Rw4G011830

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005704
Physical Location & Seq
Reverse (-)
27969711 .. 27973481
3771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011830.1

Sequence Viewer

Length: 1395 bp
ATGAATTCCTCCGCCACGGCCACGGCCACGGCCACCGCCCACTCGCTCGCTCCGTCGCCATTCCACCGCTTCCGCAACCCATGCGCTCCCAAAGCCTCCTTCTCCTGCTCCATGCCTCGCCCTCCCAATCGAAACCGCCGAAACCGCAGACGAAACTCCGACAGCGCCACCACCGCCACCTTCACCACCGTCCACACCGCCAAGCCCGCCTTCTACACTTCCTCAGACCCTGAAAACATCCAAGCCACCTTCGACCTCAACAGCCTCTACCACTCCTCCCGCTCCTACCTCCGCCACTTCCTCTCCTCCGCCGCCGACGCCGCCGACGACCTCCAGACCCTCGTCTCCGTCGACGTCGACCGCAGGATCGTCGTCTCCTGCCGCCCCTCCACGCTGCGGTTCGTCGGCAATTTCGTCGTGTTGACCTGCGCCGTCGTGCTGGGGTTTAGGGTTCTGGTTGGGCTGGTGAGGTTAGGGTTTGGGAGCGGGTCGGGTTACGGAAGGGAGAAGGTGGTGACGAGGAGGGATAGGAGTCTTGGGGGGAAGGAGGTTGTGGTAGCTAGGATAGAGAAGCCAAGAGCAGAGGAGGTTAGTGTCAAGAAGAACAGCGGTTTGGGGATTTTGGATAATCCCCTATCGTCGGTGAGGAAGAGCTCAATGGTGGCTGGGATGGAGAGAGTGTTTAAGAATCGGGTTCGCTTCGGGGAGAAGCTTCCCCAATGGTGGCCAACTACAACTTCCCAGCCCATTTTAGGGGTTGGTAATGAGGAGCACCAGAGAGAAGCAAACAGATTGGTTAGAGCAATCACCGACAATAGAATGAGCGGGAAGGACATTATGGAGGATGATATAATTCATTTACGTCAAATATGCAGGACATATGGAGTACGGGTCTCCTTTGATACTACAAATACACGGGATTCATTGTACCGTGTGTCTGTTGACTTTGTCTTACATGTATGCAGCAGGACACCGAGTTACTCTAAAGGTGTTGAGATCGAGGGTGAAGATGCCCGGCAATTCATAGCTGGGCTAGCTGAGAACATTGGGCTTGAGAATGTTCGTGCTGGAAGAATTGTGTCTGCCGCCGTAGCTGCACGTACACGTTCATGTTTTTTGCAGGCTTGGGCTTTAGTAATGCAAGCTAAACATGCAGAAGCCGTAGTAGAACTGTCAAAAATATGCCTTGTTTGGCGGATTTTCCCTCCCGAGGAATCTTCGCCTGAGATGGAGATGGTGGCTCGAGGCTTGGAGAAACACTTGAAAAGGGATCAGAGGAGATTTCTTATGAGCATGCTTGTTGGCACTTGCAGTGAAGAGAGCCAGAGAAGAGCAGCAGAAGCACTGGGTTTGGCATCATCTTTCAAAGGTGTTGGTGGTGAACAATATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

464

Amino Acids

51.48

Weight (kDa)

