Rmu_sc0006439.1_g000002

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006439.1
OG0005704
Physical Location & Seq
Reverse (-)
5432 .. 8990
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006439.1_g000002.1.cds

Sequence Viewer

Length: 1383 bp
atgaactcctccgccaccgccaccgcccactcgctcgctccgtcgccattccaccgcttccgcaacccatgcgctcccaaagcctccttctcctgctccatgcctcgccctcccaatcgaaaccgccgaaaccgcagacgaaactccgacagcgccaccaccgccaccttcaccaccgtccacaccgccaagcccgccttctacacttcctcagaccctgaaaacatccaagccaccttcgacctcaacagcctctaccactcctcccgctcctacctccgccacttcctctcctccgccgccgacgccgccgacgacctccagaccctcgtctccgtcgacgtcgaccggaggatcgtcgtctcctgccgcccctccacgctgcggttcgtcggcaatttcgtcgtgttgacctgcgccgtcgtgctgggttttagggttctggttgggctggtgaggttagggtttgggagcgggtcgggttacggaagggagaaggtggtgacgaggagggataggagtcttgggggaaaggaggttgtggtagctaggatagagaagccaagagcagaggaggttagtgtcaagaagaacagcggtttggggattttggataatcccctgtcgtcggtgaggaagagctcaatggtggctgggatggagagagtgtttaagaatcgggttcgcttcggggagaagcttccccaatggtggccaactacaacttcccggcccattttaggggttggtaatgaggagcaccagagagaagcaaacagattggttagagcaatcaccgacaatagaatgagcgggaaggacattatggaggatgatataattcatttacgtcaaatatgcaggacatatggagtacgggtctcctttgatactacaaatacacgggattcattgtaccgtgtgtctgttgactttgtcttacatgtatgcagcaggacaccgagttactctaaaggtgttgagatcgagggtgaagatgcccggcaattcatagctgggctagctgagaacattgggcttgagaatgttcgtgctggaagaattgtgtctgccgccgtagctgcacgtacacgttcatgttttttgcaggcttgggctttagtaatgcaagctaaacatgcagaagccgtagtagaactgtcaaaaatatgccttgtttggcggattttccctcccgaggaatcttcgcctgagatggagatggtggctcgaggcttggagaaacacttgaaaagggatcagaggagatttcttatgagcatgcttgttggcacttgcagtgaagagagccagagaagagcagcagaagcactgggtttggcatcatctttcaaaggtgttggtggtgaacaatatcaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

460

Amino Acids

51.16

Weight (kDa)

