RchiOBHm_Chr4g0407651

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005704
Physical Location & Seq
Reverse (-)
30002106 .. 30006231
4126 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37887

Sequence Viewer

Length: 1389 bp
ATGAACTCCTCCGCCACCGCCACCGCCACCGCCCACTCGCTCGCTCCGTCGCCATTACACCGCTTCCGCAACCCATGCGCTCCCAAAGCCTCCTTCTCCTGCTCCATGCCTCGCCCTCCCAATCGAAACCGCCGAAACCGCAGACGAAACTCCGACAGCGCCACCACCGCCACCTTCACCACCGTCCCCACCGCCAAGCCCGCCTTCTACACTTCCTCAGACCCTGAAAACATCCAAGCCACCTTCGACCTCAACAGCCTCTACCACTCCTCCCACTCCTACCTCCGCCACTTCCTCTCCTCCGCCGCCGACGCCGCCGCCGACCTCCAGACTCTCGTCTCCGTCGACGTCGACCGCAGGATCGTCGTCTCCTGCCGCCCCTCCACGCTGCGGTTCGTCGGCAATTTCGTCGTGTTGACCTGCGCCGTCGTGCTGGGGTTTAGGGTTCTGGTTGGGTTGGTGAGGTTAGGGTTTGGGAGCGGGTCGGGTTACGGAAGGGAGAAGGTGGTGACGAGGAGGGATAGGAGTCTTGGGGGGAAGGAGGTTGTGGTAGCTAGGATAGAGAAGCCGAGAGCAGAGGAGGTTAGTGTCAAGAAGAACAGCGGTTTGGGGATTTTGGATAATCCCCTATCGTCGGTGAGGAAGAGCTCAATGGTGGCTGGGATGGAGAGAGTGTTTAAGAATCGGGTTCGCTTCGGGGAGAAGCTTCCCCAATGGTGGCCAACTACAACTTCCCAGCCCATTTTAGGGGTTGATAATGAGGAGCACCAGAGAGAAGCAAATAGATTGGTTAGAGCAATCACCGACAGTAGAATGAGCGGGAAGGACATTATGGAGGATGATATAATTCATTTACGTCAAATATGCAGGACATATGGAGTACGGGTCTCCTTTGATACTACAAATACACGAGATTCATTGTACCGTGTGTCTGTTGACTTTGTCTTCAATGTATGCAGCAGGACACCGAGTTACTCTAAAGGTGTTGAGATCGAGGGTGAAGATGCCCGGCAATTCATAGCTGGGCTAGCTGAGAACATTGGGCTTGAGAATGTTCGTGCTGGAAGAATTGTGTCTGCCGCCGTAGCTGCGCGTACACGTTCATGTTTTTTGCAGGCTTGGGCTTTAGTAATGCAAGCTAAACATGCAGAAGCCGTAGTAGAACTGTCAAAGATATGCCTTGTTTGGCGGATTTTCCCTCCCGAGGAATCTTCGCCTGAGATGGAGATGGTGGCTCGAGGCTTGGAGAAACACTTGAAAAGGGATCAGAGGAGATTTCTTATGAGCATGCTTGTTGGCACTTGCAGTGAAGAGAGCCAGAGAAGAGCAGCAGAAGCACTGGGTTTGGCATCATCTTTCAAAGGTGTTGGTGATGAACAATATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

51.27

Weight (kDa)

9.69

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
Acc36I ACCTGC 1 cut(s) 428
AccBSI CCGCTC 2 cut(s) 480, 819
AccI GTMKAC 2 cut(s) 345, 351
AccII CGCG 1 cut(s) 1093
AclWI GGATC 2 cut(s) 368, 1272
AcoI YGGCCR 1 cut(s) 719
AcyI GRCGYC 2 cut(s) 312, 348
AfaI GTAC 3 cut(s) 882, 923, 1096
AfiI CCNNNNNNNGG 6 cut(s) 390, 634, 717, 746, 747, 1204
AflIII ACRYGT 1 cut(s) 1097
AgsI TTSAA 3 cut(s) 949, 1258, 1360
AjuI GAANNNNNNNTTGG 8 cut(s) 77, 109, 188, 220, 590, 622, 715, 747
AluBI AGCT 7 cut(s) 554, 648, 706, 1022, 1031, 1088, 1139
AluI AGCT 7 cut(s) 554, 648, 706, 1022, 1031, 1088, 1139
Alw21I GWGCWC 2 cut(s) 650, 768
Alw26I GTCTC 3 cut(s) 343, 373, 892