9.82

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 434
AccBSI CCGCTC 3 cut(s) 282, 486, 825
AccI GTMKAC 2 cut(s) 351, 357
AclWI GGATC 2 cut(s) 374, 1278
AcoI YGGCCR 4 cut(s) 18, 24, 30, 725
AcsI RAATTY 1 cut(s) 4
AcyI GRCGYC 2 cut(s) 318, 354
AfaI GTAC 3 cut(s) 888, 929, 1102
AfiI CCNNNNNNNGG 6 cut(s) 396, 640, 723, 752, 753, 1210
AflIII ACRYGT 2 cut(s) 955, 1103
AgsI TTSAA 2 cut(s) 1264, 1366
AjuI GAANNNNNNNTTGG 8 cut(s) 83, 115, 194, 226, 596, 628, 721, 753
AluBI AGCT 7 cut(s) 560, 654, 712, 1028, 1037, 1094, 1145
AluI AGCT 7 cut(s) 560, 654, 712, 1028, 1037, 1094, 1145
Alw21I GWGCWC 2 cut(s) 656, 774
Alw26I GTCTC 3 cut(s) 349, 379, 898
AlwI GGATC 2 cut(s) 374, 1278
AlwNI CAGNNNCTG 1 cut(s) 230
Ama87I CYCGRG 2 cut(s) 1208, 1242
AoxI GGCC 4 cut(s) 18, 24, 30, 725
ApeKI GCWGC 4 cut(s) 394, 963, 1094, 1334
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 3 cut(s) 86, 167, 431
AsuC2I CCSGG 1 cut(s) 1015
AsuHPI GGTGA 7 cut(s) 175, 478, 526, 655, 799, 1016, 1391
AsuNHI GCTAGC 1 cut(s) 1033
AvaI CYCGRG 2 cut(s) 1208, 1242
BalI TGGCCA 1 cut(s) 727
BanII GRGCYC 1 cut(s) 656
Bbv12I GWGCWC 2 cut(s) 656, 774
BbvI GCAGC 4 cut(s) 381, 975, 1081, 1346
BccI CCATC 3 cut(s) 664, 1222, 1228
BceAI ACGGC 6 cut(s) 33, 39, 45, 416, 1073, 1145
BcgI CGANNNNNNTGC 4 cut(s) 352, 386, 397, 431
BcnI CCSGG 1 cut(s) 1015
BcoDI GTCTC 3 cut(s) 349, 379, 898
BfaI CTAG 2 cut(s) 561, 1034
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 8 cut(s) 312, 321, 382, 395, 964, 1086, 1095, 1335
BlsI GCNGC 8 cut(s) 313, 322, 383, 396, 965, 1087, 1096, 1336
Bme1390I CCNGG 1 cut(s) 1015
BmeT110I CYCGRG 2 cut(s) 1208, 1242
BmrFI CCNGG 1 cut(s) 1015
BmrI ACTGGG 1 cut(s) 1355
BmsI GCATC 2 cut(s) 1000, 1364
BmtI GCTAGC 1 cut(s) 1037
BmuI ACTGGG 1 cut(s) 1355
BoxI GACNNNNGTC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 317
BpuEI CTTGAG 1 cut(s) 1073
BpuMI CCSGG 1 cut(s) 1015
BsaAI YACGTR 1 cut(s) 1100
BsaHI GRCGYC 2 cut(s) 318, 354
BsaI GGTCTC 1 cut(s) 898
BsaJI CCNNGG 4 cut(s) 15, 21, 27, 1209
BsaXI ACNNNNNCTCC 8 cut(s) 260, 287, 290, 317, 665, 695, 876, 906
Bsc4I CCNNNNNNNGG 6 cut(s) 396, 640, 723, 752, 753, 1210
Bse1I ACTGG 1 cut(s) 1350
BseDI CCNNGG 4 cut(s) 15, 21, 27, 1209
BseGI GGATG 3 cut(s) 237, 675, 850
BseLI CCNNNNNNNGG 6 cut(s) 396, 640, 723, 752, 753, 1210
BseMII CTCAG 3 cut(s) 237, 1029, 1215
BseNI ACTGG 1 cut(s) 1350
BseRI GAGGAG 6 cut(s) 265, 295, 535, 599, 782, 1291
BseXI GCAGC 4 cut(s) 381, 975, 1081, 1346
BseYI CCCAGC 4 cut(s) 439, 665, 741, 1028
BsgI GTGCAG 1 cut(s) 1080
