Rmu_sc0001743.1_g000005

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001743.1
OG0005704
Physical Location & Seq
Reverse (-)
11522 .. 15317
3796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001743.1_g000005.1.cds

Sequence Viewer

Length: 1365 bp
atggcatctaaactagagtttctcagcaggaaccaccggctaggtatacaagaccatcaaggggaagttgtgttaggtgctagtgagtcaagtggaccgcaggtggaggaagacggtggacaacctcaccaaggacaagacatgccagggatcaccgcacgacacaagacgcttgttgtggtgtcgatttgcttcagtgctttctccattttcgccttggatggatttgcgaggcatgaggggtggagatccggatcagatcagatctttggttttcgagagtttgggatccaatcacaatttctatgtgtttattggaattcgaaggttgaaaaaagaaagattgatagaatcacacctgatgatgttggtcggagtaagatgggaggggctgtggcttactatcgaagcactggactgaagaaagactacaacacatttgaggtcattccgattcaattccgtcagtttctaacgttaactgctgacgtggcattccaactcagcaaaagtgggacccacacgcatgctctgggactgtgggccgccgtagacggtgttgtcggggataaaacggctgcaatgaatgccatgagagagagagagagagagagagaccgcagacgaaactccaacagcaccaccaccaccaccaccgccaagcccgccttctacacttcttttgaccctgaaaacttccaagccaccttcaacctcaacagcctctaccactcctcccactcctacctctgccacttcctcgaagccgctgaagacctccagaccctcgtcttcgtcgacgttgaccgcaggatagtcgtctcctgccgcccctccacgctgcgattagtcggcaatttcgtcgtgttgacctgcaccgtcgtgctgggggagaaggtggtgacgaggagggataggattcttggagggaaagaggttgtggtagcgaggatagagaagccaagagcagaggaggttagaatcaagaagaacaggggtttggcgattttggataatcacctatcgtcggtgaggaagagctcaatggtggctgggatggagagagttcgcttcggggagaagcttcgaagaagaagaggagaggaggagagaggtccacaggatccggaactttcaagggtttcagttaccaaatgggaggagctgaaagagcctcatatgaagaacttgaggcaccatgtatcaattagtacggtaaggcagcacaacacaactaattctggcgcattcttcccacttcgctatgcaacaaagaaagtccaagttcgaagtggcttggttcatgagagcaagcttagtagactttgttgtccttggaagactgtgatccaagtggttttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

454

Amino Acids

51.34

Weight (kDa)

