AT3G05010

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004430
Physical Location & Seq
Forward (+)
1389371 .. 1391675
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05010.1

Sequence Viewer

Length: 903 bp
ATGCGAGTGCTCAGCGAGATTGCGGAGTCACCGTTCGTGATTTCTCGGCTAAGCCCTGATTCGACGGCCACCGGAGGGTTTATCGGCGGATGGGTCGGGAAGTGTCATGGGTTTCTTCATAACACGGTTTTGGTTTTAGCATCTATTCTATTCGTCGCTTACTTAGCGTACGAGGCGAAGAAGAGCTTGTCCAAGTTGTCTAATCGGAGATCTTACATTATGATTGCCTATTATGGTTTTCTTTGGCTTGTTAGCTTGCTCAATCTTGCTTGGTGTTGCCTTCAGGCATGGGAATGCACTCCGGGGAAAGAAGTTATTTGGAATTTGTTAACTTTGTTCACAACATCTGGAATGTTGTTTCTGGAAGTAAGCTTGGTAGCCTTTCTCTTCCAAGGAAACTATGCAAGCGGTGCTGAAGCATTAACACGAACTTTCCTCATCTCGGGGCTTGTCATTGGTCTCGATTTGCTTCTCAAGGCAATTTATCTGTTTGGCTTTGGAGTACCATTGTTTATCGACAACAATGAACATATACATAAGTTCAAATGGGGCTTATGGGTCATCCACAAGCTCTTACTAGCTGGCATTTATGGGATGATATTCTTCATGTACAACTCCAAATGGAGAGAGAGATTACCAGCAAGACCTGCATTCTACAAGTATATAACTGTCATGCTCGCCTTAAATGGGCTCTCCCTATTTGCATGTGCTCTTACGGCAAACGGTGCTCATTTTGGATTATGGTTGTATGGGATCACAAGTGTTTGTTACCACGCCTTCTATCTTCCTCTTTTGTATGTTACTTTTCTTGCAGACTTTTTCCAGGAGGAAGATCTGAATTTGGAGAATGTCTACTATTCAGAAATGAAAGATGCTGGATTCTTCGACGCTGATTGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

300

Amino Acids

34.06

Weight (kDa)