AlwI GGATC 2 cut(s) 368, 1272
AlwNI CAGNNNCTG 1 cut(s) 224
Ama87I CYCGRG 2 cut(s) 1202, 1236
AoxI GGCC 1 cut(s) 719
ApeKI GCWGC 4 cut(s) 388, 957, 1088, 1328
AspLEI GCGC 4 cut(s) 80, 161, 425, 1093
AsuC2I CCSGG 1 cut(s) 1009
AsuHPI GGTGA 7 cut(s) 169, 472, 520, 649, 793, 1010, 1382
AsuNHI GCTAGC 1 cut(s) 1027
AvaI CYCGRG 2 cut(s) 1202, 1236
BalI TGGCCA 1 cut(s) 721
BanII GRGCYC 1 cut(s) 650
BauI CACGAG 1 cut(s) 909
BbsI GAAGAC 1 cut(s) 937
Bbv12I GWGCWC 2 cut(s) 650, 768
BbvI GCAGC 4 cut(s) 375, 969, 1075, 1340
BccI CCATC 3 cut(s) 658, 1216, 1222
BceAI ACGGC 3 cut(s) 410, 1067, 1139
BcgI CGANNNNNNTGC 4 cut(s) 346, 380, 391, 425
BcnI CCSGG 1 cut(s) 1009
BcoDI GTCTC 3 cut(s) 343, 373, 892
BfaI CTAG 2 cut(s) 555, 1028
BfoI RGCGCY 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 428
BisI GCNGC 9 cut(s) 306, 315, 318, 376, 389, 958, 1080, 1089, 1329
BlsI GCNGC 9 cut(s) 307, 316, 319, 377, 390, 959, 1081, 1090, 1330
Bme1390I CCNGG 1 cut(s) 1009
BmeT110I CYCGRG 2 cut(s) 1202, 1236
BmrFI CCNGG 1 cut(s) 1009
BmrI ACTGGG 1 cut(s) 1349
BmsI GCATC 2 cut(s) 994, 1358
BmtI GCTAGC 1 cut(s) 1031
BmuI ACTGGG 1 cut(s) 1349
BoxI GACNNNNGTC 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 937
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 1067
BpuMI CCSGG 1 cut(s) 1009
BsaHI GRCGYC 2 cut(s) 312, 348
BsaI GGTCTC 1 cut(s) 892
BsaJI CCNNGG 1 cut(s) 1203
BsaXI ACNNNNNCTCC 8 cut(s) 254, 281, 284, 311, 659, 689, 870, 900
Bsc4I CCNNNNNNNGG 6 cut(s) 390, 634, 717, 746, 747, 1204
Bse1I ACTGG 1 cut(s) 1344
BseDI CCNNGG 1 cut(s) 1203
BseGI GGATG 3 cut(s) 231, 669, 844
BseLI CCNNNNNNNGG 6 cut(s) 390, 634, 717, 746, 747, 1204
BseMII CTCAG 3 cut(s) 231, 1023, 1209
BseNI ACTGG 1 cut(s) 1344
BseRI GAGGAG 6 cut(s) 259, 289, 529, 593, 776, 1285
BseXI GCAGC 4 cut(s) 375, 969, 1075, 1340
BseYI CCCAGC 4 cut(s) 433, 659, 735, 1022
Bsh1236I CGCG 1 cut(s) 1093
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 1 cut(s) 721
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 2 cut(s) 650, 768
BsiHKCI CYCGRG 2 cut(s) 1202, 1236
BsiSI CCGG 1 cut(s) 1009
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 6 cut(s) 390, 634, 717, 746, 747, 1204
BsmAI GTCTC 3 cut(s) 343, 373, 892
BsmBI CGTCTC 2 cut(s) 343, 373
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 1 cut(s) 721
Bso31I GGTCTC 1 cut(s) 892
BsoBI CYCGRG 2 cut(s) 1202, 1236
Bsp1286I GDGCHC 2 cut(s) 650, 768