9.7

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 91
AasI GACNNNNNNGTC 2 cut(s) 560, 1329
Acc36I ACCTGC 2 cut(s) 91, 881
AccB1I GGYRCC 1 cut(s) 1194
AccI GTMKAC 4 cut(s) 46, 552, 798, 1321
AccIII TCCGGA 2 cut(s) 251, 1126
AciI CCGC 9 cut(s) 98, 156, 546, 619, 657, 666, 768, 808, 829
AclI AACGTT 1 cut(s) 476
AclWI GGATC 8 cut(s) 158, 243, 262, 283, 296, 1118, 1131, 1342
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 3 cut(s) 178, 440, 792
AfaI GTAC 1 cut(s) 1213
AfiI CCNNNNNNNGG 3 cut(s) 61, 131, 1027
AgsI TTSAA 4 cut(s) 332, 458, 712, 1137
AjiI CACGTC 1 cut(s) 490
AjnI CCWGG 1 cut(s) 145
AjuI GAANNNNNNNTTGG 6 cut(s) 285, 317, 653, 685, 983, 1015
AleI CACNNNNGTG 1 cut(s) 881
AluBI AGCT 4 cut(s) 1041, 1084, 1165, 1315
AluI AGCT 4 cut(s) 1041, 1084, 1165, 1315
Alw21I GWGCWC 1 cut(s) 1043
Alw26I GTCTC 2 cut(s) 609, 826
AlwI GGATC 8 cut(s) 158, 243, 262, 283, 296, 1118, 1131, 1342
Aor13HI TCCGGA 2 cut(s) 251, 1126
AoxI GGCC 1 cut(s) 543
ApeKI GCWGC 3 cut(s) 578, 841, 1222
ApoI RAATTY 1 cut(s) 319
AspLEI GCGC 1 cut(s) 1247
AspS9I GGNCC 4 cut(s) 95, 516, 543, 1115
AsuHPI GGTGA 5 cut(s) 119, 145, 913, 1010, 1042
AsuII TTCGAA 3 cut(s) 323, 1087, 1288
AvaII GGWCC 3 cut(s) 95, 516, 1115
BamHI GGATCC 2 cut(s) 288, 1123
BanI GGYRCC 1 cut(s) 1194
BanII GRGCYC 1 cut(s) 1043
BarI GAAGNNNNNNTAC 2 cut(s) 413, 445
BbsI GAAGAC 4 cut(s) 117, 780, 784, 1346
Bbv12I GWGCWC 1 cut(s) 1043
BbvI GCAGC 3 cut(s) 565, 828, 1234
BccI CCATC 4 cut(s) 63, 215, 376, 1051
BceAI ACGGC 2 cut(s) 533, 591
BcgI CGANNNNNNTGC 4 cut(s) 799, 833, 844, 878
BciT130I CCWGG 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 609, 826
BfaI CTAG 3 cut(s) 14, 41, 81
BfuAI ACCTGC 2 cut(s) 91, 881
BglII AGATCT 1 cut(s) 264
BisI GCNGC 6 cut(s) 546, 579, 768, 829, 842, 1223
BlsI GCNGC 6 cut(s) 547, 580, 769, 830, 843, 1224
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 3 cut(s) 95, 516, 1115
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 4 cut(s) 95, 516, 543, 1115
BmiI GGNNCC 6 cut(s) 32, 290, 517, 518, 1125, 1196
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 14
BoxI GACNNNNGTC 1 cut(s) 788
BpiI GAAGAC 4 cut(s) 117, 780, 784, 1346
BpmI CTGGAG 1 cut(s) 764
Bpu14I TTCGAA 3 cut(s) 323, 1087, 1288
BpuEI CTTGAG 1 cut(s) 1210
BsaBI GATNNNNATC 1 cut(s) 253
BsaI GGTCTC 1 cut(s) 609
BsaJI CCNNGG 4 cut(s) 130, 146, 216, 1334
BsaWI WCCGGW 2 cut(s) 251, 1126
BsaXI ACNNNNNCTCC 4 cut(s) 378, 408, 719, 749
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 131, 1027
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 588
Bse8I GATNNNNATC 1 cut(s) 253
BseAI TCCGGA 2 cut(s) 251, 1126
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 4 cut(s) 130, 146, 216, 1334
BseGI GGATG 2 cut(s) 226, 1062
BseJI GATNNNNATC 1 cut(s) 253
BseLI CCNNNNNNNGG 3 cut(s) 61, 131, 1027
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 2 cut(s) 37, 517
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 7 cut(s) 724, 922, 986, 1113, 1118, 1121, 1175
