Rroxscaffold_5G00352250

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005704
Physical Location & Seq
Reverse (-)
29248723 .. 29255628
6906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352250.1

Sequence Viewer

Length: 1386 bp
ATGAACTCCTCCGCCACCGCCACCGCCACCGCCACCGCCCACTCTCTCGCTCCGTCGCCATTCCACCGCTTCCGCAACGCATGCGCTCCCAAAGCCTCCTTCTCCTGCTCCATGCCTCGCCCTCCCAATCGAAACCGCCGAAACCGCAGACGAAACTCCAACACCGCCACCACCACCACCACCTTCACCACCGGCCCCACCGCCAAGCCCGCCTTCTACACTTCTTCTGACACTGAAAGCTTCCAAGCCACCTTCGACCTCAACAGCCTCTACCACTCCTCCCACTCCTACCTCCGCCACTTCCTCGCCTCCGCCGCCGACGCCGCCGAAGACCTCCAGACGCTCGTCTCCGTCGACGTCGACCGCAGGATCGTCGTCTCCTGCCGCCCCTCCACGCTGCGGTTCGTCGGCAATTTCGTCGTGCTGACCTGCGCCGTCGTGCTGGGGTTTAGGGTTCTGGTTGGGTTGGTGGGGTTAGGGTTTGGGAGCGGGTCGGGTTACGGAAGGGAGAAGGTGGTGACGAGGAGGGATAGGAGTCTTGGGGGGAAGGAGGTTGTGGTAGCTAGGATAGAGAAGCCAAGAGCAGAGGAGGTTAGCGTCAAGAAGAACAGGGGTTTGGGGATTTTGGATACTCCCCTATCGTCGGTGAGGAAGAGCTCGAGCTCAATGGTGGCTGGGATGGAGAGAGTGTTTAAGAATCGGGTTCGCTTCGGGGAGAAGCTTCCCCAATGGTGGCCAACTACAACTTCCCAGCCTATTTTAGGGGTTGATAATGAGGAGCACCAGAGAGAAGCAAACAGATTGGTTAGAGCAATCACCGACAATAGAATGAGCGGGAAGGACATTATGGAGGATGATATAATTCATTTACGTCAAATATGCAGGACATATGGAGTACGGGTCTCCTTTGATACTACAAATACACGGGATTCATTGTACCGTGTGTCTGTTGACTTTGTCTTAAATGTATGCAGCAGGGCACCGAGTTACTCTAAAGGTGTTGAGATCGAGGGTGAAGATGCTCGGCAATTCATAGCTGGGCTAGCTGAGAACATTGGGCTTGAGAATGTTCGTGCTGGAAGAATTGTGTCTGCTGCCGTAGCTGCACGTACACGTTCATGTTTTTTGCAGGCTTGGGCTTTAGTAATGCAAGCTAAACATGCAGAAGCAGTAGTAGAACTGTCAAAGATATGCCTTGTTTGGCGGATTTTCCCTCCCGAGGAATCTGCGCCTGAGATGGAGATGGTGGCTCGAGGCTTGGAGAAACACTTGAAAAAGAGTCAGAGGGAATTTCTTATGAGCATGCTTGTTGGCACTTGCAGTGAAGAGAGCCAGAGAAGAGCAGCAGAAGCGCTGGGTTTGACATCATCTTTCAAAGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

461

Amino Acids

50.91

Weight (kDa)