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 4 cut(s) 20, 26, 32, 727
BsiEI CGRYCG 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 656, 774
BsiHKCI CYCGRG 2 cut(s) 1208, 1242
BsiSI CCGG 1 cut(s) 1015
BslI CCNNNNNNNGG 6 cut(s) 396, 640, 723, 752, 753, 1210
BsmAI GTCTC 3 cut(s) 349, 379, 898
BsmBI CGTCTC 2 cut(s) 349, 379
BsnI GGCC 4 cut(s) 20, 26, 32, 727
Bso31I GGTCTC 1 cut(s) 898
BsoBI CYCGRG 2 cut(s) 1208, 1242
Bsp1286I GDGCHC 2 cut(s) 656, 774
Bsp143I GATC 3 cut(s) 366, 996, 1270
BspANI GGCC 4 cut(s) 20, 26, 32, 727
BspCNI CTCAG 3 cut(s) 236, 1030, 1216
BspMI ACCTGC 1 cut(s) 434
BspOI GCTAGC 1 cut(s) 1037
BspPI GGATC 2 cut(s) 374, 1278
BspQI GCTCTTC 2 cut(s) 644, 1324
BspTNI GGTCTC 1 cut(s) 898
BsrBI CCGCTC 3 cut(s) 282, 486, 825
BsrI ACTGG 1 cut(s) 1350
BssECI CCNNGG 4 cut(s) 15, 21, 27, 1209
BssMI GATC 3 cut(s) 366, 996, 1270
BssNI GRCGYC 2 cut(s) 318, 354
Bst4CI ACNGT 3 cut(s) 190, 932, 1173
Bst6I CTCTTC 3 cut(s) 644, 1311, 1324
BstACI GRCGYC 2 cut(s) 318, 354
BstAPI GCANNNNNTGC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 1100
BstC8I GCNNGC 6 cut(s) 48, 207, 1035, 1122, 1143, 1295
BstDEI CTNAG 3 cut(s) 223, 1038, 1224
BstDSI CCRYGG 3 cut(s) 15, 21, 27
BstF5I GGATG 3 cut(s) 237, 675, 850
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 86, 167, 431
BstKTI GATC 3 cut(s) 369, 999, 1273
BstMAI GTCTC 3 cut(s) 349, 379, 898
BstMBI GATC 3 cut(s) 366, 996, 1270
BstMCI CGRYCG 1 cut(s) 361
BstNSI RCATGY 3 cut(s) 959, 1154, 1297
BstPAI GACNNNNGTC 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 1013
BstV1I GCAGC 4 cut(s) 381, 975, 1081, 1346
BsuRI GGCC 4 cut(s) 20, 26, 32, 727
BtgI CCRYGG 3 cut(s) 15, 21, 27
BtsCI GGATG 3 cut(s) 237, 675, 850
BtsI GCAGTG 1 cut(s) 1318
BtsIMutI CAGTG 2 cut(s) 1318, 1343
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 6 cut(s) 48, 207, 1035, 1122, 1143, 1295
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 3 cut(s) 86, 167, 431
CseI GACGC 1 cut(s) 326
Csp6I GTAC 3 cut(s) 887, 928, 1101
CviAII CATG 6 cut(s) 81, 112, 956, 1110, 1151, 1294
CviQI GTAC 3 cut(s) 887, 928, 1101
DdeI CTNAG 3 cut(s) 223, 1038, 1224
DpnI GATC 3 cut(s) 368, 998, 1272
DpnII GATC 3 cut(s) 366, 996, 1270
EaeI YGGCCR 4 cut(s) 18, 24, 30, 725
Eam1104I CTCTTC 3 cut(s) 644, 1311, 1324
EarI CTCTTC 3 cut(s) 644, 1311, 1324
EciI GGCGGA 3 cut(s) 281, 298, 1210
Ecl136II GAGCTC 1 cut(s) 654
Eco24I GRGCYC 1 cut(s) 656
Eco31I GGTCTC 1 cut(s) 898
Eco53kI GAGCTC 1 cut(s) 654
Eco88I CYCGRG 2 cut(s) 1208, 1242
EcoICRI GAGCTC 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 4
EcoT38I GRGCYC 1 cut(s) 656
