MD04G1038000.v1.1

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005704
Physical Location & Seq
Forward (+)
4227636 .. 4233186
5551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1038000.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGAACTCCTCCACCCCCGCTCACGCTCTCACCACTCACTCTCCACTCCACCGCTTCACCACCGCCACTGTCGCCGCCCCATGTCAGTCCTCCTTCTCCTGCTCCATGCCCCGTCCCCCCAATCGCAACCGCAGAACTCGCCGCAATCGAAACACCACCCGCCCCACCACCACCACCACCACCACCTCCACCACCATTAAACCCTATTTCTACGCTCCTTCACCATCCAACCCTGATACTCTCCAAGCCACCTTCGACCTCGATTACATCTACCACACCTCCCACTCCACCCTCAACCAATTCCTCTCCGACTCCTCCGACGCCCTCCAAGACTTCCGCACTCTCGTATCTGTCGACGCCGGTAATCGCGTCATCGTCTCCTGTCGCCCTTCCACGCTGCGTTTCGTCGGCAATTTGGTGATTATCACCTTCTCCGTCGTTTTGGGGTTTAGGGTTTTGGTTGCCTTGGTCAGGTTAGGGTTTAGGAGTCAGTCCGGGTATGGCAAGGAGGCGACGGTGGTGAGGCGGGATAGGAGTCTCGGGGGAAAAGAGGTTGTGGTAGGGAGAGTGGAGAAGGAGAGAGTTGATGCGAGGAAAAGAAAGAGTTTTGGGATTTTGGATAATCCGCTGTCAATGCCGAAGAGTAGGATGGTGGATGGTTTGGGGAGGATGTTAAAGAGTCAGGTTCGGTGGGCGAAGAAGCTGCCCAGCTGGTGGCCGAGCTCAGCGCCGCAGCAGAGTCTGGTGGTGGATAAGGAGTACTACCAGGGAGAAGCAGACAGATTGGTTAGAGTGATCACAGACAATAGAATGAGCGGAAAGGACATTGGAGAGGATGACATTATTCATTTACGTCAAATATGCAGGACATCTGGAGTACGGGTCTCCTTTGATACAACAAATACACGAGATTCGTTCTACCGTGTGTCTGTTGAATATGTCTTAAATGTTTGCAGCAGGGCCCCAAGTCATTCTACTTATGTTCAGATTGACGGAGAAGATGTTTGTCAATTCATTGCTGGGCTTGCTGGGAATATTGGGCTTGACAACATTCGTGCTGCAAGAATTATGTGTGCAGCAGTGGCTGCACGTACGCGCTCACGTTTTCTGCAGGCGTGGGCATATGCATTGGTAATGCAAAGTAAACATGCAGAAGCACTAGTCGAACTGTCAAAGATATGCCTGCTTTTGCGGATATTCCCTCCCGAGGAGTCCTCTCCTGAGATGGAGATGGTTGCTCGAGGCTTGGAAAAACATTTGAAATTGGATCAGAGGGAATTTCTAATGAGCATGCTCACCGGCGTTTGCAGTGAAAAAAGCCAAAGAAGAGCAGCAGAAGCTTTGGGTTTGTTGGCAGCAACCTCGACCAACAATTTTTGGGGCTGGACGGGGAAATCATGGATGCCGTGTAGACTACCCATACGGGGAGACAGACTAAACTTTCTAGCTGAAAGTGAAAGATGCGATCTTCGAGAGGTCTTGGCGATGATGATGAACAATAAAAGTAGTTACTGGGTAAATAATCATCAGCAAGTAAGGTTGTGTATTTACATCTGTGATTCAATTCGAATCACCCACCAAGAAGAAACACATCGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

534

Amino Acids

60.22

Weight (kDa)

9.71

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 714
AccBSI CCGCTC 2 cut(s) 20, 816
AccI GTMKAC 2 cut(s) 354, 1411
AccII CGCG 2 cut(s) 369, 1096
AclWI GGATC 1 cut(s) 1275
AcoI YGGCCR 1 cut(s) 716
AcsI RAATTY 1 cut(s) 1277
AcyI GRCGYC 2 cut(s) 321, 357
AfaI GTAC 3 cut(s) 761, 879, 1093
