pycom04g03090

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005704
Physical Location & Seq
Forward (+)
2866103 .. 2870955
4853 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03090.6

Sequence Viewer

Length: 1398 bp
ATGAACTTCTCCACCCACGCTCACGCTCTCACCACTCACTCTCCACTCCACCGCTTCACCACCGCCGCCGTCCCCGCCCCATGTCAGTCCTCCTTCTCCTGCTCCATGCCCCGTCCCCCCAATCGCAACCGCAGAACTCGCCGCAATCGAAACACCACCCGCCCCACCACCACCACCACTACCACCTCCACCACCATTAAACCCTATTTCTACCCTCCTTCACCATCCAACCCTGATACTCTCCAAGCCACCTTCGACCTCGATTACATCTACCACACCTCCCACTCCACCCTCAACCAATTCATCTCCGACTCCTCCGACGCCCTCCAAGACCTCCGCACTCTCGTCTCTGTCGACGCCGGTAATCGCGTCATCGTCTCATGTCGCCCTTCCACGCTGCGTTTCGTCGGCAATTTGGTGATAATCACCTTCTCCGTCGTTTTGGGGTTTAGGGTTTTGGTTGCCTTGTTCAGGTTAGGGTTTAGGAGTCGGTCTGGGTATGGCAAGGAGGCGACGGTGGTGAGGCGGGATAGGAGTCTCGGGGGAAAAGAGGTTGTGGTAGGGATAGTGGAGAGGGAGAGAGTTGATGCGAGGAAAAGAAGGAGTTTTGGGATTTTGGATAATCCGCTGTCAATGCCGAAGAGTAGGGTGGTGGATGGTTTGGGGAGGATGTTAAAGAATCAGGTTCGGTGGGCGAAGAAGCTGCCGAGCTGGTGGCCGAGCTCAGCACCGCAGCAGAGTCCGGTGGTGGATAAGGAGTACTACCAGGGAGAAGCAGACAGATTGGTTAGAGTGATCACGGACAATAGAATGAGCGGAAAGGACATAGGGGAGGATGACATTATTCATTTACGTCAAATATGCAGGACATCTGGAGTACGGGTCTCCTTTGATACAACAAATACACGAGATTCGTTCTACCGGGTGTCTGTTGAATATGTCTTAAATGTTTGCAGCAGGGCCCCAAGTCATTCTACTTATGTTCGGATTGACGGAGAAGATGTTTGTCATTTCATTGCTGGGCTTGCCGGGAATATTGGCCTTGACAACATTCGTGCTGCAAGAATTATGTCTGCAGCAGTGGCTGCACGTACACGCTCACGTTTTCTGCAGGCATGGGCATTGGTAATGCAAAGTAAACATGCAGAAGCACTTGTCGAACTGTCAAAGATATGCCTGCTTTTGCGGATATTCCCTCCCGAGGAGTCCTCTCCTGAGATGGAGATGGTAGCTCGAGGCTTGGAAAAACATTTGAAATTGGATCAGAGGGAATTTCTAATGAGCATGCTCACTGGCGTTTGCAGTGAGGAAAGCCAAAGAAGAGCAGCAGAAGCTTTGGGTTTGAACCCCGACGGACAATTTTTGGGGCTGGACGGGGAAATCATGGATGTCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

466

Amino Acids

52.08

Weight (kDa)

