNI GTAC 3 cut(s) 881, 922, 1095
RseI CAYNNNNRTG 1 cut(s) 1102
SacI GAGCTC 1 cut(s) 650
SacII CCGCGG 1 cut(s) 306
SalI GTCGAC 2 cut(s) 344, 350
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 1 cut(s) 678
SatI GCNGC 7 cut(s) 306, 315, 376, 389, 958, 1080, 1089
Sau3AI GATC 2 cut(s) 360, 990
SchI GAGTC 1 cut(s) 535
ScrFI CCNGG 1 cut(s) 1009
SduI GDGCHC 2 cut(s) 650, 768
SfaNI GCATC 1 cut(s) 994
Sfr303I CCGCGG 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 306
SgrDI CGTCGACG 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 1102
SmlI CTYRAG 1 cut(s) 1046
SmoI CTYRAG 1 cut(s) 1046
Sse9I AATT 5 cut(s) 403, 846, 1013, 1068, 1125
SspMI CTAG 2 cut(s) 555, 1028
SstI GAGCTC 1 cut(s) 650
StyD4I CCNGG 1 cut(s) 1007
TaaI ACNGT 4 cut(s) 184, 926, 1137, 1152
TaiI ACGT 4 cut(s) 351, 734, 859, 1102
TaqI TCGA 5 cut(s) 124, 246, 345, 351, 993
TasI AATT 5 cut(s) 403, 846, 1013, 1068, 1125
TauI GCSGC 4 cut(s) 308, 317, 378, 1082
TfiI GAWTC 2 cut(s) 682, 914
Tru1I TTAA 1 cut(s) 678
Tru9I TTAA 1 cut(s) 678
TscAI CASTG 1 cut(s) 1140
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 3 cut(s) 388, 957, 1088
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 5 cut(s) 17, 839, 906, 1006, 1092
TspGWI ACGGA 3 cut(s) 36, 331, 507
TspRI CASTG 1 cut(s) 1140
Tth111I GACNNNGTC 1 cut(s) 941
XmiI GTMKAC 2 cut(s) 345, 351
XspI CTAG 2 cut(s) 555, 1028
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.