9.76

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 437
AccB1I GGYRCC 1 cut(s) 979
AccBSI CCGCTC 2 cut(s) 489, 834
AccI GTMKAC 2 cut(s) 354, 360
AclWI GGATC 1 cut(s) 377
AcoI YGGCCR 1 cut(s) 734
AcsI RAATTY 1 cut(s) 1289
AcyI GRCGYC 2 cut(s) 321, 357
AfaI GTAC 3 cut(s) 897, 938, 1111
AfeI AGCGCT 1 cut(s) 1353
AfiI CCNNNNNNNGG 5 cut(s) 399, 643, 732, 761, 1219
AflIII ACRYGT 1 cut(s) 1112
AgsI TTSAA 2 cut(s) 1273, 1375
AjuI GAANNNNNNNTTGG 8 cut(s) 83, 115, 197, 229, 599, 631, 730, 762
AluBI AGCT 9 cut(s) 240, 563, 657, 663, 721, 1037, 1046, 1103, 1154
AluI AGCT 9 cut(s) 240, 563, 657, 663, 721, 1037, 1046, 1103, 1154
Alw21I GWGCWC 3 cut(s) 659, 665, 783
Alw26I GTCTC 3 cut(s) 352, 382, 907
AlwI GGATC 1 cut(s) 377
Ama87I CYCGRG 3 cut(s) 658, 1217, 1251
Aor51HI AGCGCT 1 cut(s) 1353
AoxI GGCC 2 cut(s) 193, 734
ApeKI GCWGC 5 cut(s) 397, 972, 1094, 1103, 1343
ApoI RAATTY 1 cut(s) 1289
AspLEI GCGC 4 cut(s) 86, 434, 1231, 1354
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 178, 529, 658, 808, 1025
AsuNHI GCTAGC 1 cut(s) 1042
AvaI CYCGRG 3 cut(s) 658, 1217, 1251
BaeGI GKGCMC 1 cut(s) 982
BalI TGGCCA 1 cut(s) 736
BanI GGYRCC 1 cut(s) 979
BanII GRGCYC 2 cut(s) 659, 665
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 3 cut(s) 659, 665, 783
BbvI GCAGC 5 cut(s) 384, 984, 1081, 1090, 1355
BccI CCATC 3 cut(s) 673, 1231, 1237
BceAI ACGGC 2 cut(s) 419, 1082
BcgI CGANNNNNNTGC 6 cut(s) 355, 389, 400, 434, 1208, 1242
BciVI GTATCC 1 cut(s) 622
BcoDI GTCTC 3 cut(s) 352, 382, 907
BfaI CTAG 2 cut(s) 564, 1043
BfoI RGCGCY 1 cut(s) 1355
BfuAI ACCTGC 1 cut(s) 437
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 8 cut(s) 315, 324, 385, 398, 973, 1095, 1104, 1344
BlsI GCNGC 8 cut(s) 316, 325, 386, 399, 974, 1096, 1105, 1345
BmeT110I CYCGRG 3 cut(s) 658, 1217, 1251
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 2 cut(s) 196, 981
BmsI GCATC 1 cut(s) 1009
BmtI GCTAGC 1 cut(s) 1046
BoxI GACNNNNGTC 1 cut(s) 344
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 1082
BsaAI YACGTR 1 cut(s) 1109
BsaHI GRCGYC 2 cut(s) 321, 357
BsaI GGTCTC 1 cut(s) 907
BsaJI CCNNGG 1 cut(s) 1218
BsaXI ACNNNNNCTCC 6 cut(s) 263, 293, 674, 704, 885, 915
Bsc4I CCNNNNNNNGG 5 cut(s) 399, 643, 732, 761, 1219
Bse118I RCCGGY 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 1218
BseGI GGATG 2 cut(s) 684, 859
BseLI CCNNNNNNNGG 5 cut(s) 399, 643, 732, 761, 1219
BseMII CTCAG 2 cut(s) 1038, 1224
BseRI GAGGAG 4 cut(s) 268, 538, 602, 791
BseSI GKGCMC 1 cut(s) 982
BseXI GCAGC 5 cut(s) 384, 984, 1081, 1090, 1355
BseYI CCCAGC 5 cut(s) 442, 674, 750, 1037, 1354