9.64

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 816
AccI GTMKAC 1 cut(s) 354
AccII CGCG 1 cut(s) 369
AclWI GGATC 1 cut(s) 1269
AcoI YGGCCR 1 cut(s) 716
AcsI RAATTY 1 cut(s) 1271
AcyI GRCGYC 2 cut(s) 321, 357
AfaI GTAC 3 cut(s) 761, 879, 1093
AfiI CCNNNNNNNGG 2 cut(s) 471, 1201
AgsI TTSAA 3 cut(s) 935, 1255, 1345
AjnI CCWGG 1 cut(s) 765
AluBI AGCT 5 cut(s) 703, 711, 723, 1232, 1334
AluI AGCT 5 cut(s) 703, 711, 723, 1232, 1334
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 4 cut(s) 352, 382, 542, 889
AlwI GGATC 1 cut(s) 1269
AlwNI CAGNNNCTG 1 cut(s) 1085
Ama87I CYCGRG 3 cut(s) 539, 1199, 1233
AoxI GGCC 3 cut(s) 716, 960, 1039
ApaI GGGCCC 1 cut(s) 964
ApeKI GCWGC 8 cut(s) 397, 703, 733, 954, 1058, 1076, 1085, 1325
ApoI RAATTY 1 cut(s) 1271
AspS9I GGNCC 2 cut(s) 960, 961
AsuC2I CCSGG 2 cut(s) 923, 1030
AsuHPI GGTGA 6 cut(s) 22, 49, 213, 418, 430, 532
AvaI CYCGRG 3 cut(s) 539, 1199, 1233
BaeGI GKGCMC 1 cut(s) 964
BanII GRGCYC 2 cut(s) 725, 964
BauI CACGAG 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 8 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1337
BccI CCATC 4 cut(s) 232, 650, 1213, 1219
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 1 cut(s) 767
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 2 cut(s) 923, 1030
BcoDI GTCTC 4 cut(s) 352, 382, 542, 889
BfmI CTRYAG 2 cut(s) 1074, 1109
BlpI GCTNAGC 1 cut(s) 724
BmcAI AGTACT 1 cut(s) 761
Bme1390I CCNGG 3 cut(s) 767, 923, 1030
BmeT110I CYCGRG 3 cut(s) 539, 1199, 1233
BmgT120I GGNCC 2 cut(s) 960, 961
BmiI GGNNCC 2 cut(s) 962, 963
BmrFI CCNGG 3 cut(s) 767, 923, 1030
BmsI GCATC 1 cut(s) 577
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
BpmI CTGGAG 1 cut(s) 894
Bpu1102I GCTNAGC 1 cut(s) 724
BpuMI CCSGG 2 cut(s) 923, 1030
BsaAI YACGTR 1 cut(s) 1091
BsaHI GRCGYC 2 cut(s) 321, 357
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 2 cut(s) 766, 1200
BsaWI WCCGGW 1 cut(s) 742
BsaXI ACNNNNNCTCC 8 cut(s) 25, 55, 263, 293, 867, 897, 987, 1017
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 1201
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 1297
Bse3DI GCAATG 1 cut(s) 1014
BseBI CCWGG 1 cut(s) 767
BseDI CCNNGG 2 cut(s) 766, 1200
BseGI GGATG 5 cut(s) 224, 661, 675, 841, 1393
BseLI CCNNNNNNNGG 2 cut(s) 471, 1201
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 2 cut(s) 738, 1206
BseNI ACTGG 1 cut(s) 1297
BseRI GAGGAG 2 cut(s) 304, 1217
BseSI GKGCMC 1 cut(s) 964
BseXI GCAGC 8 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1337
BseYI CCCAGC 1 cut(s) 1019
BsgI GTGCAG 1 cut(s) 1071
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 3 cut(s) 718, 962, 1041
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 3 cut(s) 539, 1199, 1233
BsiSI CCGG 4 cut(s) 360, 743, 922, 1029
BslFI GGGAC 2 cut(s) 56, 99
BslI CCNNNNNNNGG 2 cut(s) 471, 1201
BsmAI GTCTC 4 cut(s) 352, 382, 542, 889
BsmBI CGTCTC 2 cut(s) 352, 382
BsmFI GGGAC 2 cut(s) 56, 99
BsnI GGCC 3 cut(s) 718, 962, 1041
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 3 cut(s) 539, 1199, 1233
Bsp120I GGGCCC 1 cut(s) 960
Bsp1286I GDGCHC 2 cut(s) 725, 964
Bsp143I GATC 2 cut(s) 795, 1261
Bsp1720I GCTNAGC 1 cut(s) 724
BspANI GGCC 3 cut(s) 718, 962, 1041
BspCNI CTCAG 2 cut(s) 737, 1207
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 962, 963
BspMAI CTGCAG 2 cut(s) 1078, 1113
BspPI GGATC 1 cut(s) 1269