9.87

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 422
AccBSI CCGCTC 3 cut(s) 270, 474, 813
AccI GTMKAC 2 cut(s) 339, 345
AclWI GGATC 2 cut(s) 362, 1266
AcoI YGGCCR 1 cut(s) 713
AcyI GRCGYC 2 cut(s) 306, 342
AfaI GTAC 3 cut(s) 876, 917, 1090
AfiI CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
AflIII ACRYGT 2 cut(s) 943, 1091
AgsI TTSAA 2 cut(s) 1252, 1354
AjuI GAANNNNNNNTTGG 8 cut(s) 71, 103, 182, 214, 584, 616, 709, 741
AluBI AGCT 7 cut(s) 548, 642, 700, 1016, 1025, 1082, 1133
AluI AGCT 7 cut(s) 548, 642, 700, 1016, 1025, 1082, 1133
Alw21I GWGCWC 2 cut(s) 644, 762
Alw26I GTCTC 3 cut(s) 337, 367, 886
AlwI GGATC 2 cut(s) 362, 1266
AlwNI CAGNNNCTG 1 cut(s) 218
Ama87I CYCGRG 2 cut(s) 1196, 1230
AoxI GGCC 2 cut(s) 713, 731
ApeKI GCWGC 4 cut(s) 382, 951, 1082, 1322
AspLEI GCGC 3 cut(s) 74, 155, 419
AspS9I GGNCC 1 cut(s) 732
AsuC2I CCSGG 2 cut(s) 730, 1003
AsuHPI GGTGA 7 cut(s) 163, 466, 514, 643, 787, 1004, 1379
AsuNHI GCTAGC 1 cut(s) 1021
AvaI CYCGRG 2 cut(s) 1196, 1230
BalI TGGCCA 1 cut(s) 715
BanII GRGCYC 1 cut(s) 644
Bbv12I GWGCWC 2 cut(s) 644, 762
BbvI GCAGC 4 cut(s) 369, 963, 1069, 1334
BccI CCATC 3 cut(s) 652, 1210, 1216
BceAI ACGGC 3 cut(s) 404, 1061, 1133
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BcnI CCSGG 2 cut(s) 730, 1003
BcoDI GTCTC 3 cut(s) 337, 367, 886
BfaI CTAG 2 cut(s) 549, 1022
BfoI RGCGCY 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 422
BisI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
BlsI GCNGC 8 cut(s) 301, 310, 371, 384, 953, 1075, 1084, 1324
Bme1390I CCNGG 2 cut(s) 730, 1003
BmeT110I CYCGRG 2 cut(s) 1196, 1230
BmgT120I GGNCC 1 cut(s) 732
BmrFI CCNGG 2 cut(s) 730, 1003
BmrI ACTGGG 1 cut(s) 1343
BmsI GCATC 2 cut(s) 988, 1352
BmtI GCTAGC 1 cut(s) 1025
BmuI ACTGGG 1 cut(s) 1343
BoxI GACNNNNGTC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 1061
BpuMI CCSGG 2 cut(s) 730, 1003
BsaAI YACGTR 1 cut(s) 1088
BsaHI GRCGYC 2 cut(s) 306, 342
BsaI GGTCTC 1 cut(s) 886
BsaJI CCNNGG 1 cut(s) 1197
BsaWI WCCGGW 1 cut(s) 348
BsaXI ACNNNNNCTCC 8 cut(s) 248, 275, 278, 305, 653, 683, 864, 894
Bsc4I CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
Bse1I ACTGG 1 cut(s) 1338
BseDI CCNNGG 1 cut(s) 1197
BseGI GGATG 3 cut(s) 225, 663, 838
BseLI CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
BseMII CTCAG 3 cut(s) 225, 1017, 1203
BseNI ACTGG 1 cut(s) 1338
BseRI GAGGAG 6 cut(s) 253, 283, 523, 587, 770, 1279
BseXI GCAGC 4 cut(s) 369, 963, 1069, 1334
BseYI CCCAGC 3 cut(s) 427, 653, 1016
BsgI GTGCAG 1 cut(s) 1068
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 2 cut(s) 715, 733
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 644, 762
BsiHKCI CYCGRG 2 cut(s) 1196, 1230
BsiSI CCGG 3 cut(s) 349, 730, 1003
BslI CCNNNNNNNGG 6 cut(s) 384, 628, 711, 740, 741, 1198
BsmAI GTCTC 3 cut(s) 337, 367, 886
BsmBI CGTCTC 2 cut(s) 337, 367
BsnI GGCC 2 cut(s) 715, 733
Bso31I GGTCTC 1 cut(s) 886
BsoBI CYCGRG 2 cut(s) 1196, 1230
Bsp1286I GDGCHC 2 cut(s) 644, 762
Bsp143I GATC 3 cut(s) 354, 984, 1258
BspANI GGCC 2 cut(s) 715, 733
BspCNI CTCAG 3 cut(s) 224, 1018, 1204
BspMI ACCTGC 1 cut(s) 422
BspOI GCTAGC 1 cut(s) 1025
BspPI GGATC 2 cut(s) 362, 1266
BspQI GCTCTTC 2 cut(s) 632, 1312
BspTNI GGTCTC 1 cut(s) 886
BsrBI CCGCTC 3 cut(s) 270, 474, 813
BsrI ACTGG 1 cut(s) 1338
BssECI CCNNGG 1 cut(s) 1197
BssMI GATC 3 cut(s) 354, 984, 1258
BssNI GRCGYC 2 cut(s) 306, 342