Bsp143I GATC 3 cut(s) 360, 990, 1264
BspANI GGCC 1 cut(s) 721
BspCNI CTCAG 3 cut(s) 230, 1024, 1210
BspFNI CGCG 1 cut(s) 1093
BspMI ACCTGC 1 cut(s) 428
BspOI GCTAGC 1 cut(s) 1031
BspPI GGATC 2 cut(s) 368, 1272
BspQI GCTCTTC 2 cut(s) 638, 1318
BspTNI GGTCTC 1 cut(s) 892
BsrBI CCGCTC 2 cut(s) 480, 819
BsrI ACTGG 1 cut(s) 1344
BssECI CCNNGG 1 cut(s) 1203
BssMI GATC 3 cut(s) 360, 990, 1264
BssNI GRCGYC 2 cut(s) 312, 348
BssSI CACGAG 1 cut(s) 909
Bst2BI CACGAG 1 cut(s) 909
Bst4CI ACNGT 4 cut(s) 184, 809, 926, 1167
Bst6I CTCTTC 3 cut(s) 638, 1305, 1318
BstACI GRCGYC 2 cut(s) 312, 348
BstAPI GCANNNNNTGC 1 cut(s) 75
BstC8I GCNNGC 6 cut(s) 42, 201, 1029, 1116, 1137, 1289
BstDEI CTNAG 3 cut(s) 217, 1032, 1218
BstF5I GGATG 3 cut(s) 231, 669, 844
BstFNI CGCG 1 cut(s) 1093
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 4 cut(s) 80, 161, 425, 1093
BstKTI GATC 3 cut(s) 363, 993, 1267
BstMAI GTCTC 3 cut(s) 343, 373, 892
BstMBI GATC 3 cut(s) 360, 990, 1264
BstMCI CGRYCG 1 cut(s) 355
BstNSI RCATGY 2 cut(s) 1148, 1291
BstPAI GACNNNNGTC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 1007
BstUI CGCG 1 cut(s) 1093
BstV1I GCAGC 4 cut(s) 375, 969, 1075, 1340
BstV2I GAAGAC 1 cut(s) 937
BsuRI GGCC 1 cut(s) 721
BtsCI GGATG 3 cut(s) 231, 669, 844
BtsI GCAGTG 1 cut(s) 1312
BtsIMutI CAGTG 2 cut(s) 1312, 1337
BveI ACCTGC 1 cut(s) 428
Cac8I GCNNGC 6 cut(s) 42, 201, 1029, 1116, 1137, 1289
CaiI CAGNNNCTG 1 cut(s) 224
CfoI GCGC 4 cut(s) 80, 161, 425, 1093
CseI GACGC 1 cut(s) 320
Csp6I GTAC 3 cut(s) 881, 922, 1095
CviAII CATG 5 cut(s) 75, 106, 1104, 1145, 1288
CviQI GTAC 3 cut(s) 881, 922, 1095
DdeI CTNAG 3 cut(s) 217, 1032, 1218
DpnI GATC 3 cut(s) 362, 992, 1266
DpnII GATC 3 cut(s) 360, 990, 1264
EaeI YGGCCR 1 cut(s) 719
Eam1104I CTCTTC 3 cut(s) 638, 1305, 1318
EarI CTCTTC 3 cut(s) 638, 1305, 1318
EciI GGCGGA 3 cut(s) 275, 292, 1204
Ecl136II GAGCTC 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 650
Eco31I GGTCTC 1 cut(s) 892
Eco53kI GAGCTC 1 cut(s) 648
Eco88I CYCGRG 2 cut(s) 1202, 1236
EcoICRI GAGCTC 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 650
Esp3I CGTCTC 2 cut(s) 343, 373
FaeI CATG 5 cut(s) 78, 109, 1107, 1148, 1291
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FaqI GGGAC 1 cut(s) 170
FatI CATG 5 cut(s) 74, 105, 1103, 1144, 1287
FauI CCCGC 3 cut(s) 208, 473, 812
FauNDI CATATG 1 cut(s) 874
FblI GTMKAC 2 cut(s) 345, 351