Esp3I CGTCTC 2 cut(s) 349, 379
FaeI CATG 6 cut(s) 84, 115, 959, 1113, 1154, 1297
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FatI CATG 6 cut(s) 80, 111, 955, 1109, 1150, 1293
FauI CCCGC 4 cut(s) 214, 287, 479, 818
FauNDI CATATG 1 cut(s) 880
FblI GTMKAC 2 cut(s) 351, 357
Fnu4HI GCNGC 8 cut(s) 312, 321, 382, 395, 964, 1086, 1095, 1335
FokI GGATG 3 cut(s) 224, 682, 857
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 8 cut(s) 312, 321, 382, 395, 964, 1086, 1095, 1335
FspBI CTAG 2 cut(s) 561, 1034
GlaI GCGC 3 cut(s) 85, 166, 430
GluI GCNGC 8 cut(s) 312, 321, 382, 395, 964, 1086, 1095, 1335
GsaI CCCAGC 4 cut(s) 443, 669, 745, 1032
GsuI CTGGAG 1 cut(s) 317
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 4 cut(s) 20, 26, 32, 727
HapII CCGG 1 cut(s) 1015
HgaI GACGC 1 cut(s) 326
HhaI GCGC 3 cut(s) 86, 167, 431
Hin1I GRCGYC 2 cut(s) 318, 354
Hin1II CATG 6 cut(s) 84, 115, 959, 1113, 1154, 1297
Hin6I GCGC 3 cut(s) 84, 165, 429
HinP1I GCGC 3 cut(s) 84, 165, 429
HincII GTYRAC 4 cut(s) 352, 358, 423, 943
HindII GTYRAC 4 cut(s) 352, 358, 423, 943
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 4 cut(s) 532, 688, 920, 1214
HpaII CCGG 1 cut(s) 1015
HphI GGTGA 7 cut(s) 175, 478, 526, 655, 799, 1016, 1391
Hpy166II GTNNAC 7 cut(s) 193, 352, 358, 423, 943, 1103, 1382
Hpy188I TCNGA 3 cut(s) 160, 226, 1275
Hpy188III TCNNGA 3 cut(s) 334, 598, 1208
Hpy8I GTNNAC 7 cut(s) 193, 352, 358, 423, 943, 1103, 1382
HpyAV CCTTC 8 cut(s) 109, 190, 220, 259, 495, 502, 538, 823
HpyCH4III ACNGT 3 cut(s) 190, 932, 1173
HpyCH4IV ACGT 4 cut(s) 354, 862, 1099, 1105
HpyCH4V TGCA 7 cut(s) 873, 963, 1097, 1120, 1141, 1154, 1311
HpyF3I CTNAG 3 cut(s) 223, 1038, 1224
HpySE526I ACGT 4 cut(s) 354, 862, 1099, 1105
Hsp92I GRCGYC 2 cut(s) 318, 354
Hsp92II CATG 6 cut(s) 84, 115, 959, 1113, 1154, 1297
HspAI GCGC 3 cut(s) 84, 165, 429
Kzo9I GATC 3 cut(s) 366, 996, 1270
LguI GCTCTTC 2 cut(s) 644, 1324
LmnI GCTCC 6 cut(s) 55, 91, 113, 287, 483, 769
Lsp1109I GCAGC 4 cut(s) 381, 975, 1081, 1346
LweI GCATC 2 cut(s) 1000, 1364
MaeI CTAG 2 cut(s) 561, 1034
MaeII ACGT 4 cut(s) 354, 862, 1099, 1105
MaeIII GTNAC 3 cut(s) 494, 514, 977
MalI GATC 3 cut(s) 368, 998, 1272
MbiI CCGCTC 3 cut(s) 282, 486, 825
MboI GATC 3 cut(s) 366, 996, 1270
MboII GAAGA 7 cut(s) 613, 661, 1019, 1083, 1209, 1328, 1341
MhlI GDGCHC 2 cut(s) 656, 774
MlsI TGGCCA 1 cut(s) 727
MluCI AATT 5 cut(s) 4, 409, 852, 1019, 1074
MluNI TGGCCA 1 cut(s) 727
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 727
MscI TGGCCA 1 cut(s) 727
MseI TTAA 1 cut(s) 684