Bst4CI ACNGT 3 cut(s) 178, 920, 1161
Bst6I CTCTTC 3 cut(s) 632, 1299, 1312
BstACI GRCGYC 2 cut(s) 306, 342
BstAPI GCANNNNNTGC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 1088
BstC8I GCNNGC 6 cut(s) 36, 195, 1023, 1110, 1131, 1283
BstDEI CTNAG 3 cut(s) 211, 1026, 1212
BstF5I GGATG 3 cut(s) 225, 663, 838
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 3 cut(s) 74, 155, 419
BstKTI GATC 3 cut(s) 357, 987, 1261
BstMAI GTCTC 3 cut(s) 337, 367, 886
BstMBI GATC 3 cut(s) 354, 984, 1258
BstMCI CGRYCG 1 cut(s) 349
BstNSI RCATGY 3 cut(s) 947, 1142, 1285
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 728, 1001
BstV1I GCAGC 4 cut(s) 369, 963, 1069, 1334
BsuRI GGCC 2 cut(s) 715, 733
BtsCI GGATG 3 cut(s) 225, 663, 838
BtsI GCAGTG 1 cut(s) 1306
BtsIMutI CAGTG 2 cut(s) 1306, 1331
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 6 cut(s) 36, 195, 1023, 1110, 1131, 1283
CaiI CAGNNNCTG 1 cut(s) 218
CfoI GCGC 3 cut(s) 74, 155, 419
Cfr13I GGNCC 1 cut(s) 732
CseI GACGC 1 cut(s) 314
Csp6I GTAC 3 cut(s) 875, 916, 1089
CviAII CATG 6 cut(s) 69, 100, 944, 1098, 1139, 1282
CviQI GTAC 3 cut(s) 875, 916, 1089
DdeI CTNAG 3 cut(s) 211, 1026, 1212
DpnI GATC 3 cut(s) 356, 986, 1260
DpnII GATC 3 cut(s) 354, 984, 1258
EaeI YGGCCR 1 cut(s) 713
Eam1104I CTCTTC 3 cut(s) 632, 1299, 1312
EarI CTCTTC 3 cut(s) 632, 1299, 1312
EciI GGCGGA 3 cut(s) 269, 286, 1198
Ecl136II GAGCTC 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 644
Eco31I GGTCTC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 642
Eco88I CYCGRG 2 cut(s) 1196, 1230
EcoICRI GAGCTC 1 cut(s) 642
EcoT38I GRGCYC 1 cut(s) 644
Esp3I CGTCTC 2 cut(s) 337, 367
FaeI CATG 6 cut(s) 72, 103, 947, 1101, 1142, 1285
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 6 cut(s) 68, 99, 943, 1097, 1138, 1281
FauI CCCGC 4 cut(s) 202, 275, 467, 806
FauNDI CATATG 1 cut(s) 868
FblI GTMKAC 2 cut(s) 339, 345
Fnu4HI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
FokI GGATG 3 cut(s) 212, 670, 845
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
FspBI CTAG 2 cut(s) 549, 1022
GlaI GCGC 3 cut(s) 73, 154, 418
GluI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
GsaI CCCAGC 3 cut(s) 431, 657, 1020
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 2 cut(s) 715, 733
HapII CCGG 3 cut(s) 349, 730, 1003
HgaI GACGC 1 cut(s) 314
HhaI GCGC 3 cut(s) 74, 155, 419
Hin1I GRCGYC 2 cut(s) 306, 342
Hin1II CATG 6 cut(s) 72, 103, 947, 1101, 1142, 1285
Hin6I GCGC 3 cut(s) 72, 153, 417
HinP1I GCGC 3 cut(s) 72, 153, 417
HincII GTYRAC 4 cut(s) 340, 346, 411, 931
HindII GTYRAC 4 cut(s) 340, 346, 411, 931
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 4 cut(s) 520, 676, 908, 1202
HpaII CCGG 3 cut(s) 349, 730, 1003
HphI GGTGA 7 cut(s) 163, 466, 514, 643, 787, 1004, 1379
Hpy166II GTNNAC 7 cut(s) 181, 340, 346, 411, 931, 1091, 1370
Hpy188I TCNGA 3 cut(s) 148, 214, 1263
Hpy188III TCNNGA 3 cut(s) 322, 586, 1196
Hpy8I GTNNAC 7 cut(s) 181, 340, 346, 411, 931, 1091, 1370
HpyAV CCTTC 7 cut(s) 97, 178, 208, 247, 483, 490, 811
HpyCH4III ACNGT 3 cut(s) 178, 920, 1161
HpyCH4IV ACGT 4 cut(s) 342, 850, 1087, 1093
HpyCH4V TGCA 7 cut(s) 861, 951, 1085, 1108, 1129, 1142, 1299
HpyF3I CTNAG 3 cut(s) 211, 1026, 1212
HpySE526I ACGT 4 cut(s) 342, 850, 1087, 1093
Hsp92I GRCGYC 2 cut(s) 306, 342
Hsp92II CATG 6 cut(s) 72, 103, 947, 1101, 1142, 1285
HspAI GCGC 3 cut(s) 72, 153, 417