Fnu4HI GCNGC 9 cut(s) 306, 315, 318, 376, 389, 958, 1080, 1089, 1329
FokI GGATG 3 cut(s) 218, 676, 851
FriOI GRGCYC 1 cut(s) 650
Fsp4HI GCNGC 9 cut(s) 306, 315, 318, 376, 389, 958, 1080, 1089, 1329
FspBI CTAG 2 cut(s) 555, 1028
GlaI GCGC 4 cut(s) 79, 160, 424, 1092
GluI GCNGC 9 cut(s) 306, 315, 318, 376, 389, 958, 1080, 1089, 1329
GsaI CCCAGC 4 cut(s) 437, 663, 739, 1026
GsuI CTGGAG 1 cut(s) 311
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 1 cut(s) 721
HapII CCGG 1 cut(s) 1009
HgaI GACGC 1 cut(s) 320
HhaI GCGC 4 cut(s) 80, 161, 425, 1093
Hin1I GRCGYC 2 cut(s) 312, 348
Hin1II CATG 5 cut(s) 78, 109, 1107, 1148, 1291
Hin6I GCGC 4 cut(s) 78, 159, 423, 1091
HinP1I GCGC 4 cut(s) 78, 159, 423, 1091
HincII GTYRAC 4 cut(s) 346, 352, 417, 937
HindII GTYRAC 4 cut(s) 346, 352, 417, 937
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 5 cut(s) 331, 526, 682, 914, 1208
HpaII CCGG 1 cut(s) 1009
HphI GGTGA 7 cut(s) 169, 472, 520, 649, 793, 1010, 1382
Hpy166II GTNNAC 5 cut(s) 346, 352, 417, 937, 1097
Hpy188I TCNGA 3 cut(s) 154, 220, 1269
Hpy188III TCNNGA 3 cut(s) 328, 592, 1202
Hpy8I GTNNAC 5 cut(s) 346, 352, 417, 937, 1097
HpyAV CCTTC 8 cut(s) 103, 184, 214, 253, 489, 496, 532, 817
HpyCH4III ACNGT 4 cut(s) 184, 809, 926, 1167
HpyCH4IV ACGT 3 cut(s) 348, 856, 1099
HpyCH4V TGCA 6 cut(s) 867, 957, 1114, 1135, 1148, 1305
HpyF3I CTNAG 3 cut(s) 217, 1032, 1218
HpySE526I ACGT 3 cut(s) 348, 856, 1099
Hsp92I GRCGYC 2 cut(s) 312, 348
Hsp92II CATG 5 cut(s) 78, 109, 1107, 1148, 1291
HspAI GCGC 4 cut(s) 78, 159, 423, 1091
Kzo9I GATC 3 cut(s) 360, 990, 1264
LguI GCTCTTC 2 cut(s) 638, 1318
LmnI GCTCC 5 cut(s) 49, 85, 107, 477, 763
Lsp1109I GCAGC 4 cut(s) 375, 969, 1075, 1340
LweI GCATC 2 cut(s) 994, 1358
MaeI CTAG 2 cut(s) 555, 1028
MaeII ACGT 3 cut(s) 348, 856, 1099
MaeIII GTNAC 3 cut(s) 488, 508, 971
MalI GATC 3 cut(s) 362, 992, 1266
MbiI CCGCTC 2 cut(s) 480, 819
MboI GATC 3 cut(s) 360, 990, 1264
MboII GAAGA 8 cut(s) 607, 655, 937, 1013, 1077, 1203, 1322, 1335
MhlI GDGCHC 2 cut(s) 650, 768
MlsI TGGCCA 1 cut(s) 721
MluCI AATT 4 cut(s) 403, 846, 1013, 1068
MluNI TGGCCA 1 cut(s) 721
MlyI GAGTC 2 cut(s) 325, 535
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 1 cut(s) 721
MscI TGGCCA 1 cut(s) 721
MseI TTAA 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 1102
Msp20I TGGCCA 1 cut(s) 721
MspA1I CMGCKG 1 cut(s) 603