AfiI CCNNNNNNNGG 4 cut(s) 471, 714, 1207, 1424
AgsI TTSAA 3 cut(s) 935, 1261, 1563
AhlI ACTAGT 1 cut(s) 1159
AjnI CCWGG 1 cut(s) 765
AluBI AGCT 5 cut(s) 703, 711, 723, 1340, 1448
AluI AGCT 5 cut(s) 703, 711, 723, 1340, 1448
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 4 cut(s) 382, 542, 889, 1422
AlwI GGATC 1 cut(s) 1275
AlwNI CAGNNNCTG 2 cut(s) 742, 1085
Ama87I CYCGRG 3 cut(s) 539, 1205, 1239
AoxI GGCC 2 cut(s) 716, 960
ApaI GGGCCC 1 cut(s) 964
ApeKI GCWGC 9 cut(s) 397, 703, 733, 954, 1058, 1076, 1085, 1331, 1355
ApoI RAATTY 1 cut(s) 1277
AspLEI GCGC 2 cut(s) 730, 1098
AspS9I GGNCC 2 cut(s) 960, 961
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 8 cut(s) 22, 49, 213, 418, 430, 532, 1288, 1564
AsuII TTCGAA 1 cut(s) 1567
AvaI CYCGRG 3 cut(s) 539, 1205, 1239
BaeGI GKGCMC 1 cut(s) 964
BaeI ACNNNNGTAYC 2 cut(s) 330, 363
BanII GRGCYC 2 cut(s) 725, 964
BauI CACGAG 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 9 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1343, 1367
BccI CCATC 5 cut(s) 232, 643, 650, 1219, 1225
BceAI ACGGC 1 cut(s) 1390
BcgI CGANNNNNNTGC 4 cut(s) 685, 719, 1344, 1378
BciT130I CCWGG 1 cut(s) 767
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 4 cut(s) 382, 542, 889, 1422
BcuI ACTAGT 1 cut(s) 1159
BfaI CTAG 2 cut(s) 1160, 1445
BfmI CTRYAG 1 cut(s) 1109
BfoI RGCGCY 1 cut(s) 731
BlpI GCTNAGC 1 cut(s) 724
BmcAI AGTACT 1 cut(s) 761
Bme1390I CCNGG 2 cut(s) 496, 767
BmeT110I CYCGRG 3 cut(s) 539, 1205, 1239
BmgT120I GGNCC 2 cut(s) 960, 961
BmiI GGNNCC 2 cut(s) 962, 963
BmrFI CCNGG 2 cut(s) 496, 767
BmrI ACTGGG 1 cut(s) 1522
BmsI GCATC 3 cut(s) 577, 1392, 1451
BmuI ACTGGG 1 cut(s) 1522
BplI GAGNNNNNCTC 2 cut(s) 1199, 1231
BpmI CTGGAG 1 cut(s) 894
Bpu1102I GCTNAGC 1 cut(s) 724
Bpu14I TTCGAA 1 cut(s) 1567
BpuMI CCSGG 1 cut(s) 496
BsaAI YACGTR 1 cut(s) 1091
BsaHI GRCGYC 2 cut(s) 321, 357
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 3 cut(s) 465, 766, 1206
BsaXI ACNNNNNCTCC 8 cut(s) 25, 55, 263, 293, 867, 897, 987, 1017
Bsc4I CCNNNNNNNGG 4 cut(s) 471, 714, 1207, 1424
Bse118I RCCGGY 2 cut(s) 359, 1298
Bse1I ACTGG 1 cut(s) 1517
Bse3DI GCAATG 1 cut(s) 1014
BseBI CCWGG 1 cut(s) 767
BseDI CCNNGG 3 cut(s) 465, 766, 1206
BseGI GGATG 6 cut(s) 224, 654, 661, 675, 841, 1407