MslI CAYNNNNRTG 1 cut(s) 1108
Msp20I TGGCCA 1 cut(s) 727
MspA1I CMGCKG 1 cut(s) 609
MspI CCGG 1 cut(s) 1015
MspR9I CCNGG 1 cut(s) 1015
NciI CCSGG 1 cut(s) 1015
NdeI CATATG 1 cut(s) 880
NdeII GATC 3 cut(s) 366, 996, 1270
NheI GCTAGC 1 cut(s) 1033
NlaIII CATG 6 cut(s) 84, 115, 959, 1113, 1154, 1297
NmuCI GTSAC 1 cut(s) 514
NspI RCATGY 3 cut(s) 959, 1154, 1297
PaeI GCATGC 1 cut(s) 1297
PaeR7I CTCGAG 1 cut(s) 1242
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 2 cut(s) 644, 1324
PcsI WCGNNNNNNNCGW 5 cut(s) 50, 136, 324, 348, 411
PfeI GAWTC 3 cut(s) 688, 920, 1214
PflFI GACNNNGTC 1 cut(s) 947
PkrI GCNGC 8 cut(s) 313, 322, 383, 396, 965, 1087, 1096, 1336
PleI GAGTC 1 cut(s) 540
PpsI GAGTC 1 cut(s) 540
Ppu21I YACGTR 1 cut(s) 1100
PscI ACATGT 1 cut(s) 955
PshAI GACNNNNGTC 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 656
PspFI CCCAGC 4 cut(s) 439, 665, 741, 1028
PspXI VCTCGAGB 1 cut(s) 1242
PstNI CAGNNNCTG 1 cut(s) 230
PsyI GACNNNGTC 1 cut(s) 947
RsaI GTAC 3 cut(s) 888, 929, 1102
RsaNI GTAC 3 cut(s) 887, 928, 1101
RseI CAYNNNNRTG 1 cut(s) 1108
SacI GAGCTC 1 cut(s) 656
SalI GTCGAC 2 cut(s) 350, 356
SapI GCTCTTC 2 cut(s) 644, 1324
SaqAI TTAA 1 cut(s) 684
SatI GCNGC 8 cut(s) 312, 321, 382, 395, 964, 1086, 1095, 1335
Sau3AI GATC 3 cut(s) 366, 996, 1270
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 1 cut(s) 1015
SduI GDGCHC 2 cut(s) 656, 774
SfaNI GCATC 2 cut(s) 1000, 1364
Sfr274I CTCGAG 1 cut(s) 1242
SgrDI CGTCGACG 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 1242
SmiMI CAYNNNNRTG 1 cut(s) 1108
SmlI CTYRAG 2 cut(s) 1052, 1242
SmoI CTYRAG 2 cut(s) 1052, 1242
SphI GCATGC 1 cut(s) 1297
Sse9I AATT 5 cut(s) 4, 409, 852, 1019, 1074
SspMI CTAG 2 cut(s) 561, 1034
SstI GAGCTC 1 cut(s) 656
StyD4I CCNGG 1 cut(s) 1013
TaaI ACNGT 3 cut(s) 190, 932, 1173
TaiI ACGT 4 cut(s) 357, 865, 1102, 1108
TaqI TCGA 6 cut(s) 130, 252, 351, 357, 999, 1243
TasI AATT 5 cut(s) 4, 409, 852, 1019, 1074
TauI GCSGC 4 cut(s) 314, 323, 384, 1088
TfiI GAWTC 3 cut(s) 688, 920, 1214
Tru1I TTAA 1 cut(s) 684
Tru9I TTAA 1 cut(s) 684
TscAI CASTG 2 cut(s) 1318, 1350
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 4 cut(s) 394, 963, 1094, 1334
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 5 cut(s) 17, 845, 912, 1012, 1098
TspGWI ACGGA 3 cut(s) 42, 337, 513
TspRI CASTG 2 cut(s) 1318, 1350
Tth111I GACNNNGTC 1 cut(s) 947
XapI RAATTY 1 cut(s) 4
XceI RCATGY 3 cut(s) 959, 1154, 1297
XhoI CTCGAG 1 cut(s) 1242
XmiI GTMKAC 2 cut(s) 351, 357
XspI CTAG 2 cut(s) 561, 1034
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.