MspI CCGG 1 cut(s) 1009
MspR9I CCNGG 1 cut(s) 1009
MvnI CGCG 1 cut(s) 1093
NciI CCSGG 1 cut(s) 1009
NdeI CATATG 1 cut(s) 874
NdeII GATC 3 cut(s) 360, 990, 1264
NheI GCTAGC 1 cut(s) 1027
NlaIII CATG 5 cut(s) 78, 109, 1107, 1148, 1291
NmeAIII GCCGAG 1 cut(s) 594
NmuCI GTSAC 1 cut(s) 508
NspI RCATGY 2 cut(s) 1148, 1291
PaeI GCATGC 1 cut(s) 1291
PaeR7I CTCGAG 1 cut(s) 1236
PciSI GCTCTTC 2 cut(s) 638, 1318
PcsI WCGNNNNNNNCGW 5 cut(s) 44, 130, 318, 342, 405
PfeI GAWTC 3 cut(s) 682, 914, 1208
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 9 cut(s) 307, 316, 319, 377, 390, 959, 1081, 1090, 1330
PleI GAGTC 2 cut(s) 325, 534
PpsI GAGTC 2 cut(s) 325, 534
PshAI GACNNNNGTC 1 cut(s) 335
Psp124BI GAGCTC 1 cut(s) 650
PspFI CCCAGC 4 cut(s) 433, 659, 735, 1022
PspXI VCTCGAGB 1 cut(s) 1236
PstNI CAGNNNCTG 1 cut(s) 224
PsyI GACNNNGTC 1 cut(s) 941
RsaI GTAC 3 cut(s) 882, 923, 1096
RsaNI GTAC 3 cut(s) 881, 922, 1095
RseI CAYNNNNRTG 1 cut(s) 1102
SacI GAGCTC 1 cut(s) 650
SalI GTCGAC 2 cut(s) 344, 350
SapI GCTCTTC 2 cut(s) 638, 1318
SaqAI TTAA 1 cut(s) 678
SatI GCNGC 9 cut(s) 306, 315, 318, 376, 389, 958, 1080, 1089, 1329
Sau3AI GATC 3 cut(s) 360, 990, 1264
SchI GAGTC 2 cut(s) 325, 535
ScrFI CCNGG 1 cut(s) 1009
SduI GDGCHC 2 cut(s) 650, 768
SfaNI GCATC 2 cut(s) 994, 1358
Sfr274I CTCGAG 1 cut(s) 1236
SgrDI CGTCGACG 1 cut(s) 344
SlaI CTCGAG 1 cut(s) 1236
SmiMI CAYNNNNRTG 1 cut(s) 1102
SmlI CTYRAG 2 cut(s) 1046, 1236
SmoI CTYRAG 2 cut(s) 1046, 1236
SphI GCATGC 1 cut(s) 1291
Sse9I AATT 4 cut(s) 403, 846, 1013, 1068
SspMI CTAG 2 cut(s) 555, 1028
SstI GAGCTC 1 cut(s) 650
StyD4I CCNGG 1 cut(s) 1007
TaaI ACNGT 4 cut(s) 184, 809, 926, 1167
TaiI ACGT 3 cut(s) 351, 859, 1102
TaqI TCGA 6 cut(s) 124, 246, 345, 351, 993, 1237
TasI AATT 4 cut(s) 403, 846, 1013, 1068
TauI GCSGC 5 cut(s) 308, 317, 320, 378, 1082
TfiI GAWTC 3 cut(s) 682, 914, 1208
Tru1I TTAA 1 cut(s) 678
Tru9I TTAA 1 cut(s) 678
TscAI CASTG 2 cut(s) 1312, 1344
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 4 cut(s) 388, 957, 1088, 1328
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 6 cut(s) 17, 839, 906, 1006, 1092, 1389
TspGWI ACGGA 3 cut(s) 36, 331, 507
TspRI CASTG 2 cut(s) 1312, 1344
Tth111I GACNNNGTC 1 cut(s) 941
XceI RCATGY 2 cut(s) 1148, 1291
XhoI CTCGAG 1 cut(s) 1236
XmiI GTMKAC 2 cut(s) 345, 351
XspI CTAG 2 cut(s) 555, 1028
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.