BseXI GCAGC 3 cut(s) 565, 828, 1234
BseYI CCCAGC 2 cut(s) 886, 1052
BsgI GTGCAG 1 cut(s) 859
BshFI GGCC 1 cut(s) 545
BshNI GGYRCC 1 cut(s) 1194
BsiHKAI GWGCWC 1 cut(s) 1043
BsiSI CCGG 3 cut(s) 37, 252, 1127
BslFI GGGAC 2 cut(s) 529, 549
BslI CCNNNNNNNGG 3 cut(s) 61, 131, 1027
BsmAI GTCTC 2 cut(s) 609, 826
BsmBI CGTCTC 1 cut(s) 826
BsmFI GGGAC 2 cut(s) 529, 549
BsmI GAATGC 3 cut(s) 494, 592, 1247
BsnI GGCC 1 cut(s) 545
Bso31I GGTCTC 1 cut(s) 609
Bsp119I TTCGAA 3 cut(s) 323, 1087, 1288
Bsp1286I GDGCHC 1 cut(s) 1043
Bsp13I TCCGGA 2 cut(s) 251, 1126
Bsp143I GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
BspACI CCGC 9 cut(s) 98, 156, 546, 619, 657, 666, 768, 808, 829
BspANI GGCC 1 cut(s) 545
BspCNI CTCAG 2 cut(s) 36, 516
BspEI TCCGGA 2 cut(s) 251, 1126
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 6 cut(s) 32, 290, 517, 518, 1125, 1196
BspMI ACCTGC 2 cut(s) 91, 881
BspPI GGATC 8 cut(s) 158, 243, 262, 283, 296, 1118, 1131, 1342
BspQI GCTCTTC 1 cut(s) 1031
BspT104I TTCGAA 3 cut(s) 323, 1087, 1288
BspT107I GGYRCC 1 cut(s) 1194
BspTNI GGTCTC 1 cut(s) 609
BsrDI GCAATG 1 cut(s) 588
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 1 cut(s) 418
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 4 cut(s) 130, 146, 216, 1334
BssMI GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
BssNAI GTATAC 1 cut(s) 47
BssT1I CCWWGG 3 cut(s) 130, 216, 1334
Bst1107I GTATAC 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 6 cut(s) 116, 540, 557, 880, 1216, 1345
Bst6I CTCTTC 2 cut(s) 1031, 1090
BstAPI GCANNNNNTGC 1 cut(s) 587
BstBI TTCGAA 3 cut(s) 323, 1087, 1288
BstC8I GCNNGC 3 cut(s) 528, 666, 1313
BstDEI CTNAG 3 cut(s) 23, 503, 1316
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 226, 1062
BstHHI GCGC 1 cut(s) 1247
BstKTI GATC 8 cut(s) 153, 251, 257, 262, 267, 291, 1126, 1350
BstMAI GTCTC 2 cut(s) 609, 826
BstMBI GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
BstMWI GCNNNNNNNGC 4 cut(s) 491, 587, 665, 1171
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 2 cut(s) 145, 530
BstPAI GACNNNNGTC 1 cut(s) 788
BstSCI CCNGG 1 cut(s) 145
BstV1I GCAGC 3 cut(s) 565, 828, 1234
BstV2I GAAGAC 4 cut(s) 117, 780, 784, 1346
BstX2I RGATCY 4 cut(s) 248, 264, 288, 1123
BstYI RGATCY 4 cut(s) 248, 264, 288, 1123
BstZ17I GTATAC 1 cut(s) 47
BsuRI GGCC 1 cut(s) 545
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 2 cut(s) 226, 1062
BtsIMutI CAGTG 2 cut(s) 202, 411
BveI ACCTGC 2 cut(s) 91, 881
Cac8I GCNNGC 3 cut(s) 528, 666, 1313
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 1 cut(s) 1247
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 4 cut(s) 95, 516, 543, 1115
CseI GACGC 1 cut(s) 178
Csp6I GTAC 1 cut(s) 1212
CviAII CATG 6 cut(s) 142, 236, 527, 592, 1199, 1304
CviQI GTAC 1 cut(s) 1212
DdeI CTNAG 3 cut(s) 23, 503, 1316
DpnI GATC 8 cut(s) 152, 250, 256, 261, 266, 290, 1125, 1349
DpnII GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
DrdI GACNNNNNNGTC 2 cut(s) 560, 1329
DseDI GACNNNNNNGTC 2 cut(s) 560, 1329