Kzo9I GATC 3 cut(s) 354, 984, 1258
LguI GCTCTTC 2 cut(s) 632, 1312
LmnI GCTCC 6 cut(s) 43, 79, 101, 275, 471, 757
Lsp1109I GCAGC 4 cut(s) 369, 963, 1069, 1334
LweI GCATC 2 cut(s) 988, 1352
MaeI CTAG 2 cut(s) 549, 1022
MaeII ACGT 4 cut(s) 342, 850, 1087, 1093
MaeIII GTNAC 3 cut(s) 482, 502, 965
MalI GATC 3 cut(s) 356, 986, 1260
MbiI CCGCTC 3 cut(s) 270, 474, 813
MboI GATC 3 cut(s) 354, 984, 1258
MboII GAAGA 7 cut(s) 601, 649, 1007, 1071, 1197, 1316, 1329
MhlI GDGCHC 2 cut(s) 644, 762
MlsI TGGCCA 1 cut(s) 715
MluCI AATT 4 cut(s) 397, 840, 1007, 1062
MluNI TGGCCA 1 cut(s) 715
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 171
Mox20I TGGCCA 1 cut(s) 715
MscI TGGCCA 1 cut(s) 715
MseI TTAA 1 cut(s) 672
MslI CAYNNNNRTG 1 cut(s) 1096
Msp20I TGGCCA 1 cut(s) 715
MspA1I CMGCKG 1 cut(s) 597
MspI CCGG 3 cut(s) 349, 730, 1003
MspR9I CCNGG 2 cut(s) 730, 1003
NciI CCSGG 2 cut(s) 730, 1003
NdeI CATATG 1 cut(s) 868
NdeII GATC 3 cut(s) 354, 984, 1258
NheI GCTAGC 1 cut(s) 1021
NlaIII CATG 6 cut(s) 72, 103, 947, 1101, 1142, 1285
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 3 cut(s) 947, 1142, 1285
PaeI GCATGC 1 cut(s) 1285
PaeR7I CTCGAG 1 cut(s) 1230
PciI ACATGT 1 cut(s) 943
PciSI GCTCTTC 2 cut(s) 632, 1312
PcsI WCGNNNNNNNCGW 5 cut(s) 38, 124, 312, 336, 399
PfeI GAWTC 3 cut(s) 676, 908, 1202
PflFI GACNNNGTC 1 cut(s) 935
PkrI GCNGC 8 cut(s) 301, 310, 371, 384, 953, 1075, 1084, 1324
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
Ppu21I YACGTR 1 cut(s) 1088
PscI ACATGT 1 cut(s) 943
PshAI GACNNNNGTC 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 644
PspFI CCCAGC 3 cut(s) 427, 653, 1016
PspPI GGNCC 1 cut(s) 732
PspXI VCTCGAGB 1 cut(s) 1230
PstNI CAGNNNCTG 1 cut(s) 218
PsyI GACNNNGTC 1 cut(s) 935
RsaI GTAC 3 cut(s) 876, 917, 1090
RsaNI GTAC 3 cut(s) 875, 916, 1089
RseI CAYNNNNRTG 1 cut(s) 1096
SacI GAGCTC 1 cut(s) 644
SalI GTCGAC 2 cut(s) 338, 344
SapI GCTCTTC 2 cut(s) 632, 1312
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 8 cut(s) 300, 309, 370, 383, 952, 1074, 1083, 1323
Sau3AI GATC 3 cut(s) 354, 984, 1258
Sau96I GGNCC 1 cut(s) 732
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 2 cut(s) 730, 1003
SduI GDGCHC 2 cut(s) 644, 762
SfaNI GCATC 2 cut(s) 988, 1352
Sfr274I CTCGAG 1 cut(s) 1230
SgrDI CGTCGACG 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 1230
SmiMI CAYNNNNRTG 1 cut(s) 1096
SmlI CTYRAG 2 cut(s) 1040, 1230
SmoI CTYRAG 2 cut(s) 1040, 1230
SphI GCATGC 1 cut(s) 1285
Sse9I AATT 4 cut(s) 397, 840, 1007, 1062
SspMI CTAG 2 cut(s) 549, 1022
SstI GAGCTC 1 cut(s) 644
StyD4I CCNGG 2 cut(s) 728, 1001
TaaI ACNGT 3 cut(s) 178, 920, 1161
TaiI ACGT 4 cut(s) 345, 853, 1090, 1096
TaqI TCGA 6 cut(s) 118, 240, 339, 345, 987, 1231
TasI AATT 4 cut(s) 397, 840, 1007, 1062
TauI GCSGC 4 cut(s) 302, 311, 372, 1076
TfiI GAWTC 3 cut(s) 676, 908, 1202
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TscAI CASTG 2 cut(s) 1306, 1338
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 4 cut(s) 382, 951, 1082, 1322
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 5 cut(s) 17, 833, 900, 1000, 1086
TspGWI ACGGA 3 cut(s) 30, 325, 501
TspRI CASTG 2 cut(s) 1306, 1338
Tth111I GACNNNGTC 1 cut(s) 935
XceI RCATGY 3 cut(s) 947, 1142, 1285
XhoI CTCGAG 1 cut(s) 1230
XmiI GTMKAC 2 cut(s) 339, 345
XspI CTAG 2 cut(s) 549, 1022
ZraI GACGTC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.