BsgI GTGCAG 1 cut(s) 1089
Bsh1285I CGRYCG 1 cut(s) 364
BshFI GGCC 2 cut(s) 195, 736
BshNI GGYRCC 1 cut(s) 979
BsiEI CGRYCG 1 cut(s) 364
BsiHKAI GWGCWC 3 cut(s) 659, 665, 783
BsiHKCI CYCGRG 3 cut(s) 658, 1217, 1251
BsiSI CCGG 1 cut(s) 192
BslI CCNNNNNNNGG 5 cut(s) 399, 643, 732, 761, 1219
BsmAI GTCTC 3 cut(s) 352, 382, 907
BsmBI CGTCTC 2 cut(s) 352, 382
BsnI GGCC 2 cut(s) 195, 736
Bso31I GGTCTC 1 cut(s) 907
BsoBI CYCGRG 3 cut(s) 658, 1217, 1251
Bsp1286I GDGCHC 4 cut(s) 659, 665, 783, 982
Bsp143I GATC 2 cut(s) 369, 1005
BspANI GGCC 2 cut(s) 195, 736
BspCNI CTCAG 2 cut(s) 1039, 1225
BspLI GGNNCC 2 cut(s) 196, 981
BspMI ACCTGC 1 cut(s) 437
BspOI GCTAGC 1 cut(s) 1046
BspPI GGATC 1 cut(s) 377
BspQI GCTCTTC 2 cut(s) 647, 1333
BspT107I GGYRCC 1 cut(s) 979
BspTNI GGTCTC 1 cut(s) 907
BsrBI CCGCTC 2 cut(s) 489, 834
BsrFI RCCGGY 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 1218
BssMI GATC 2 cut(s) 369, 1005
BssNI GRCGYC 2 cut(s) 321, 357
Bst4CI ACNGT 2 cut(s) 941, 1182
Bst6I CTCTTC 3 cut(s) 647, 1320, 1333
BstACI GRCGYC 2 cut(s) 321, 357
BstAPI GCANNNNNTGC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 1109
BstC8I GCNNGC 6 cut(s) 82, 210, 1044, 1131, 1152, 1304
BstDEI CTNAG 2 cut(s) 1047, 1233
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 2 cut(s) 684, 859
BstH2I RGCGCY 1 cut(s) 1355
BstHHI GCGC 4 cut(s) 86, 434, 1231, 1354
BstKTI GATC 2 cut(s) 372, 1008
BstMAI GTCTC 3 cut(s) 352, 382, 907
BstMBI GATC 2 cut(s) 369, 1005
BstMCI CGRYCG 1 cut(s) 364
BstNSI RCATGY 3 cut(s) 84, 1163, 1306
BstPAI GACNNNNGTC 1 cut(s) 344
BstSLI GKGCMC 1 cut(s) 982
BstV1I GCAGC 5 cut(s) 384, 984, 1081, 1090, 1355
BstV2I GAAGAC 1 cut(s) 336
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 195, 736
BtsCI GGATG 2 cut(s) 684, 859
BtsI GCAGTG 1 cut(s) 1327
BtsIMutI CAGTG 2 cut(s) 231, 1327
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 6 cut(s) 82, 210, 1044, 1131, 1152, 1304
CfoI GCGC 4 cut(s) 86, 434, 1231, 1354
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 194
CseI GACGC 3 cut(s) 329, 349, 586
Csp6I GTAC 3 cut(s) 896, 937, 1110
CviAII CATG 5 cut(s) 81, 112, 1119, 1160, 1303
CviQI GTAC 3 cut(s) 896, 937, 1110
DdeI CTNAG 2 cut(s) 1047, 1233
DpnI GATC 2 cut(s) 371, 1007
DpnII GATC 2 cut(s) 369, 1005
EaeI YGGCCR 1 cut(s) 734
Eam1104I CTCTTC 3 cut(s) 647, 1320, 1333
EarI CTCTTC 3 cut(s) 647, 1320, 1333
EciI GGCGGA 3 cut(s) 284, 301, 1219
Ecl136II GAGCTC 2 cut(s) 657, 663
Eco24I GRGCYC 2 cut(s) 659, 665
Eco31I GGTCTC 1 cut(s) 907
Eco47III AGCGCT 1 cut(s) 1353
Eco53kI GAGCTC 2 cut(s) 657, 663
Eco88I CYCGRG 3 cut(s) 658, 1217, 1251