BseLI CCNNNNNNNGG 4 cut(s) 471, 714, 1207, 1424
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 2 cut(s) 738, 1212
BseNI ACTGG 1 cut(s) 1517
BseRI GAGGAG 2 cut(s) 304, 1223
BseSI GKGCMC 1 cut(s) 964
BseXI GCAGC 9 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1343, 1367
BseYI CCCAGC 3 cut(s) 707, 1019, 1028
BsgI GTGCAG 2 cut(s) 1071, 1095
Bsh1236I CGCG 2 cut(s) 369, 1096
BshFI GGCC 2 cut(s) 718, 962
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 3 cut(s) 539, 1205, 1239
BsiSI CCGG 3 cut(s) 360, 495, 1299
BsiWI CGTACG 1 cut(s) 1091
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 4 cut(s) 471, 714, 1207, 1424
BsmAI GTCTC 4 cut(s) 382, 542, 889, 1422
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 2 cut(s) 718, 962
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 3 cut(s) 539, 1205, 1239
Bsp119I TTCGAA 1 cut(s) 1567
Bsp120I GGGCCC 1 cut(s) 960
Bsp1286I GDGCHC 2 cut(s) 725, 964
Bsp143I GATC 3 cut(s) 795, 1267, 1465
Bsp1720I GCTNAGC 1 cut(s) 724
BspANI GGCC 2 cut(s) 718, 962
BspCNI CTCAG 2 cut(s) 737, 1213
BspFNI CGCG 2 cut(s) 369, 1096
BspLI GGNNCC 2 cut(s) 962, 963
BspMAI CTGCAG 1 cut(s) 1113
BspPI GGATC 1 cut(s) 1275
BspQI GCTCTTC 1 cut(s) 1321
BspT104I TTCGAA 1 cut(s) 1567
BspTNI GGTCTC 1 cut(s) 889
BsrBI CCGCTC 2 cut(s) 20, 816
BsrDI GCAATG 1 cut(s) 1014
BsrFI RCCGGY 2 cut(s) 359, 1298
BsrI ACTGG 1 cut(s) 1517
BssAI RCCGGY 2 cut(s) 359, 1298
BssECI CCNNGG 3 cut(s) 465, 766, 1206
BssMI GATC 3 cut(s) 795, 1267, 1465
BssNI GRCGYC 2 cut(s) 321, 357
BssSI CACGAG 1 cut(s) 906
BssT1I CCWWGG 1 cut(s) 465
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 4 cut(s) 70, 517, 923, 1170
Bst6I CTCTTC 2 cut(s) 635, 1321
BstACI GRCGYC 2 cut(s) 321, 357
BstAPI GCANNNNNTGC 1 cut(s) 1085
BstBAI YACGTR 1 cut(s) 1091
BstBI TTCGAA 1 cut(s) 1567
BstC8I GCNNGC 4 cut(s) 1026, 1113, 1184, 1292
BstDEI CTNAG 2 cut(s) 724, 1221
BstENI CCTNNNNNAGG 1 cut(s) 469
BstF5I GGATG 6 cut(s) 224, 654, 661, 675, 841, 1407
BstFNI CGCG 2 cut(s) 369, 1096
BstH2I RGCGCY 1 cut(s) 731
BstHHI GCGC 2 cut(s) 730, 1098
BstKTI GATC 3 cut(s) 798, 1270, 1468
BstMAI GTCTC 4 cut(s) 382, 542, 889, 1422
BstMBI GATC 3 cut(s) 795, 1267, 1465
BstMWI GCNNNNNNNGC 7 cut(s) 71, 138, 634, 1025, 1082, 1085, 1337