BspQI GCTCTTC 1 cut(s) 1315
BspTNI GGTCTC 1 cut(s) 889
BsrBI CCGCTC 1 cut(s) 816
BsrDI GCAATG 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 1 cut(s) 1297
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 766, 1200
BssMI GATC 2 cut(s) 795, 1261
BssNI GRCGYC 2 cut(s) 321, 357
BssSI CACGAG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 2 cut(s) 517, 1164
Bst6I CTCTTC 2 cut(s) 635, 1315
BstACI GRCGYC 2 cut(s) 321, 357
BstAPI GCANNNNNTGC 1 cut(s) 1085
BstBAI YACGTR 1 cut(s) 1091
BstC8I GCNNGC 4 cut(s) 1026, 1113, 1178, 1286
BstDEI CTNAG 2 cut(s) 724, 1215
BstENI CCTNNNNNAGG 1 cut(s) 469
BstF5I GGATG 5 cut(s) 224, 661, 675, 841, 1393
BstFNI CGCG 1 cut(s) 369
BstKTI GATC 2 cut(s) 798, 1264
BstMAI GTCTC 4 cut(s) 352, 382, 542, 889
BstMBI GATC 2 cut(s) 795, 1261
BstMWI GCNNNNNNNGC 7 cut(s) 74, 138, 634, 1025, 1082, 1085, 1331
BstNI CCWGG 1 cut(s) 767
BstNSI RCATGY 2 cut(s) 1145, 1288
BstSCI CCNGG 3 cut(s) 765, 921, 1028
BstSFI CTRYAG 2 cut(s) 1074, 1109
BstSLI GKGCMC 1 cut(s) 964
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 8 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1337
BsuRI GGCC 3 cut(s) 718, 962, 1041
BtsCI GGATG 5 cut(s) 224, 661, 675, 841, 1393
BtsI GCAGTG 2 cut(s) 1086, 1309
BtsIMutI CAGTG 3 cut(s) 1086, 1290, 1309
Cac8I GCNNGC 4 cut(s) 1026, 1113, 1178, 1286
CaiI CAGNNNCTG 1 cut(s) 1085
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 960, 961
CseI GACGC 3 cut(s) 329, 358, 365
Csp6I GTAC 3 cut(s) 760, 878, 1092
CviAII CATG 7 cut(s) 81, 106, 381, 1116, 1142, 1285, 1384
CviQI GTAC 3 cut(s) 760, 878, 1092
DdeI CTNAG 2 cut(s) 724, 1215
DpnI GATC 2 cut(s) 797, 1263
DpnII GATC 2 cut(s) 795, 1261
EaeI YGGCCR 1 cut(s) 716
Eam1104I CTCTTC 2 cut(s) 635, 1315
EarI CTCTTC 2 cut(s) 635, 1315
Ecl136II GAGCTC 1 cut(s) 723
Eco24I GRGCYC 2 cut(s) 725, 964
Eco31I GGTCTC 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 723
Eco88I CYCGRG 3 cut(s) 539, 1199, 1233
EcoICRI GAGCTC 1 cut(s) 723
EcoNI CCTNNNNNAGG 1 cut(s) 469
EcoO109I RGGNCCY 2 cut(s) 960, 961
EcoRII CCWGG 1 cut(s) 765
EcoT38I GRGCYC 2 cut(s) 725, 964
Esp3I CGTCTC 2 cut(s) 352, 382
FaeI CATG 7 cut(s) 84, 109, 384, 1119, 1145, 1288, 1387
FaqI GGGAC 2 cut(s) 56, 99
FatI CATG 7 cut(s) 80, 105, 380, 1115, 1141, 1284, 1383
FauI CCCGC 3 cut(s) 82, 167, 519
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 1 cut(s) 354
FokI GGATG 4 cut(s) 211, 668, 682, 848
FriOI GRGCYC 2 cut(s) 725, 964
GsaI CCCAGC 1 cut(s) 1023
GsuI CTGGAG 1 cut(s) 894
HaeIII GGCC 3 cut(s) 718, 962, 1041
HapII CCGG 4 cut(s) 360, 743, 922, 1029
HgaI GACGC 3 cut(s) 329, 358, 365
Hin1I GRCGYC 2 cut(s) 321, 357
Hin1II CATG 7 cut(s) 84, 109, 384, 1119, 1145, 1288, 1387
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 1332
HinfI GANTC 7 cut(s) 311, 487, 535, 679, 739, 911, 1205
HpaII CCGG 4 cut(s) 360, 743, 922, 1029
HphI GGTGA 6 cut(s) 22, 49, 213, 418, 430, 532
Hpy166II GTNNAC 3 cut(s) 355, 1094, 1139
Hpy188I TCNGA 4 cut(s) 310, 319, 987, 1266
Hpy188III TCNNGA 3 cut(s) 873, 1199, 1214
Hpy8I GTNNAC 3 cut(s) 355, 1094, 1139
Hpy99I CGWCG 6 cut(s) 323, 359, 410, 440, 517, 1355
HpyAV CCTTC 6 cut(s) 103, 228, 262, 399, 439, 594
HpyCH4III ACNGT 2 cut(s) 517, 1164
HpyCH4IV ACGT 3 cut(s) 853, 1090, 1102
HpyCH4V TGCA 9 cut(s) 864, 954, 1061, 1076, 1088, 1111, 1132, 1145, 1302