EcoICRI GAGCTC 2 cut(s) 657, 663
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoT38I GRGCYC 2 cut(s) 659, 665
Esp3I CGTCTC 2 cut(s) 352, 382
FaeI CATG 5 cut(s) 84, 115, 1122, 1163, 1306
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 5 cut(s) 80, 111, 1118, 1159, 1302
FauI CCCGC 3 cut(s) 217, 482, 827
FauNDI CATATG 1 cut(s) 889
FblI GTMKAC 2 cut(s) 354, 360
Fnu4HI GCNGC 8 cut(s) 315, 324, 385, 398, 973, 1095, 1104, 1344
FokI GGATG 2 cut(s) 691, 866
FriOI GRGCYC 2 cut(s) 659, 665
Fsp4HI GCNGC 8 cut(s) 315, 324, 385, 398, 973, 1095, 1104, 1344
FspBI CTAG 2 cut(s) 564, 1043
GlaI GCGC 4 cut(s) 85, 433, 1230, 1353
GluI GCNGC 8 cut(s) 315, 324, 385, 398, 973, 1095, 1104, 1344
GsaI CCCAGC 5 cut(s) 446, 678, 754, 1041, 1358
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 1355
HaeIII GGCC 2 cut(s) 195, 736
HapII CCGG 1 cut(s) 192
HgaI GACGC 3 cut(s) 329, 349, 586
HhaI GCGC 4 cut(s) 86, 434, 1231, 1354
Hin1I GRCGYC 2 cut(s) 321, 357
Hin1II CATG 5 cut(s) 84, 115, 1122, 1163, 1306
Hin6I GCGC 4 cut(s) 84, 432, 1229, 1352
HinP1I GCGC 4 cut(s) 84, 432, 1229, 1352
HincII GTYRAC 3 cut(s) 355, 361, 952
HindII GTYRAC 3 cut(s) 355, 361, 952
HindIII AAGCTT 2 cut(s) 238, 719
HinfI GANTC 5 cut(s) 535, 697, 929, 1223, 1279
HpaII CCGG 1 cut(s) 192
HphI GGTGA 5 cut(s) 178, 529, 658, 808, 1025
Hpy166II GTNNAC 4 cut(s) 355, 361, 952, 1112
Hpy188I TCNGA 2 cut(s) 229, 1284
Hpy188III TCNNGA 3 cut(s) 337, 601, 1217
Hpy8I GTNNAC 4 cut(s) 355, 361, 952, 1112
HpyAV CCTTC 8 cut(s) 109, 193, 223, 262, 498, 505, 541, 832
HpyCH4III ACNGT 2 cut(s) 941, 1182
HpyCH4IV ACGT 4 cut(s) 357, 871, 1108, 1114
HpyCH4V TGCA 7 cut(s) 882, 972, 1106, 1129, 1150, 1163, 1320
HpyF3I CTNAG 2 cut(s) 1047, 1233
HpySE526I ACGT 4 cut(s) 357, 871, 1108, 1114
Hsp92I GRCGYC 2 cut(s) 321, 357
Hsp92II CATG 5 cut(s) 84, 115, 1122, 1163, 1306
HspAI GCGC 4 cut(s) 84, 432, 1229, 1352
Kzo9I GATC 2 cut(s) 369, 1005
LguI GCTCTTC 2 cut(s) 647, 1333
LmnI GCTCC 5 cut(s) 55, 91, 113, 486, 778
Lsp1109I GCAGC 5 cut(s) 384, 984, 1081, 1090, 1355
LweI GCATC 1 cut(s) 1009
MaeI CTAG 2 cut(s) 564, 1043
MaeII ACGT 4 cut(s) 357, 871, 1108, 1114
MaeIII GTNAC 3 cut(s) 497, 517, 986
MalI GATC 2 cut(s) 371, 1007
MbiI CCGCTC 2 cut(s) 489, 834
MboI GATC 2 cut(s) 369, 1005
MboII GAAGA 8 cut(s) 216, 341, 616, 664, 1028, 1092, 1337, 1350
MhlI GDGCHC 4 cut(s) 659, 665, 783, 982
MlsI TGGCCA 1 cut(s) 736
MluCI AATT 5 cut(s) 412, 861, 1028, 1083, 1289
MluNI TGGCCA 1 cut(s) 736
MlyI GAGTC 2 cut(s) 544, 1288
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 736
MscI TGGCCA 1 cut(s) 736
