Rmu_sc0001900.1_g000058

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001900.1
OG0005704
Physical Location & Seq
Reverse (-)
285252 .. 288283
3032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001900.1_g000058.1.cds

Sequence Viewer

Length: 753 bp
atggacgtatggctccataaaaccgccctacaaaggaaggaaaaccatcatattgatgcgcggacatgtggcaacgttggccagcgtgggagaggcaaagacggtgcagccgatagtggcggtgccaccaccaacatccctaggggacatcgaaatgcacaaagtacatctcaattaatgggtgagcccgccttctacacttcttccgaccctgaaaacttccaaaccaccttcgacctcaatagcctctacaactcctcccactcctacctcagccacttcctcgcctccgccaatgacctccagaacctcgtcttcttcgacgttgactgcaggatcgtcgtctccttccgcccctccacgctgcgattaatcggcaatttcatcgtgttgacctgcgcagtcatgatgggggagaagttagtgacgaggagggataggagtcttggcgggaaagaggttgtggtagcgaggatagagaagccaatagcagaggacgttagaatcaagaagaacaagggtttggggattttggataatcacctatcgtcggtgaggaagagctcaatggtggctgggatggagagagtgtttaagaattggattcgcttgaggaagaagaagaggagaggaggagagaggttgacacgctggtcacatgttgcgctatcatccactcattgtgctacttatgataaactcctggcccaaatcatcttgctacgggctcactactcagatcatctcatttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

28.13

Weight (kDa)