BstNI CCWGG 1 cut(s) 767
BstNSI RCATGY 2 cut(s) 1151, 1294
BstSCI CCNGG 2 cut(s) 494, 765
BstSFI CTRYAG 1 cut(s) 1109
BstSLI GKGCMC 1 cut(s) 964
BstUI CGCG 2 cut(s) 369, 1096
BstV1I GCAGC 9 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1343, 1367
BsuRI GGCC 2 cut(s) 718, 962
BtgZI GCGATG 1 cut(s) 1499
BtsCI GGATG 6 cut(s) 224, 654, 661, 675, 841, 1407
BtsI GCAGTG 2 cut(s) 1086, 1315
BtsIMutI CAGTG 3 cut(s) 66, 1086, 1315
Cac8I GCNNGC 4 cut(s) 1026, 1113, 1184, 1292
CaiI CAGNNNCTG 2 cut(s) 742, 1085
CfoI GCGC 2 cut(s) 730, 1098
Cfr10I RCCGGY 2 cut(s) 359, 1298
Cfr13I GGNCC 2 cut(s) 960, 961
CseI GACGC 3 cut(s) 329, 358, 365
Csp6I GTAC 3 cut(s) 760, 878, 1092
CviAII CATG 5 cut(s) 81, 106, 1148, 1291, 1398
CviQI GTAC 3 cut(s) 760, 878, 1092
DdeI CTNAG 2 cut(s) 724, 1221
DpnI GATC 3 cut(s) 797, 1269, 1467
DpnII GATC 3 cut(s) 795, 1267, 1465
EaeI YGGCCR 1 cut(s) 716
Eam1104I CTCTTC 2 cut(s) 635, 1321
EarI CTCTTC 2 cut(s) 635, 1321
Ecl136II GAGCTC 1 cut(s) 723
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 2 cut(s) 725, 964
Eco31I GGTCTC 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 723
Eco88I CYCGRG 3 cut(s) 539, 1205, 1239
EcoICRI GAGCTC 1 cut(s) 723
EcoNI CCTNNNNNAGG 1 cut(s) 469
EcoO109I RGGNCCY 2 cut(s) 960, 961
EcoRII CCWGG 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 1129
EcoT38I GRGCYC 2 cut(s) 725, 964
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 5 cut(s) 84, 109, 1151, 1294, 1401
FaqI GGGAC 1 cut(s) 99
FatI CATG 5 cut(s) 80, 105, 1147, 1290, 1397
FauI CCCGC 3 cut(s) 25, 167, 519
FauNDI CATATG 1 cut(s) 1123
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 2 cut(s) 354, 1411
FokI GGATG 6 cut(s) 211, 661, 668, 682, 848, 1414
FriOI GRGCYC 2 cut(s) 725, 964
FspBI CTAG 2 cut(s) 1160, 1445
GlaI GCGC 2 cut(s) 729, 1097
GsaI CCCAGC 3 cut(s) 711, 1023, 1032
GsuI CTGGAG 1 cut(s) 894
HaeII RGCGCY 1 cut(s) 731
HaeIII GGCC 2 cut(s) 718, 962
HapII CCGG 3 cut(s) 360, 495, 1299
HgaI GACGC 3 cut(s) 329, 358, 365
HhaI GCGC 2 cut(s) 730, 1098
Hin1I GRCGYC 2 cut(s) 321, 357
Hin1II CATG 5 cut(s) 84, 109, 1151, 1294, 1401
Hin6I GCGC 2 cut(s) 728, 1096
HinP1I GCGC 2 cut(s) 728, 1096
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 1338
HinfI GANTC 9 cut(s) 311, 487, 535, 679, 739, 911, 1211, 1559, 1569