Eam1104I CTCTTC 2 cut(s) 1031, 1090
EarI CTCTTC 2 cut(s) 1031, 1090
Ecl136II GAGCTC 1 cut(s) 1041
Eco130I CCWWGG 3 cut(s) 130, 216, 1334
Eco24I GRGCYC 1 cut(s) 1043
Eco31I GGTCTC 1 cut(s) 609
Eco47I GGWCC 3 cut(s) 95, 516, 1115
Eco53kI GAGCTC 1 cut(s) 1041
Eco57I CTGAAG 3 cut(s) 178, 440, 792
EcoICRI GAGCTC 1 cut(s) 1041
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 516
EcoRI GAATTC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 145
EcoT14I CCWWGG 3 cut(s) 130, 216, 1334
EcoT38I GRGCYC 1 cut(s) 1043
ErhI CCWWGG 3 cut(s) 130, 216, 1334
Esp3I CGTCTC 1 cut(s) 826
FaeI CATG 6 cut(s) 145, 239, 530, 595, 1202, 1307
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FaqI GGGAC 2 cut(s) 529, 549
FatI CATG 6 cut(s) 141, 235, 526, 591, 1198, 1303
FauI CCCGC 1 cut(s) 673
FauNDI CATATG 1 cut(s) 1179
FblI GTMKAC 4 cut(s) 46, 552, 798, 1321
Fnu4HI GCNGC 6 cut(s) 546, 579, 768, 829, 842, 1223
FokI GGATG 2 cut(s) 233, 1069
FriOI GRGCYC 1 cut(s) 1043
Fsp4HI GCNGC 6 cut(s) 546, 579, 768, 829, 842, 1223
FspBI CTAG 3 cut(s) 14, 41, 81
GlaI GCGC 1 cut(s) 1246
GluI GCNGC 6 cut(s) 546, 579, 768, 829, 842, 1223
GsaI CCCAGC 2 cut(s) 890, 1056
GsuI CTGGAG 1 cut(s) 764
HaeIII GGCC 1 cut(s) 545
HapII CCGG 3 cut(s) 37, 252, 1127
HgaI GACGC 1 cut(s) 178
HhaI GCGC 1 cut(s) 1247
Hin1II CATG 6 cut(s) 145, 239, 530, 595, 1202, 1307
Hin6I GCGC 1 cut(s) 1245
HinP1I GCGC 1 cut(s) 1245
HincII GTYRAC 4 cut(s) 480, 799, 805, 870
HindII GTYRAC 4 cut(s) 480, 799, 805, 870
HindIII AAGCTT 2 cut(s) 1082, 1313
HinfI GANTC 5 cut(s) 86, 351, 454, 919, 981
HpaI GTTAAC 1 cut(s) 480
HpaII CCGG 3 cut(s) 37, 252, 1127
HphI GGTGA 5 cut(s) 119, 145, 913, 1010, 1042
Hpy188I TCNGA 4 cut(s) 259, 264, 375, 453
Hpy188III TCNNGA 6 cut(s) 252, 278, 781, 985, 1127, 1304
Hpy99I CGWCG 5 cut(s) 800, 803, 866, 884, 1030
HpyAV CCTTC 4 cut(s) 319, 679, 718, 889
HpyCH4III ACNGT 6 cut(s) 116, 540, 557, 880, 1216, 1345
HpyCH4IV ACGT 3 cut(s) 476, 489, 801
HpyCH4V TGCA 3 cut(s) 581, 876, 1268
HpyF10VI GCNNNNNNNGC 4 cut(s) 491, 587, 665, 1171
HpyF3I CTNAG 3 cut(s) 23, 503, 1316
HpySE526I ACGT 3 cut(s) 476, 489, 801
Hsp92II CATG 6 cut(s) 145, 239, 530, 595, 1202, 1307
HspAI GCGC 1 cut(s) 1245
KflI GGGWCCC 1 cut(s) 516
Kpn2I TCCGGA 2 cut(s) 251, 1126
KspAI GTTAAC 1 cut(s) 480
Kzo9I GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
LguI GCTCTTC 1 cut(s) 1031
LmnI GCTCC 1 cut(s) 1162
Lsp1109I GCAGC 3 cut(s) 565, 828, 1234
LweI GCATC 1 cut(s) 14
MaeI CTAG 3 cut(s) 14, 41, 81
MaeII ACGT 3 cut(s) 476, 489, 801
MaeIII GTNAC 2 cut(s) 901, 1147
MalI GATC 8 cut(s) 152, 250, 256, 261, 266, 290, 1125, 1349
MboI GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
MflI RGATCY 4 cut(s) 248, 264, 288, 1123
MhlI GDGCHC 1 cut(s) 1043
MluCI AATT 6 cut(s) 299, 319, 458, 856, 1206, 1237
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 353, 523, 657
MroI TCCGGA 2 cut(s) 251, 1126
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 2 cut(s) 525, 881