6.38

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 34 - 287 6.4e-120 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 655
AccI GTMKAC 1 cut(s) 852
AciI CCGC 3 cut(s) 23, 87, 408
AclWI GGATC 1 cut(s) 761
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 2 cut(s) 322, 838
AcuI CTGAAG 2 cut(s) 266, 435
AfaI GTAC 3 cut(s) 170, 504, 611
AfiI CCNNNNNNNGG 3 cut(s) 75, 442, 687
AgsI TTSAA 1 cut(s) 544
AjnI CCWGG 1 cut(s) 822
AloI GAACNNNNNNTCC 2 cut(s) 17, 49
AluBI AGCT 5 cut(s) 186, 255, 372, 571, 581
AluI AGCT 5 cut(s) 186, 255, 372, 571, 581
Alw21I GWGCWC 3 cut(s) 12, 712, 730
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 442
AoxI GGCC 1 cut(s) 66
ApoI RAATTY 2 cut(s) 322, 838
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 21
AvaI CYCGRG 1 cut(s) 442
BanII GRGCYC 1 cut(s) 693
Bbv12I GWGCWC 3 cut(s) 12, 712, 730
BccI CCATC 1 cut(s) 84
BceAI ACGGC 2 cut(s) 81, 732
BciT130I CCWGG 1 cut(s) 824
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 578
BfuAI ACCTGC 1 cut(s) 655
BglII AGATCT 2 cut(s) 209, 832
BlpI GCTNAGC 2 cut(s) 11, 50
Bme1390I CCNGG 2 cut(s) 303, 824
BmeT110I CYCGRG 1 cut(s) 442
BmrFI CCNGG 2 cut(s) 303, 824
BmsI GCATC 2 cut(s) 149, 862
Bpu1102I GCTNAGC 2 cut(s) 11, 50
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 2 cut(s) 302, 391
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 442, 687
BseBI CCWGG 1 cut(s) 824
BseDI CCNNGG 2 cut(s) 302, 391
BseGI GGATG 3 cut(s) 95, 561, 600
BseLI CCNNNNNNNGG 3 cut(s) 75, 442, 687
BseMII CTCAG 1 cut(s) 25
BshFI GGCC 1 cut(s) 68
BsiHKAI GWGCWC 3 cut(s) 12, 712, 730
BsiHKCI CYCGRG 1 cut(s) 442
BsiSI CCGG 2 cut(s) 72, 302
BsiWI CGTACG 1 cut(s) 168
BslI CCNNNNNNNGG 3 cut(s) 75, 442, 687
BsmAI GTCTC 1 cut(s) 464
BsmI GAATGC 2 cut(s) 299, 650
BsnI GGCC 1 cut(s) 68
Bso31I GGTCTC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 442
Bsp1286I GDGCHC 4 cut(s) 12, 693, 712, 730
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 3 cut(s) 209, 753, 832
Bsp1720I GCTNAGC 2 cut(s) 11, 50
BspACI CCGC 3 cut(s) 23, 87, 408
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 24
BspMI ACCTGC 1 cut(s) 655
BspPI GGATC 1 cut(s) 761
BspQI GCTCTTC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 464
BsrGI TGTACA 1 cut(s) 609
BssECI CCNNGG 2 cut(s) 302, 391
BssMI GATC 3 cut(s) 209, 753, 832
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 824
Bst4CI ACNGT 4 cut(s) 33, 127, 670, 725
Bst6I CTCTTC 2 cut(s) 176, 392
BstAPI GCANNNNNTGC 3 cut(s) 410, 647, 725
BstAUI TGTACA 1 cut(s) 609
BstC8I GCNNGC 4 cut(s) 257, 406, 583, 678
BstDEI CTNAG 3 cut(s) 11, 50, 163
BstF5I GGATG 3 cut(s) 95, 561, 600
BstKTI GATC 3 cut(s) 212, 756, 835
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 3 cut(s) 209, 753, 832
BstMWI GCNNNNNNNGC 6 cut(s) 164, 173, 410, 647, 716, 725
BstNI CCWGG 1 cut(s) 824
BstNSI RCATGY 1 cut(s) 708
BstSCI CCNGG 2 cut(s) 301, 822
BstX2I RGATCY 2 cut(s) 209, 832
BstYI RGATCY 2 cut(s) 209, 832
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 3 cut(s) 95, 561, 600
BveI ACCTGC 1 cut(s) 655
Cac8I GCNNGC 4 cut(s) 257, 406, 583, 678
CseI GACGC 1 cut(s) 896
Csp6I GTAC 3 cut(s) 169, 503, 610
CviAII CATG 5 cut(s) 107, 288, 607, 673, 705
CviQI GTAC 3 cut(s) 169, 503, 610
DdeI CTNAG 3 cut(s) 11, 50, 163
DpnI GATC 3 cut(s) 211, 755, 834
DpnII GATC 3 cut(s) 209, 753, 832
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 176, 392
EarI CTCTTC 2 cut(s) 176, 392
EciI GGCGGA 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 464