HpaII CCGG 3 cut(s) 360, 495, 1299
HphI GGTGA 8 cut(s) 22, 49, 213, 418, 430, 532, 1288, 1564
Hpy166II GTNNAC 3 cut(s) 355, 1145, 1412
Hpy188I TCNGA 4 cut(s) 310, 319, 987, 1272
Hpy188III TCNNGA 4 cut(s) 873, 1205, 1220, 1472
Hpy8I GTNNAC 3 cut(s) 355, 1145, 1412
Hpy99I CGWCG 6 cut(s) 323, 359, 410, 440, 517, 1599
HpyAV CCTTC 6 cut(s) 103, 228, 262, 399, 439, 568
HpyCH4III ACNGT 4 cut(s) 70, 517, 923, 1170
HpyCH4IV ACGT 3 cut(s) 853, 1090, 1102
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 138, 634, 1025, 1082, 1085, 1337
HpyF3I CTNAG 2 cut(s) 724, 1221
HpySE526I ACGT 3 cut(s) 853, 1090, 1102
Hsp92I GRCGYC 2 cut(s) 321, 357
Hsp92II CATG 5 cut(s) 84, 109, 1151, 1294, 1401
HspAI GCGC 2 cut(s) 728, 1096
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 3 cut(s) 795, 1267, 1465
LguI GCTCTTC 1 cut(s) 1321
LmnI GCTCC 2 cut(s) 107, 220
Lsp1109I GCAGC 9 cut(s) 384, 690, 745, 966, 1045, 1072, 1088, 1343, 1367
LweI GCATC 3 cut(s) 577, 1392, 1451
MaeI CTAG 2 cut(s) 1160, 1445
MaeII ACGT 3 cut(s) 853, 1090, 1102
MaeIII GTNAC 1 cut(s) 1508
MalI GATC 3 cut(s) 797, 1269, 1467
MbiI CCGCTC 2 cut(s) 20, 816
MboI GATC 3 cut(s) 795, 1267, 1465
MboII GAAGA 6 cut(s) 652, 709, 1010, 1338, 1460, 1595
MhlI GDGCHC 2 cut(s) 725, 964
MluCI AATT 8 cut(s) 299, 412, 1010, 1065, 1262, 1277, 1372, 1563
MlyI GAGTC 6 cut(s) 305, 496, 544, 688, 748, 1220
MmeI TCCRAC 3 cut(s) 252, 333, 342
Mph1103I ATGCAT 1 cut(s) 1129
MseI TTAA 3 cut(s) 198, 674, 944
MspA1I CMGCKG 2 cut(s) 628, 711
MspI CCGG 3 cut(s) 360, 495, 1299
MspR9I CCNGG 2 cut(s) 496, 767
MvaI CCWGG 1 cut(s) 767
MvnI CGCG 2 cut(s) 369, 1096
MwoI GCNNNNNNNGC 7 cut(s) 71, 138, 634, 1025, 1082, 1085, 1337
NciI CCSGG 1 cut(s) 496
NdeI CATATG 1 cut(s) 1123
NdeII GATC 3 cut(s) 795, 1267, 1465
NlaIII CATG 5 cut(s) 84, 109, 1151, 1294, 1401
NlaIV GGNNCC 2 cut(s) 962, 963
NmeAIII GCCGAG 1 cut(s) 744
NsiI ATGCAT 1 cut(s) 1129
NspI RCATGY 2 cut(s) 1151, 1294
NspV TTCGAA 1 cut(s) 1567
PaeI GCATGC 1 cut(s) 1294
PaeR7I CTCGAG 1 cut(s) 1239
PciSI GCTCTTC 1 cut(s) 1321
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 351
PfeI GAWTC 3 cut(s) 911, 1559, 1569
Pfl23II CGTACG 1 cut(s) 1091
PflMI CCANNNNNTGG 1 cut(s) 714
PleI GAGTC 6 cut(s) 305, 495, 543, 687, 747, 1219