MseI TTAA 2 cut(s) 693, 962
MslI CAYNNNNRTG 1 cut(s) 1117
Msp20I TGGCCA 1 cut(s) 736
MspI CCGG 1 cut(s) 192
NdeI CATATG 1 cut(s) 889
NdeII GATC 2 cut(s) 369, 1005
NheI GCTAGC 1 cut(s) 1042
NlaIII CATG 5 cut(s) 84, 115, 1122, 1163, 1306
NlaIV GGNNCC 2 cut(s) 196, 981
NmeAIII GCCGAG 1 cut(s) 1003
NmuCI GTSAC 1 cut(s) 517
NspI RCATGY 3 cut(s) 84, 1163, 1306
PaeI GCATGC 2 cut(s) 84, 1306
PaeR7I CTCGAG 2 cut(s) 658, 1251
PciSI GCTCTTC 2 cut(s) 647, 1333
PcsI WCGNNNNNNNCGW 3 cut(s) 136, 351, 414
PfeI GAWTC 3 cut(s) 697, 929, 1223
PflFI GACNNNGTC 1 cut(s) 956
PkrI GCNGC 8 cut(s) 316, 325, 386, 399, 974, 1096, 1105, 1345
PleI GAGTC 2 cut(s) 543, 1287
PpsI GAGTC 2 cut(s) 543, 1287
Ppu21I YACGTR 1 cut(s) 1109
PshAI GACNNNNGTC 1 cut(s) 344
Psp124BI GAGCTC 2 cut(s) 659, 665
PspFI CCCAGC 5 cut(s) 442, 674, 750, 1037, 1354
PspN4I GGNNCC 2 cut(s) 196, 981
PspPI GGNCC 1 cut(s) 194
PspXI VCTCGAGB 2 cut(s) 658, 1251
PsyI GACNNNGTC 1 cut(s) 956
RsaI GTAC 3 cut(s) 897, 938, 1111
RsaNI GTAC 3 cut(s) 896, 937, 1110
RseI CAYNNNNRTG 1 cut(s) 1117
SacI GAGCTC 2 cut(s) 659, 665
SalI GTCGAC 2 cut(s) 353, 359
SapI GCTCTTC 2 cut(s) 647, 1333
SaqAI TTAA 2 cut(s) 693, 962
SatI GCNGC 8 cut(s) 315, 324, 385, 398, 973, 1095, 1104, 1344
Sau3AI GATC 2 cut(s) 369, 1005
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 2 cut(s) 544, 1288
SduI GDGCHC 4 cut(s) 659, 665, 783, 982
SfaNI GCATC 1 cut(s) 1009
Sfr274I CTCGAG 2 cut(s) 658, 1251
SgrDI CGTCGACG 1 cut(s) 353
SlaI CTCGAG 2 cut(s) 658, 1251
SmiMI CAYNNNNRTG 1 cut(s) 1117
SmlI CTYRAG 3 cut(s) 658, 1061, 1251
SmoI CTYRAG 3 cut(s) 658, 1061, 1251
SphI GCATGC 2 cut(s) 84, 1306
Sse9I AATT 5 cut(s) 412, 861, 1028, 1083, 1289
SspMI CTAG 2 cut(s) 564, 1043
SstI GAGCTC 2 cut(s) 659, 665
TaaI ACNGT 2 cut(s) 941, 1182
TaiI ACGT 4 cut(s) 360, 874, 1111, 1117
TaqI TCGA 7 cut(s) 130, 255, 354, 360, 659, 1008, 1252
TasI AATT 5 cut(s) 412, 861, 1028, 1083, 1289
TauI GCSGC 3 cut(s) 317, 326, 387
TfiI GAWTC 3 cut(s) 697, 929, 1223
Tru1I TTAA 2 cut(s) 693, 962
Tru9I TTAA 2 cut(s) 693, 962
TscAI CASTG 2 cut(s) 238, 1327
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 5 cut(s) 397, 972, 1094, 1103, 1343
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 5 cut(s) 17, 854, 921, 1021, 1107
TspGWI ACGGA 3 cut(s) 42, 340, 516
TspRI CASTG 2 cut(s) 238, 1327
Tth111I GACNNNGTC 1 cut(s) 956
XagI CCTNNNNNAGG 1 cut(s) 759
XapI RAATTY 1 cut(s) 1289
XceI RCATGY 3 cut(s) 84, 1163, 1306
XhoI CTCGAG 2 cut(s) 658, 1251
XmiI GTMKAC 2 cut(s) 354, 360
XspI CTAG 2 cut(s) 564, 1043
ZraI GACGTC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.