10.01

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 652
Acc16I TGCGCA 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 122
AccII CGCG 1 cut(s) 61
AciI CCGC 7 cut(s) 24, 61, 120, 189, 291, 352, 450
AclI AACGTT 1 cut(s) 75
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 79
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 2 cut(s) 33, 550
AflIII ACRYGT 2 cut(s) 65, 658
AjnI CCWGG 1 cut(s) 702
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 1 cut(s) 564
AluI AGCT 1 cut(s) 564
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 1 cut(s) 349
AlwI GGATC 1 cut(s) 344
AoxI GGCC 2 cut(s) 79, 705
ApeKI GCWGC 2 cut(s) 107, 364
AseI ATTAAT 2 cut(s) 176, 371
AspA2I CCTAGG 1 cut(s) 140
AspLEI GCGC 3 cut(s) 61, 401, 667
AspS9I GGNCC 1 cut(s) 706
AsuHPI GGTGA 3 cut(s) 194, 533, 565
AvrII CCTAGG 1 cut(s) 140
BalI TGGCCA 1 cut(s) 81
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 3 cut(s) 189, 566, 730
BbsI GAAGAC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 566
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 119, 351
BccI CCATC 3 cut(s) 54, 403, 574
BcgI CGANNNNNNTGC 4 cut(s) 322, 356, 367, 401
BciT130I CCWGG 1 cut(s) 704
BcoDI GTCTC 1 cut(s) 349
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 331
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 2 cut(s) 108, 365
BlnI CCTAGG 1 cut(s) 140
BlsI GCNGC 2 cut(s) 109, 366
Bme1390I CCNGG 1 cut(s) 704
BmgT120I GGNCC 1 cut(s) 706
BmiI GGNNCC 2 cut(s) 14, 124
BmrFI CCNGG 1 cut(s) 704
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 287
Bpu10I CCTNAGC 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 631
BsaJI CCNNGG 1 cut(s) 140
BsaXI ACNNNNNCTCC 4 cut(s) 242, 272, 575, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 550
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 3 cut(s) 135, 585, 671
BseLI CCNNNNNNNGG 2 cut(s) 33, 550
BseMII CTCAG 2 cut(s) 286, 750
BseRI GAGGAG 5 cut(s) 247, 445, 640, 645, 648
BseXI GCAGC 2 cut(s) 119, 351
BseYI CCCAGC 1 cut(s) 575
BsgI GTGCAG 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 2 cut(s) 81, 707
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 566
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 33, 550
BsmAI GTCTC 1 cut(s) 349
BsmBI CGTCTC 1 cut(s) 349
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 2 cut(s) 81, 707
Bsp1286I GDGCHC 3 cut(s) 189, 566, 730
Bsp143I GATC 2 cut(s) 336, 739
BspACI CCGC 7 cut(s) 24, 61, 120, 189, 291, 352, 450
BspANI GGCC 2 cut(s) 81, 707
BspCNI CTCAG 2 cut(s) 285, 749
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 405
BspLI GGNNCC 2 cut(s) 14, 124
BspMAI CTGCAG 1 cut(s) 335
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 2 cut(s) 336, 739
BssT1I CCWWGG 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 2 cut(s) 554, 617
BstC8I GCNNGC 2 cut(s) 83, 189
BstDEI CTNAG 2 cut(s) 272, 736
BstF5I GGATG 3 cut(s) 135, 585, 671
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 3 cut(s) 61, 401, 667
BstKTI GATC 2 cut(s) 339, 742
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 2 cut(s) 336, 739
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 2 cut(s) 69, 662
BstSCI CCNGG 1 cut(s) 702
BstSFI CTRYAG 1 cut(s) 331
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 119, 351
BstV2I GAAGAC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 81, 707
BtsCI GGATG 3 cut(s) 135, 585, 671
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 2 cut(s) 83, 189
CciI TCATGA 1 cut(s) 405
CfoI GCGC 3 cut(s) 61, 401, 667
Cfr13I GGNCC 1 cut(s) 706
Csp6I GTAC 1 cut(s) 165
CviAII CATG 3 cut(s) 66, 406, 659
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 2 cut(s) 272, 736
DpnI GATC 2 cut(s) 338, 741
DpnII GATC 2 cut(s) 336, 739
DrdI GACNNNNNNGTC 1 cut(s) 652
DseDI GACNNNNNNGTC 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 2 cut(s) 554, 617
EarI CTCTTC 2 cut(s) 554, 617
EciI GGCGGA 2 cut(s) 280, 341
Ecl136II GAGCTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 140
Eco24I GRGCYC 3 cut(s) 189, 566, 730
Eco53kI GAGCTC 1 cut(s) 564
EcoICRI GAGCTC 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 702