PpsI GAGTC 6 cut(s) 305, 495, 543, 687, 747, 1219
Ppu21I YACGTR 1 cut(s) 1091
Psp124BI GAGCTC 1 cut(s) 725
Psp6I CCWGG 1 cut(s) 765
PspFI CCCAGC 3 cut(s) 707, 1019, 1028
PspGI CCWGG 1 cut(s) 765
PspLI CGTACG 1 cut(s) 1091
PspN4I GGNNCC 2 cut(s) 962, 963
PspOMI GGGCCC 1 cut(s) 960
PspPI GGNCC 2 cut(s) 960, 961
PspXI VCTCGAGB 1 cut(s) 1239
PstI CTGCAG 1 cut(s) 1113
PstNI CAGNNNCTG 2 cut(s) 742, 1085
PvuII CAGCTG 1 cut(s) 711
RsaI GTAC 3 cut(s) 761, 879, 1093
RsaNI GTAC 3 cut(s) 760, 878, 1092
SacI GAGCTC 1 cut(s) 725
SalI GTCGAC 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 1321
SaqAI TTAA 3 cut(s) 198, 674, 944
Sau3AI GATC 3 cut(s) 795, 1267, 1465
Sau96I GGNCC 2 cut(s) 960, 961
ScaI AGTACT 1 cut(s) 761
SchI GAGTC 6 cut(s) 305, 496, 544, 688, 748, 1220
ScrFI CCNGG 2 cut(s) 496, 767
SduI GDGCHC 2 cut(s) 725, 964
SfaNI GCATC 3 cut(s) 577, 1392, 1451
SfcI CTRYAG 1 cut(s) 1109
Sfr274I CTCGAG 1 cut(s) 1239
SfuI TTCGAA 1 cut(s) 1567
SgrAI CRCCGGYG 1 cut(s) 1298
SlaI CTCGAG 1 cut(s) 1239
SmlI CTYRAG 1 cut(s) 1239
SmoI CTYRAG 1 cut(s) 1239
SpeI ACTAGT 1 cut(s) 1159
SphI GCATGC 1 cut(s) 1294
Sse9I AATT 8 cut(s) 299, 412, 1010, 1065, 1262, 1277, 1372, 1563
SspI AATATT 1 cut(s) 1036
SspMI CTAG 2 cut(s) 1160, 1445
SstI GAGCTC 1 cut(s) 725
StyD4I CCNGG 2 cut(s) 494, 765
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 4 cut(s) 70, 517, 923, 1170
TaiI ACGT 3 cut(s) 856, 1093, 1105
TaqI TCGA 9 cut(s) 148, 255, 261, 354, 1164, 1240, 1364, 1471, 1567
TasI AATT 8 cut(s) 299, 412, 1010, 1065, 1262, 1277, 1372, 1563
TatI WGTACW 1 cut(s) 759
TauI GCSGC 3 cut(s) 77, 144, 733
TfiI GAWTC 3 cut(s) 911, 1559, 1569
Tru1I TTAA 3 cut(s) 198, 674, 944
Tru9I TTAA 3 cut(s) 198, 674, 944
TscAI CASTG 3 cut(s) 73, 1086, 1315
TseI GCWGC 9 cut(s) 397, 703, 733, 954, 1058, 1076, 1085, 1331, 1355
TspDTI ATGAA 4 cut(s) 17, 836, 1003, 1508
TspGWI ACGGA 2 cut(s) 424, 1008
TspRI CASTG 3 cut(s) 73, 1086, 1315
Van91I CCANNNNNTGG 1 cut(s) 714
XagI CCTNNNNNAGG 1 cut(s) 469
XapI RAATTY 1 cut(s) 1277
XceI RCATGY 2 cut(s) 1151, 1294
XhoI CTCGAG 1 cut(s) 1239
XmiI GTMKAC 2 cut(s) 354, 1411
XspI CTAG 2 cut(s) 1160, 1445
ZrmI AGTACT 1 cut(s) 761
Zsp2I ATGCAT 1 cut(s) 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.