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 3 cut(s) 37, 252, 1127
MspR9I CCNGG 1 cut(s) 147
Mva1269I GAATGC 3 cut(s) 494, 592, 1247
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 491, 587, 665, 1171
NdeI CATATG 1 cut(s) 1179
NdeII GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
NlaIII CATG 6 cut(s) 145, 239, 530, 595, 1202, 1307
NlaIV GGNNCC 6 cut(s) 32, 290, 517, 518, 1125, 1196
NmuCI GTSAC 1 cut(s) 901
NspI RCATGY 2 cut(s) 145, 530
NspV TTCGAA 3 cut(s) 323, 1087, 1288
OliI CACNNNNGTG 1 cut(s) 881
PaeI GCATGC 1 cut(s) 530
PagI TCATGA 1 cut(s) 1303
PaqCI CACCTGC 1 cut(s) 91
PciSI GCTCTTC 1 cut(s) 1031
PcsI WCGNNNNNNNCGW 2 cut(s) 795, 858
PctI GAATGC 3 cut(s) 494, 592, 1247
PfeI GAWTC 4 cut(s) 351, 454, 919, 981
PkrI GCNGC 6 cut(s) 547, 580, 769, 830, 843, 1224
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PpuMI RGGWCCY 1 cut(s) 516
PshAI GACNNNNGTC 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 1043
Psp1406I AACGTT 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 2 cut(s) 886, 1052
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 6 cut(s) 32, 290, 517, 518, 1125, 1196
PspPI GGNCC 4 cut(s) 95, 516, 543, 1115
PspPPI RGGWCCY 1 cut(s) 516
PsuI RGATCY 4 cut(s) 248, 264, 288, 1123
RsaI GTAC 1 cut(s) 1213
RsaNI GTAC 1 cut(s) 1212
RseI CAYNNNNRTG 2 cut(s) 525, 881
SacI GAGCTC 1 cut(s) 1043
SalI GTCGAC 1 cut(s) 797
SapI GCTCTTC 1 cut(s) 1031
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 6 cut(s) 546, 579, 768, 829, 842, 1223
Sau3AI GATC 8 cut(s) 150, 248, 254, 259, 264, 288, 1123, 1347
Sau96I GGNCC 4 cut(s) 95, 516, 543, 1115
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 1043
SfaNI GCATC 1 cut(s) 14
SfuI TTCGAA 3 cut(s) 323, 1087, 1288
SgrDI CGTCGACG 1 cut(s) 797
SinI GGWCC 3 cut(s) 95, 516, 1115
SmiMI CAYNNNNRTG 2 cut(s) 525, 881
SmlI CTYRAG 1 cut(s) 1189
SmoI CTYRAG 1 cut(s) 1189
SphI GCATGC 1 cut(s) 530
Sse9I AATT 6 cut(s) 299, 319, 458, 856, 1206, 1237
SsiI CCGC 9 cut(s) 98, 156, 546, 619, 657, 666, 768, 808, 829
SspMI CTAG 3 cut(s) 14, 41, 81
SstI GAGCTC 1 cut(s) 1043
StyD4I CCNGG 1 cut(s) 145
StyI CCWWGG 3 cut(s) 130, 216, 1334
TaaI ACNGT 6 cut(s) 116, 540, 557, 880, 1216, 1345
TaiI ACGT 3 cut(s) 479, 492, 804
TaqI TCGA 8 cut(s) 185, 277, 323, 406, 762, 798, 1087, 1288
TasI AATT 6 cut(s) 299, 319, 458, 856, 1206, 1237
TauI GCSGC 3 cut(s) 548, 770, 831
TfiI GAWTC 4 cut(s) 351, 454, 919, 981
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 2 cut(s) 202, 418
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 3 cut(s) 578, 841, 1222
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 3 cut(s) 599, 1196, 1292
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 2 cut(s) 202, 418
VpaK11BI GGWCC 3 cut(s) 95, 516, 1115
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 1 cut(s) 319
XceI RCATGY 2 cut(s) 145, 530
XcmI CCANNNNNNNNNTGG 2 cut(s) 214, 1289
XmiI GTMKAC 4 cut(s) 46, 552, 798, 1321
XspI CTAG 3 cut(s) 14, 41, 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.