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 3 cut(s) 189, 566, 730
ErhI CCWWGG 1 cut(s) 140
Esp3I CGTCTC 1 cut(s) 349
FaeI CATG 3 cut(s) 69, 409, 662
FaiI YATR 7 cut(s) 10, 18, 51, 67, 407, 660, 693
FaqI GGGAC 1 cut(s) 159
FatI CATG 3 cut(s) 65, 405, 658
FauI CCCGC 2 cut(s) 196, 443
Fnu4HI GCNGC 2 cut(s) 108, 365
FokI GGATG 3 cut(s) 122, 592, 658
FriOI GRGCYC 3 cut(s) 189, 566, 730
Fsp4HI GCNGC 2 cut(s) 108, 365
FspBI CTAG 1 cut(s) 141
FspI TGCGCA 1 cut(s) 400
GlaI GCGC 3 cut(s) 60, 400, 666
GluI GCNGC 2 cut(s) 108, 365
GsaI CCCAGC 1 cut(s) 579
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 2 cut(s) 81, 707
HhaI GCGC 3 cut(s) 61, 401, 667
Hin1II CATG 3 cut(s) 69, 409, 662
Hin6I GCGC 3 cut(s) 59, 399, 665
HinP1I GCGC 3 cut(s) 59, 399, 665
HincII GTYRAC 3 cut(s) 328, 393, 645
HindII GTYRAC 3 cut(s) 328, 393, 645
HinfI GANTC 3 cut(s) 442, 504, 604
HphI GGTGA 3 cut(s) 194, 533, 565
Hpy166II GTNNAC 3 cut(s) 328, 393, 645
Hpy188I TCNGA 2 cut(s) 208, 739
Hpy188III TCNNGA 3 cut(s) 304, 406, 508
Hpy8I GTNNAC 3 cut(s) 328, 393, 645
Hpy99I CGWCG 3 cut(s) 326, 344, 553
HpyAV CCTTC 4 cut(s) 31, 202, 241, 358
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 4 cut(s) 6, 75, 324, 498
HpyCH4V TGCA 3 cut(s) 107, 158, 333
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 2 cut(s) 272, 736
HpySE526I ACGT 4 cut(s) 6, 75, 324, 498
Hsp92II CATG 3 cut(s) 69, 409, 662
HspAI GCGC 3 cut(s) 59, 399, 665
Kzo9I GATC 2 cut(s) 336, 739
LguI GCTCTTC 1 cut(s) 554
LmnI GCTCC 1 cut(s) 18
LpnPI CCDG 9 cut(s) 95, 225, 317, 319, 409, 561, 637, 689, 716
Lsp1109I GCAGC 2 cut(s) 119, 351
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 141
MaeII ACGT 4 cut(s) 6, 75, 324, 498
MaeIII GTNAC 2 cut(s) 424, 654
MalI GATC 2 cut(s) 338, 741
MboI GATC 2 cut(s) 336, 739
MboII GAAGA 8 cut(s) 195, 307, 310, 523, 571, 628, 631, 634
MhlI GDGCHC 3 cut(s) 189, 566, 730
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 3 cut(s) 173, 379, 598
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 1 cut(s) 451
MmeI TCCRAC 1 cut(s) 231
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 3 cut(s) 176, 371, 594
MslI CAYNNNNRTG 2 cut(s) 54, 153
Msp20I TGGCCA 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 704
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 78
NdeII GATC 2 cut(s) 336, 739
NlaIII CATG 3 cut(s) 69, 409, 662
NlaIV GGNNCC 2 cut(s) 14, 124
NmuCI GTSAC 2 cut(s) 424, 654
NsbI TGCGCA 1 cut(s) 400
NspI RCATGY 2 cut(s) 69, 662
PagI TCATGA 1 cut(s) 405
PciI ACATGT 2 cut(s) 65, 658
PciSI GCTCTTC 1 cut(s) 554
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 318
PfeI GAWTC 2 cut(s) 504, 604
PkrI GCNGC 2 cut(s) 109, 366
PleI GAGTC 1 cut(s) 450
PpsI GAGTC 1 cut(s) 450
PscI ACATGT 2 cut(s) 65, 658
PshBI ATTAAT 2 cut(s) 176, 371
Psp124BI GAGCTC 1 cut(s) 566
Psp1406I AACGTT 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 702
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 2 cut(s) 14, 124
PspPI GGNCC 1 cut(s) 706
PstI CTGCAG 1 cut(s) 335
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
RseI CAYNNNNRTG 2 cut(s) 54, 153
SacI GAGCTC 1 cut(s) 566
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 3 cut(s) 176, 371, 594
SatI GCNGC 2 cut(s) 108, 365
Sau3AI GATC 2 cut(s) 336, 739
Sau96I GGNCC 1 cut(s) 706
SchI GAGTC 1 cut(s) 451
ScrFI CCNGG 1 cut(s) 704
SduI GDGCHC 3 cut(s) 189, 566, 730
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 54, 153
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 3 cut(s) 173, 379, 598
SsiI CCGC 7 cut(s) 24, 61, 120, 189, 291, 352, 450
SspMI CTAG 1 cut(s) 141
SstI GAGCTC 1 cut(s) 566
StyD4I CCNGG 1 cut(s) 702
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 4 cut(s) 9, 78, 327, 501
TaqI TCGA 3 cut(s) 151, 234, 321
TasI AATT 3 cut(s) 173, 379, 598
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 2 cut(s) 504, 604
Tru1I TTAA 3 cut(s) 176, 371, 594
Tru9I TTAA 3 cut(s) 176, 371, 594
TseFI GTSAC 2 cut(s) 424, 654
TseI GCWGC 2 cut(s) 107, 364
Tsp45I GTSAC 2 cut(s) 424, 654
TspDTI ATGAA 1 cut(s) 373
VspI ATTAAT 2 cut(s) 176, 371
XceI RCATGY 2 cut(s) 69, 662
XmaJI CCTAGG 1 cut(s) 140
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.