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 138, 634, 1025, 1082, 1085, 1331
HpyF3I CTNAG 2 cut(s) 724, 1215
HpySE526I ACGT 3 cut(s) 853, 1090, 1102
Hsp92I GRCGYC 2 cut(s) 321, 357
Hsp92II CATG 7 cut(s) 84, 109, 384, 1119, 1145, 1288, 1387
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 2 cut(s) 795, 1261
LguI GCTCTTC 1 cut(s) 1315
LmnI GCTCC 1 cut(s) 107
Lsp1109I GCAGC 8 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1337
LweI GCATC 1 cut(s) 577
MaeII ACGT 3 cut(s) 853, 1090, 1102
MalI GATC 2 cut(s) 797, 1263
MbiI CCGCTC 1 cut(s) 816
MboI GATC 2 cut(s) 795, 1261
MboII GAAGA 4 cut(s) 652, 709, 1010, 1332
MhlI GDGCHC 2 cut(s) 725, 964
MluCI AATT 6 cut(s) 299, 412, 1065, 1256, 1271, 1358
MlyI GAGTC 5 cut(s) 305, 496, 544, 748, 1214
MmeI TCCRAC 3 cut(s) 252, 333, 342
MseI TTAA 3 cut(s) 198, 674, 944
MspA1I CMGCKG 1 cut(s) 628
MspI CCGG 4 cut(s) 360, 743, 922, 1029
MspR9I CCNGG 3 cut(s) 767, 923, 1030
MvaI CCWGG 1 cut(s) 767
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 7 cut(s) 74, 138, 634, 1025, 1082, 1085, 1331
NciI CCSGG 2 cut(s) 923, 1030
NdeII GATC 2 cut(s) 795, 1261
NlaIII CATG 7 cut(s) 84, 109, 384, 1119, 1145, 1288, 1387
NlaIV GGNNCC 2 cut(s) 962, 963
NmeAIII GCCGAG 2 cut(s) 732, 744
NspI RCATGY 2 cut(s) 1145, 1288
PaeI GCATGC 1 cut(s) 1288
PaeR7I CTCGAG 1 cut(s) 1233
PciSI GCTCTTC 1 cut(s) 1315
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 351
PfeI GAWTC 2 cut(s) 679, 911
PleI GAGTC 5 cut(s) 305, 495, 543, 747, 1213
PpsI GAGTC 5 cut(s) 305, 495, 543, 747, 1213
Ppu21I YACGTR 1 cut(s) 1091
Psp124BI GAGCTC 1 cut(s) 725
Psp6I CCWGG 1 cut(s) 765
PspFI CCCAGC 1 cut(s) 1019
PspGI CCWGG 1 cut(s) 765
PspN4I GGNNCC 2 cut(s) 962, 963
PspOMI GGGCCC 1 cut(s) 960
PspPI GGNCC 2 cut(s) 960, 961
PspXI VCTCGAGB 1 cut(s) 1233
PstI CTGCAG 2 cut(s) 1078, 1113
PstNI CAGNNNCTG 1 cut(s) 1085
RsaI GTAC 3 cut(s) 761, 879, 1093
RsaNI GTAC 3 cut(s) 760, 878, 1092
SacI GAGCTC 1 cut(s) 725
SalI GTCGAC 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 1315
SaqAI TTAA 3 cut(s) 198, 674, 944
Sau3AI GATC 2 cut(s) 795, 1261
Sau96I GGNCC 2 cut(s) 960, 961
ScaI AGTACT 1 cut(s) 761
SchI GAGTC 5 cut(s) 305, 496, 544, 748, 1214
ScrFI CCNGG 3 cut(s) 767, 923, 1030
SduI GDGCHC 2 cut(s) 725, 964
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 2 cut(s) 1074, 1109
Sfr274I CTCGAG 1 cut(s) 1233
SlaI CTCGAG 1 cut(s) 1233
SmlI CTYRAG 1 cut(s) 1233
SmoI CTYRAG 1 cut(s) 1233
SphI GCATGC 1 cut(s) 1288
Sse9I AATT 6 cut(s) 299, 412, 1065, 1256, 1271, 1358
SspI AATATT 1 cut(s) 1036
SstI GAGCTC 1 cut(s) 725
StyD4I CCNGG 3 cut(s) 765, 921, 1028
TaaI ACNGT 2 cut(s) 517, 1164
TaiI ACGT 3 cut(s) 856, 1093, 1105
TaqI TCGA 6 cut(s) 148, 255, 261, 354, 1158, 1234
TaqII GACCGA 1 cut(s) 480
TasI AATT 6 cut(s) 299, 412, 1065, 1256, 1271, 1358
TatI WGTACW 1 cut(s) 759
TauI GCSGC 2 cut(s) 68, 144
TfiI GAWTC 2 cut(s) 679, 911
Tru1I TTAA 3 cut(s) 198, 674, 944
Tru9I TTAA 3 cut(s) 198, 674, 944
TscAI CASTG 3 cut(s) 1086, 1297, 1309
TseI GCWGC 8 cut(s) 397, 703, 733, 954, 1058, 1076, 1085, 1325
TspDTI ATGAA 4 cut(s) 17, 292, 836, 1003
TspGWI ACGGA 4 cut(s) 424, 815, 1008, 1368
TspRI CASTG 3 cut(s) 1086, 1297, 1309
XagI CCTNNNNNAGG 1 cut(s) 469
XapI RAATTY 1 cut(s) 1271
XceI RCATGY 2 cut(s) 1145, 1288
XhoI CTCGAG 1 cut(s) 1233
XmiI GTMKAC 1 cut(s) 354
ZrmI AGTACT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.