Eco57I CTGAAG 2 cut(s) 266, 435
Eco88I CYCGRG 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 822
EcoT14I CCWWGG 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 5 cut(s) 110, 291, 610, 676, 708
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FatI CATG 5 cut(s) 106, 287, 606, 672, 704
FblI GTMKAC 1 cut(s) 852
FokI GGATG 3 cut(s) 102, 548, 607
FriOI GRGCYC 1 cut(s) 693
FspBI CTAG 1 cut(s) 578
HaeIII GGCC 1 cut(s) 68
HapII CCGG 2 cut(s) 72, 302
HgaI GACGC 1 cut(s) 896
Hin1II CATG 5 cut(s) 110, 291, 610, 676, 708
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 3 cut(s) 26, 59, 879
HpaI GTTAAC 1 cut(s) 330
HpaII CCGG 2 cut(s) 72, 302
HphI GGTGA 1 cut(s) 21
Hpy166II GTNNAC 3 cut(s) 330, 339, 853
Hpy188I TCNGA 3 cut(s) 207, 837, 862
Hpy188III TCNNGA 5 cut(s) 37, 97, 348, 362, 461
Hpy8I GTNNAC 3 cut(s) 330, 339, 853
Hpy99I CGWCG 3 cut(s) 67, 158, 890
HpyAV CCTTC 2 cut(s) 290, 787
HpyCH4III ACNGT 4 cut(s) 33, 127, 670, 725
HpyCH4V TGCA 5 cut(s) 297, 404, 650, 704, 812
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 173, 410, 647, 716, 725
HpyF3I CTNAG 3 cut(s) 11, 50, 163
Hsp92II CATG 5 cut(s) 110, 291, 610, 676, 708
KspAI GTTAAC 1 cut(s) 330
Kzo9I GATC 3 cut(s) 209, 753, 832
LguI GCTCTTC 1 cut(s) 176
LweI GCATC 2 cut(s) 149, 862
MaeI CTAG 1 cut(s) 578
MaeIII GTNAC 3 cut(s) 27, 767, 799
MalI GATC 3 cut(s) 211, 755, 834
MboI GATC 3 cut(s) 209, 753, 832
MboII GAAGA 8 cut(s) 107, 190, 193, 379, 595, 776, 842, 874
MflI RGATCY 2 cut(s) 209, 832
MhlI GDGCHC 4 cut(s) 12, 693, 712, 730
MluCI AATT 3 cut(s) 322, 480, 838
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 5 cut(s) 68, 166, 446, 798, 820
MseI TTAA 3 cut(s) 329, 422, 683
MslI CAYNNNNRTG 1 cut(s) 292
MspI CCGG 2 cut(s) 72, 302
MspR9I CCNGG 2 cut(s) 303, 824
Mva1269I GAATGC 2 cut(s) 299, 650
MvaI CCWGG 1 cut(s) 824
MwoI GCNNNNNNNGC 6 cut(s) 164, 173, 410, 647, 716, 725
NciI CCSGG 1 cut(s) 303
NdeII GATC 3 cut(s) 209, 753, 832
NlaIII CATG 5 cut(s) 110, 291, 610, 676, 708
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 27
NspI RCATGY 1 cut(s) 708
PciSI GCTCTTC 1 cut(s) 176
PctI GAATGC 2 cut(s) 299, 650
PfeI GAWTC 2 cut(s) 59, 879
Pfl23II CGTACG 1 cut(s) 168
PfoI TCCNGGA 1 cut(s) 822
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 822
PspGI CCWGG 1 cut(s) 822
PspLI CGTACG 1 cut(s) 168
PsuI RGATCY 2 cut(s) 209, 832
RsaI GTAC 3 cut(s) 170, 504, 611
RsaNI GTAC 3 cut(s) 169, 503, 610
RseI CAYNNNNRTG 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 3 cut(s) 329, 422, 683
Sau3AI GATC 3 cut(s) 209, 753, 832
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 2 cut(s) 303, 824
SduI GDGCHC 4 cut(s) 12, 693, 712, 730
SetI ASST 6 cut(s) 188, 257, 374, 573, 583, 649
SfaNI GCATC 2 cut(s) 149, 862
SmiMI CAYNNNNRTG 1 cut(s) 292
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 3 cut(s) 322, 480, 838
SsiI CCGC 3 cut(s) 23, 87, 408
SspMI CTAG 1 cut(s) 578
StyD4I CCNGG 2 cut(s) 301, 822
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 4 cut(s) 33, 127, 670, 725
TaqI TCGA 4 cut(s) 62, 462, 516, 885
TasI AATT 3 cut(s) 322, 480, 838
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 2 cut(s) 59, 879
Tru1I TTAA 3 cut(s) 329, 422, 683
Tru9I TTAA 3 cut(s) 329, 422, 683
TseFI GTSAC 1 cut(s) 27
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 4 cut(s) 107, 540, 595, 881
XapI RAATTY 2 cut(s) 322, 838
XceI RCATGY 1 cut(s) 708
XmiI GTMKAC 1 cut(s) 852
XspI CTAG 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.